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All Journal Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Biologi Tropis Jurnal Pengabdian Masyarakat Sains Indonesia (Indonesian Journal Of Science Community Services) Jurnal Ilmiah Profesi Pendidikan Jurnal Pijar MIPA
Dewa Ayu Citra Rasmi
Pendidikan Biologi Universitas Mataram
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Economics, Econometrics & Finance Education Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Mathematics Physics Public Health Social Sciences

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Isolation and Activity Test of Lipolitic Bacteria on Different pH and Temperature Prapti Sedijani; Dewa Ayu Citra Rasmi; Kusmiyati Kusmiyati; Miko Eniarti; Siti Rohimah
Jurnal Biologi Tropis Vol. 22 No. 3 (2022): July - September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v22i3.4552

Abstract

Lipase is an enzyme that can be applied in many industrial fields so it has a good economic prospects. The demand for lipase was still unfulfilled at the time this article was written. The character of the lipase for industrial needs are vary depending on the industry in which the enzyme is used. Temperature and pH are the two factors that most influence enzyme activity. This article reports on bacteria isolated from coconut, avocado, tempeh, and areca nut and their lipolytic activity at pH 7-10, 30-40oC. Isolation was carried out on NA media supplemented with olive oil and Rhodamine B as the activity indicator. Colonies showing clear zones were used in subsequent work. Activity tests were carried out on the isolation medium pH 7-10 and incubated at 30 or 40oC. Characterization of isolates includes morphological and microscopic observations; proteolytic and amylolytic capability. There were 41 isolates showing lipolytic activity, 26, 7, 4, and 4 isolates from coconut, tempeh, avocado, and areca nut, respectively. Five isolates were selected for further work. Activity tests showed that the best isolates at 30oC were K1A1 and K1A2 at pH 7, each of which had an activity index of 9% and 107%. Meanwhile, at 40oC the best activity was shown by K10 pH 8 with an activity index of 59%. Based on Grams staining and microscopic analysis, isolates belong to Gram-positive coccus bacteria. Isolate K1A1 showed proteolytic and amylolytic activity, K1A2 showed high proteolytic activity but not amylolytic, while K10 did not show both activities.
Profil Pemahaman Konsep Pandemi Covid-19 Pada Siswa di SMA Negeri 1 Gerung Handriani Handriani; Lalu Zulkifli; Dewa Ayu Citra Rasmi; AA Sukarso
Jurnal Ilmiah Profesi Pendidikan Vol. 8 No. 1 (2023): Februari
Publisher : Fakultas Keguruan dan Ilmu Pendidikan, Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jipp.v8i1.1080

Abstract

Penerapan protokol kesehatan dapat memutus mata rantai penularan virus Corona-19 sehingga pemahaman mengenai pandemi Covid-19 menjadi sangat penting. Penelitian ini bertujuan untuk mengetahui profil pemahaman konsep pandemi covid-19  pada siswa di SMA Negeri 1 Gerung . jenis penelitian yang akan digunakan adalah penelitian kuantitatif deskriptif. Sampel  dalam penelitian ini adalah siswa kelas X (MIPA dan IPS) dan XI (MIPA dan IPS). Teknik pengambilan sempel dalam penelitian ini adalah multistage cluster random sampling yang dimana setiap tingkatan kelas dipilih 4 kelas untuk mewakili tiap tingkat kelasnya. Instrumen yang digunakan dalam penelitian ini yaitu, angket pernyataan. Berdasarkan hasil penelitian adalah tidak ada siswa di SMAN 1 Gerung yang memiliki pemahaman konsep pandemi covid-19 yang sangat tinggi. Sebanyak 51 siswa yang memiliki pemahaman tinggi mengenai Pandemi Covid-19 (57 %). 38 siswa memiliki pemahaman sedang tentang pandemi covid-19 (42 %), dan hanya sedikit satu siswa yang memiliki pemahaman rendah (1%). Indikator pengetahuan tentang pandemi covid-19, penyebaran virus corona, mekanisme penularan virus corona, pencegahan covid-19, serta dampak dan gejala covid-19 ditemukan tinggi pada siswa SMA Negeri 1 Gerung, menunjukkan  bahwa siswa memahami konsep pandemi covid-19. Hanya indikator mengenai pengobatan covid-19 yang tergolong rendah.
Hubungan Kemampuan Kolaborasi dan Hasil Belajar Biologi Peserta Didik SMAN di Kota Mataram Baiq Marita; Jamaluddin Jamaluddin; Dewa Ayu Citra Rasmi
Jurnal Ilmiah Profesi Pendidikan Vol. 8 No. 3 (2023): Agustus
Publisher : Fakultas Keguruan dan Ilmu Pendidikan, Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jipp.v8i3.1576

Abstract

Kemampuan kolaborasi adalah kemampuan peserta didik bekerjasama dengan orang lain yang saling menguntungkan dalam menyelesaikan suatu permasalahan untuk mencapai tujuan bersama. Penelitian ini bertujuan untuk mengetahui hubungan kemampuan kolaborasi dan hasil belajar biologi peserta didik SMAN di Kota Mataram. Penelitian ini merupakan penelitian deskriptif kuantitatif yang menggunakan metode korelasional. Populasi penelitian adalah peserta didik kelas XI MIPA SMAN di Kota Mataram. Teknik pengambilan sampel menggunakan multistage sampling. Jumlah sampel terpilih adalah 230 peserta didik. Pengumpulan data menggunakan instrumen penelitian yang terdiri dari kuesioner kemampuan kolaborasi, dan tes hasil belajar biologi. Analisis data dilakukan dengan teknik uji Z, diperoleh nilai Z hitung 3,49 lebih besar dari nilai Z Tabel yaitu 1,96. Hasil tersebut menunjukkan bahwa terdapat hubungan yang signifikan antara kemampuan kolaborasi dan hasil belajar biologi peserta didik. Nilai koefisien korelasi (rho hitung) antar kedua variabel positif sebesar 0,202 yang menunjukkan bahwa hubungan antara kemampuan kolaborasi dan hasil belajar biologi peserta didik SMAN di Kota Mataram termasuk korelasi rendah.
Fungal Crude Lipase Enzyme Produced Using the SSF (Solid State Fermentation) Method Increases The Washing Test Performance Prapti Sedijani; Nida'an Khovia; Dewa Ayu Citra Rasmi; Kusmiyati
Jurnal Biologi Tropis Vol. 23 No. 1 (2023): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i1.6046

Abstract

Lipase is an enzyme that commonly used in various industries, including the detergent industry to improve washing performance. Two fungal isolates (Al and Cl) has been reported to be highly active on high pH. This research aims to see the potential of those fungi (crude enzyme) in improving washing performance. Enzymes were produced using the SSF (Solid State Fermentation) method with sugarcane bagasse as a medium. To determine how adding enzymes might affect the effectiveness of washing, studies were conducted. The results show that washing performance increases from 52% (detergent only) to 74% in combination with the crude enzyme. It is concluded that Al has a potential to be involved in biodetergent production. Similar assays with concentrated enzymes are suggested for future studies.
The Effect of Phosphate-Solubilizing Bacteria and IAA Producers from Cactus Rhizosphere on the Germination of Vigna sinensis L. Muhammad Fikri Alfiansyah; Lalu Zulkifli; Dewa Ayu Citra Rasmi
Jurnal Biologi Tropis Vol. 23 No. 3 (2023): July - September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i3.5089

Abstract

This study aims to examine the effect of phosphate-solubilizing bacteria and IAA producers on the germination of Vigna sinensis L. The bacteria were isolated from the cactus rhizosphere in Senteluk Village, Batulayar Subdistrict, West Lombok. The soluble phosphate was measured qualitatively and quantitatively. Qualitative measurement was based on the solubility of P in the growth medium (solid Pikovskaya) with a clear zone indicator around the bacterial colonies. The solubility of P (liquid Pikovskaya) and IAA production were measured using a spectrophotometer at wavelengths of 430 nm and 530 nm, respectively. In vitro testing of the effects of phosphate-solubilizing bacteria and IAA-producing bacteria on the germination of long beans (Vigna sinensis L.), specifically the Parade tavi variety, was conducted by soaking the long bean seeds in the bacterial suspension and then planting them on Murphy agar medium. The plant height, root length, fresh weight, and dry weight were measured and analyzed using One-Way ANOVA. The bacterial characterization included colony morphology, cell shape, and biochemical tests. The research results obtained 8 isolates that could solubilize phosphate in the low category while producing IAA and 2 other isolates that only produced IAA. Quantitative phosphate solubilization showed the highest soluble phosphate on days 4 and 6 of incubation. The highest IAA production (14.25 ppm) was achieved by the rhizosphere bacterial isolate with the code RK7. The results showed that there was an indication of the influence of phosphate solubilizing and IAA-producing bacteria isolated from the rhizosphere of cactus on Vigna sinensis L. germination, although not significantly different. This shows that local bacterial isolates are quite potential as a source of biofertilizer development in the future.
Protease Enzyme Activity of Fungal Isolates from Avocado and Coconut Fleshes on Different pH and Temperature Prapti Sedijani; Dinda Propita Lestari; Dewa Ayu Citra Rasmi; Kusmiyati Kusmiyati
Jurnal Biologi Tropis Vol. 23 No. 4 (2023): October - December
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i4.5603

Abstract

Protease enzymes are widely applied in the industrial field. Protease enzymes have high economic prospects with unmet demand. This article describes protease activity of two fungi isolated from avocados and from coconuts fleshes that previously have shown a high lipolytic activity on pH 7 to pH 10.  The protease test was performed on PDA medium supplemented with 1% of skim milk, and adjusted to pH 7, pH 8 and pH 9 and was incubated at room temperature and at 30°C. Protease activity was determined based on the clear zone around the colony observed on day two – day eight. The results showed that variations in pH and temperature had a significant effect on the protease activity. The highest protease activity of isolate from avocado and from coconut fleshes were observed at pH 7, room temperature and incubated for 24 hours, with a protease activity index of 0.36 and 0.31 respectively.
Isolation and Screening of Amilolytic Bacteria Isolate from Cassava (Manihot utilissima) Prapti Sedijani; Bq. Novi Aprilia; Dewa Ayu Citra Rasmi; Kusmiyati Kusmiyati
Jurnal Biologi Tropis Vol. 23 No. 4 (2023): October - December
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i4.5604

Abstract

Amylase is an enzyme capable of hydrolyzing starch into simpler sugars. The use of amylase enzymes is urgently needed in various industrial fields, therefore the search for sources of amylase enzyme producers continues to be carried out for sources of amylase enzyme producers. Each industrial field has specific requirements for amylase enzymes. This study aims to isolate bacteria capable of producing amylase enzyme and to determine the amylase activity of bacteria isolated from cassava (Manihot utilissima) when incubated at different temperatures (room temperature and 30℃) and pH levels (7, 8, 9, and 10). The research involved bacterial isolation, testing the amylase activity of the isolated amylolytic bacteria at varying pH and temperatures, and characterizing the amylolytic bacteria. The amylolytic bacterial activity was qualitatively measured based on the clear zone's area formed around the colony. From this study 5 isolates showing amylolytic activity were obtained. The amylolytic activity index varied among the isolates, ranging from 0.12 to 0.59. The highest amylolytic activity index (0.59) was performed by S4 isolate on pH 10 medium incubated at 30℃. Microscopic and gram staining analysis suggest that two isolates were gram-negative coccus bacteria and three of them were gram-positive coccobaccilus bacteria.
Co-Authors AA Sukarso Baiq Fitri Raudatul Hikmah Baiq Marita Bq. Novi Aprilia Dadi Setiadi Dinda Propita Lestari Handriani Handriani Husna Tuhura Jamaluddin Jamaluddin Juwita Anisafitri Khairuddin Khairuddin Kusmiyati Kusmiyati Lalu Septiadi Wirandanu Lalu Zulkifli Miko Eniarti Muhammad Fikri Alfiansyah Ni Luh Putu Pebri Artayani . Ni Nyoman Tria Febrina Nida'an Khovia Prapti Sedijani Prapti Sedijani Siti Aisyah Siska Haerani Siti Rohimah Syamsul Bahri Syamsul Bahri Syamsul Bahri Tri Ayu Lestari Tuti Handayeni