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All Journal Biofarmasetikal Tropis (The Tropical Journal of Biopharmaceutical)
Jabes W. Kanter
Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Christian University of Indonesia in Tomohon
Biochemistry, Genetics & Molecular Biology Chemistry Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

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Antibacterial Activity Test of Ethanol Extract of Sonneratia alba On Escherichia coli And Staphylococcus aureus kevin wong; Jeane Mongi; Sonny D. Untu; Jabes W. Kanter; Hanna M. Rumagit; Nerni O. Potalangi
Biofarmasetikal Tropis (The Tropical Journal of Biopharmaceutical) Vol. 8 No. 1 (2025): April 2025
Publisher : FMIPA UKIT

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55724/jbiofartrop.v8i1.426

Abstract

Antibacterial is a compound used to control the growth of harmful bacteria. Mangrove plants are strongly suspected to have bioactive content, the bioactive content contained in mangrove plants is useful in the pharmaceutical field as a medicinal ingredient. Sonneratia alba root contains secondary metabolite compounds Flavanoids, tannins and alkaloids that act as antibacterials. The purpose of this study was to determine the antibacterial activity of S.alba ethanol extract against Staphylococcus aureus bacteria and Escherichia coli bacteria. Extraction method by maceration, long extraction time 3 days. The extract obtained is then evaporated solvent in the rotary evaporator then the extract results are made concentrations of 25?g, 50?g, 100?g, and 150?g. antibacterial testing using the Kirby-Bauer method. The results showed that at concentrations of 25?g, 50?g, 100?g, and 150?g Sonneratia alba mangrove roots had antibacterial activity. But those that have strong antibacterial activity in Escherichia coli bacteria are at a concentration of 50? with while in Staphylococcus aureus bacteria are at a concentration of 150?g with an inhibitory zone of 18.6mm, and in Escherichia coli bacteria with an inhibitory zone of 18,3mm.
Antibacterial Activity of Ethanol Extract from Chromolaena odorata L. Against Staphylococcus aureus Bacteria Reski Anti; Jabes W. Kanter; Wilmar Maarisit; Reky R. Palandi; Nancy C. Pelealu; Friska M. Montolalu
Biofarmasetikal Tropis (The Tropical Journal of Biopharmaceutical) Vol. 8 No. 1 (2025): April 2025
Publisher : FMIPA UKIT

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55724/jbiofartrop.v8i1.451

Abstract

Chromolaena odorata L. has bioactive compounds that can be used as raw medicine materials. Bioactive compounds from Chromolaena odorata L. are reported to have pharmacological and antibacterial properties. This study aims to  the antibacterial activity. The antibacterial activity testing used the agar diffusion method using paper discs with 25, 50, 75, 100, and 125 µg/disc extract concentrations. Based on the results obtained, the ethanol extract of Chromolaena odorata L. has antibacterial activity of S. aureus with inhibition zone diameters of 12.14, 11.99, 12.63, 12.62, and 13.85 mm.
Inhibitory Test of Scleria sumatrensis Stem Extract against Propionibacterium acnes Bacteria in Vitro Sonny D. Untu; Douglas N. Pareta; Selvana S. Tulandi; Jabes W. Kanter; Andri Natanel
Biofarmasetikal Tropis (The Tropical Journal of Biopharmaceutical) Vol. 8 No. 1 (2025): April 2025
Publisher : FMIPA UKIT

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55724/jbiofartrop.v8i1.505

Abstract

This study aims to test the antibacterial activity of Scleria sumatrensis extract against Propionibacterium acnes, a bacteria that causes acne. Extraction was carried out using the maceration method using 96% ethanol, and the antibacterial activity test was carried out using the diffusion method using a concentration of 100–500 ?g/disc. The results showed that the extract provided moderate antibacterial activity at a concentration of 100 ?g/disc and increased to strong at a concentration of 200–500 ?g/disc. S. sumatrensis extract contains secondary metabolite compounds such as phenolics, flavonoids, alkaloids, tannins, steroids, and terpenoids that play a role in inhibiting the growth of P. acnes through various mechanisms, including cell membrane damage, inhibition of protein synthesis, and disruption of bacterial metabolism. Based on the results of the study, S. sumatrensis extract has the potential as a natural antibacterial alternative for acne treatment without significant side effects or the risk of antibiotic resistance.
Analysis Of Rhodamine B Dye Content In Lipstick At Beriman Tomohon Market Friska M. Montolalu; Christien Kiroh; Wilmar Maarisit; Jabes W. Kanter; Andry Natanel; Amal R. Ginting; Rachmat Santoso
Biofarmasetikal Tropis (The Tropical Journal of Biopharmaceutical) Vol. 8 No. 2 (2025): OKTOBER 2025
Publisher : FMIPA UKIT

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55724/jbiofartrop.v8i2.521

Abstract

The use of cosmetics such as lipstick aims to improve the natural appearance of the lips and induce discoloration. A problem that is often worrying about today is the presence of prohibited dyes in lipstick preparations' main components. Rhodamine B is a synthetic dye prohibited in cosmetics, especially lipsticks. This research aims to analyze the Rhodamine B dye in lipsticks that do not have a distribution permit from BPOM circulating in the Beriman Tomohon market.The research method was Thin Layer Chromatography (TLC), followed by wavelength measurement on UV-Vis Spectrophotometry. The research population was all red lipstick preparations and 10 (ten) different lipsticks as a sample.Data analysis in this research is to find the RF value obtained from TLC then make a graph of the equation y = bx + a then look for the x value to measure the Rhodamine B content in lipstick. The results of this research were obtained from 1 (one) sample of lipstick label L(I) positive containing Rhodamine B. Visually, the lipstick of the L(I) label has a pink color and fluorescence orange when irradiated with 366nm UV, Rhodamine B has an Rf value of 0.85. In contrast, the L(I) sample has an Rf value of 0.84. Rhodamine B has a wavelength of 544nm, and the lipstick sample L(I) has an absorbance of 0.362 which has a Rhodamine B content of 0.4115%.
Co-Authors Amal R. Ginting Andri Natanel Andry Natanel Christien Kiroh Douglas N. Pareta Friska M. Montolalu Hanna M. Rumagit Jeane Mongi kevin wong Nancy C. Pelealu Nerni O. Potalangi Rachmat Santoso Reky R. Palandi Reski Anti Selvana S. Tulandi Sonny D. Untu Wilmar Maarisit