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INDONESIA
Jurnal Biodjati
Published by Universitas Islam Negeri Sunan Gunung Djati Bandung
ISSN : 25481606     EISSN : 25414208     DOI : 10.15575/biodjati
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences
Arjuna Subject : -
Articles 482 Documents
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Pengaruh Ekstrak Etanol Daun Sambiloto Terhadap Jumlah dan Motilitas Spermatozoa Mencit Jantan Susilo, Susilo; Akbar, Budhi; Pratinaningsih, Ika
Jurnal Biodjati Vol 3 No 2 (2018): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v3i2.3505

Abstract

Tanaman sambiloto (Andrographis paniculata Ness.) di Indonesia dikenal sebagai salah satu tanaman yang berkhasiat sebagai obat. Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak etanol daun sambiloto terhadap jumlah dan motilitas spermaozoa mencit (Mus musculus L.) jantan galur DDY. Sebanyak 24 ekor mencit jantan dengan berat antara 100-125 g yang dibagi menjadi empat kelompok perlakuan, diberikan ekstrak etanol secara oral selama 15 hari. Kelompok mencit pada perlakuan satu (P1) diberikan ekstrak etanol daun sambiloto 84 mg/kg bb. Kelompok mencit pada perlakuan dua (P2) dan tiga (P3) masing-masing diberikan ekstrak etanol daun sambiloto 140 mg/kg bb dan 196 mg/kg bb. Sedangkan kelompok kontrol (P0) tidak diberi perlakuan. Hasil penelitian menunjukkan bahwa pada kelompok perlakuan P3 (196 mg/kg bb) menunjukkan jumlah spermatozoa terbanyak yaitu 7,33 juta/ml, mengalami penurunan sebesar 78,86% terhadap kontrol.  Kandungan senyawa andrografolid dan flavonoid pada ekstrak sambiloto diduga kuat menjadi penyebab penurunan jumlah dan motilitas spermatozoa mencit. Hasil penelitian ini memberikan informasi bahwa pemberian ekstrak etanol daun sambiloto berpengaruh terhadap jumlah dan motilitas spermatozoa mencit jantan.
Effect of Water Temperature to Survival and Development of Larvae of Two Local Aedes Aegypti Strains Putra, Ramadhani Eka; Trinuroni, Gunadi
Jurnal Biodjati Vol 4 No 1 (2019): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v4i1.3843

Abstract

  Global warming has been reported in last decades. Chang-es in average Earth’s temperature may affect the physiology of many insect species, especially the ones which act as a human disease vec-tor, like Aedes aegypti. In this study, the effect of increasing water temperature on physiological components related to development period, sex ratio, and body size of two distinctively strains, VCRU (Vector Control Research Unit) and Pangandaran. Thirty larvae of each strain kept inside distilled water with the temperature of 25°C, 27°C, 30°C, 33°C, 35°C, 40°C which replicated three times. Observa-tions were conducted until all larvae metamorphed into adults or all larvae dead. Development rate and time were analyzed by frequency dependent mean. The result showed that the optimum temperature for larvae development of VCRU strain was 27-30°C with a survival rate of 84% while it was 30°C for Pangandaran strain, with the surviv-al rate of 83%. Larvae of both strains showed 100% mortality rate when kept inside a container with water temperature exceed 33°C. Both strains showed the highest and the lowest male:female ratio at similar water temperature which were 33°C and 30°C, respec-tivelly. Highest and lowest ratio of VCRU was 1.25 and 0.6, respec-tively, while it was 1.4 and 0.6 for Pangandaran. In general, larvae lived in increasing water temperature showed reducing wing width. 
Molecular Characterization Reveals Genetic Differences Between Wild and Captive Populations of Mandiangin Giant Gourami (Osphronemus goramy) Nuryanto, Agus; Susilo, Maria Bramasti; Amurwanto, Adi
Jurnal Biodjati Vol 4 No 1 (2019): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v4i1.3942

Abstract

  Captive population of Mandiangin giant gourami (Osphronemus goramy Lac.) is a new strain developed from wild pop-ulations in Riam Kanan Dam. No much is known about the genetic constituent of this strain compared to the parental population. This re-search was done to obtain information whether genetic alteration has occurred in captive population compared to their wild parental pop-ulation. Wild population was caught from Riam Kanan Dam, while captive population was collected from Balai Perikanan Budidaya Air Tawar (BPBAT) “Freshwater Aquaculture Centre” Mandiangin, South Kalimantan. The captive population of Batanghari strain from BPBAT Sungai Gelam, Jambi was also analyzed. Molecular assess-ment was done using CO1 PCR-RFLP which was developed by digest-ing the amplicon of the CO1 gene from all populations using two re-striction enzymes, namely HindIII and TaqI and resulted CO1-HindIII and CO1-TaqI RFLP markers. All tested populations showed uniform patterns of those CO1-RFLP markers but with different fragment sizes among populations. This means all resulted RFLP markers were mono-morphic in each populations but polymorphic among giant gourami populations. In other words, no genetic diversity within population but substantial genetic differences was observed between wild and cap-tive Mandiangin populations. This proved that genetic alteration has occurred in Mandiangin captive populations compared to the wild pa-rental giant gourami population. In conclusion, captive and wild pop-ulations of Mandiangin giant gourami were genetically different. This result is vital as scientific base for future development of inland fish-eries, especially for breeding strategy of Mandiangin giant gourami. 
The Fish Fauna in Lakitan River, Musi Rawas Regency, South Sumatra Samitra, Dian; Rozi, Zico Fakhrur
Jurnal Biodjati Vol 4 No 1 (2019): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v4i1.4097

Abstract

  Fish diversity in the Lakitan River is not well recorded, even though the data is important to add information about the diversity of freshwater fish in Indonesia. This study aims to study fish composition and ecological index in the Lakitan River. The study was conducted in the Lakitan River, Musi Rawas Regency, South Sumatra Province. The method used was the survey method. The direct sampling was done at 5 stations, the numbering of stations followed the direction of the river from upstream to downstream. The data at each station are tabulated based on species, family and order. Data analysis in-cludes a diversity index, evenness index, dominance index, and sim-ilarity index. The fish caught during the study were 418 individuals, which were identified into 20 species and 11 families. Barbonymus gonionotus is the most captured species in the Lakitan River. Cypri-nidae is the most captured family (9 species). The highest diversity index was found at station 5, with the number of fish collected was 16 species. The evenness index at 5 stations showed the equivalent species distribution and stable communities. The domination index at 5 stations were at, low category. The similarity index between sta-tions in Lakitan River which ranging from 0.65 – 0.97. These results indicate that fish diversity in the Lakitan River medium biodiversity. 
The Role of Endothelial Microparticle in Coronary Heart Disease as The Complications of Diabetes Mellitus Elfi, Eka Fithra; Ilhami, Yose Ramda; Darwin, Eryati
Jurnal Biodjati Vol 4 No 1 (2019): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v4i1.4164

Abstract

  Coronary heart disease (CHD) is caused by obstruction of coronary blood flow due to endothelial dysfunction triggered by various genetic and non-genetic risk factors such as hyperlipidemia, hypertension, hyperglycemia and obesity. Endothelial cell activation due to hyperglycaemia in diabetes mellitus induces production of pro-inflammatory factors that damage the cell membrane triggering the formation of membrane particles called microparticles. Endothe-lial microparticles contain proteins including endothelial nitric oxide synthase (eNOS) which plays a role in the production of nitric oxide (NO). To determine the role of microparticles in the occurrence of coro-nary heart disease in diabetes mellitus due to endothelial dysfunction, a study was conducted by comparing the levels of eNOS and NO in DM patients who had CHD with DM patients who had no CHD. Blood samples from 20 DM patients who had CHD and 20 DM patients who had no CHD of the outpatients in Cardiology Department and Inter-nal Medicine department of regional public hospital were included in this study. All patients were fulfilled inclusion and exclusion criteria and diagnosed by the appropriate specialist. The eNOS and NO lev-els were measured using the ELISA method. The results of this study show that eNOS levels in the group of DM patients who had CHD (21,292±12,415 ng/ml) were significantly lower (p <0.05) than those in the group of DM patients who had no CHD (29,721±11,952 ng/ml). Nitric oxide levels in DM patients who had CHD (0,053±0,021 nmol/ μl) were not statistically different to the levels in DM patients who had no CHD (0,047±0,032 nmol/μl). From the results of this study we concluded that endothelial microparticle protein eNOS plays a role in the occurrence of CHD due to the complications of diabetes mellitus 
Isolation and Detection of Bacteriocin-Like Inhibitory Substances-Producing Bacteria from Fermented Mare’s Milk Sumbawa Mulyawati, Alifia Issabella; Ardyati, Tri; Jatmiko, Yoga Dwi
Jurnal Biodjati Vol 4 No 1 (2019): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v4i1.4194

Abstract

  Bacteriocin-like inhibitory substances (BLIS) produced by bacteria is a promising future food preservative agent. This study aimed to obtain bacterial strains that can produce broad-spectrum antibacterial agents and identify the best BLIS producer species based on 16S rDNA sequences. The bacterial strains were isolated from fer-mented mare’s milk using MRS and M17 agar medium. The isolates then were initially screened based on its antibacterial activity of crude cells against Staphylococcus aureus ATCC 6538. The selected strains were cultured and harvested for its cell-free supernatant (CFS). The pH of CFS was adjusted to 6.5 then used for antibacterial activity as-says against ten pathogenic bacteria. Also, the proteinaceous nature of BLIS compound was confirmed by testing with proteinase K. The gDNA of selected isolates was extracted and the 16S rDNA was am-plified using the polymerase chain reaction method then sequenced. The 16S rDNA sequences of the selected strains were used to identify the species using BLAST nucleotides from NCBI then the phylogenetic trees were constructed. 32 isolates was obtained, but only three iso-lates (BC9, SB7, and DC12) were selected as a result of antibacterial screening for further assays. The neutralized-CFS (N-CFS) of these isolates exhibited broad-spectrum antibacterial activity. The N-CFS could be assumed as BLIS. The isolate of BC9 was identified as Ba-cillus amyloliquefaciens strain BC9 that has 99.99 % similarity with B. amyloliquefaciens KC-1, SB7 was Lactobacillus plantarum strain SB7 that has 99.99 % similarity with Lb. plantarum JMC 1149T, and DC12 was Lactobacillus rhamnosus strain DC12 that has 100 % sim-ilarity with Lb. rhamnosus DSM 20021T. Thus, the BLIS produced by those strains is potential for future food and beverages preservations. 
Growth Rate and Capsaicin Level of Curly Red Chili (Capsicum annum L.) on Biofertilizer and Biogas Sludge Application Siswanti, Dwi Umi; Lestari, Melinda Fajar
Jurnal Biodjati Vol 4 No 1 (2019): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v4i1.4216

Abstract

Curly red chili (Capsicum annum L.) is widely cultivated us-ing inorganic fertilizers which causes high production costs. Chili is valued because of the level of spiciness. The level of spiciness of chili is determined by the level of capsaicin which is affected by nutrients in the growing media. The nutrient of the planting media can be fulfilled by degrading the growing media by microbes in organic fertilizer. One type of organic fertilizer containing degrading microbes is biofertiliz-er. Biofertilizer and biogas sludge is one of the organic fertilizers con-taining microbes and organic materials that are able to support nutri-ent levels of the planting medium. This study aimed to analyze growth parameters include chlorophyll content and measure capsaicin levels in curly red chili. Which given biofertilizer and biogas sludge in vari-ous dosage and determine the optimum dose of the fertilizer. The study was conducted at an agricultural demonstration plot in Wukirsari Vil-lage, Cangkringan, Sleman. The study design used RCBD (Randomized Complete Block Design), the data were analyzed by ANOVA (Analysis of Variance followed by Duncan’s Multiple Distance Test (DMRT) at the 95% confidence level. The highest growth parameters and capsa-icin content were obtained on curly red chili plants which were given biogas sludge 36 mL + biofertilizer 10 L / ha. The application of bi-ofertilizer and biogas sludge in various concentrations given has not been able to increase the average total chlorophyll content of curly red chili plants. Thus, it can be concluded that the most appropriate dose of curly red chili is 36 mL biogas sludge + 10 L bio fertilizer/ha
Phytochemical Screening and Antibacterial Activity of Acorus calamus L. Extracts Muchtaromah, Bayyinatul; Hayati, Alfiah; Agustina, Erna
Jurnal Biodjati Vol 4 No 1 (2019): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v4i1.4235

Abstract

  Staphylococcus aureus and Escherichia coli are among the most common species of gram-positive and gram-negative bacteria, which cause vaginitis, in infertile women. The Calamus rhizome (Acorus calamus L.) is an Indonesian plant that has antibacterial properties that can be used to treat vaginitis and increase fertility. The aim of this study was to determine the phytochemical and antibacterial activity of the calamus rhizoma in polar, semi-polar and non-polar solvents in the growth of S. aureus and E. coli. The antibacterial activity test was in the form of inhibitory test using the Kirby-Bauer, Minimum Inhibi-tion Concentration (MIC) and Minimum Bactericidal Concentration (MBC) by microdilution method with multilevel dilution (concentra-tions 50; 25; 12.5; 6.25; 3.13; 1.56; 0.78; and 0.39%). The screening results showed that ethanol and n-hexane extract contained alkaloids and triterpenoids, while chloroform extract was only triterpenoid. Chloroform extract produced the largest inhibition zone diameter of S. aureus and E. coli (7.26 and 3.28 mm), followed by ethanol extract (5.90 and 3.07 mm) and n-hexane extract (5.33 and 2.95 mm). The concentrations of 0.39 and 0.78% were the values of MIC and MBC for all three extracts, indicating that the extract of the calamus rhizome with several solvents in this study had the same antibacterial activity. 
Vegetation Analysis of the Secondary Forest Area of ​​Mount Merapi National Park Haryadi, Haryadi; Sunarto, Sunarto; Sugiyarto, Sugiyarto
Jurnal Biodjati Vol 4 No 1 (2019): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v4i1.4239

Abstract

 Mount Merapi National Park (MMNP) is a natural con-servation area with the main function of preserving the diversity of plants, animals and their ecosystems. In 2010, Mount Merapi suffered a large eruption which caused damage to the forest ecosystem inside. One of the locations that suffered severe damage was at Cangkrin-gan Resort. After the eruption, the affected vegetation began to grow again. The purpose of this study is to monitor and determine the di-versity of secondary forest plants in the MMNP area. The method used was a method of path and use a point-intercept transect as the sam-pling unit. The results showed that after the eruption, the Acacia de-curens dominated at each level starting from sapling, poles and trees. Based on the results of the study showed that the eruption of Mount Merapi that occurred in 2010 resulted in a change in vegetation struc-ture in the area with the Shannon-Weiner diversity index which is still low, amounting to 1.86 for seedling, 1.32 for the sapling and 0.22 for poles. At the tree level 100% is still dominated by Acacia decurens.  
The Capability of Consortium Phosphate Solubilizing Bacteria and IAA Producing Fungi on Promoting Elephant Grass Growth Imaningsih, Witiyasti; Kadarsah, Anang; Rusmannurrachmad, Raden Dwi Thriwantho
Jurnal Biodjati Vol 4 No 1 (2019): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v4i1.4284

Abstract

 Phosphorus compound in the critical land is relatively low that caused phosphorus is bonded to metals. The isolate BRC-AP and BRC-AK are a phosphate solubilizing bacteria (PSB) indigenous critical land that has the ability to dissolve phosphate. The used of phosphate solubilizing bacteria are able to improve plant growth on critical lands. The aimed of this study was to measure the growth rate of shoot length, root length and biomass of root, and leaf cuttings elephant grass (Pennisetum purpureum cv. King Grass) which inocu-lated with the consortium of phosphate solubilizing bacteria and IAA producing mold. Phosphate dissolving activity is determined by the formation of clear zone in solid Pikovskaya medium, synergism assay between bacteria and fungi observed on SDA medium then proceed testing the ability of bacteria and fungi consortium to promote plant growth. The result showed that the highest of phosphate solubility in-dex was found in isolate BRC-AP (27), addition of the combination of isolate BRC-AP, BRC-AK and Humicola sp. R.Dn have significant effect on shoot growth in the first week and leaf dry weight. The high-est length shoot of cutting elephant grass was found in treatment with the consortium of phosphate solubilizing bacteria and IAA producing mold (BRC-AP + BRC-AK + KP) (58.66 cm). The highest of leaf dry weight was found in treatment with BRC-AP + BRC-AK + KP (5.65 gr). The addition of consortium phosphate solubilizing bacteria and IAA producing fungi from South Kalimantan critical land proved can improve plant growth especially length shoot and leaf dry weight.

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