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BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan)
Published by Universitas Medan Area
ISSN : 25975269     EISSN : 2356458X     DOI : 10.31289
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Biolink focuses on the publication of the results of scientific research related to fields. This article is published in the internal and external academic community of the University of Medan Area (UMA) especially in the field of Biology. Biolink publishes scientific articles in the scope of biology that includes environmental biology, industrial biology and health biology. Published articles are the results of research articles, studies or critical and comprehensive scientific studies on important issues related to the field of biology.
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Articles 378 Documents
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GLANDULAR TRICHOME IN THE ASTERACEAE FAMILY Wina Dyah Puspita Sari; Cicik Suriani; Dina Handayani
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol 7, No 2 (2021): February 2021
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v7i2.3333

Abstract

The Asteraceae is a diverse plant species and widely distributed, especially in the tropics and subtropics, consisting of 1,600 - 1,700 genera which include 24,000 - 30,000  species. Asteraceae has characteristics of cup flowers and brackets that are not owned by other plants. The objective of this study was to determine the morphological structure of plants and escpecially the leaf glandular trichome in several species of Asteraceae. The research method used was a descriptive method, to describe and interpret the shape, structure and distribution of leaf trichome in the Asteraceae family. This study used eight species of the Asteraceae family, namely Elephantopus mollis, Bidens pilosa, Tithonia deversifolia, Tridax procumbens, Synedrella nodiflora, Eclipta prostrate, Sphagneticola trilobata and Ageratum conyzoides. The observation results of trichomes at 8 species by Scanning Electrone Microscope (SEM) was obtained varied forms of trichomes, both in shape and size. From the research, it was obtained that the forms of multicellular glandular trichome with various shapes, ranging in size from 50.6 µm - 831.9 µm.    
ANALYSIS OF DNA SEQUENCE ENCODING GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE (GAPDH) ON COCOR BEBEK (KALANCHOE x LAETIVIRENS) Putri Agustina; Dewi Indriyani Roslim
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol 8, No 1 (2021): August 2021
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v8i1.4964

Abstract

To understand the fundamental mechanisms on genes in plant cells can be performed by gene expression studies. The study needs reference genes that act as internal control to avoid expression data bias. Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) gene is one of reference genes that has been validated in plants. This study aims to analyze the DNA sequences encoding GAPDH in cocor bebek (Kalanchoe x laetivirens). Methods included total DNA extraction from fresh leaf, polymerase chain reaction (PCR), electrophoresis at 1% agarose and DNA sequence data analysis. The DNA sequence of K. x laetivirens GAPDH gene obtained were 1107 bp and predicted to consist of 5 exons and 5 introns. This sequence had about 68.96%-73.81% similarity to the DNA sequence of the GAPDH gene in several plant species. The amino acid sequence of K. x laetivirens GAPDH had 5 conserved regions and residues of cysteine (C) and histidine. The sequence obtained in this study was the first to be reported from the genus Kalanchoe. This sequence can be used for analysis of gene expression in K. x laetivirens and can be as reference for isolating the GAPDH gene for other plant species in the genus Kalanchoe.
THE POTENTIAL OF NGOKILO LEAVES EXTRACT (STACHYTARPHETA MUTABILIS. VAHL) AS A LOWERING OF BLOOD GLUCOSE LEVELS OF WHITE MICE (RATTUS NORVEGICUS) Mimatun Nasihah
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol 7, No 2 (2021): February 2021
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v7i2.3893

Abstract

Diabetes is a disease, in which the body cannot produce enough insulin so that there is excess sugar in the blood which becomes toxic to the body. Ngokilo plants are believed to be able to lower blood sugar levels because of the antioxidants and polyphenols contained therein.The objective of this study was to determine the potential of the ethanol extract of Ngokilo leaves (Stachytarpheta mutabilis, Vahl) as a lowering blood glucose levels in white mice(Rattus norvegicus). The tests were carried out by an experimental method using Swiss Webster male mice (Rattus norvegicus) aged 2-3 months with a body west of 30-40 grams as test animals. This study used four dosage ratios of ngokilo leaf extract, namely 1: 20: 40: 60 and control used aquadest. The results showed that the ethanol extract of Ngokilo leaves had an F value of 27.033> F Table 2.60. It indicates that the independent variable (the difference in the concentration of ethanol extract of Ngokilo leaves) was proven to have a significant effect on the dependent variable (blood sugar levels).
INCREASED RESISTANCE OF PENAEUS MONODON TO WHISPOVIRUS CAUSES WHITE SPOT SYNDROME THROUGH ANTIVIRUS GENE TRANSFER Chelvieana A. Christanti; Debora V. Widyanti; Yesica C. Rhenata; Floreta D. Icasari; David L Gultom; Sudarsono Siburian; Wahyu Irawati
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol 8, No 1 (2021): August 2021
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v8i1.4597

Abstract

The shrimp species Penaeus monodon is the main commodity of Indonesian aquaculture. One of the obstacles when cultivating tiger prawns is the attack of white spot disease (White Spot Syndrome Virus) which makes the quality and quantity of production decrease. One attempteffective for overcoming the white spot virus attack on shrimp, namely through the transfer of antiviral genes. Previous researchers carried out the transfer of antiviral genes to provide immunological effects so that they were resistant to pathogen attack and could prevent the death of tiger prawns. Destinationwriting article that is This study aims to determine the increase in resistance of tiger shrimp to Whispovirus that causes White Spot Syndrome through the transfer of antiviral genes. The type of research conducted is descriptive qualitative with a literature review method that discusses five focus studies, namely Whispovirus as a cause of White Spot Syndrome Virus (WSSV) disease in tiger prawns, tiger shrimp infected with White Spot Syndrome Virus, the use of PmAV antiviral genes in the treatment of Whispovirus , the mechanism of transfection of antiviral genes in the treatment of Whispovirus infection against tiger prawns, and changes in tiger shrimp resistance after insertion of antiviral genes. The conclusion obtained is that the addition of antiviral genes in tiger shrimp DNA can increase its resistance to Whispovirus.
ESTIMATION OF NUMBERS OF COLIFORM BACTERIAL AS WATER QUALITY INDICATOR IN KEPAHIANG DISTRICT RIVERS, BENGKULU PROVINCE Sipriyadi Sipriyadi; Risky Hadi Wibowo; Welly Darwis
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol 7, No 2 (2021): February 2021
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v7i2.3841

Abstract

Coliform is a group of microbes that are used as indicators of water quality. Water pollution is generally caused by pathogenic microbes from feces, household waste, and industrial activity waste. This study aimed to estimate the total number of coliform contamination in several rivers in Kepahiang Regency, namely Tebat Monok (TM), Sempiyang (SPY), Penanjung Panjang (PP), Embong Ijok (EI) Air Langkap(ALK), and Air Belimbing (ABB).  Total coliform and Fecal coliform tests were carried out using the Most Probable Number (MPN) method on Lactose Broth, Brillian Green Lactose Bile Broth and pour plates on Eosin Methylene Blue Agar media. Measurement of abiotic factors was on temperature and pH parameters. The test results of total coliform showed that 6 rivers contained total coliform under the Class II river water quality standards with a range of 1210/100 mL– 4310/100 mL and 2 rivers that were contaminated with Fecal coliform, TM and ALK, have the content of 1500/100 mL and 1700 / 100 mL. The results of the measurement of the abiotic factor, the river pH range was 7.4 - 8.2. The lowest temperature was 25oC in SPY river and the highest temperature was 26 oC on the TM, PP, EI, ALK, and ABB rivers.
ANTIBIOTIC SUSCEPTIBILITY TEST TOWARDS COCKROACH (PERIPLANETA AMERICANA) FOOT BACTERIA IN THE NUTRITION DEPARTMENT AREA OF PALU CITY HOSPITAL I Nengah Nengah Kundera; Yulia Windarsih; Abd. Rauf
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol 8, No 1 (2021): August 2021
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v8i1.4945

Abstract

Cockroaches (Periplaneta americana) are known to have potential as vectors of various pathogenic bacteria in various locations including the nutrition department area in the hospitals. Research related to the role of these animals as vectors of pathogenic bacteria has not been done. The provision of food in the hospital nutrition department has the potential for bacterial contamination from food, equipment, hospital staff, and the environment. Cockroaches can be a vector for spreading various nosocomial infectious diseases in hospitals. A descriptive method was used, and the data were obtained by bacterial isolation, Gram staining method, antibiotic sensitivity test, and bacterial identification using Microbact system software 2000. This study was carried out at the Biology Education Laboratory, Education and Teacher Training Faculty,  Tadulako University. Based on the results of the study, 6 species of bacterial isolates from cockroach feet   in the nutrition department area of Palu city hospital, namely E. coli, S. arizonae, Salmonella sp., K. ozaenae, S. simulans, and S. enterica. Antibiotic sensitivity test showed that four bacteria exhibited multi-drug resistance to antibiotics Amoxycillin, Chloramphenicol and Vancomycin. Meanwhile,S. enterica was still sensitive to the five tested antibiotics namely Amoxycillin, Chloramphenicol, Ciprofloxacin, Ofloxacin, and Vancomycin. serious policies and handling are needed to control new types of vectors that carry diseases in humans.. meanwhile,S. entericawas still sensitive to the five tested antibiotics.
DETERMINATION OF THE TROPICAL STATUS OF FLOATING NET CAGE WATER BASED ON THE DISTRIBUTION OF NITROGEN, PHOSPHORUS AND CHLOROPHYLL-A Peni Pujiastuti; Roesleini J Putri; Suseno Suseno
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol 7, No 2 (2021): February 2021
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v7i2.3902

Abstract

The dominant source of pollutants for floating net cages are fish feed and feces. They cause phosphorus and nitrogen in water increase, trigger eutrophication, marked by the appearance of algae. Algae are green plants, contain chlorophyll-a. The content of phosphorus, nitrogen and chlorophyll-a can be used to determine the tropical status of water. The objective of this research is to determine the tropical status of marine cage water. The research method was descriptive laboratory. Nitrogen content is measured as nitrite according to SNI 6989.9-2004, nitrate according to APHA Section 4500-NO3, ammonia according to SNI 19-1655-1989. Phosphorus analysis according to SNI 06-6989-31: 2005. Chlorophyll-a analysis used the Strickland & Parson method by spectrophotometry. Laboratory data were analyzed for tropical status based on nitrogen, phosphorus and chlorophyll-a content. The results showed that the tropical status of the KJA water of Gajah Mungkur Reservoir Wonogiri in the rainy season had eutrophic status, containing high levels of phosphorus and nitrogen elements. The eutrophic status indicated  that the water had been polluted by an enhancement of  nitrogen levels by 18.345 µg/L and phosphorus by 420.65 µg/L. These nitrogen and phosphorus pollutants increased the growth of chlorophyll-a by 12.70 µg/L.
VEGETATION COMPOSITION ON ECOLOGICAL FUNCTION IN MATARAM MERAH PARK, JAKARTA Nur Intan Mangunsong; Rini Fitri; Qurrotu Aini Besila
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol 7, No 2 (2021): February 2021
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v7i2.3883

Abstract

The existence of the city park is important for Jakarta as a metropolitan city with the current activity level of motor vehicles and air pollution greatly affects the quality of the city environment. The selection of vegetation in Mataram Merah park considered functional aspects and aesthetic values . The objective of this study were to determine the composition and type of vegetation landscape in Mataram Merah park and to determine the function of each type of landscape vegetation in Mataram Merah park. This study used a descriptive qualitative method with several stages, which is survey; identification of the name and type of vegetation; size identification; identification of the type of vegetation making up the composition (name of type and size that makes up the vegetation structure). The results showed that Mataram Merah park was generally planted with vegetation that functioned aesthetically rather than functionally. The function of landscape plants in Mataram Merah park were as aesthetics is 96.55%, as controlling view as much as 13.80%, vegetation function as physical barrier as much as 62.07%, plants as controlling erosion 51.72%, function of plants as controlling climate 13.79% and vegetation function for wildlife as much as 17.24%.
PROFILE OF PULMONARY AND EXTRA PULMONARY TUBERCULOSIS USE GENEXPER AT THE PIRNGADI HOSPITAL MEDAN Eva Sartika Daposang; Fenny Hasanah; Dewi Silaban
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol 8, No 1 (2021): August 2021
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v8i1.4638

Abstract

Mycobacterium tuberculosis as the bacteria that causes TB disease where the healing process takes a long time, using boredom and drug blockages in TB sufferers. This is one reason germs become resistant to several types of anti-tuberculosis drugs known as TB Multi Drug Resistance (MDR TB). The molecular rapid test (TCM) method using GenExper can quickly determine ATD resistance. This study aims to see the results of TCM examination using GenExper in patients with pulmonary tuberculosis and extra pulmonary tuberculosis at dr. Pirngadi Medan. This study used a cross-sectional method by taking laboratory data from the GenExper examination contained in the medical records of patients diagnosed with tuberculosis at Pirngadi Hospital, Medan. Samples obtained from patients using GenExper from January to December 2018 obtained 354 patients with 68.1% more TB incidence in male patients (241/354) than women by 31.9% (113/354). The patients most affected by TB were elderly people aged 56-65 years with a proportion of 23.7%. The largest population affected by TB is self-employed 30.5% and the highest level of education diagnosed is high school graduates 60.5%. Most patients suffered from pulmonary TB at 86.4%.
TEMPERATURE AND NITRATE CONCENTRATION EFFECT ON THE ABUNDANCE AND GROWTH RATE OF MELOSIRA SP. Nita Rukminasari; Sharifuddin Andy Omar; Muhammad Lukman
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol 7, No 2 (2021): February 2021
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v7i2.4054

Abstract

Microalgae are one of the natural resources that have high potential as a source of biofuels, one of them is the marine microalgae Melosira sp.. The objective of this study was to determine the effect of increasing temperature and nitrate concentration on the abundance, growth rate, biomass, organic C-content and free fatty acids of Melosira sp.. A Completely Randomized Design was used for this experiment with four temperature treatments and three nitrate concentrations treatments  in microalgae test planting media. The test microalgae were cultured for 21 days, while the abundance of Melosira sp..was carried out every 3rd day, while for other parameters measurements were made at the end of the experiment. The results showed that the increasing in temperature and nitrate concentration in culture media significantly affected the abundance of Melosira sp.. Meanwhile, the results of statistical analysis showed that the increase in temperature and concentration of culture media did not have a significant effect on biomass except at 30°C treatment, the dry weight of Melosira sp. was only significantly different between the nitrate concentrations of 0.0 g/L and 0.4 g/L. For the content of C-organic and free fatty acids generally showed a significant difference between treating temperature and nitrate concentration in the culture media.

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