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All Journal Jurnal Ilmu dan Teknologi Kelautan Tropis ILMU KELAUTAN: Indonesian Journal of Marine Sciences Journal of Marine Research Prosiding Indonesian Journal of Biotechnology Aceh Journal of Animal Science Jurnal Akuatika Indonesia Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Jurnal Biologi Tropis Molekul: Jurnal Ilmiah Kimia Journal of Fisheries & Marine

Riyanti Riyanti

Universitas Jenderal Soedirman

Author-ID : 6725911

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Energy Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Library & Information Science Materials Science & Nanotechnology

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DETEKSI GEN NRPS AKTINOMISETES SIMBION RUMPUT LAUT DAN KARANG LUNAK , Riyanti; Aziz, Saefuddin; Sabdono, Agus; Radjasa, Ocky Karna
Prosiding Vol 3, No 1 (2012)
Publisher : Prosiding

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Aktinomicetes merupakan bakteri Gram positif dengan kandungan GC yang tinggi dan dikenal sebagai penghasil senyawa metabolit sekunder. Namun potensi aktinomicetes laut terutama dari lingkungan laut masih sangat terbatas dikembangkan. Penelitian yang telah dilaksanakan menyatakan bahwa telah diketahui adanya asosiasi antara bakteri Gram positif yang diduga kuat adalah aktinomisetes dengan rumput laut dan softcoral. Sehingga dari hasil penelitian ini dapat dilanjutkan untuk deteksi dini gen penyandi penghasil senyawa metabolit sekunder kelas PKSs dan NRPSs dan uji aktivitasnya sebagai antioksidan. Deteksi gen PKS I dan NRPS ini dilakukan untuk mencari isolat aktinomisetes yang mempunyai potensi sistem biosintetik senyawa poliketida dan non ribosomal peptida. Sampel untuk penelitian ini diambil dari perairan Pangandaran, Jawa Barat. Sampel yang diperoleh adalah rumput laut, lamun. Metode yang digunakan dalam penelitian ini mulai dari analisa mikrobiologi (isolasi aktinomicetes, uji Gram) , analisa molekuler (rep-PCR , PCR gen NRPS). Berdasarkan penelitian ini diperoleh total aktinomicetes sebanyak 90, dengan keragaman genetik bervariasi koefisien similaritas hingga 1. Koefisien similaritas 1 menunjukkan kekerabatan genetik yang dekat dan koefisien similaritas kurang dari 1 menunjukkan kekerabatan genetik yang jauh. Hasil PCR gen NRPS diperoleh 6 isolat terdeteksi pembawa gen NRPS.

SINTESIS DAN KARAKTERISASI KARBOKSIMETIL KITOSAN Kurniasih, Mardiyah; Kartika, Dwi; , Riyanti
Prosiding Vol 3, No 1 (2012)
Publisher : Prosiding

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Karboksimetil kitosan merupakan derivate dari kitosan yang diperoleh melalui proses eterifikasi alkalis kitosan dengan asam monokloroasetat. Kitosan diperoleh dari deasetilasi kitin dalam kulit udang putih (Penaeus vannamei). Keberadaan kitin dalam kulit udang tidak dalam keadaan bebas, akan tetapi berikatan dengan protein, mineral, dan berbagai macam pigmen (zat warna). Tujuan penelitian ini adalah untuk mensintesis dan mengkarakterisasi karboksimetil kitosan. Berdasarkan hasil penelitian diperoleh kitosan sebanyak 17,33% (per berat serbuk kulit udang) dan karboksimetil kitosan sebanyak 42% (per berat kitosan). Spektum IR karboksimetil kitosan menunjukan melebarnya serapan dari vibrasi ulur gugus -OH yang tumpang tindih dengan serapan vibrasi ulur âNH serta semakin tajamnya intensitas serapan pita ulur C=O dan vibrasi ulur C-O. Kadar air dari kitosan hasil sintesis sebesar 2,13% sedangkan karboksimetil kitosan sebesar 11,86%. Kadar abu kitosan hasil sintesis sebesar 0,73%, sedangkan kadar abu karboksimetil kitosan sebesar 0,93%. Swelling untuk kitosan hasil sintesis sebesar 163,13% sedangkan untuk karboksimetil kitosan sebesar 182,98 %.

Isolation and Screening of Antimicrobial Producing-Actinomycetes Symbionts in Nudibranch ., Riyanti; Widada, Jaka; Rajasa, Ocky Karna
Indonesian Journal of Biotechnology Vol 14, No 1 (2009)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (120.89 KB)

         The aims of  this study were to isolate and to screen actinomycetes associated with sea slug which have the ability to produce antimicrobial compound, especially against MDR strains. Actinomycetes were isolated from nudibranchs collected from Bandengan coastal waters and the Panjang island, Jepara, Central Java. Actinomycete isolates were assayed for their antimicrobial activity against MDR strains (MDR 6 E. coli, MDR 7 Enterobacter sp., MDR 13 Proteus sp., MDR 14 Staphylococcus sp.). The genetic diversity of the active isolates was analyzed by using repetitive DNA fingerprinting.  Antimicrobial activity was also performed on the  ethyl acetate bacterial extract.  The amplification of Polyketide Synthase-I (PKS-I) and Non-Ribosomal Peptide Synthetase (NRPS) genes was carried out to estimate the genetic potency of actinomycetes. The most active actinomycete isolate was sequenced based on 16S rDNA approach. General profile of antimicrobial substances was analyzed by using Thin Layer Chromatography (TLC). A total 27 isolates were obtained from nudibranchs Jorunna sp. and 12 isolates from Chromodoris sp.  Ten isolates exhibited antimicrobial activity. Five representative isolates were selected based on rep-PCR analysis.  Three ethyl acetate extracts exhibited antimicrobial activity against MDR 7, MDR 13, and MDR 14, except MDR 6. NPC 8 isolates significantly inhibited the growth of the tested strain   and amplified NRPS gene fragment. Molecular identification revealed that isolate NPC 8 closely related to Streptomyces sp with a high homology of 96%.

Analysis of Carotenoids and the Identification of Mangrove Sediment Bacteria of Segara Anakan, Cilacap Riyanti Riyanti; Meris Rahmawati; Nuning Vita Hidayati; Agung Dhamar Syakti; Ocky Karna Radjasa
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 23, No 4 (2018): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

The excessive consumption of artificial dyes can lead to negative effects on human body. Thus, the invention of natural dyes, such as carotenoids, is needed in order to reduce the negative impacts. Carotenoids are yellow, orange, and reddish yellow pigments produced by plants, animals, algae, and microorganisms. This study was aimed to analyze the carotenoid pigments of mangrove sediment bacteria in Segara Anakan, Cilacap, and to identify species of bacteria that can produce carotenoids. Carotenoids were analysed by using Thin Layer Chromatography (TLC) and UV-Vis spectrophotometer. Meanwhile molecular identification of bacteria was carried by 16S rDNA PCR and DNA sequence was analysed through a program called Basic Local Alignment Search Tool (BLAST). The kinship of bacteria was shown in Phylogenetic tree by using Bioedit and MEGA 5 software. Qualitative analysis by using TLC produced several pigments like: β-carotene, β-isorenieraten, lycopene, flavonoids, chlorophyll a, chlorophyll b and a feofitin with Rf value of 0.36-0.95. Quantitative analysis showed that bacteria KH (greenish yellow), KT (dark yellow) and KM (light yellow) produced carotenoids at 95.30 mg.g-1, 110.34 mg.g-1 and 25.349 mg.g-1. KH, KT and KM were suspected to be bacteria known as Streptomyces chartreusis, Bacillus megaterium and Streptomyces chromofuscus with similarity of 99%. Mangrove sediment bacteria had the potential of producing carotenoids as an alternative of eco-friendly natural dyes.

Antibacterial activity of Actinomycetes symbiont with seaweeds: a prosperous agent of animal antibacterial Bertoka Fajar Surya Perwira Negara; Riyanti Riyanti; Bintang Marheani; Aradea B. Kusuma
Aceh Journal of Animal Science Vol 1, No 2: December 2016
Publisher : Syiah Kuala University

Circulation of synthetic antibacterial chemicals which is used continuously can cause resistance to bacteria. Alternative synthetic antibiotics research then comes up as an urgent need. Recently, the discovery of new antibacterial agents is looking for Actinomycetes. Actinomycetes are one of the active marine bacteria which produce secondary metabolites that could be used as antibacterial. The objective of this study was to evaluate the antibacterial activities of Actinomycetes symbiont with Halimeda macroloba, Gelidiella acerosa, and Turbinaria ornata in Pangandaran beach, Indonesia. A total of 41 Actinomycetes were isolated from 3 species of seaweed. Rep PCR amplification method was used to characterize and identify the relationship between samples. Primer BOX A1R (5’- CTACGGCAAGGCGACGCTGACGCTGACG-3’), 27F (5'-AGTTTGATCMTGGCTCAG-3') and 1492R (5'- TACGGYTACCTTGTTACGACTT-3') was used in amplification 16s DNA. Six isolates from the representative cluster dendogram were selected for secondary metabolites extraction using ethyl acetate solvent, meanwhile agar diffusion method was used as Antibacterial activity assay using E. coli, Proteus sp., and Enterobacter sp. as the bacterial test. The results showed that the activity of secondary metabolite extracts of Actinomycetes symbiont with Halimeda macroloba presented the highest inhibition zone on 3 bacterial tests. In the future, it could be a potential antibacterial agent against animal pathogenic bacteria.

Optimizing Conditions to Cholesterol Adsorbed with Carboxymethyl Chitosan Mardiyah Kurniasih; Dwi Kartika; Riyanti Riyanti
Molekul Vol 11, No 1 (2016)
Publisher : Universitas Jenderal Soedirman

A research on optimizing conditions to cholesterol adsorbed have been performed. Optimization was performed by varying: contact time, adsorbent weight and temperature of the system's. A full factorial experimental design was used in this study. Characterization performed on the synthesized chitosan and carboxymethyl chitosan including FTIR, water content, ash content, solubility, porosity, and swelling effect. The results showed that carboxymethyl chitosan able to adsorb cholesterol under conditions optimal adsorbent with cholesterol ratio (1:200) with a contact time of 90 minutes at temperature of 40 °C. Meanwhile, at a temperature of 55 °C carboxymethyl chitosan capable of adsorb cholesterol under conditions optimal adsorbent with cholesterol ratio (1:300) with a contact time of 30 minutes. Chitosan and carboxymethyl chitosan synthesized has a water content of 7.4 and 10.2%, ash content of 0.14 and 2.29%, solubility in distilled water at 1.10-5and 1.98.10-3%, solubility in acetic acid 0.02 and 0.04%, porosity at 88.3% and 88.8%, and swelling at 163.13 and 182.98%.

Cytotoxic Activity and Secondary Metabolite Characteristics of Sea Cucumber Actinopyga sp. Methanolic Extract Muhammad Nursid; Afrilia Putri Maharani; Riyanti Riyanti; Endar Marraskuranto
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 11, No 1 (2016): May 2016
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

The objectives of this research were to assess cytotoxic activity and examine characteristic of sea cucumber Actinopyga sp. methanolic extract. Cytotoxicity assay was conducted using MTT method against WiDr (colon cancer) and T47D (breast cancer) cell lines. Actinopyga sp. methanolic extract was characterized by using phytochemical screening, Transform-Infrared (FT-IR) spectroscopy, dan Liquid Chromatography Ion Trap Time of Flight Mass Spectrophotometer (LC-IT-ToF-MS). The result showed that Actinopyga sp. methanolic extract inhibited Widr and T47D cell lines viability with the IC50 value of 55.93 and 87.55 µg/mL, respectively. Functional groups analysis showed the presence of hydroxyl, amine, carboxylic acid, nitrate, amide, sulphur, ester, and ether. LC-IT-ToF-MS analysis of crude extract showed that it containing many steroids compound.

Investigation on Antioxidant Compounds from Marine Algae Extracts Collected from Binuangeun Coast, Banten, Indonesia Muhammad Nursid; Endar Marasskuranto; Karren Budi Atmojo; Mulyo Pandu Hartono; Maria Diyah Nur Meinita; Riyanti R
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 11, No 2 (2016): August 2016
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v11i2.243

Marine algae contains bioactive secondary metabolites that have potential to be developed as antioxidant. The aims of this research were to investigate antioxidant activity and total phenolic compound of marine algae collected from Binuangeun Beach, Banten and to characterize antioxidant compounds from selected algae species. Antioxidant activity was determined by 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) assay and Ferric Ion Reducing Antioxidant Power (FRAP) Assay, while total phenolic content was estimated by Folin-Ciocalteau method. Isolation of bioactive antioxidant compound was performed using SiO2 column chromatography and preparative high performance liquid chromatography (HPLC). The antioxidan activity evaluation was conducted to methanol extracts from 20 species (7 Chlorophyta, 9 Phaeophyta and 4 Rhodophyta). Results showed that Padina australis extract was found to be the most active. The major bioactive antioxidant compound was identified as fucoxanthin and a polar compound that was suspected as phenolic compound. The extracts of P. australis presented the highest phenolic content (58.59 mg GAE/g). A significant correlation between antioxidant capacity and total phenolic content was found, indicating that phenolic compounds are the major contributors to the antioxidant properties of P. australis.

The Growth Rate of Cyphastrea Coral Fragment Under Controlled Condition Reynaldi Surya Putra; Adrian Rahman Septiandi; Riyanti Riyanti
Jurnal Biologi Tropis Vol. 23 No. 2 (2023): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i2.5897

The role of corals was very important in coastal and marine ecosystems, whether in terms of education, ecology, and socio-economy. Coral growth can be used as an indicators of marine ecosystem health. This study was aimed to observed survival rate and the growth rate of Cyphastrea coral fragments. Cyphastrea coral fragments growth rate was observed in ex-situ sites and was conducted over a period of 3 months. The micro-fragmentation method was applied in this study. Several factors such as water temperature, light levels, and nutrient concentrations were measured and controlled throughout the study. The growth rate of Cyphastrea coral fragments was measured using ImageJ software. Result of this study exhibited that survival rate of Cyphastrea was 100% and the growth rate of Cyphastrea was 0.012 cm2/month. This study provides important information on the factors that affect the growth rate of Cyphastrea coral fragments in ex-situ sites. The result of this study can be used to develop more effective strategies for coral reef restoration.

Laju Pertumbuhan Dan Survival Rate Fragmen Karang Hias Echinopora lamellosa Secara In Situ Di Perairan Penginuman, Gilimanuk Kurniawan, Hertanto Dwi; Septiandi, Adrian Rahman; Ardiansyah, Anwan Rahmat; Damanik, Gabriel Dala Bona Putra; ., Riyanti
Journal of Marine Research Vol 13, No 2 (2024): Journal of Marine Research
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jmr.v13i2.40390

Ekosistem terumbu karang memiliki nilai penting dalam kehidupan, baik dari segi ekologi maupun nilai ekonomi. Perairan Penginuman, Gilimanuk merupakan salah satu lokasi dengan kondisi terumbu karang yang buruk. Echinopora lamellosa merupakan spesies karang hias yang memiliki pertumbuhan cepat sehingga dapat digunakan dalam upaya pemulihan karang. Laju pertumbuhan karang dapat diukur dengan menghitung penambahan tinggi maupun luas areanya. Tujuan penelitian ini adalah mengetahui laju pertumbuhan dan survival rate karang E. lamellosa. Metode penelitian ini menggunakan analisis kuantitatif dengan pengambilan data secara langsung selama 4 bulan dengan interval waktu setiap 1 bulan sekali. Foto karang sampel didigitasi menggunakan software ImageJ untuk mengetahui luas area pertumbuhannya. Hasil yang diperoleh yaitu kenaikan rata-rata area pertumbuhan per bulan yaitu berkisar antara 1.04 – 2.79 cm2. Hasil perhitungan tingkat kelangsungan hidup E. lamellosa bernilai 66.67%. Budidaya karang secara in situ yang dilakukan di perairan Penginuman, Gilimanuk termasuk dalam kategori berhasil karena karang tumbuh lebih cepat dan memiliki kelangsungaan hidup yang cukup tinggi. Coral reef ecosystems have important role in life, both from an ecological and economic values. Water of Penginuman, in Gilimanuk is one of the locations with poor conditions of its coral reef. Echinopora lamellosa is one of ornamental coral species that has fast growth so they can be used in coral recovery system. Coral growth rate can be known by measuring calculating the growth of their height and wide area of surface. The purpose of this research was to find out the growth rate and survival rate of E. lamellosa corals. Quantitative analysis with direct data collection was used in this research method for 4 months with intervals once per month. Coral photos sample were digitized using ImageJ software to determine the wide area of their growth. The data show an increase in average monthly growth, which varies from 1.04 to 2.79 cm2. The calculated E. lamellosa survival rate was 66.67%. The in situ method of coral culture in Penginuman Waters, Gilimanuk, may be considered effective because the corals grow more quickly and have a high enough survival rate.

Co-Authors Abrar , Muhammad Adrian Rahman Septiandi Afrilia Putri Maharani Agung Dhamar Syakti Agus Sabdono Anggiaputra, Nathanael Steffano Budi Aradea B. Kusuma Arbanto, Bonifasius Ardiansyah, Anwan Rahmat Arifin , Reyhan Ariqa Iriani, Fringgar Athar, Muhammad Raihan Aulia, Indira Hauriina Bertoka Fajar Surya Perwira Negara Bintang Marheani Damanik, Gabriel Dala Bona Putra Dewi Ruswanti, Corina Dwi Kartika Dwi Kartika Endar Marasskuranto Endar Marraskuranto Fadlan, Azhardhya Norr Muhammad Fitriani, Nabila Ayu Giyanto Hanafi Hanafi Hindami, Rio Irreniza, Kayla Yasmin Izuan, Muhammad JAKA WIDADA Jeriansyah, Jejen Jerry Winata, Marcellinus Karren Budi Atmojo Khusna, Faridatul Kurniawan, Hertanto Dwi Lathifah, Nur Laila Linuz Mambrasar, Ronald Mardiyah Kurniasih Mardiyah Kurniasih Maria Diyah Nur Meinita Meris Rahmawati Muhammad Nursid Mulyo Pandu Hartono Nuning Vita Hidayati Ocky Karna Radjasa Orgenese Permata Putri , Novita Putra, Mochamad Iqbal Herawata Rachmawati, Siti Fitria Rahman Ritonga, Abdul Reynaldi Surya Putra Rifat Muharam , Muhammad Rikoh Manogar Siringoringo Riviani , Riviani Saefuddin Aziz Sari, Ni Wayan Purnama Septiandi, Adrian Rahman Syahrodji, Putri Saqina Emilia Wijaya, Abhimantara Agung Pradnya

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