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Journal : Journal Microbiology Science

IDENTIFIKASI KOMPONEN KIMIA EKSTRAK ETANOL DAUN PACAR KUKU (Lawsonia inermis L.) SEBAGAI ANTIBAKTERI DAN ANTIOKSIDAN Herwin, Herwin; Nurung, Ayyub Harly; Ambon, Nur Intan; Naid, Tadjuddin
Journal Microbiology Science Vol 2, No 1 (2022)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (313.637 KB) | DOI: 10.56711/jms.v2i1.824

Abstract

Prevention of the transmission of covid 19 can be done by implementing the health protocol, namely 3M (wearing masks, washing hands and social distance). Based on research, the use of masks can reduce the risk of transmission of COVID-19 by 45 percent as well as hand washing reduces the risk by 35 percent. WHO recommends washing hands with soap/antiseptic for 20-30 seconds and applying the right steps. Another effort is to maintain a distance of at least 1 meter and avoid crowds. To assist the government in preventing the transmission of covid 19, socialization of the 3M activity was carried out at the Darul Istiqamah Maros Islamic Boarding School which is located on the Maros-Makassar Street, Mandai District, Maros Regency. This location was chosen because Maros was the third highest area with the number of people who are positive for the corona virus in South Sulawesi. Islamic boarding school students are a partner group that is expected to apply the 3M health protocol so as to prevent the transmission of covid 19. The activity is carried out by providing counseling about the importance of implementing 3M, how to implement 3M and also by distributing masks, soap and disinfectants needed to implement health protocols. The activity was carried out well and received a good response from the participants with 33 participants from various classes who were students of the Darul Istiqamah Islamic Boarding School Makassar.
OPTIMIZATION OF TEMPERATURE CELLULOSE PRODUCING BACTERIAL ISOLATES FROM DRAGON FRUIT (Hylocereus polyrhizus) Nurung, Ayyub Harly; Widowati, Fairuz Dyah; Herwin, Herwin; Fitriana, Fitriana
Journal Microbiology Science Vol 1, No 1 (2021)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (317.796 KB) | DOI: 10.56711/jms.v1i1.820

Abstract

Isolation of bacterial cellulose from fruits in traditional market of Makassar have been done. This objective of this research was to determine optimum temperature of isolate bacteria that produce cellulose from Dragon fruit (Hylocereus polyrhizus) using dilution agar method. Isolation of bacteria produced cellulose using Hestrin-Schramm agar (HSA) obtain 5 (five) isolate NB02, NB03, NS01 and NS02. The result of screening activity showed that isolate NB04 has a potential as bacteria produce cellulose. The temperature optimize of NB04 has been done at 25oC and 40oC. Based on research results showed that optimum temperature for NB04 was at 25oC and has highest cellulose content at 1,29 g.
Production of Antibiotics from Endophyte Fungi Isolates of Bidara Plants (Ziziphus mauritina L.) Isolates Codes IFAZ-6, IFBZ-6, IFDZ-8 With Variations in Carbon Sources Fitriana, Fitriana; Nurung, Ayyub Harly; Adnan, Afdina Putri
Journal Microbiology Science Vol 4, No 2 (2024)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v4i2.1149

Abstract

Research has been carried out on antibiotic production from endophytic fungal isolates coded IFAZ-6, IFBZ-6, and IFDZ-8 from bidara plants (Ziziphus mauritina L.) with variations in carbon sources. This research aims to determine antibiotic production with variations in carbon sources. The first stage was the purification of each selected endophytic fungal isolate. The endophytic fungal isolate was inoculated into the fermentation medium using a variety of carbon sources, namely glucose, fructose, and galactose for 21 days. The antibiotic activity was carried out using the agar diffusion method against Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli bacteria. The results obtained showed that the glucose carbon source was better in producing antibiotic compounds than the galactose carbon source in endophytic fungal isolates coded IFAZ-6, the glucose carbon source was better in producing antibiotic compounds than the fructose and galactose sources in endophytic fungal isolates coded IFBZ-6, and the fructose carbon source is better at producing antibiotic compounds than the galactose source in endophytic fungal isolates coded IFDZ-8.
Antibacterial Activity of Endophytic Fungi Isolated from Parang Romang (Boehmeria virgata (G.Forst) Guill) Leaves Against Gastrointestinal Pathogens Rusli, Rusli; Nurung, Ayyub Harly; Sandyna, Muh. Ivan
Journal Microbiology Science Vol 4, No 2 (2024)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v4i2.1081

Abstract

Parang romang (Boehmeria virgata (G.Forst) Guill) is a plant traditionally used for treating various digestive tract infections. However, obtaining bioactive compounds directly from the plant requires significant biomass. Therefore, endophytic fungi, sourced from specific plant parts, are explored for their ability to produce similar bioactive compounds. The research examined the potential antibacterial activity of endophytic fungi isolated from parang romang leaves against common gastrointestinal pathogens. The methods used in was, characterization of endophytic fungal isolates, and antagonistic activity was evaluated using the agar diffusion method. The results showed that the endophytic fungal strains, namely isolates IFBV 6, IFBV 7, and IFBV 10 could inhibit the bacteria Escherichia coli, Salmonella typhi, Shigella dysenteriae, and Vibrio cholera. With an average zone of inhibition for each strains isolate against Esherichia coli respectively 11.53 mm, 10.49 mm, and 14.85 mm; Salmonella typhi 14.47 mm, 8.66 mm, and 15.51 mm; Shigella dysenteriae 22.28 mm, 13.76 mm, and 15.33 mm; and Vibrio cholera 16.91mm, 11.94mm, 15.16mm. In conclusion, isolated endophytic fungal strains hold promise as potential as antibacterialn agents.
Synergistic Antibacterial Effects of Areca Nut Seed (Areca catechu L.) and Binahong leaf (Anredera cordifolia (Ten.) Steenis) Extracts Against Propionibacterium acnes Nuryanti, Siska; Nurung, Ayyub Harly; Saputra, Muh. Satrio Surya; Musfatir, Harvanita; Umaiyah, Ummi Putri
Journal Microbiology Science Vol 4, No 2 (2024)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v4i2.1150

Abstract

Areca nut (Areca catechu L.) seeds has antibacterial properties due to their alkaloid, flavonoid, tannin, saponin, and polyphenol compounds. Binahong leaves (Anredera cordifolia (Ten.) Steenis) contain flavonoids, polyphenols, saponins, and alkaloids that also act as antibacterial agents. This study aimed to assess the antibacterial activity and synergistic effects of combining extracts from areca nut and binahong leaves against Propionibacterium acnes. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of P. acnes were evaluated for the ethanol extracts of areca nut and binahong leaves. The MIC and MBC values 400 ppm and 1.600 for extract ethanol single areca nut and 50.000 ppm and 100.000 ppm for binahong leaves. The MIC and MBC values for the combination 400/12.500 ppm and 1.600/781,25 ppm for areca nut and 50,000/800 ppm and 100,000/100 ppm for binahong leaves, respectively. The synergy between the two extracts was determined using the Fractional Inhibitory Concentration Index (FICI). The FICI value of 1. P. acnes indicates an indifferent effect (1 < FICI ≤ 4).
Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of Water and Ethanol Extractsof Eleutherine palmifolia (L.) Merr. Against Staphylococcus aureus and Psedomonas aureginosa Rusli, Rusli; Nurung, Ayyub Harly; Erwing, Erwing
Journal Microbiology Science Vol 4, No 2 (2024)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v4i2.1086

Abstract

Dayak onion bulbs (Eleutherine palmifolia (L.) Merr.) are indigenous herbal plants found in Indonesia, rich in phytochemical compounds such as alkaloids, glycosides, flavonoids, phenolics, steroids, and tannins. This study investigates the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of water and ethanol extracts of Dayak onion bulbs against the bacteria Staphylococcus aureus and Pseudomonas aeruginosa. The water extract of Dayak onion bulbs was obtained by boiling using water, while the ethanol extract was obtained by maceration using 96% ethanol solvent. MIC and KBM were determined using the agar diffusion method. The results showed that water extract exhibited an MIC of 0.4% and MBC of 1.6% against both Staphylococcus aureus and Pseudomonas aeruginosa. Meanwhile, the ethanol extract demonstrated an MIC of 0.4% against Staphylococcus aureus and Pseudomonas aeruginosa bacteria with an MBC of 0.8% against Staphylococcus aureus bacteria and 1.6% against Pseudomonas aeruginosa bacteria. These findings suggest the potential of Dayak onion bulb extracts as antimicrobial agents against these pathogenic bacteria.
Antibacterial Activity of Endophytic Fungi Isolate from Bidara Plant (Ziziphus mauritiana Lam.) Against Bacteria That Cause Digestive Infections by Agar Diffusion Method Rahmadhani, Paramita; Fitriana, Fitriana; Nurung, Ayyub Harly
Journal Microbiology Science Vol 4, No 2 (2024)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v4i2.1145

Abstract

Isolates of endophytic fungi coded IFAZ-6 derived from bidara plants (Ziziphus mauritiana Lam) has antibacterial abilities. This study determine whether those isolates has antibacterial activity against bacteria Escherichia coli. Isolates of endophytic fungi were purified and fermented for 21 days and the fermentation results were extracted using ethyl acetate solvent. The ethyl acetate extract obtained was carried out with a minimum inhibitory concentration (MIC) and a minimum bactericidal concentration (MBC) as well as activity tests with the agar diffusion method. From the test results, statistical tests were carried out, the highest concentration IFAZ-6 isolate extracts that has highest inhibitory zone was 300,000 ppm in E. coli bacteria but only significantly different to 200.000 ppm.
Antibacterial Activity of Ethanol Extract of Porang Tubers (Amorphophallus muelleri Blume) Against the Growth of Helicobacter pylori ATCC 43504, Shigella dysenteriae ATCC 13313 and Vibrio Cholerae ATCC 14035 Bacteria Nuryanti, Siska; Nurung, Ayyub Harly; Putra, Aryadi Surya
Journal Microbiology Science Vol 5, No 2 (2025): Volume 5(2) 2025
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v5i2.1358

Abstract

Porang tubers (Amorphophallus muelleri Blume) are known as local plants that contain active compounds such as glucomannan, saponins and flavonoids, which are thought to act as antibacterials. This study was conducted to determine the extent of the ability of ethanol extract of porang tubers to inhibit and kill Helicobacter pylori ATCC 43504, Shigella dysenteriae ATCC 13313, and Vibrio cholerae ATCC 14035 bacteria, by looking at the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) values. The extract was made using the maceration method using 96% ethanol, then tested for antibacterial properties using the liquid dilution method for MIC and the drop plate method for MBC. The results showed the MIC value for the three test bacteria at a concentration of 50,000 ppm. Meanwhile, the MBC value was not achieved even at the highest concentration, which was 100,000 ppm. Based on the results of this study, it can be concluded that the antibacterial effectiveness of ethanol extract of porang tubers is still relatively weak on Helicobacter pylori ATCC 43504, Shigella dysenteriae ATCC 13313, and Vibrio cholerae ATCC 14035 bacteria.
Potential of Antibacterial Producing Rhizosphere Bacteria from Porang Roots (Amorphophallus muelleri Blume) against Gastrointestinal Pathogens Nuryanti, Siska; Nurung, Ayyub Harly; Akihito mr, Andi Al Kahfi
Journal Microbiology Science Vol 5, No 2 (2025): Volume 5(2) 2025
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v5i2.1342

Abstract

Increasing antibiotic resistance in gastrointestinal pathogens such as Escherichia coli, Shigella dysenteriae, and Vibrio cholerae encourages the search for new antimicrobial agents. This study aims to evaluate the antibacterial potential of rhizosphere bacteria from porang roots (Amorphophallus muelleri Blume) against the three pathogens through in vitro tests. Isolation was carried out from rhizosphere soil using a serial dilution technique. Eleven isolates were characterized morphologically and tested for antibacterial activity using the agar diffusion method. Two selected isolates (IBRAM-2 and IBRAM-11) were fermented and extracted with ethyl acetate. Antibacterial activity was evaluated using the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) tests with the microdilution and drop plate methods, each with three replications and chloramphenicol as a positive control. The results showed an inhibition zone of 13.2–18.7 mm. The MIC value is 25,000 ppm, while the MBC value ranges from 25,000–50,000 ppm. Although it shows antibacterial activity, the high concentration required indicates weak antibacterial potential.