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INDONESIA
BIOEDUKASI
Published by Universitas Jember
ISSN : 16933931     EISSN : 25800094     DOI : -
Core Subject : Science, Education, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Library & Information Science
Arjuna Subject : -
Articles 7 Documents
 1 
Search results for , issue "Vol 18 No 1 (2020)" : 7 Documents clear
The Toxicity of Srikaya Seed Granules (Annona squamosa L.) with Different Heating Temperatures Against the Larva of Aedes aegypti L. Rima Gloria Purwanto; Dwi Wahyuni; Joko Waluyo
BIOEDUKASI Vol 18 No 1 (2020)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v18i1.15683

Abstract

Abstract: Aedes aegypti L. is a mosquito carrying dengue virus that causes dengue fever, especially in Southeast Asia which is a tropical rain forest region which is a habitat for mosquito growth. Aedes aegypti L. mosquito control is carried out by chemical means of fogging and using abate but this control actually causes Aedes aegypti L. mosquitoes to become resistant or immune to chemical drugs so that a new breakthrough is created by making bioinsecticides biologically to eradicate the Aedes aegypti L. mosquito. with characteristics that are environmentally friendly and do not kill non-target animals and cause the Aedes aegypti L. mosquito not to become resistant. To be able to realize this desire so that the sugar cane granules extract of Annona squamosa L. containing active compounds in the form of annonain and squamosin so that they are toxic to Aedes aegypti L. mosquito larvae. Further research is to test the heating temperature level of the granules of Annona squamosa L. extract temperature of 40 ° C and 60 ° C which is more deadly of Aedes aegypti L. mosquito larvae. This research method uses a completely randomized design with four repetitions. The temperature of 60 ° C uses concentrations of 1 ppm, 6 ppm, 12 ppm, 18 ppm, 24 ppm and 30 ppm. As for the temperature of 40 ° C using concentrations of 1 ppm, 7 ppm, 14 ppm, 21 ppm, 28 ppm, and 35 ppm, each temperature compared with aquadest and abate. Data analyzed using probit analysis to determine the LC50, then followed by a statistical test paired sample T-test with SPSS to find out a significant difference between heating temperatures of 40 ° C to 60 ° C. These results then show that a higher temperature of 60 ° C has a higher level of toxicity compared to a lower temperature of 40 ° C.
Ordo Anura in Jember University Naufal Fa'iq Hilmi; Jekti Prihatin; Vendi Eko Susilo
BIOEDUKASI Vol 18 No 1 (2020)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v18i1.16721

Abstract

Some types of Anura have a narrow range of environmental parameters, so they can’t survive in environments where natural conditions change dramatically. Therefore, Anura has the potential to be a good environmental bioindicator. The purpose of this research is to identify the types of Anura found at University of Jember. This research is included in the survey research using direct observation method VES (Visual Encounter Survey). Observations were made of adult members of the Anura Order. Data taken includes species characteristics, abiotic factors such as temperature and humidity, and activity when found. The results showed at Jember University there were 4 species from 3 family members of the Anura order, namely Duttaphrynus melanostictus Schneider, 1799 (Bufonidae), Polypedates leucomystax Gravenhorst, 1829 (Rhacophoridae), Fejervarya limnocharis Gravenhorst, 1829 (Dicroglossidae), and Occidozyga sumatrana Peters, 1877 (Dicroglossidae). The species most commonly found is Duttaphrynus melanostictus which is found in almost every observation location. Environmental factors that support the existence of Anura members in that location are temperature and humidity, and environmental conditions.
Identification of Bacteria on Seblak Food around University of Jember Based on Microbiological Criteria of Processed Food Rizka Maulidya Cahyani; Joko Waluyo; Mochammad Iqbal
BIOEDUKASI Vol 18 No 1 (2020)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v18i1.14835

Abstract

The quality of food that is good in bacteriological, chemical and physical must always be maintained in order to avoid diseases or health problems. Healthy and safe food is an important factor to improve the standard of public health. Seblak is a ready-to-eat Indonesian food which until now has never been carried out research about what kinds of bacteria in it. Seblak is a food made from raw crackers which is then deliberately soaked using hot water to have a chewy texture. This study aims to determine what types of bacteria are contained in seblak, through the process of isolation and identification in the macroscopic, microscopic and biochemical way. This study used 5 samples, which was repeated 5 times for each sample. Bacteria were isolated from the sample using spread plate techniques and observed by growing colonies on the plate. Each different colonies was observed microscopically through gram staining and endospore staining. To strengthen the data, biochemical tests were also carried out, biochemical tests that have been done in this study were the oxidase test, catalase test, and indole test. The results of the study showed that the bacteria that were found from the samples are in the genus of Bacillus sp. because they show the morphological characteristics of the colonies that form concentric circles, meanwhile, microscopic observations show morphological characteristics of cells in the form of gram-negative bacilli and have the endospores.
Microbiological Quality of Coriander in Malang Traditional Market Anindya Nirmala Permata; Utami Sri Hastuti; Sulisetijono Sulisetijono
BIOEDUKASI Vol 18 No 1 (2020)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v18i1.17042

Abstract

Coriander is commonly used by people as a food flavour. Actually, people does not separate the intact and damaged coriander for food processing. The aim of this study is to examine the microbiological quality of the intact and damaged coriander based on the Total Plate Count (TPC) of mold colonies and identification of the mold contaminants. Identification of contaminants mold based on the colony and microscopic character description. Then, the fungi characters refers to the identification key book for fungi identification. The research results is: 1) the intact coriander TPC is 1.6 x 103 colonies/g and the damaged coriander TPC 1.4 x 107 colonies/g. 2) There are 10 species of molds contaminant isolated from the intact coriander and damaged coriander.
Effect of Variations in Sugar Addition to the Quality of Bamboo Shoot Syrup (Dendrocalamus asper) Soleman Sayuna; James Ngginak; Merpiseldin Nitsae
BIOEDUKASI Vol 18 No 1 (2020)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v18i1.15445

Abstract

Bambu betung (Dendrocalamus asper) merupakan tumbuhan berumpun yang memiliki fase tumbuh melalui tunas (rebung). Rebung memiliki kandungan fosfor, vitamin A, vitamin C, serat mineral dan protein yang baik untuk tubuh. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh variasi penambahan gula terhadap kualitas sirup rebung betung. Metode yang digunakan dalam penelitian ini adalah metode eksperimen dengan rancangan acak lengkap (RAL) untuk uji sifat organoleptik. Variasi penambahan gula yang digunakan dalam penelitian ini adalah 50%, 55%, 60% dan 65%. Hasil uji vitamin C secara berturut - turut adalah 2,112%, 3,256%, 4,136%, dan 5,016%. Hasil uji protein secara berturut – turut untuk setiap perlakuan adalah 0,25%, 0,15%, 0,24% dan 0,17%. Hasil uji kadar air menunjukkan 0,073%, 0,063%, 0,056% dan 0,056%. Serta hasil uji oganoleptik (kekentalan, rasa dan kesukaan) menunjukkan perlakuan terbaik terdapat pada kosentrasi gula 65% dengan nilai 3.8000, 3.4667 dan 3.6000. Hasil penelitian ini menunjukkan terdapat pengaruh variasi gula terhadap vitamin C, kadar air dan organoleptik, akan tetapi tidak memiliki pengaruh terhadap kadar protein. Perlu adanya penelitian lanjutan untuk mengetahui nilai gizi lemak, Karbohidrat, Vitamin A, vitamin B1 (Thiamin), Vitamin B2 (Ribovlavin), Fe, K, Ca, P, Na dan uji mikrooganisme.
Crabs in Bandealit Estuary Resort of Meru Betiri National Park Galuh Paramita; Wachju Subchan; Vendi Eko Susilo
BIOEDUKASI Vol 18 No 1 (2020)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v18i1.16776

Abstract

Crabs are macrobenthos with characters that are attached to or resting on a base or live on basic sediments. The role of crabs in the ecosystem includes converting nutrients and enhancing mineralization, increasing the distribution of oxygen in the soil, helping carbon life cycles, and providing natural food for various types of aquatic biota. This research was conducted in the estuary area of ​​the Meru Betiri National Park (TNMB) Resort Bandealit. Identification results from 37 individual crabs at the Estuary Resort Bandealit Meru Betiri National Park (TNMB) obtained 5 species from 2 families (Portunidae and Sesarmidae). In the family Portunidae, there are 3 types, namely Scylla tranquebarica, Scylla paramamosain, and Scylla olivacea. Whereas in the family Sesarmidae found 2 types, namely Parasesarma pictum and Parasesarma cognatum. Keywords: Crab, Bandealit Resort, Identification
In-Silico Analysis for cryI Gene Amplification from Bacillus thuringiensis Febriana Dwi Wahyuni; Henny Saraswati; Kartika Sari Dewi
BIOEDUKASI Vol 18 No 1 (2020)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v18i1.16430

Abstract

Abstract Bacillus thuringiensis is one type of bacteria that has been used as a microbiological control agent for pests and a vector of plant disease. The presence of Cry proteins inside the B. thuringiensis can be acted as a specific insect repellent that only toxic to certain insects. The CryI protein is toxic to Lepidoptera insects which can attack various types of plants. Polymerase Chain Reaction (PCR) is a common method that can be used to amplify the gene encoding CryI proteins from B. thuringiensis. This research aimed to design a good primer candidate for cryI gene amplification from B. thuringiensis. In silico analysis for designing cryI primer was carried out using some software, such as BLAST for searching cryI gene sequence, Bioedit for sequences alignment, and DINAmelt for analyzing dimer structure of primers. Ten primer candidates were successfully obtained based on the result of the primer3 software. A pair of primer was selected to amplify the cryI gene, with forward primer 5’- CGGTGAATGCCCTGTTTACT -3’ and reverse primer 5’-CGGTCTGGTTGCCTATTGAT -3’. Amplification of the cryI gene by PCR method using selected primer resulting in a PCR product with a length of approximately 200 bp.

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