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All Journal Journal of the Medical Sciences (Berkala Ilmu Kedokteran) The Indonesian Biomedical Journal Paediatrica Indonesiana Media Dermato-Venereologica Indonesiana Indonesian Journal of Biomedicine and Clinical Sciences
Ery Kus Dwianingsih
Department Of Anatomical Pathology, Faculty Of Medicine, Public Health And Nursing Universitas Gadjah Mada/Dr. Sardjito General Hospital, Yogyakarta, Indonesia
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health

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Journal : The Indonesian Biomedical Journal

 1 
WIF1 Qualitative-Methylation from Peripheral Blood Could Not Be Used as Biomarker for The Risk of Nasopharyngeal Carcinoma or Smoking Behavior in Yogyakarta Panel Aditya Kurniawan; Erika Diana Risanti; Saihas Suhda; Hanggoro Tri Rinonce; Ery Kus Dwianingsih; Jajah Fachiroh
The Indonesian Biomedical Journal Vol 11, No 3 (2019)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v11i3.810

Abstract

BACKGROUND: Tobacco smoking plays an important role in nasopharyngeal carcinoma (NPC) risk. The Wingless-related integration site (WNT) Inhibitory Factor 1 (WIF1) as one of the tumor suppressor gene (TSG), functions to control cell proliferation through suppression of WNT signaling pathway. WIF1 methylation is one of epigenetic mechanisms which causes overactivated of WNT pathway in NPC development. WIF1 methylation from buffy coat related to smoking status and NPC risk is not known yet.METHODS: This was a nested case-control study involving 39 newly diagnosed NPC patients and 40 healthy controls. All subjects were enrolled at Dr. Sardjito Hospital, Yogyakarta. Subjects were male, with known smoking status, duration, and quantity of cigarette consumed. Parallel DNA isolated from buffy coat was used for WIF1 DNA methylation analysis, by using methylation-specific PCR (MSP) method. Chi-square analysis was used to determine distribution of differences among groups. Two tailed p-value≤0.05 was considered as statistically significant.RESULTS: No significant difference between WIF1 methylation of cases and controls (p=0.30), nor smoking habit among smokers (p=0.51) and non-smokers (p=0.43).CONCLUSION: WIF1 methylation from buffy coat could not be used as an NPC marker nor as a smoking behavior marker.KEYWORDS: buffy coat, cigarette smoking, methylation, nasopharyngeal carcinoma, WIF1
Association of Peripheral Blood RASSF1A and CDKN2A Methylation Status with Smoking Behaviour in Nasopharyngeal Carcinoma Erika Diana Risanti; Aditya Kurniawan; Laila Wahyuningsih; Ery Kus Dwianingsih; Hanggoro Tri Rinonce; Jajah Fachiroh
The Indonesian Biomedical Journal Vol 10, No 2 (2018)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v10i2.381

Abstract

BACKGROUND: Hypermethylation of RASSF1A and CDKN2A is one of epigenetic factor underlies nasopharyngeal carcinoma (NPC) development. Smoking behavior as an NPC’s risk factor causes aberrant DNA methylation. RASSF1A and CDKN2A promoter hypermethylation from peripheral blood cells correlates with smoking behavior. The use of body fluids including peripheral blood as a specimen for DNA methylation analyzes are widely developed, as less invasive method compared to the use of tissue biopsy. This study aims to observe the association between RASSF1A and CDKN2A methylation in peripheral blood and smoking behavioramong NPC patients.METHODS: Newly diagnosed NPC subjects were recruited from ear-nose-throat (ENT) outpatient clinic of Dr. Sardjito Hospital, Yogyakarta. DNA from buffycoat of 19 smokers and 20 non-smokers NPC’s patients were isolated. Bisulphite modification was applied to 500 ng of the isolated DNA. The methylation status was detected by MSP (methylation-specific polymerase chain reaction (PCR)). The association between smoking status and promoter hypermethylation was analysis using Chi-Square test.RESULTS: MSP analysis of RASSF1A showed that 68.42% smoker and 75% non-smoker NPC’s patients were methylated. MSP analysis of CDKN2A showed that 21.05% smoker and 25% non-smoker NPC’s patients were methylated. There was no association between smoking behavior with RASSF1A and CDKN2A methylation (p>0.05).CONCLUSION: Statistical analysis showed that smoking behavior is not associated with methylation of RASSF1A and CDKN2A among NPC’s patients.KEYWORDS: DNA methylation, CDKN2A, RASSF1A, Nasopharyngeal carcinoma, Smoking
Clinicopathological Characteristic and Prognostic Significance of Indonesian Triple Negative Breast Cancer Irianiwati Widodo; Ery Kus Dwianingsih; Teguh Aryandono; Soeripto Soeripto
The Indonesian Biomedical Journal Vol 11, No 3 (2019)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v11i3.831

Abstract

BACKGROUND: Triple negative breast cancer (TNBC) is a breast cancer subtype with negative expressions of estrogen receptors (ER), progesterone receptors (PR) and Her-2 protein receptors. This subtype exhibits aggressive behavior and poor prognosis since it is unresponsive to hormonal and Her-2 targeted therapy. The identification of clinicopathological characteristics and their prognostic values will provide guidance in developing effective treatment. This study was performed to analyze the clinicopathological characteristics and prognostic significance in Indonesian TNBC.METHODS: Forty paraffin-embedded tissues of TNBC, stage I to IIIA, dating from 2008 to 2010 in Dr. Sardjito Hospital, were enrolled. Survival follow-up was done from January 2008 to June 2013. The samples were immunostained with ER, PR and Her-2 monoclonal antibodies to determine the TNBC subtypes. The clinicopathological characteristics were statistically analyzed. The survival was analyzed using the Kaplan Meier method. The Cox proportional hazards model was used for multivariate analysis.RESULTS: Mean age of TNBC patients was 51.42±11.72 years old, whereas the mean of tumor size was 5.4±2.92 cm. Lymph node metastasis was found in 70% of cases. Number of locally advanced samples (IIIA) was 37.5%, while moderate to high grade samples were 95%. Number of still alive patients at the end of the study was 55%. Number of patients that still alive up to the end of the study was 45.50%. Lymph node was an independent prognostic factor for survival of TNBC patients as positive status of lymph node increases the death risk to 6 times higher (p=0.02, RR=6.6). However, after being adjusted with age and stage, lymph node status was suggested as a modifier effect for the survival of TNBCs patients (RR=0.19).CONCLUSION: Indonesian TNBC patients were mostly found already with large tumor size, lymph node metastasis, high pathological grade and relatively young age. Lymph node status serves as a modifier effect for the survival of Indonesian TNBC.KEYWORDS: NBC, clinicopathological characteristics, prognostic significance 
Co-Authors Aditya Kurniawan Aditya Kurniawan Andre Stefanus Panggabean Anwar, Sumadi Lukman Arsyad Hakim, Fikar Bob Irsan Bustanul Ardianto Camelia Herdini Claudia Priska Adelin Didik Setyo Heriyanto Dwi Cahyani Ratna Sari Dwi Tirtasari, Faizah Emilia Theresia Erika Diana Risanti Fikar Arsyad Hakim H R Danarto Hakim, Fikar Arsyad Hanggoro Tri Rinonce Heru Pradjatmo Hifdza Faza Felisha I Indrawati Indrawarman Indrawarman Soeroharjo Irianiwati Widodo Jajah Fachiroh, Jajah Krisnugraha, Yeshua Putra Laila Wahyuningsih Linda Pratiwi Linda Pratiwi Lubis, Anggiat Pratama Mardiah Suci Hardianti Meydita Fuzia Putri Insani Moh Nailul Fahmi Naomi Yoshuantari Nita Damayanti Sulistianingrum Prakoso, Ragil Unggul Raden Danarto Rahadyan Magetsari Rheza Gandi Bawono Rusdy Ghazali Malueka Sagung Rai Indrasari Saihas Suhda Sakti, Yudha Mathan Soeripto Soeripto Solichin, Muchamad Ridotu Sumpono, Auliya Suluk Brilliant Sunardi Radiono Teguh Aryandono Wahyu Tri Widayati Yohanes Widodo Yulia Eka Irmawati Yuni Artha Prabowo Putro Yurisal Akhmad Dany