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Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal
Published by Universitas Jenderal Soedirman
ISSN : 08531629     EISSN : 08531629     DOI : -
Core Subject : Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science
Majalah Ilmiah Biologi Biosfera : A Scientific Journal merupakan peer reviewed jurnal yang diterbitkan oleh Fakultas Biologi Universitas Jenderal Soedirman. Media ini mewadahi hasil-hasil penelitian di bidang biologi tropika yang terbit tiga kali setahun (Januari, Mei, September).
Arjuna Subject : -
Articles 342 Documents
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Studi Awal Komunitas Ordo Anura di Kawasan Ekowisata Sawangan, Magelang, Jawa Tengah Tony Febri Qurniawan; Hestin Ambar Asti; Rury Eprilurahman
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 27, No 3 (2010)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2010.27.3.201

Abstract

Frogs and toads (Amphibia: Anura) were very sensitive to environmental changes. Due to their sensitivity, usually they can be used as indicator for environmental changes. On the other hands, there has been only limited data on the diversity and distribution of frogs and toads in Java. This could lead to the reducing population of frogs and toads in Java. Based on those facts, this research was aimed to collect data on species diversity, species richness, species composition and species distribution of frogs and toad from Sawangan Ecotourism, Magelang, Central Java Province. This study was expected to complete the database of anurans in Central Java. The research was conducted from June to July 2009, mostly at night. The VES (Visual Encounter Survey) method combined with 200 m transects were used for sampling. Seven species of anurans were recorded during the survey. They were Duttaphrynus melanostictus, Limnonectes kuhlii, Limnonectes microdiscus, Microhyla achatina, Odorrana hosii, Hylarana chalconotus and Huia masonii. From those seven species, only three (Huia masonii, Microhyla achatina and Limnonectes kuhlii) were known as endemic of Java. 
Transport Nutrien penyebab Eutrofikasi dari Daerah Tangkapan Air Waduk Mrica Banjarnegara Agatha Sih Piranti; Soedarmadji Soedarmadji; Gentur Waluyo; Suwardi Suwardi
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 32, No 1 (2015)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2015.32.1.297

Abstract

The transport of nutrien causing eutrophication in water from catchment area especially fosforus (P) through runoff is determined by type of landuses, slop, soils type, and land cover (Jeje,2006) . The research was conducted using a survey method. The sampling technique was done by a purposive sampling on any flooding in the rivers that pass through areas with specific land use (settlement, forest, plantations, agricultural fields and rice field). The main parameters were river discharge and the content of nutrients causing eutrophication that was phosphorus (P) in rivers. The P loadingfrom cacthment area to Mrica reservoir was 11,526,706.14 kg/year and the load allocation (LA) was 3,833,587.70 kg/year. The reservoir operational of power generating dan flushing activity were able to manage the LA up to 1,147,329.09kg/year, so the TMDL of Mrica reservoir become 4,980,916.78kg/year. It was concluded that the highest transport of P was from paddy field and then followed by respectivelly agricultural (potato) field, plantation (perhutani) field, settlement area, and forest area.
Variasi Sekuens Gen Mitokondrial Sitokrom C Oksidase I dari Siput Lola (Trochus niloticus) Agus Nuryanto; Dedy Duryadi Solihin
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 23, No 1 (2006)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2006.23.1.143

Abstract

A study on sequence variation in the mitochondrial DNA of Trochus niloticus has been conducted. Polymerase chain reactions (PCR) technique was applied to amplify cytochrome c oxidase I gene of 533 bp and ABI 310 aotomatic DNA sequencer was used to sequence the PCR product.  This study was aimed to know the genetic variation of T. niloticus and the suitability of partial sequence of cytochrome c oxidase I gene as a molecular marker in the genetic study.  The results showed that from two T. niloticus specimens, 12 polymorphic sites were detected yielding 2 haplotypes. This indicated a highly genetic variation in thecytochrome c oxidase I (COI) gene of T niloticus.  In addition, cytochrome c oxidase I gene was proved to be a suitable marker for the genetics studies of various species of organisms.
Karakterisasi Ganoderma spp. di Kabupaten Banyumas dan Uji Peran Basidiospora dalam Siklus Penyakit Busuk Batang Nuniek Ina Ratnaningtyas; Siti Samiyarsih
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 29, No 1 (2012)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2012.29.1.233

Abstract

Ganoderma sp., one of fungi belonged to the family Ganodermataceae, order of Aphyllophorales, class of Basidiomycetes is very widely spread.  This fungus is a soil-borne, and has parasitic and saprophytic features which are interesting because of their two contradictory roles namely harmful and beneficial effects.  As a plant parasite, Ganoderma is able to cause root and stem rot of tropical perennial crop plantation and forest which cause serious losses.  The fungus is well known as white rot fungus which is able to cause a wood rotten by lignin destruction.  On the contrary, the fungus has some advantages such as medical potentials.  In this study, some collections and characterization of Ganoderma spp. found in Banyumas has been conducted.  The technique used for sampling Ganoderma spp. mushroom is a survey with Purposive Random Sampling method.  Samples were taken from nature in the area of Banyumas Regency and its natural substrate were noted. Characterization based on the macromorphology and micromorphology was done for grouping and identification.  Micromorphology observations were done by slicing and painting samples with plants microtechnique method.  The result showed that there were 43 Ganoderma spp isolates, distributed from low to high lands of 100 - 500 m above sea level.  The most fungi were found on the high lands, in Baturraden sub district, which included 16 isolates.  The interesting finding is the discovery of Ganoderma spp. which attacked flamboyant plant (Delonix regia) in West Purwokerto sub district.  Based on micromorphology observations, these fungi form a basidium and basidiospore inside and below as well as the surface of the fruit body.  Some species were also found having such phenomenon so it needs further research on the role of basidiospora in the cycle of stem rot disease, especially basidiospora role in the initiation of diseases infection.  As the soil infecting mushroom, study of the disease spreading is focused more on the role of the mycelium found in the remaining plants as infecting the material.  Basidiopore role in the spread of the disease is rarely studied, but actually basidiospore is a potential source of genetic variation.
Deteksi Resistensi Amoxicillin Helicobacter pylori pada Pasien Dispepsia Indah Sulistiyawati; I Gede Arinton; Hendro Pramono
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 33, No 3 (2016)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2016.33.3.308

Abstract

Amoxicillin is one of the antibiotics that commonly used on treatment of H. pylori infection. pbp1A gene mutation in H. pylori is a dominant cause of amoxicillin resistance. This study was aimed to evaluate the presence of H. pylori in patients with dyspepsia by using non-invasive method i.e. H. pylori stool antigen (HPSA) and invasive method i.e. pbp1A gene amplification, and also evaluate the amoxicillin resistance of H. pylori by assessing the pbp1A gene mutations. The samples were  26 faeces and 26 gastric biopsies of patients with dyspepsia from the Internal Disease of Prof. Dr. Margono Soekardjo Hospital in Purwokerto. DNA amplification performed by using polymerase chain reaction (PCR) to detect the presence of amoxicillin resistance encoding gene i.e. penicillin binding protein (pbp1A). Sequencing of the DNA sample was performed at the First Base Malaysian Company, to analyze the existence of a point mutation. DNA sequencing analysis of 12 samples showed the presence of a mutations in pbp1A gene from 2 samples, in the third motive of pbp i.e. amino acid changes, Alanine 599 substituted to Threonin and Threonin 592 to Alanine. Those mutations become a dominant risk factor for resistance of H. pylori, toward the bacterial peptidoglycan synthesis. In this research, it was known that the detection of H. pylori infection by using PCR remains more accurate and specific method. The presence of H. pylori mutant strains in this study may becomes the risk factors of resistance to amoxicillin treatment.     
Penggunaan Botes AHW sebagai Alat Siram Tetes dalam Pembudidayaan Cendana di Kabupaten Belu, NTT Albert Husein Wawo; Rochadi Abdulhadi
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 22, No 2 (2005)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2005.22.2.94

Abstract

One of factors  that  causes the failure of the sandalwood cultivation in East Nusa Tenggara Province is drought of seedlings after planting. This research  has been conducted to study the use of Botes-AHW as an equipment of drip watering on sandalwood cultivation and to evaluate the water requirements in seedling growth after planting. The results  showed that   the use of water was more conserved by employing the Botes-AHW in sandalwood cultivation  than the traditional watering system.  The average of water requirements  in seedling growth (several months after planting)  is 10 liters  per seedling per month.
Purification of Biotransformation Products of Cis-Isoflavan-4-Ol by Biphenyl Dioxygenase of Pseudomonas pseudoalcaligenes Kf707 Strain Expressed in Escherichia coli Tri Ratna Sulistiyani; Agus Budiyanto
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 27, No 2 (2010)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2010.27.2.192

Abstract

Isoflavone has multiple beneficial effects on human health, especially as antioxidant and anticancer. Biotransformation of two enantiomers (CE1 and CE2) of cis-isoflavan-4-ol by E. coli JM109 (pJHF108) carrying a biphenyl dioxygenase gene from P. pseudoalcaligenes KF707 produced two products and they were designated as CM1 and CM2. They had retention time at 11.9 and 14.6 min, respectively, and same absorption peaks at 204, 220 and 275 nm. CM1 and CM2 had [M-H2O+H]+  at m/z 225. Based on the molecular mass, hydrolysis products, and previous report, this study proposed that epoxidation occurred on cis-isoflavan-4-ol. Chloroform extraction was done to improve the stability of CM1 and CM2.
Metode Alternatif Penyimpanan Udang Segar dengan Nisin dari Lactococcus lactis sebagai Pengawet Alami Dyah Fitri Kusharyati; P Maria Hendrati
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 24, No 3 (2007)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2007.24.3.284

Abstract

Nisin is a Lactococus lactis metabolite, one of natural preservatives against microbes. The antimicrobial inhibition is influenced by its concentration and contact time with the targeted microbes. The aim of the study was to know the concentration of L. lactis extract and optimal time of soaking the growing shrimp bacterial in refrigerator temperature for nine days. This study was conducted experimentally applying factorial design. The first factor was soaking duration, i.e. 0 minute (P0), 30 minutes (P1), 60 minutes (P2), and 90 minutes (P3), while the second factor was concentration levels of metabolite of L. lactis extract. The results showed that the metabolite has an inhibitory effect on shrimp bacterioal in dosed dependent way. The best performance of inhibition was detected at 60 minute-soaking duration.
Konservasi Eksitu Jenis Begonia Alam Pegunungan Sumatra di Kebun Raya Cibodas Muhammad Efendi
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 35, No 2 (2018)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (608.885 KB) | DOI: 10.20884/1.mib.2018.35.2.628

Abstract

Kegiatan konservasi secara eksitu Begonia alam dari pegunungan telah dilakukan di Kebun Raya Cibodas sejak tahun 1930. Keanekaragaman jenis dan persebaran Begonia Sumatra didata berdasarkan koleksi Kebun Raya Cibodas, ditambah dengan spesimen herbarium (Universitas Andalas (ANDA) dan Cianjur Hortus Tjibodasensis). Informasi perbanyakan, hama dan penyakit tanaman didata selama kurun waktu dua tahun (2015-2017). Lima belas Begonia alam Sumatra telah dikonservasi di Kebun Raya Cibodas. Secara umum, Begonia dapat diperbanyak secara vegetatif maupun generatif. Perbanyakan secara in vitro dilakukan pada B. leuserensis, B. atricha dan B. scottii, jenis begonia endemik dan langka di Sumatra, telah dilakukan untuk meningkatkan jumlah begonia tersebut dalam botol kultur. Permasalahan konservasi Begonia di Kebun Raya Cibodas didiskusikan secara singkat.
Perilaku Tumbuh Kantong Semar (Nepenthes mirabilis Druce) di Habitat Alaminya, Taman Nasional Kutai, Kalimantan Timur Tri Handayani; Inggit Puji Astuti
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 22, No 3 (2005)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2005.22.3.176

Abstract

Nepenthes mirabilis has been widely known as an insectivorous plant species. A study on the growing behaviour of N. mirabilis in Teluk Kaba, Kutai National Park has been carried out. As many as approximately 840 individuals consisting of 307 rosettes and 533 mature plants were found. Variation in tendril’s position was observed in nine rosettes plants and in 22 mature plants. On the other hand, variations in leaf colour appeared in eight individuals. Variations in colour of pitcher and tendril were found in 17 individuals. The natural habitat of N. mirabilis was very specific, and it was also noted that 13 species were assosiated with this plant species. Further result will be discussed in this paper.

Page 17 of 35 | Total Record : 342

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