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Articles 482 Documents
Nine Additional Cultivated Species of Asteraceae from Java Irsyam, Arifin Surya Dwipa; Irwanto, Rina Ratnasih
Jurnal Biodjati Vol 4 No 2 (2019): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v4i2.4815

Abstract

Asteraceae is one of the largest families in the Angiosperm. It comprises of 24,000‒30,000 species which are grouped into 1,600‒1,700 genera and distributed in a cosmopolitan range. Based on Backer and Bakhuizen van den Brink’s 1965 works, they identified 227 species of Asteraceae in Java. Many of them are introduced species, both cultivated or naturalized. However, the cultivated species have not been recorded. The aim of this study is to provide information about new records of cultivated Asteraceae in Java. The observations were carried out in Bandung, Bogor, and Sumedang, during January to February 2019. Nine additional species were discovered; species belonging to genera Caputia (two species), Curio (two species), Kleinia (one species), Gymnanthemum (one species), Senecio (two species), and Tarlmounia (one species). These species were grouped into two tribes: Vernonieae and Senecioneae. This study indicates that information on Asteraceae of Java need to be updated.
Potential and Challenges on Amphibians and Reptiles Research in West Java Cahyadi, Ganjar; Arifin, Umilaela
Jurnal Biodjati Vol 4 No 2 (2019): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v4i2.4820

Abstract

 West Java Province has the largest population amongst others in Java, and therefore land conversion rate in the region is increasing. Approximately 40% of forest areas in West Java has been converted between 1990–2015. As a consequence, the number of bi­odiversity in the region is decreasing, including amphibians and rep­tiles. These groups play an important role in the food chain of an ecosystem, and are very sensitive to environmental changes. How­ever, comprehensive research on amphibian and reptile species in West Java is suboptimal. Visual Encounter Survey has performed in seven districts in West Java for one month and has recorded 26 amphibian species and 27 reptile species. These species were in­cluding Javan endemic species (for example: Fejervarya iskandari, Huia masonii, Limnonectes microdiscus, Megophrys montana, and Microhyla achatina), introduced species (Calotes versicolor), and species with a new distribution record (Leptophryne borbonica and Kalophrynus minusculus). In addition, cryptic species (Genus Lep­tophryne and Cyrtodactylus), which are interesting for further stud­ies, were observed. This study has demonstrated that the potential of a comprehensive study of amphibian and reptile species in West Java is great. Apart from this, conserving the biodiversity in the region also challenging due to the high rate in land conversion
Odonata Diversity at Sumber Clangap and Sumber Mangli Puncu Village Sub District of Puncu District of Kediri Abdillah, Muhammad Muhibbuddin; Prakarsa, Tatag Bagus Putra; Tyastirin, Esti
Jurnal Biodjati Vol 4 No 2 (2019): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v4i2.4823

Abstract

 Sumber Clangap and Sumber Mangli are geographically located at the Mount Kelud steeps. Administratively located at Puncu Village, Puncu Sub-district and District of Kediri. They provided hab-itat for the flora than fauna especially Odonata that never been stud-ied before. We aimed to study Odonata diversity at Sumber Clangap and Sumber Mangli area. The method used in this study was natural snapshot experiment that conducted by Odonata monitoring. Micro-climate parameter including air temperature and humidity were not-ed. Odonata activity and behavior noted for analysis. Collected data were analyzed using Shannon-Wiener heterogeneity index. The results showed that there were 17 species from the whole location. There was Euphaea variegata, Vestalis luctuosa, Rhinocypha anisoptera, Peric-nemis stictica, Pseudagrion pruinosum, Coeliccia membranipes, Gy-nacantha subinterrupta, Idionyx montana, Paragomphus reinwardtii, Heliogomphus drescheri, Neurothemis fluctuans, Orthetrum glau-cum, Orthetrum pruinosum, Orthetrum sabina, Pantala flavescens, Trithemis festiva and Zygonyx ida. Based on the Shannon-Wiener heterogeneity index the value, Sumber Clangap had heterogeneity in-dex higher (H’=1,97) than Sumber Mangli (H’=1,39). Sumber Man-gli has a Java endemic species Paragomphus reinwardtii and Rhi-nocypha anisoptera that is spread only at Sumatera and East Java. 
Actinomycetes from the Soil of Chilli Plantation in Yogyakarta Showing an Antagonism to Fusarium oxysporum FU3 Audinah, Liya; Ilmi, Miftahul
Jurnal Biodjati Vol 4 No 2 (2019): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v4i2.5237

Abstract

 Application of biocontrol agent is an environmental-ly-friendly method to overcome the Fusarium wilt in chilli due to Fusarium oxysporum. This research aimed to isolate actinomycetes antagonist to F. oxysporum FU3 as biocontrol agent from the soil of chilli plantation in Kulon Progo, Yogyakarta. The actinomycetes were isolated using SCA medium and followed by screening for anti-F. oxysporum FU3 on PDA. The filtrate from isolates which showing the highest inhibition zone was produced and tested against strain FU3. The best isolate was then characterized. There are four iso-lates of actinomycetes showing antagonistic activity against F. ox-ysporum (AK4, AK5, AK7, and AK9). The highest antagonistic ac-tivity was shown by AK5 filtrate with inhibitions on PDA and PDB are 41.71 ± 3.5 % and 81.77 ± 5.1 %, respectively. Further obser-vation showed that filtrate of AK5 caused abnormality and lysis on FU3 hyphae. From the results, we concluded that AK5 is a poten-tial biocontrol agent against F. oxysporum. Further studies to opti-mize the production and formulize the bio-fungicide are suggested 
Effectiveness of Lactic Acid Bacteria to Improve Cyprinus carpio Fingerlings Resistance Against Rosidah, Rosidah; Mulyani, Yuniar; Lili, Walim; Rosyidah, Khasanatur
Jurnal Biodjati Vol 5 No 1 (2020): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v5i1.5260

Abstract

Common carp (Cyprinus carpio) cultivation is often hampered by a disease attack, one of them is the attack of Edwarsiella tarda. Lactic acid bacteria (LAB) can be used as an alternative to prevent diseases in fish by increasing the body's resistance. This research aimed to determine the most effective isolates of LAB that increase of the resistance of carp fingerlings to the attack of E. tarda bacteria and see which isolates can produce the highest survival. The LAB isolates used were the result of isolation from the gut of carp. This study used a Completely Randomized Design (CRD) consisting of four treatments with three replications. The fish were immersed with different LAB isolates, CcB7, CcB8, and CcB15 in the same density of 108 cells / mm3. Immersion was carried out for 30 minutes with a frequency of seven days. While during the research, two immersions were carried out before the challenge test against E. tarda bacteria. The parameters observed were the number of leukocytes, hematocrit, erythrocyte, differential leukocytes, survival rate, and clinical symptoms that appeared. The results showed that all LAB isolates used in this study could increase the body resistance of carp against the attacks of E. tarda bacteria. The LAB CcB7 isolate was the most effective for enhancing the body resistance of carp fish withthe highest increase level of  leukocyte, erythrocyte, and hematocrit were 18 ± 0.057,7 ± 0,077, and 0.26± 7.31% respectively. After being challenged with E. tarda bacteria producing mild clinical symptoms, the highest increase is in monocyte and neutrophil cells was 20 and 62% respectively, the highest reduction in lymphocytes was – 9%  and the highest survival rate was 80%. 
Molecular Identification of Bacillus Derived from the Common Carp Intestine and Their Antibiotics Resistance Mulyani, Yuniar; Syakirotin, Karimah; Haetami, Kiki; Mulyani, Yeni; Liviawaty, Evi; Agung, Mochamad Untung Kurnia
Jurnal Biodjati Vol 5 No 2 (2020): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v5i2.5263

Abstract

The use of bacteria as a biological control agent in aquaculture is such new hope overcoming the negative impacts of the impropriate use of antibiotics. Bacillus is widely known as a potential probiotic for aquaculture, especially which are derived from aquatic biota. Due to a concern that the role of Bacillus as a probiotic agent will be disrupted by residues of antibiotics in the water, this research aimed to identify Bacillus species from common carp intestine based on the molecular method and investigate their resistance to some com-mercial antibiotics that widely used in aquaculture. Chloramphenicol, ampicillin, and metronidazole were used to determine the Bacillus resistance to antibiotics using the Kirby Bauer method. The resistance assay was carried out with a bacterial density of 108 CFU/mL. The results showed that Bacillus isolates derived from common carp  intestine were closely related and resistant to antibiotics. Isolates that resistant to the three types of antibiotics were Cc.1.9 (CgN9) and Cc.2.18 (CgM18).
Amphibians Diversity in Sulap Hill and Watervang Dam, Lubuklinggau Rozi, Zico Fakhrur; Samitra, Dian
Jurnal Biodjati Vol 5 No 1 (2020): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v5i1.5340

Abstract

Sulap Hill and Watervang Dam are located in the central of the city of Lubuklinggau, South Sumatra Province, which has a variety of organisms, including amphibians. This study aimed to determine the diversity of amphibian species in the area of  Sulap Hill and Watervang Dam Lubuklinggau City. This research was conducted from June to August 2018. Data collection was an exploration method-combined visual encounter survey (VES). The samples obtained were identified at the Biology Education Laboratory, STKIP PGRI Lubuklinggau. Data analysis included species composition, diversity index, and Evenness index, and conservation status. The results of the study found 5 families, consist of 11 species: Duttaphyrnus melanotictus, Ingerophrynus divergens, Phrynoidis asper, Fejervarya cancrivora, Fejervarya limnocharis, Limnonectes kuhlii, Limnonectes macrodon, Limnonectes paramacrodon, Polypedates leucomystax, Hylarana erythraea, Ichthyophis sumatranus. The results of the data analysis showed that the amphibian diversity index in the Sulap hill (1.55) and Watervang dam (1.84) was categorized as low. The Evenness index in Sulap Hill (0.86) and Watervang Dam (0.88) was categorized as a high uniformity and stable community. Based on data from the International Union for Conservation of Nature and Natural Resources (IUCN) Red List, 8 species have the status of Least Concern, 1 species with Vulnerable status, 1 species with Near-threatened status and 1 species with data deficient status.
Immunoinformatics Study on Early 4 Protein of Human Papillomavirus Type 16 for Cervical Cancer Vaccine Peptide Candidate Suryani, Yani; Taupiqurrohman, Opik; Yusuf, Muhammad; Subroto, Toto; Nuswantara, Sukma
Jurnal Biodjati Vol 4 No 2 (2019): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v4i2.5414

Abstract

 The aims of this study were to carry out testing of the early 4 protein of type 16 HPV through immunoinformatics meth-ods in an effort to get the peptide vaccine candidate for cervical cancer. The software used are IEDB-AR, CABSdock and Accelrys Discovery Study 4.5. Based on the analysis that sequence of ami-no acid lysine, leucine, leucine, glycine, serine, threonine, tryp-tophan, proline and threonine (KLLGSTWPT) and the sequence of amino acid tyrosine, tyrosine, valine, leucine, histidine, leucine, cysteine, leucine, alanine, alanine, threonine, lysine, tyrosine, pro-line and leucine (YYVLHLCLAATKYPL) are peptide vaccine can-didate for cervical cancer from the early 4 protein of HPV type 16 
Antioxidant, Antibacterial Activity and GC-MS Analysis of Extract of Giant Forest Ant Dinomyrmex gigas (Latreille, 1802) Evana, Evana -; Pratiwi, Pratiwi; Fathoni, Ahmad; Efendi, Oscar; Agusta, Andria
Jurnal Biodjati Vol 4 No 2 (2019): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v4i2.5440

Abstract

 Giant forest ant Dinomyrmex gigas is one of the largest ants species in the world, native to the rain forests of Southeast Asia. It is known that ants have glands that produce chemical compounds that inhibit the growth of microbes. Therefore, it is necessary to deter-mine the antioxidant and antibacterial activities as well as identify the chemical compounds of D. gigas extract. D. gigas was extracted successively with n-hexane, ethanol and methanol. The antioxidant activity was evaluated by determination of the half-maximal inhibi-tory concentration (IC50) values while the antibacterial activities of the extracts were determined by measuring the minimum inhibitory concentration (MIC). The results exhibited that the IC50 values of n-hexane, ethanolic and methanolic extracts were 336.18±0.0984, 89.16±0.0219 and 90.72±0.0894 μg/mL respectively. The ethanolic extract exhibited the highest AAI value (0.34) followed by metha-nolic extract (0.33) and n-hexane extract (0.09). Based on AAI val-ues, the extracts were classified as moderate antioxidants. The best MIC values were 625 μg/mL for both ethanolic and methanolic ex-tracts against S. aureus, while MIC values of all extracts against E. coli were >625 μg/mL. Based on MIC values, all of the extracts presented weak activity against both S. aureus and E.coli. The GC-MS analysis showed that there are up to 30 compounds construct-ed of the ethanolic extract. Three major compounds are ethyl oleate  (29.78%), n-hexadecanoic acid (17.54%) and oleic acid (10.65%). 
Growth of Slipper Orchid Paphiopedilum javanicum (Reinw. ex Lindl.) Pfitzer during Acclimatization Stage Hendriyani, Ema; Warseno, Tri; Oktavia, Gebby Agnessya Esa
Jurnal Biodjati Vol 4 No 2 (2019): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v4i2.5665

Abstract

 Paphiopedilum javanicum (Reinw. ex Lindl.) Pfitzer known as slipper orchid is an endemic terrestrial orchid from Indonesia. This orchid is threatened with extinction and listed under CITES Appendix I. In-vitro propagation of P. javanicum has been done in Bali Botanic Garden (BBG) for last six years but acclimatization stage has not given satisfied result. The purpose of this study was to know the ef-fect of liquid fertilizer on vegetative growth of P. javanicum planlet during acclimatization stage. Beyonic StarTmik liquid fertilizer ap-plied by five different doses considered as treatment of 0, 10, 20, 30 and 40 mL. They were 10 planlets for each treatment considered as replication. In this research we observe vegetative growth of P. ja-vanicum by quantitative and qualitative parameter. Quantitative data were analysis by ANOVA. Result showed, high percentage of planlet survival was observed of 98% and liquid fertilizer dose for 30 mL gave the optimal vegetative growth of P. javanicum planlet with aver-age height of 2.2 cm and leaves number of 13 pieces. There were no differences on qualitative parameter in all treatment that all leaves color was dark green and leaves tessellation appearance was obviousÂ