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INDONESIA
ANNALES BOGORIENSES
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : 05178452     EISSN : 24077518     DOI : -
Core Subject : Health, Science, Agriculture, Engineering,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Medicine & Pharmacology
The Annales Bogorienses (ISSN: 0517-8452, E-ISSN: 2407-7518) is a peer-reviewed Journal that is published biannually. First published in 1955, it is now one of the oldest scientific journal in the nation. The Annales Bogorienses publishes original articles in basic and applied research as well as critical reviews and short communication in the fields of life sciences with the emphasis in biotechnology, molecular biology, and biochemistry.
Arjuna Subject : -
Articles 540 Documents
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Editorial Boards AB Vol 21 No 1 (2017) Desriani, Desriani
ANNALES BOGORIENSES Vol 21, No 1 (2017): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (210.296 KB) | DOI: 10.14203/ab.v21i1.304

Abstract

Phenotypic Screening of Ds transposon and Activation-tag Insertional Mutant Rice Population for Drought and Salinity Tolerant Related Traits Nugroho, Satya; Zannati, Anky; Situmorang, Apriadi; Windiastri, Vincentia Esti; Widyajayantie, Dwi; Pantouw, Carla F.; Astuti, Dwi; Indrayani, Sri; Rahmawati, Syamsidah; Mulyaningsih, Enung Sri; Santoso, Tri Joko; Trijatmiko, Kurniawan Rudi
ANNALES BOGORIENSES Vol 15, No 1 (2011): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1234/56

Abstract

The studies of rice genes through functional genomics are greatly facilitated by the availability of the complete genome sequences, including the complete physical map of the japonica rice cv. nipponbare. Using the Ac and Ds transposon, that are capable of transposition in various heterologous plants including the monocotyledon rice,  combined with the enhancer element for generating activation taged lines, it is possible to discover and isolate functional genes involved in various important agronomical traits; such as those involved in abiotic stress tolerance (drought, high salt) and biotic stresses diseases and pests. We have developed 1,785 first (T0) generation of mutant nipponbare rice by transposons Ac/Ds insertions containing activation-tag, generated by transformation using Agrobacterium-mediated method. Currently, we have generated approximately 1,000 stable lines with transposon Ds and activation-tag insertion ready for screening. Efficient screening methods for mutant Nipponbare rice lines have been established for agronomically important mutant traits. Among the new phenotypes related to important agronomical traits observed were drought and salt tolerant or sensitive, stunted and robust growth, variable root penetration and other interesting traits such as reduced tillering, rolled leaf and thin tiller.   Keywords: rice, insertion mutant, gene discovery, drought, salinity
Pcr Amplification of Ornithine Decarboxylase (ODC) Gene Fragment from Tobacco (Nicotiana tabacum L.) cv. Temanggung Djajanegara, Ira; Pambudi, Sabar; Lestari, Retno; Artanti, Nina
ANNALES BOGORIENSES Vol 9, No 2 (2004): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1234/11

Abstract

In order Lo create an antisense construct of the gene encoding Ornithine Decarboxylase (ODC) from tobacco (Nicotiana tabacum L.) cv. Temanggung, the target gene must be isolated. In this paper. we present the PCR amplification of a fragment from putative gene encoding ODC from tobacco cv. Temanggung. Leaf genomic DNA was i olated and used as the template for PCR. PCR optimization was done by adjusting the annealing temperature and the cycle number. Verification of the fragment obtained was also done using the second primer pairs .  
Front Cover AB Vol 19 No 2 (2015) lisdiyanti, puspita
ANNALES BOGORIENSES Vol 19, No 2 (2015): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ab.v19i2.281

Abstract

Response of Increasing NaCl Concentrations on Growth and Proline Content of Tacca leontopetaloides cultured in vitro Martin, Andri Fadillah; Hapsari, Betalini Widhi; Ermayanti, Tri Muji
ANNALES BOGORIENSES Vol 19, No 1 (2015): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ab.v19i1.81

Abstract

Tacca leontopetaloides (L.) Kuntze which belongs to family Taccaceae is one of tuberous plant useful as an alternative food. This plant contains starch (amylose and amylopectin) similar to that of potato. In addition, this plant also contains secondary metabolites potential for diarrhea, dysentery, and anticancer. In Indonesia, this plant grows at coastal areas i.e. in Sukabumi, Yogyakarta, Garut and Karimunjawa, so this plant was suspected to have high tolerance on salinity stress. The purpose of this research was to investigate the effect of increase in NaCl concentrations on growth and its proline content of T. leontopetaloides grown in vitro. In vitro shoot tips were cultured on MS medium (Murashige & Skoog, 1962) supplemented with NaCl at concentrations of 10; 25; 50; 75; 100; 150 and 200 mM, respectively. After six weeks in culture, shoots height, shoots number, leaves number, fresh weight, as well as their proline content were recorded. The results showed that fresh weight of shoots grown on MS medium supplemented with NaCl from 10 to 100 mM was hogher compared to those of the control treatment. Fresh weight decreased when shoots were cultured on MS medium supplemented with NaCl at more than 100 mM. Height of shoots, number of shoots, and number of leaves decreased along with the increase of NaCl concentrations. The best medium for growth was MS medium supplemented with 25 mM NaCl. In this medium proline content was 15.7 umol/g FW. Proline concentrations increased along with the increase of NaCl concentrations. LD50 value was 146 mM NaCl. In conclusion that on MS medium without addition of plant growth regulators, T. leontopetaloides shoot culture was sensitve to the increase of NaCl concentration.
Optimization of Somatic Embryogenesis Induction of Cassava (Manihot esculenta Crantz) Susanti, Idha; Suharsono, Suharsono; Widyastuti, Utut; Siregar, Ulfah Juniarti; Tjahjoleksono, Aris
ANNALES BOGORIENSES Vol 21, No 2 (2017): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ab.v21i2.223

Abstract

The embryogenesis (SE) has important role for genetic engineering of cassava (Manihot esculenta Crantz). However, the success of SE induction depend on plant growth regulator s (PGR)s and treatment enriched in induction media.  This experiment tried to induce callus formation of cassava from several in vitro explants: immature leaf, apical bud, and internode; and to develop somatic embryogenesis of cassava in several media enriched with tyrosine and copper sulphate (CuSO4) added into media enrich with picloram as treatment.  Different response of explants source to callus induction treatment from those three varieties in callus induction as well as friable callus formation were found in this experiment. The best medium to induce varied with variety; MS media supplemented 12 mg/L picloram + 0.5 mg/L CuSO4 was the best for “Adira 4” and  half MS and half GD media supplemented 12 mg/L picloram + 100 mg/L tyrosine for “Malang 6”.  All treatments resulted somatic embryo which developed indirectly and in morphologically normal somatic embryos
Molecular Identification of Microalgae BTM 11 and its Lectin Isolation, Characterization, and Inhibition Activity Mustopa, Apon Zaenal; Isworo, Rhestu; Nurilmala, Mala; Susilaningsih, Dwi
ANNALES BOGORIENSES Vol 20, No 2 (2016): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ab.v20i2.251

Abstract

BTM 11 is unknown species of microalgae, but has active compounds that can inhibit viruses. One of proteins produced by microalgae is a lectin. Lectin is a carbohydrate-binding protein found in various microalgae that show antiviral and antibacterial activity. The purpose of this study was to perform identification of the species of microalgae BTM 11, isolation, characterization, and assay of lectin inhibitory activity. Microalgae BTM 11 was identified to have homology with Cyanobacterium 99% and Geitlerinema sp 98%. Lectin of microalgae BTM 11 was isolated by ammonium sulfate precipitation of 75% with a molecular weight of 17 kDa. Lectin protein activity of microalgae BTM 11 was able to inhibit the enzyme activity of RNA helicase hepatitis C by 57.90% and 27.55%. In addition, the protein was able to suppress the activity of Staphylococcus aureus ATCC 6538, E. coli EPEC K.1.1. and Salmonella typhii ATCC 25241. Activitiy of lectin was stable at 30 °C and was unaffected by the action of the enzyme. These results indicate that lectin of microalgae BTM 11 could be a alternative to antiviral and antibacterial proteins.
Back Cover AB Vol 17 No 1 (2013) Lisdiyanti, Puspita
ANNALES BOGORIENSES Vol 17, No 1 (2013): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (509.744 KB) | DOI: 10.1234/170

Abstract

Segregation Analysis of Transgenic Rice Plants CV Rojolele Harboring Cry1B Gene and Plant Selection for Potential to Yellow Stem Borer Estiati, Amy; Sulistyowati, Yuli; Zahra, Fatimah; Nurhasanah, Ade Nena; Rahmawati, Syamsidah; S-Loedin, Inez H
ANNALES BOGORIENSES Vol 16, No 1 (2012): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (698.51 KB) | DOI: 10.1234/42

Abstract

Transgenic rice plants harboring resistant gene to yellow stem borer, cry1B gene had been obtained. However, the cry1B gene in second generation of transgenic rice lines still segregating following Mendelian ratio 3:1. For further use in the breeding programs, it is important to ensure that the gene is dominant gene and the plants are homozygous for  cry1B gene. Selection  of homozygous transgenic rice lines containing  cry1B  gene at third, fourth and fifth generation had been conducted by PCR.  The presence of  cry1B  gene was determined by showing the PCR product of 1.9 kb. Segregation analysis proved that six transgenic rice  lines i.e. 3R7.8.15.1, 3R7.8.15.9, 3R7.8.15.10, 3R7.8.15.15, 3R7.8.15.21, 3R7.8.15.29 are homozygous lines for  cry1B  gene.   Moreover, bioassay studies at vegetative stage on six homozygous transgenic rice lines showed that  these transgenic rice lines are potential resistant to yellow stem borer comparing with non-transgenic plants, with the score of 0 (indicated no symptom) for six transgenic lines and score of9 (more than 60% damaged tillers ) for non-transgenic plants. However, to confirm the efficacy of  cry 1B  gene to yellow stem borer in naturalcondition, confined field trial in endemic area of yellow stem borer should be conducted. Keywords: bioassay, cry1B gene, transgenic rice, yellow stem borer
Optimization of Substrate and Starter Cell Concentrations for Dibenzothiopene Biodegradation by Indigeneous Marine Bacteria Mauricauda olearia LBF-1-0009, Alcanivorax xenomutants LBF-1-0018, and Stakelama pacifica LBF-1-0031 Yetti, Elvi; Thontowi, Ahmad; Yopi, Yopi
ANNALES BOGORIENSES Vol 21, No 2 (2017): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (646.12 KB) | DOI: 10.14203/ab.v21i2.300

Abstract

Dibenzothiophene (DBT) and its derivatives have been widely used as model organic sulfur compounds in petroleum, included their biodegradation process. The abilities of microorganisms to degrade pollutants are significantly influenced by various factors such as microbial species, nutrients and environmental parameters. In this research, we carried out further study to determine optimal condition for DBT biodegradation regarding with substrate and strains cell concentration by several indigenous marine bacteria from Indonesia. These three isolates were belong to Mauricauda olearia, Alcanivorax xenomutants, and Stakelama pacifica, with homology result 99% each. Optimal dibenzothiophene as substrate reached by all isolates is 100 ppm, while cell concentration or microbial numbers that gave highest growth for all isolates is 20 based on conversion of OD600 nm measurement.  

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