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All Journal Jurnal Ilmu Pertanian Indonesia AL KAUNIYAH Bulletin of Chemical Reaction Engineering & Catalysis Elkawnie JURNAL ENGGANO BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan) Jurnal Pengabdian Pada Masyarakat Martabe : Jurnal Pengabdian Kepada Masyarakat BIOEDUSAINS: Jurnal Pendidikan Biologi dan Sains Jurnal Mangifera Edu Dharma Raflesia : Jurnal Ilmiah Pengembangan dan Penerapan IPTEKS Jurnal Pengabdian kepada Masyarakat Nusantara SN-Biosper Jurnal Pengabdian Pada Masyarakat Bulletin of Chemical Reaction Engineering & Catalysis
Risky Hadi Wibowo
Universitas Bengkulu
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Keanekaragaman Makrofungi Basidiomycota di Desa Barumanis dan Kawasan Hutan Sekitarnya, Kecamatan Bermani Ulu, Kabupaten Rejang Lebong Vero Neka Emelda; Welly Darwis; Risky Hadi Wibowo; Sipriyadi Sipriyadi; R R. Sri Astuti
Seminar Nasional Biologi, Saintek, dan Pembelajarannya (SN-Biosper) Seminar Nasional Biologi, Saintek, dan Pembelajarannya III Tahun 2021
Publisher : Seminar Nasional Biologi, Saintek, dan Pembelajarannya (SN-Biosper)

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Makrofungi merupakan fungi yang dapat dilihat dengan mata secara langsung, sebagian makrofungi hidup ditanah dan sebagian hidup sebagai safrofit pada tanaman dan ada juga yang hidup bersimbiosis dengan akar tanaman. Fungi Basidiomycota adalah fungi yang memiliki basidiokarp atau tubuh buah yang besar yang dapat dilihat dengan mata secara langsung  dan memiliki ciri-ciri diantarannya mempunyai basidium dan koneksi penjepit. Desa Barumanis terletak di Kecamatan Bermani Ulu Kabupaten Rejang Lebong,  Provinsi Bengkulu. Kawasan di sekitar hutan Desa Barumanis memiliki suhu yang dingin sehingga berpotensi memiliki keanekaragaman makrofungi khususnya filum  Basidiomycota.  Karakteristik kawasan lingkungan Desa Barumani terletak di kawasan kaki Bukit Daun dimana sebagian besar wilayahnya merupakan  perkebunan dan pertanian. Tujuan penelitian ini yaitu untuk mendapatkan jenis makrofungi filum Basidiomycota yang tumbuh di Desa Barumanis dan kawasan hutan sekitarnya Kecamatan Bermani Ulu, Kabupaten Rejang Lebong. Metode yang digunakan dalam penelitian ini yaitu metode jelajah (Cruise Method) yaitu dengan menjelajahi Desa Barumanis dan kawasan hutan sekitarnya, Kecamatan Bermani Ulu, Kabupaten Rejang Lebong. kemudian data yang diperoleh diolah secara analisis deskripsi kualitatif. Dari hasil penelitian yang telah dilakukan ditemukan spesies makrofungi yaitu Homidicutis marginata, Lycoperdon pyorforme, Fomitopsis rosea, Tramella fuciformis, Ganoderma lobatum, Auricuaria americana, Mycena fumosa, Baespoora myosura, Baespoora myosura, Galerina autumnalis, Phelinus gilvus, Hyplohoma acutum, Laccaria amethystina, Cantharellus cinnabarinus, Coprinus variegatus, Mycena leptothepalla, Agrocybe cylindracea, Stereum ostrea, Pleorotus sp. Makrofungi yang diperoleh dari penelitian ini diharapkan menjadi informasi keanekaragaman jenis makrofungi dari filum Basidiomycota di Desa Barumanis dan kawasan hutan sekitarnya Kecamatan Bermani Ulu, Kabupaten Rejang Lebong dan melengkapi data makrofungi yang terdapat di Provinsi Bengkulu.
Aktivitas Antibakteri dan Analisis Fitokimia Ekstrak Metanol dari Daun Paku Sarang Burung (Asplenium nidus) Risky Hadi Wibowo; Redo Setiawan; Welly Darwis; Sipriyadi Sipriyadi; Rochmah Supriati; Alfredi Anis Fadhila Ginting Sinisuka
Jurnal Ilmu Pertanian Indonesia Vol. 27 No. 3 (2022): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.27.2.295

Abstract

Infectious diseases caused by microbes are increasing; the growth of microbes can be inhibited by using antibiotics. Continuous and inappropriate use of antibiotics causes microbes to become resistant. The resistance can be reduced by finding new sources of antibiotics, one of which is from the bird nest fern (Asplenium nidus). This study aimed to determine the potency and concentration of A. Nidus extract in inhibiting the growth of Staphylococcus aureus ATCC 6538, Escherichia coli ATCC 8739, Pseudomonas aeruginosa ATCC 15442, and Bacillus subtilis ATCC 19659. The extract was prepared using the maceration method with methanol as the solvent. Phytochemical tests include alkaloids, flavonoids, steroids, terpenoids, saponins, phenolics, tannins, and quinones. The minimum inhibitory concentration test was carried out on 12 treatments with three replications; the antimicrobial effectiveness test was carried out on seven treatments with five replications using the disc diffusion method. Phytochemical test results showed A. Nidus contains flavonoids, tannins, saponins, and phenolics. A. nidus extract has the potential to inhibit the growth of the testing pathogenic bacteria, with the most effective concentration on P. aeruginosa of 45% with an inhibitory zone was about 14,16 mm, on E. Coli started of 55% with an inhibitory zone was about 13,68 mm, on B. Subtilis of 65% with an inhibitory zone was about 14,80 mm and S. aureus was 75% with an inhibitory zone of 11,96 mm. Keywords: antibiotics, Asplenium nidus, Infectious disease
Soil Chitinolytic Bacteria from Jambi Province to Produce Antifungal of Plant Pathogens Risky Hadi Wibowo; Sipriyadi Sipriyadi; Nisa Rachmania Mubarik; Iman Rusmana
Mangifera Edu Vol 5 No 1 (2020): Jurnal Mangifera Edu
Publisher : Universitas Wiralodra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31943/mangiferaedu.v5i1.95

Abstract

Chitinolytic bacteria are bacteria that have chitinolytic activity, which is able to hydrolysis the composition of chitin which composes many fungal cell walls. Chitinolytic bacteria are currently more widely used because of their ability as a biological control agent to the pathogenic fungi especially in horticultural and plantation crops. This research was conducted with the aim of obtaining isolates of chitinolytic bacteria that were able to inhibit the growth of plant pathogenic fungi in Vitro on chitin agar media. Fusarium oxysporum, Sclerotium rolfsii, and Rhizoctonia solanii are used in the inhibition test of chitinolytic bacteria. Bacteria were isolated and screened from the soil of Bukit Dua Belas National Park and Oil Palm Plantations in Jambi using 0.3% chitin agar media. The results showed that two of 10 bacterial isolates were able to produce inhibition zones to the growth of hyphae of pathogenic fungi on potato dextrose agar (PDA) media. TB04-13 isolate was able to produce the largest inhibition in F. oxysporum and R. solanii about 42% and 42.05% respectively, while TB04-15 isolate produced the biggest inhibition in S. Rolfsii ranged to 25.50%. Based on the chitinolytic index (CI) values, isolates TB04-13 and TB04-15 produced CI values ​​of 1.60 and 0.63, respectively. The morphological characteristics and Gram staining of both TB04-13 and TB04-15 chitinolytic isolates are included in rod-shaped and Gram-positive bacteria. Both of these isolates can be used as antifungal-producing candidates for plant pathogenic fungi in Indonesia.
ESTIMATION OF NUMBERS OF COLIFORM BACTERIAL AS WATER QUALITY INDICATOR IN KEPAHIANG DISTRICT RIVERS, BENGKULU PROVINCE Sipriyadi Sipriyadi; Risky Hadi Wibowo; Welly Darwis
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol 7, No 2 (2021): February 2021
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v7i2.3841

Abstract

Coliform is a group of microbes that are used as indicators of water quality. Water pollution is generally caused by pathogenic microbes from feces, household waste, and industrial activity waste. This study aimed to estimate the total number of coliform contamination in several rivers in Kepahiang Regency, namely Tebat Monok (TM), Sempiyang (SPY), Penanjung Panjang (PP), Embong Ijok (EI) Air Langkap(ALK), and Air Belimbing (ABB).  Total coliform and Fecal coliform tests were carried out using the Most Probable Number (MPN) method on Lactose Broth, Brillian Green Lactose Bile Broth and pour plates on Eosin Methylene Blue Agar media. Measurement of abiotic factors was on temperature and pH parameters. The test results of total coliform showed that 6 rivers contained total coliform under the Class II river water quality standards with a range of 1210/100 mL– 4310/100 mL and 2 rivers that were contaminated with Fecal coliform, TM and ALK, have the content of 1500/100 mL and 1700 / 100 mL. The results of the measurement of the abiotic factor, the river pH range was 7.4 - 8.2. The lowest temperature was 25oC in SPY river and the highest temperature was 26 oC on the TM, PP, EI, ALK, and ABB rivers.
Isolation and Screening of Soil Chitinolytic Actinobacteria as the Anti-Fungal Producer of Plant Pathogens Risky Hadi Wibowo; Sipriyadi Sipriyadi; Nisa Rachmania Mubarik; Iman Rusmana; Maggy Thenawidjaya Suhartono
Elkawnie: Journal of Islamic Science and Technology Vol 6, No 2 (2020)
Publisher : Universitas Islam Negeri Ar-Raniry Banda Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/ekw.v6i2.7400

Abstract

Abstract: Chitinolytic actinobacteria are currently more widely used because of their ability as the biological control agents to the pathogenic fungi, especially in horticultural and plantation crops. This research was conducted to obtain isolates of chitinolytic soil actinobacteria from the rhizosphere of the rubber plant (Hevea brasiliensis) area in IPB University. Antifungal activities from these actinobacteria hopely able to inhibit the growth of plant pathogenic fungi in Vitro on chitin agar media. Fusarium oxysporum and Sclerotium rolfsii are used in the inhibition test of chitinolytic actinobacteria. The results successfully obtained 16 isolates of actinobacteria were grew on  Humic Acid Vitamin (HV) agar. It showed that six of 16 actinobacteria isolates were able to produce inhibition zones to the growth of hyphae of pathogenic fungi on potato dextrose agar (PDA). KK-15 and KK-07 isolates were able to produce the largest inhibition percentages in F. oxysporum and S. rolfsii. Based on the chitinolytic index (CI) values, KK-15 and KK-07 isolates produced CI values of 1.25 and 1.5, respectively. The morphological characteristics and Gram staining of both KK-15 and KK-07 isolates are closely relative with Streptomyces sp. Abstrak: Bakteri kitinolitik saat ini banyak diteliti karena kemampuannya sebagai agens pengendali hayati jamur patogen khususnya pada tanaman hortikultura dan perkebunan. Penelitian ini dilakukan untuk mendapatkan isolat-isolat bakteri kitinolitik asal tanah dari bagian perakaran tanaman karet (Hevea brasiliensis) di daerah perkebunan karet Institut Pertanian Bogor (IPB). Aktivitas antifungi dari bakteri ini diharapkan  mampu menghambat pertumbuhan jamur patogen tanaman secara in Vitro pada media agar-agar kitin. Fusarium oxysporum dan Sclerotium rolfsii digunakan dalam uji hambat aktinobakteri kitinolitik. Hasil penelitian memperoleh 16 isolat aktinobakteri berhasil diisolasi dengan menggunakan media agar-agar Humic Acid Vitamin (HV), dan dari 16 isolat tersebut, enam isolat mampu menghasilkan zona hambat terhadap pertumbuhan hifa dari kedua jamur patogen pada media Potato Dextrose Agar (PDA) secara in Vitro. Isolat KK-15 dan KK-07 mampu menghasilkan diameter hambatan terbesar pada jamur F. oxysporum dan S. rolfsii. Berdasarkan nilai index kitinolitik (IK), isolat KK-15 dan KK-07 menghasilkan nilai IK sebesar 1,25 dan 1,5 secara berurutan. Karakteristik morfologi dan pewarnaan Gram dari kedua isolat yaitu KK-15 dan KK-07 memiliki kedekatan dengan kelompok Streptomyces spp.
Reduction of 4-nitrophenol Mediated by Silver Nanoparticles Synthesized using Aqueous Leaf Extract of Peronema canescens Salprima Yudha S; Aswin Falahudin; Risky Hadi Wibowo; John Hendri; Dennie Oktrin Wicaksono
Bulletin of Chemical Reaction Engineering & Catalysis 2021: BCREC Volume 16 Issue 2 Year 2021 (June 2021)
Publisher : Department of Chemical Engineering - Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.9767/bcrec.16.2.10426.253-259

Abstract

In this study, we developed an alternative of 4-nitrophenol reduction mediated by silver nanoparticles (AgNPs) which was synthesized using aqueous extract of the Peronema canescens leaf through an eco-friendly approach. The reducing 4-nitrophenol to 4-aminophenol mediated by AgNPS in the presence of sodium borohydride as a hydrogen source proceeded rapidly at room temperature without any additional treatments. The AgNPS synthesis was simple and was carried out under mild conditions. Ultraviolet–visible spectroscopy was performed to examine the properties of the obtained AgNPs, which displayed an absorption peak at 431 nm. A transmission electron microscopy analysis revealed that the AgNPs were spherical in shape and had an average particle size of 19 nm as determined by particle size analysis. Copyright © 2021 by Authors, Published by BCREC Group. This is an open access article under the CC BY-SA License (https://creativecommons.org/licenses/by-sa/4.0). 
POTENSI ISOLAT Bacillus sp. ENG-4 YANG BERASOSIASI DENGAN SPONS Aplysina sp. PENGHASIL SENYAWA ANTIMIKROB ASAL PULAU ENGGANO Risky Hadi Wibowo; Sipriyadi Sipriyadi; Welly Darwis; Santi Nurul Kamilah; Hizkia Puspa Pertiwi; Reza Pertiwi
JURNAL ENGGANO Vol 5, No 1
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (400.81 KB) | DOI: 10.31186/jenggano.5.1.1-10

Abstract

Pulau Enggano merupakan salah satu pulau terluar di Indonesia yang memiliki keragaman biota yang cukup tinggi, salah satunya adalah hewan spons. Spons adalah sekelompok hewan multiseluler, memiliki ciri tubuh berpori, dan organisme invertebrata berguna dalam memproduksi senyawa bioaktif. Bakteri yang terkait dengan spons dapat menjadi sumber alternatif senyawa bioaktif baru, terutama antimikrob. Penelitian ini bertujuan untuk mengetahui potensi bakteri yang terkait dengan Aplysina sp. asal Pulau Enggano sebagai penghasil senyawa antimikrob melawan mikrob patogen. Total 263 isolat berhasil diisolasi pada media agar-agar Sea water Complete (SWC). Isolat bakteri potensial selanjutnya diidentifikasi dengan pengamatan morfologi, pewarnaan Gram, serta dilanjutkan dengan uji biokimia. Hasil uji aktivitas antimikrob dari 16 isolat terpilih melalui uji antagonis, supernatan dan pelet terhadap mikrob patogen Escherichia coli, Staphylococcus aureus, Pseudomonas aeroginosa dan Candida albicans menunjukkan bahwa isolat ENG-4 memiliki kemampuanmenghambat pada dua mikrob pathogen yaitu E. coli, S. aureus. Isolat ENG-4 termasuk ke dalam bakteri gram positif dan merujuk pada genus Bacillus berdasarkan uji biokimia.POTENTIAL OF ISOLATE Bacillus sp. ENG-4 ASSOCIATED WITH MARINE SPONGE Aplysina sp. PRODUCING ANTIMICROBIAL COMPOUNDS FROM ENGGANO ISLAND. Enggano Island is one of the outer islands in Indonesia that has a high diversity of marine biota, one of them are sponges. Sponge is a group of multicellular animals, has a characteristic porous body, and invertebrate organisms are useful in producing bioactive compounds. Bacteria associated with sponges can be an alternative source of new bioactive compounds, especially antimicrobial. This study aims to determine the potential of bacteria associated with Aplysina sp. from Enggano island as a producer of antimicrobial compounds against pathogenic microbes. Total 263 isolates were successfully isolated in Sea water Complete (SWC) agar media. Potential isolates were further identified morphologically, Gram staining, and also biochemical tests. The results of the antimicrobial activity test of 16 selected isolates through antagonist, supernatant and pellet tests on pathogenic microbes of Escherichia coli, Staphylococcus aureus, Pseudomonas aeroginosa and Candida albicans showed that the ENG-4 isolate had the ability to inhibit two spesies of pathogens which are E. coli and S. aureus. ENG-4 isolate belong to gram-positive bacteria and refer to the genus Bacillus based on biochemical tests.
PENYULUHAN DAN PEMBUATAN PAPER SOAP (SABUN KERTAS) BAGI MASYARAKAT DI KELURAHAN PEMATANG GUBERNUR KOTA BENGKULU UNTUK MENINGKATKAN KESADARAN MENCUCI TANGAN SEBAGAI LANGKAH AWAL PENCEGAHAN WABAH COVID-19 Risky Hadi Wibowo; Sipriyadi Sipriyadi; Reza Pertiwi; Thoriqul Hidayah; Della Indah Medani; Gustina Dwi Wulandari; Nadya Rosianti
Martabe : Jurnal Pengabdian Kepada Masyarakat Vol 4, No 1 (2021): Martabe : Jurnal Pengabdian Kepada Masyarakat
Publisher : Universitas Muhammadiyah Tapanuli Selatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31604/jpm.v4i1.317-324

Abstract

Hand is the main transmission pathogen entry into the body which is the part of the body that is most often in direct contact with other limbs and surrounding objects. The importance of maintaining hand hygiene by always washing hands with soap is the first step in preventing transmission of COVID-19. However, during a pandemic like this, it will be difficult to find soap available everywhere, and tends to be hard to carry all the time. Therefore, this service aims to invite the people of Pematang Gubernur, Bengkulu City, to use paper soap because it is easier to use its use and flexible to carry anywhere besides being an effort to prevent the COVID-19 outbreak, it can also add to new information and skills for residents in making attractive soaps. The feedback response given by the community service implementation team to the community service participants resulted in a positive response to this activity, who initially did not know about making and use of paper soap and became aware of this. 
Aktivitas Antibakteri Nanopartikel Perak Hasil Green Synthesis Menggunakan Ektrak Kulit Buah Kemuning (Murraya Paniculata (L) Jack) Doni Notriawan; Gustria Ernis; Risky Hadi Wibowo; Reza Pertiwi; Tessa Rianti Malau
BIOEDUSAINS: Jurnal Pendidikan Biologi dan Sains Vol 3 No 2 (2020): BIOEDUSAINS:Jurnal Pendidikan Biologi dan Sains
Publisher : Institut Penelitian Matematika, Komputer, Keperawatan, Pendidikan dan Ekonomi (IPM2KPE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (413.997 KB) | DOI: 10.31539/bioedusains.v3i2.1850

Abstract

Antibacterial activity using silver nanoparticles synthesized using Murraya Paniculata (L) rinds extract has been carried out. This study aims to determine the antibacterial activity of green synthesized silver nanoparticles using Murraya Paniculata (L) rinds extract. Silver nanoparticles were synthesized by reacting AgNO3 with water extract of the Murraya Paniculata (L) rinds. Green synthesized silver nanoparticles were tested for antibacterial activity using Escherichia coli ATCC 8739. Silver nanoparticles synthesized using Murraya Paniculata (L) rinds extract were characterized using a UV-Vis spectrophotometer to confirm the formation of silver nanoparticles. The UV-Vis spectrophotometer results showed the formation of silver nanoparticles which was indicated by an absorption around 400-450 nm. The antibacterial activity test results of silver nanoparticles showed a strong inhibitory activity. Keywords: Green Synthesis, Silver Nanoparticles, Antibacterial, Echerichia Coli
Aplikasi Akuaponik Sayur Organik-Ikan Lele dalam Ember (Asoileledamber) di Kota Bengkulu Risky Hadi Wibowo; Sipriyadi Sipriyadi; Nanang Sugianto; Stella Reformanda Sembiring; Margareth Hutasoit; Yuni Kristina Serlyani; Thoriqul Hidayah
Jurnal Pengabdian Pada Masyarakat Vol 5 No 3 (2020)
Publisher : Universitas Mathla'ul Anwar Banten

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30653/002.202053.561

Abstract

AQUAPONIC APPLICATION OF ORGANIC VEGETABLES-CATFISH IN BUCKETS (ASOILELEDAMBER) IN BENGKULU CITY. Bengkulu City is the capital of Bengkulu Province with the highest population in Bengkulu Province so it requires the availability of high food consumption as well. Required foodstuffs such as vegetables, meat, and fish, not all are available from Bengkulu City, so it is not uncommon the price on the market becomes more expensive. To fulfill the needs of foodstuffs, some people of Bengkulu City make effort to cultivate vegetables and maintain fish in ponds, but this method is also difficult to do because of the limited land area. Based on the description, it is necessary to apply the Appropriate Technology (TTG) for the people of Bengkulu City to fulfill family food security, one of which is the application of aquaponic technology: Asoileledamber (Organic Vegetable-Catfish Aquaponics in Buckets). This community service activity aims to provide information and training to the group of service partners in Bengkulu City about the benefits of Asoileledamber. Community service activities include Asoileledamber counseling activities, the provision of guidebooks, provision of tools and materials for cultivation such as fish seedlings, vegetable seeds and pellet feed. The results perceived by the service partners were harvesting Catfish from the second month of maintenance to the fourth month of maintenance. Organic vegetables are harvested starting from 2 weeks after planting time (SMT) until the 4th month of maintenance with an average harvest of 1-2 bunches per bucket.
Co-Authors Aldi Trianda Alfredi Anis Fadhila Ginting Sinisuka Angga Martias Aswin Falahudin Aulia Adriansyah Avidlyandi, Avidlyandi Charles Banon Choirul Muslim Della Indah Medani Della Indah Medani Dennie Oktrin Wicaksono Dhea Amelia Sari Dian Fita Lestari Elsi Silvia Fatimatuzzahra Gustina Dwi Wulandari Gustina Dwi Wulandari Gustria Ernis Helmiyetti Helmiyetti Hizkia Puspa Pertiwi Iman Rusmana John Hendri Khafit Wiradimafan Lisna Hamni Maggy Thenawidjaya Suhartono Margareth Hutasoit Morina Adfa Nadya Rosianti Nadya Rosianti Nanang Sugianto NISA RACHMANIA MUBARIK Notriawan, Doni R R. Sri Astuti Redo Setiawan Reza Pertiwi Reza Pertiwi Reza Pertiwi Reza Pertiwi Reza Wahyuni Rochmah Supriati Sal Prima Yudha SALPRIMA YUDHA S Santi Nurul Kamilah Sipriyadi Stella Reformanda Sembiring Tessa Rianti Malau Thoriqul Hidayah Thoriqul Hidayah Triawan, Deni Agus Vero Neka Emelda Vestidhia Yunisya Atmaja Welly Darwis Welly Darwis Welly Darwis Welly Darwis Welly Darwis Yuni Kristina Serlyani