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Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology

squalen
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Published by Pusat Litbang Daya Saing Produk dan Bioteknologi Kelautan dan Perikanan

ISSN : 20895690 EISSN : 24069272 DOI : -

Core Subject : Science, Agriculture, Social,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology

Squalen publishes original and innovative research to provide readers with the latest research, knowledge, emerging technologies, postharvest, processing and preservation, food safety and environment, biotechnology and bio-discovery of marine and fisheries. The key focus of the research should be on marine and fishery and the manuscript should include a fundamental discussion of the research findings and their significance. Manuscripts that simply report data without providing a detailed interpretation of the results are unlikely to be accepted for publication in the journal.

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Articles 363 Documents

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Optimization of Processing Conditions of Alkali Treated Cottonii (ATC) from Sap-free Eucheuma cottonii Fateha, Fateha; Wibowo, Singgih; Santoso, Joko; Agusman, Agusman; Uju, Uju
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 14, No 2 (2019): August 2019
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Alkali treated cottonii (ATC) is a derivative product of Eucheuma cottoniithat is treated with alkali. This study used raw material of sap-free seaweed for ATC processing. Sap-free seaweed is a seaweed which thallus has been extracted with liquid known as sap. The use of sap-free seaweed as an ATC product is constrained by its low quality, so an effort to improve its quality is needed. Therefore the obejcetive of this Research was to get the optimal conditions of ATC processing. Experimental data were designed and analyzed using Response Surface MethodologyCentral Composite Design (RSM-CCD) using Design Expert 10.0.7Â® program. The optimization of ATC processing involved three components that were considered influential, namely KOH concentration, temperature, and processing time. Recommendation for optimal conditions issued by RSM-CCD on ATC processing from sap-free seawee d were 6 % KOH concentration at 75 oC for 120 minutes (93.1% desirability). The results of the response analysis showed a yield of 39.47% and a gel strength of 595.32 g/cm2. As a research control, the recommendation of RSMCCD used in ATC processing from nonsap-free seaweed was obtained yield of 36.81% and gel strength of 574.44 g/cm 2. ATC from sap-free seaweed has higher yield and gel strength than that from nonsap-free seaweed. Thismight be due to the sap-free seaweed was obtained using the proper sap extraction process and ATC processingunder optimal conditions, so that the quality of seaweed was better maintained.

Potency of Catfish (Clarias sp.) Protein Hydrolysates as Candidates Matrices for Microbiology Reference Material Kurniawati, Eti; Ibrahim, Bustami; Desniar, Desniar
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 14, No 3 (2019): December 2019
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Fish protein hydrolysate (FPH) is a derivative product of fish proteins containing smaller peptides and amino acids. FPH products have high water solubility, good emulsion capacity, and large expanding ability. With its functional properties, it allows FPH to be used as a raw material in the manufacturing of secondary microbiological reference materials. This study was intended to characterize catfish (Clarias sp.) FPH as a candidate for the matrix of microbial secondary reference. The FPH was prepared through enzymatic hydrolysis, freeze-drying and milling. The hydrolysis processes were carried out using 5% (w/w) papain, 55 Â°C for 5 hours, then the papain activity was stopped by increasing the temperature to 80 Â°C for 20 minutes.The FPH was combined with gelatine, sodium glutamate, glucose solution, and was spiked with Salmonella enteritica sv Enteritidis and freeze-dried. Results showed that catfish FPH was yellowish-white powder with a FPH yield of 11.05%. The proximate analysis of FPH revealed the moisture content of 3.77 Â± 0.12%, ash content of 7.26 Â± 0.03%, protein content of 86.09 Â± 0.17%, and fat content of 1.38 Â± 0.07%. The protein content of the FPH was greater than skim milk (33.42%). Carbohydrate levels of catfish FPH and skim milk were 1.56% and 57.46%, respectively. The best concentration of catfish FPH to perform as a microbiological reference material was 14%, obtained from highest viability of Salmonella bacteria and homogeny. The candidate for reference material were stable at storage temperatures of -20 oC.

Effect of Hydrolyzed Fucoidan from The Brown Seaweed Sargassum Binderi Sonder Towards Human Breast Cancer T47d Cell Lines Sinurat, Ellya; Saepudin, Endang; Qosthalani, Fildzah Alfita
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 12, No 2 (2017): August 2017
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Fucoidan, a sulfated heteropolysaccharide, consists of L-fucose and sulfate ester groups as the main component. Over the past three decades, fucoidan structures and bioactivities have been widely studied. The chemical components (fucose, galactose, small monosaccharides and also the sulfate) and the molecular weights of fucoidans from different brown seaweed species produce different characteristics and structures of fucoidan. The activity of fucoidan against cancer cells has been reported to be affected strongly by their sulfate content and molecular weight. Low-molecular-weight fucoidans tend to have higher solubility and easily penetrate into cancer cells. The objective of this study was to investigate the effect of hydrolyzed of fucoidan on its anti cancer activity againts the breast cancer T47D cells. In this study, the fucoidan from the brown seaweed Sargassum binderi Sonder was extracted using 0.1 N HCl and was depolymerized by acid hydrolysis at various times and concentrations. Result showed that fucoidan hydrolyzed with 1 M trifluoroacetic acid (TFA) for 1.5 hours reached the maximum depolymerization process and resulted in the decrement of molecular weight from 785.12 kDa to 5.79 kDa as well as sulfate content from 18.63% to 8.69%. The IC50 values ofÂ fucoidan and low molecular weight fucoidan against the breast cancer T47D cells were 60.03 mg/mL and 182.34 mg/ respectively. This result indicated that the sulfate content of fucoidan probably affected its anticancer bioactivities.Â

Preface Squalen Bulletin Vol. 14 No. 2 Tahun 2019 Bulletin, Squalen
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 14, No 2 (2019): August 2019
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Preface Squalen Bulletin Vol. 13 No. 1 Tahun 2018 Bulletin, Squalen
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 13, No 1 (2018): May 2018
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Human Health Risk Assessment of Heavy Metals Bioaccumulation In Fish and Mussels from Jakarta Bay Ajeng Kurniasari Putri; Giri Rohmad Barokah; Nuri Andarwulan
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 12, No 2 (2017): August 2017
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Aquatic environment of Jakarta Bay have already been highlighted due to its heavy metals contamination which may lead into food safety concern of fisheries products derived from this region. This research aimed to investigate bioaccumulation level of heavy metals (Pb, Cd, and As) in fish and mussels from Jakarta Bay, as a human risk assessment of seafood consumption. Samples of mussels and fish were taken from Penjaringan, Tarumanegara, Cilincing, and Tanjung Pasir district. Bioaccumulation of heavy metals was analyzed by Inductively Coupled Plasma -Mass Spectrometry technique. Results of the study revealed that metals bioaccumulation in fisheries products were varied, with As (Arsenic) as the highest metal contaminant (0.68 mg/kg, dry weight). Furthermore, estimated daily intake (EDI), target hazard quotient (THQ), total target hazard quotient (TTHQ), and safety limits prescribed by various agencies, showed that consumption of fisheries products from Jakarta Bay considered as relatively safe for human health in total of food ingestion rate of fish 57.34 g/day. These results are expected to serve as a baseline to construct preventive and palliative policies for food safety of fisheries products from Jakarta Bay.

Antibacterial Activity of Sea Cucumbers Harvested from Karimunjawa Delianis Pringgenies
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 8, No 2 (2013): August 2013
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

This research was conducted to discover bioactive compounds in sea cucumbers collected from Karimunjawa as anti-microbial agents against several pathogenic bacteria. Five sea cucumbers species used in this study were Teripang Gamat (Stichopus variegatus), Teripang Nanas (Stichopus chloronotus), Teripang Getah (Bohadschia mamorata), Teripang Emas (Stichopus herrmanni) and Teripang Babi (Bohadschia argus). Sea cucumbers extract were subjected to a series of anti-microbial tests using Staphylococcus aureus, Escherichia coli, Vibrio anguila, Vibrio voinivica, Bacillus subtilis, and Pseudomonas sp. Results showed that in the preliminary study, tissue extract of Bohadschia mamorata and Bohadschia argus were able to suppress the growth of all tested bacteria. Further study used these two sea cucumber extracts, and the results show that Bohadschia mamorata extract at 10 mg/ml showed the largest antibacterial (2.18 mm) towards the pathogenic bacterium Pseudomonas sp., while the smallest antibacterial zone (0.63 mm) was against the growth of Bacillus subtilis. At the concentration level of 20 mg/ml Bohadschia argus extract showed the highest antibacterial zone of 3.68 mm against Staphylococcus aureus and the lowest inhibition zone (1.75 mm) against Pseudomonas sp.

Cytotoxic Activity and Secondary Metabolite Characteristics of Sea Cucumber Actinopyga sp. Methanolic Extract Muhammad Nursid; Afrilia Putri Maharani; Riyanti Riyanti; Endar Marraskuranto
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 11, No 1 (2016): May 2016
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

The objectives of this research were to assess cytotoxic activity and examine characteristic of sea cucumber Actinopyga sp. methanolic extract. Cytotoxicity assay was conducted using MTT method against WiDr (colon cancer) and T47D (breast cancer) cell lines. Actinopyga sp. methanolic extract was characterized by using phytochemical screening, Transform-Infrared (FT-IR) spectroscopy, dan Liquid Chromatography Ion Trap Time of Flight Mass Spectrophotometer (LC-IT-ToF-MS). The result showed that Actinopyga sp. methanolic extract inhibited Widr and T47D cell lines viability with the IC50 value of 55.93 and 87.55 µg/mL, respectively. Functional groups analysis showed the presence of hydroxyl, amine, carboxylic acid, nitrate, amide, sulphur, ester, and ether. LC-IT-ToF-MS analysis of crude extract showed that it containing many steroids compound.

Screening of Antibacterial and Antioxidant Activity from the Soft Corals Sinularia sp. and Sarcophyton sp. Origin Palu Bay, Central Sulawesi, Indonesia Tanod, Wendy Alexander; Dewanto, Didit Kustantio; Ndobe, Samliok; Riyadi, Putut Har; Putra, Masteria Yunovilsa
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 14, No 2 (2019): August 2019
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

This study aimed to evaluate the potential antibacterial and antioxidant activities of Sinulariasp. and Sarcophyton sp. from the Palu Bay, Central Sulawesi, Indonesia. Soft corals were identified as Sinulariasp. (SC1), Sinularia sp. (SC2), andSarcophytonsp. (SC3). Antibacterial activity was examined using agar diffusion well method. Antioxidant activity was measured by the DPPH radical scavenging method. The samples were macerated in MeOH: DCM. The crude extracts were partitioned with DCM, EtOAc, and BuOH. The crude extract of Sinulariasp. (SC2) showed a very strong antibacterial activity as it was able to inhibit the growth of Staphylococcus aureusand Escherichia coliup to 10 mg/mL. Sinularia sp. (SC1) crude extract showed strong activity againstS. aureus, whereas it showed moderate against E. coli.Sarcophyton sp. (SC3) crude extract showed moderate activity against S. aureus, whereas it showed weak against E. coli. The partition fractions of the three soft coral extracts had the potential to be a potent antioxidant agent.

Growth Hormone, Nitrogen and Potassium Content in the Formulated Solid Waste from Agar Processing for Fertilizer Application Jamal Basmal; Muhamad Luthfian Henrida; Rinta Kusumawati; Nurhayati Nurhayati
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 14, No 3 (2019): December 2019
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

The solid waste from agar processing industries in Indonesia is enormous. The waste contains numbers of macro and micro nutrient including growth hormone that can be a good source for plant fertilizer. Utilization of solid waste obtained from agar processing into fertilizer has been carried out by mixing with Sargassum powder, fish silage and Eucheuma cottonii pasta. Various concentrations of the solid waste (13, 19, 22 and 25%) and the Sargassum powder (0, 3, 6, 9, and 12%) were used in this study to obtain optimum nutrients in the formulated fertilizers i.e. growth hormone, total nitrogen and potassium. Growth hormone were analyzed using high-performance liquid chromatography (HPLC), while N content by Kjeldahl methods and K content analyzed according AOAC.The results showed that the optimum formulation was obtained from the combination of 25% solid waste and 0% Sargassum powder, which contained the highest growth hormones i.e. auxins (IAA) 46.75 ppm, gibberellin (GA3) 22.25 ppm, cytokinin-zeatin 18.50 ppm, and cytokinin-kinetin 61.00 ppm. While the potassium content was 0.43±0.001 ppm and the total nitrogen was 0.53± 0.06%. This result meets the requirements of the Minister of Agriculture Regulation No. 261/KPTS/SR.310/M/4/2019 regarding the requirements for total N in organic fertilizer. However, the total K+ does not meet the requirement. Therefore, enrichment with other organic raw material is still needed.

Page 21 of 37 | Total Record : 363

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