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Medical Laboratory Technology Journal

JAK
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Published by Poltekkes Kemenkes Banjarmasin

ISSN : - EISSN : 24610879 DOI : https://doi.org/10.31964/mltj

Core Subject : Health, Science,

Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Nursing Public Health

The aim of this journal publication is to disseminate the conceptual thoughts or ideas and research results that have achieved in the area of medical laboratory sciences. This Journal particularly focuses on the main problems in the development of the sciences of medical laboratory areas. It covers the parasitology, bacteriology, virology, hematology, clinical chemistry, toxicology, food and drink chemistry, and any sciences that cover sciences of medical laboratory area.

Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Mikrobiologi dan Bioteknologi Terapan

Articles 10 Documents

Search results for , issue "Vol. 8 No. 2 (2022): December" : 10 Documents clear

Effect of Papaya Leaf Extract Gel (Carica papaya) on Incision Wound Healing in Rattus norvegicus Iin Tri Marlinawati; Siti Nurhidayah; Sanarto Santoso; Yahya Irwanto
Medical Laboratory Technology Journal Vol. 8 No. 2 (2022): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Carica papaya is one kind of herbal plant that can help the wound healing process. Scientifically, the leaves of papaya are proven to have anti-inflammatory effects. Flavonoid, alkaloid, tannin, and saponin compounds in papaya leaves have antimicrobial and antioxidant properties to accelerate the re-epithelization and cell migration needed for wound healing. This research aims to determine the effect of papaya leaf extract gel (Carica papaya) on the healing of incision wounds in Rattus norvegicus based on changes in wound morphology (signs of redness, edema, pus, granulation, moisture, and wound length). This research used female Rattus norvegicus aged 12-16 weeks weighing 180-200 grams, divided into five groups (NaCl 0,9%, povidone-iodine, papaya leaf extract gel with concentrations of 10%, 20%, and 30%). Incision wounds on the back of the rat with a length of 2 cm and depth of 1 cm. The normality test used the Shapiro-Wilk test, and the homogeneity test used the Levene test. The research data were not normally distributed nor homogeneous, so they were analyzed using the Kruskal Wallis and Mann-Whitney tests. The results showed that the extract of papaya leaves with concentrations of 10%, 20%, and 30% had significant differences in positive and negative control groups in the wound healing process with the differences in redness (p=0.038), granulation tissue (p=0.039), moistures (0,48), and wound length (p=0,049). It can be concluded that papaya leaf extract gel (Carica papaya) is effective in accelerating the healing process of incision wounds in Rattus norwegicus at concentrations of 10%, 20%, and 30%, so for further research, it is hoped that a histopathological examination can be carried out so that changes that occur in collagen, neutrophile cells, monocytes, and lymphocytes are seen.

Effect of Time and Temperature Variables on Prothrombin Time and International Normalized Ratio of Plasma in Patients in Gampaha, Sri Lanka Vıraj Ranasınghe; Sameera Maduranga Jayasundara; Sanjaya De Sılva; Faiz Marikar; Randıka Wımalasırı Yapa
Medical Laboratory Technology Journal Vol. 8 No. 2 (2022): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Generating accurate results for Prothrombin time (PT) and International Normalized Ratio (INR), PT/INR test is paramount in treatment monitoring. The diagnosis process measures how long it takes blood to clot. The main objective of this study was to determine the effect of storage time and temperature variables on PT and INR results in patients attending Hematologic clinics in Sri Lanka. This cross-sectional study was conducted during June and July 2021. PT was measured in the plasma samples, stored at different temperatures at baseline (0 hours), and then after 12 hours, 18 hours, and 24 hours after specimen collection. INR was calculated from each result, and the deviation percentage of PT and INR was calculated compared to the baseline result (0 hours). Then PT and INR percentage deviation is grouped as <10% and >10% to find biologically significant variations while paired t-test was performed to find statistical significance using GraphPad prism 8.4.3. At refrigerated temperature, both PT and INR results showed>10% variation once kept 24 hours, and this variation was statistically significant (p<0.01, Wilcoxon matched paired sign ranked test). The plasma specimens kept at laboratory temperature and specimen collection room temperature for 18 hours indicated >10% variation of PT and INR values, while these variations were significant at the same criteria above. These results suggest that the separated plasma could only be recommended to test up to 12 hours. Therefore, the laboratories that operate in the same climatic conditions can now use these results to plan the specimen collection accordingly.

Potential Benefit of Flavonoid in Papaya Leave Gel in Neutrophil, Angiogenesis, and Wound size in Rattus Norvegicus Siti Nurhidayah; Iin Tri Marlinawati; Sri Winarsih; Tatit Nurseta
Medical Laboratory Technology Journal Vol. 8 No. 2 (2022): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

An incision wound is the destruction of several cells in the skin area that has experienced an incision. Several factors influencing wound healing are neutrophils as causative agents of inflammation, angiogenesis, and wound closure. These three factors should undergo physiologically to obtain the optimal healing process. Currently, herbal products are preferred because they have lower side effects than synthetic ones. Papaya leaves contain flavonoids with various functions, including wound closure and anti-inflammatory and antioxidant effects. Topical intervention, especially gel, is good for healing because it can keep the wound moist. This research aimed to determine the effect of the administration of papaya leaf gel on wound closure, neutrophil, and angiogenesis. Thirty white rats were used as the model animals in a randomized experimental study divided into five groups comprising negative control (0,9% NaCl), positive control (10% Povidone-iodine), and three intervention groups treated with gel of Carica papaya leaves at a dosage 10%, 20%, and 30%. All groups were topically administered treatment once a day on the dorsal surface for seven days. Carica papaya leaves contain flavonoids, saponin, tannin, and alkaloids qualitatively and 7,734 mg/kg of the flavonoid quantitatively. There were no significant differences in wound size in all three groups on three days, but Carica papaya leave gel had a superior effect compared to 10% PI and 0,9% NaCl in the wound closure process. There were significant differences in all groups in neutrophil and angiogenesis. Nevertheless, 20% papaya leave gel showed the lowest number of neutrophils and had a superior effect on improving the new blood vessels significantly compared to negative and positive groups.

Effect of Diabetes Mellitus on Hypogonadism in Chronic Renal Failure Savas Karatas; Sennur Kose
Medical Laboratory Technology Journal Vol. 8 No. 2 (2022): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Hypogonadism in male patients with chronic renal failure and diabetes mellitus has been known separately; up to now, the effect of these two diseases together on testosterone deficiency and the impact of testosterone deficiency on metabolic values have not been known precisely, therefore in this study we aimed to investigate testosterone deficiency in patients with diabetes mellitus and chronic renal failure. Eighty-seven chronic renal failure patients and 45 control patients who were followed at endocrinology and nephrology clinics were included in the study. After exclusion criteria, the patients were divided into two groups according to diabetes mellitus status. Groups were compared according to testosterone levels. Testosterone deficiency and good groups were compared to blood glucose, Hba1c, and lipid profile levels. The mean age of 87 CRF patients and 45 people in the control group were similar (59.85 ± 9.99 and 56.67 ± 8.56, respectively, p= 0.16). Testosterone deficiency was 24.1% (21/87) in CRF group and 8.8% (4/45) in control group (p= 0.04). The total testosterone levels were notably lower in the diabetic-CRF patients, 3.44 ± 1.3 vs. 4.26 ± 1.46 mg/dl (p= 0.02). The testosterone deficient CRF group had higher blood glucose and HbA1c according to the testosterone sufficient group. (161.20 ± 61.24 mg/dl vs 133.25 ± 59.87 mg/dl blood glucose, p= 0.04 and 7.54 ± 1.46 vs 6.79 ± 1.14 % HbA1c, p= 0.04). Serum triglyceride and LDL levels did not significantly change between groups (p= 0.20 and 0.76, respectively). Testosterone deficiency in male CRF patients is not uncommon. Male patients with both T2DM and CRF have more common testosterone deficiency. In testosterone-sufficient patients, blood glucose regulation was better. Therefore, in these patients, it may be helpful not to neglect testosterone deficiency, which affects gonadal function, body metabolism, and cardiac and skeletal health.

Blood Profile of Rattus Nurvegicus Exposed to HgCl2 and Received Combination of IR Bagendit Rice Leaves-Infused Water and Young Coconut Water Budi Santosa
Medical Laboratory Technology Journal Vol. 8 No. 2 (2022): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Mercury chloride (HgCl2) is widely used in industries; in fact, this chemical substance is deadly for health and causes various health problems, such as liver damage, kidney damage, and hematopoietic disorders, especially in erythropoiesis. This study aims to determine the blood profile in Rattus nurvegicus exposed to HgCl2 by combining IR Bagendit rice leaves-infused water and young coconut water. This study employed an experimental method to examine the positive control group exposed to HgCl2 and the negative control group receiving a placebo. Meanwhile, the treatment groups received a combination of IR Bagendit rice leaves-infused water in stratified compositions and young coconut water. The hematologic profile was examined using a hematologic analyzer, while the reticulocyte count was examined using wet and dry methods. The mean value of each group was tested using ANOVA. This study has successively obtained average levels of Hb (C+ 12,0;C- 14,2;T1 12,5;T2 12,9;T3 12,8), the erythrocyte count (C+ 6,52;C- 7,80;T1 1,44;T2 1,07;T3 7,32), levels of Ht (C+ 35,5;C- 42,9;T1 37,8;T2 37,9;T3 38,2), MCV (C+ 73,7;C- 79,9;T1 80,4;T2 78,7;T3 79,6), MCH (C+ 27,3;C- 29,9;T1 29,5;T2 28,5;T3 29,1), and MCHC (C+ 31,1;C- 34,2;T1 31,9;T2 33,3;T3 32,1), and the reticulocyte count (C+ 2,0;C- 1,2;T1 1,3;T2 1,1;T3 1,3). The significant differences in the groups are found in levels of Hb, Ht, MCV, and MCHC (p-value 0,00; 0,00; 0,03; 0,01). This study concludes that combining IR Bagendit rice leaves-infused water and coconut water could prevent blood profile exposure to HgCl2. Suggestions for further research to increase the intervention time and measure oxygen levels.

Effect of Hematologic Values in Determining the Resolution of Subacute Thyroiditis and Long-Term Hypothyroidism Risk Savas Karatas; Burcu Hacıoğlu; Gökhan Kalaycı
Medical Laboratory Technology Journal Vol. 8 No. 2 (2022): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Subacute thyroiditis (SAT), which can be considered a disease with infectious signs and parameters, can affect humans and lead to permanent hypothyroidism. Haematological parameters play an essential role in SAT; these parameters begin to normalise after the thyrotoxic phase; however, there is still a gap in knowledge about how these parameters play a role in SAT prognosis. Therefore, we wanted to investigate haematological parameters and their impact on treatment response and recovery phase in patients with SAT. 51 SAT patients and 44 healthy controls were included in the study. ESR, CRP, NLR and MPV were recorded at the diagnosis and after resolution. The changes in these parameters were compared to determine the resolution phase. The ratio of permanent hypothyroidism after one year was studied, and its relevance with these parameters at the time of diagnosis was explored. 36 of 51 SAT patients were female in the study (70.5%). The mean age was 45.53 ± 11.46 years. In the acute phase, WBC, Neutrophil, platelet, ESR, N/L, and P/L were significantly higher (p<0.05). In the recovery phase, MPV and lymphocyte count were significantly higher (p: 0.002 and p=0.002). WBC, CRP, and ESR had the highest AUC values in ROC analysis (0.990, 0.959, 0.907, p<0.05). P/L and N/L ratios also had higher ratios for the acute phase (0.807, 0.98, p<0.05, respectively). Δ ESR, Δ CRP, Δ N/L, and Δ P/L had the highest AUC for the recovery phase (AUC= 0.990, 0.899, 0889,0.800, p<0.5, respectively). Permanent hypothyroidism after one year was 9/42 (21.4%). In addition to ESR and CRP, NLR, PLR and MPV were also successfully used to estimate SAT activity and response to treatment. Moreover, a decrease in NLR and PLR and an increase in MPV were associated with the treatment response and resolution phase. However, these parameters did not determine the long-term risk of hypothyroidism.

Semen Leucocytes Affect Sperm Quality of Infertility Patient Fitranto Arjadi; Vitasari Indriani; Wahyu Siswandari; Edy Priyanto; Mulyoto Pangestu
Medical Laboratory Technology Journal Vol. 8 No. 2 (2022): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

The association between the risk factors for male infertility including smoking, obesity, male age, and leukocyte count with sperm analysis, still shows mixed results. This study aims to determine the association between smoking, obesity, male age, and leukocytes count with sperm quality (sperm concentration, sperm motility, and sperm morphology) of infertility patients in Purwokerto. This study is an observational study with a cross-sectional design conducted in the medical records section of the Bunda Arif Hospital Purwokerto. The sample was taken by total sampling. The bivariate test of smoking and obesity behaviour variables with the results of sperm quality using the Chi-Square test and Fisher's exact test. Male age variables used the Kruskal-Wallis test and the Spearman correlation test. Variable leukocytes count using the Spearman test. The results showed no association between male age, obesity and smoking behaviour with sperm quality, sperm concentration, sperm motility, and sperm morphology (p>0.05). There is a significant association (p<0,05) between leukocyte semen count and spermatozoa concentration, sperm motility, and morphology of spermatozoa. It is concluded that there was a significant association between leukocyte count and sperm quality in infertility patients in Purwokerto. Research needs to be continued by examining the relationship between leukocytopenia and sperm DNA damage by looking at sperm DNA fragmentation.

The Platelet/Monocyte Ratio Before Treatment Predicts Prognosis in Solid Childhood Cancers Özlem Terzi; Cengiz Bayram; Ali Ayçiçek; Ezgi Uysalol; Cennet Nur Baş; Hüseyin Avni Solgun; Tuba Tahtakesen; Esra Arslantaş; Sibel Tekgündüz
Medical Laboratory Technology Journal Vol. 8 No. 2 (2022): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

The prognostic value of thrombocytosis and inflammatory biomarkers in adult cancer patients has been the subject of many studies. This study investigated the role of platelet count and other inflammatory biomarkers in the prognosis of solid childhood tumours. The data of 176 pediatric patients diagnosed with solid tumours were evaluated retrospectively; 150 patients still under follow-up were included in the study. We examined the relationship between platelet count, platelet/neutrophil ratio (PNR), platelet/lymphocyte ratio (PLR) and platelet/monocyte ratio (PMR) at the time of diagnosis on survival. The mean age of diagnosis was 7.91 ± 5.75 years, and 60.7% were male. The mean platelet count of the patients was 424.326 ± 167197.32. The median (range) follow-up was 13 (1-68) months. Relapse was seen in 18% of the patients, and 9.3% died. The relationship between PMR and overall survival (OS) and event-free survival times (EFS) was statistically significant (p=0.002 and p=0.016, respectively). However, no statistically significant association was found for PLR or PNR. Overall survival and EFS were significantly poorer in this cohort in patients with high pretreatment PMR.

Effects of Curcumin on Changes in Spermatogenetic Cells of Rats Treated with Cisplatin Şengül Şentürk; Mustafa Sandıkçı
Medical Laboratory Technology Journal Vol. 8 No. 2 (2022): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Cisplatin is an effective antineoplastic drug which is used to treat many types of cancer. The most common known side effect of this drug is infertility. Curcumin, also called turmeric, has antioxidant and antitumor activities. This study was designed to investigate the effects of curcumin on the changes in spermatogenetic cells caused by cisplatin, which is used as a chemotherapeutic drug especially in testicular cancers. For this purpose, randomly selected 36 Spraque Dawley rats were seperated to 4 groups that of control, cisplatin, curcumin and curcumin+cisplatin and each group was seperated to 3 subgroups, each of them have 3 rats, so total 12 subgroups were obtained. The testicular tissues obtained from rats were evaluated histochemically, immunohistochemically and ultrastructually. In the results of this study, it is observed that the rate of apoptosis increased significantly in the experimental groups given curcumin together with cisplatin injection, and the transition-1 protein which is involved in DNA packaging in the elongated spermatids belonging to stages of XII, XIII an XIV, immunopositivity is increased statistically in the cisplatin-administered experimental groups compared to control groups. Both light and electron microscopic findings showed that intense degeneration, vacuolization, germ cell loss and spermatogenetic arrest occurred especially in the group given curcumin together with cisplatin. The results of our study showed that cisplatin can cause problems in infertility through DNA packaging and dragging cells to apoptosis and necrosis. Physicians who use cisplatin for cancer treatment are advised not to use curcumin together with cisplatin because it exacerbates the side effects of the cancer drug.

Evaluation in Hematology and BCR-ABL Molecular Profiles in Patients with Chronic Myeloid Leukemia Undergoing Tyrosine Kinase Inhibitor Therapy Muhammad Ihza Lisan Shidqi; Budi Santosa; Muhamad Muslim; Haitami Haitami
Medical Laboratory Technology Journal Vol. 8 No. 2 (2022): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Chronic myeloid leukemia (CML) is a myeloproliferative malignancy due to the formation of the BCR-ABL fusion gene in chronic myeloid leukemia. This condition causes excessive cell proliferation, resulting in an increase in the number of leukocytes. Tyrosine Kinase Inhibitor (TKI) is a first-line therapy that helps reduce the percentage of the Breakpoint Cluster Region–Abelson (BCR-ABL) fusion gene in patients with chronic myeloid leukemia. This study was conducted to determine evaluation in the hematological profiles (hemoglobin levels, leukocyte counts, platelet counts) and molecular BCR-ABL in patients with chronic myeloid leukemia before and after 12 months of tyrosine kinase inhibitors therapy. This analytic observational study was administered using a cross-sectional design to in analyzing the medical records of CML patients who underwent TKI therapy at the Sub Specialist Polyclinic of Internal Medicine Hematology Oncology Ulin Banjarmasin Indonesia Regional Hospital from March 2021-April 2022. Statistical test was performed which analysis results showed that 12-month tyrosine kinase inhibitor therapy could increase the hemoglobin levels, decrease leukocyte counts, platelet counts as well as decreasing the percentage of BCR-ABL gene fusion in patients with chronic myeloid leukemia. In conclusion, evaluation of tyrosine kinase inhibitor therapy in patients with chronic myeloid leukemia obtained significant differences in the hematological profiles and the molecular BCR-ABL. Further researchers are recommended to compare the type of tyrosine kinase inhibitor therapy between Imatinib and Nilotinib on the hematological and molecular profiles of BCR-ABL in patients with chronic myeloid leukemia with a larger sample count.

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Ricko Dharmadi Utama

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Jl. H. Mistar Cokrokusumo No.1A, Kemuning, Kec. Banjarbaru Selatan, Kota Banjar Baru, Kalimantan Selatan 70714

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Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Ricko Dharmadi Utama

Contact Email apinaga53@gmail.com

Phone +6282256602225

Journal Mail Official editormltj@gmail.com

Editorial Address Jl. H. Mistar Cokrokusumo No.1A, Kemuning, Kec. Banjarbaru Selatan, Kota Banjar Baru, Kalimantan Selatan 70714

Location Kota banjarmasin, Kalimantan selatan INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Ricko Dharmadi Utama

Contact Email
apinaga53@gmail.com

Phone
+6282256602225

Journal Mail Official
editormltj@gmail.com

Editorial Address
Jl. H. Mistar Cokrokusumo No.1A, Kemuning, Kec. Banjarbaru Selatan, Kota Banjar Baru, Kalimantan Selatan 70714

Location
Kota banjarmasin,

Kalimantan selatan

INDONESIA