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Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal
ISSN : 08531629     EISSN : 08531629     DOI : -
Majalah Ilmiah Biologi Biosfera : A Scientific Journal merupakan peer reviewed jurnal yang diterbitkan oleh Fakultas Biologi Universitas Jenderal Soedirman. Media ini mewadahi hasil-hasil penelitian di bidang biologi tropika yang terbit tiga kali setahun (Januari, Mei, September).
Arjuna Subject : -
Articles 342 Documents
Efek NAA dan BAP terhadap Pembentukan Tunas, Daun, dan Tinggi Tunas Stek Mikro Nepenthes ampullaria Jack. Heti Sartika Sari; Murni Dwiati; Iman Budisantosa
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 32, No 3 (2015)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2015.32.3.343

Abstract

This study aimed to know the interaction between NAA and BAP as well as to obtain the best combination of both treatments in promoting the growth of Nepenthes ampullariamicrocutting.An experiment arranged in a factorial Randomized Complete Block Design (RCBD) wasapplied. Stem segments were used as blocks, where block I was the first segment followed by the next two segments as block II and III respectively. Two factors, i.e. NAA concentrations (0, 5, 10, 15 µM) and BAP concentrations (0, 9, 18, 27 µM) were employed giving rise to 16 combination of treatments. Each treatment combination was replicated threetimes resulting in 48 experimental units. The parametersmeasured were date of shoot initiation, date of root initiation, shoot number, leaf number, root number, length of longest leafand shoot height. The results showed that interactionbetween NAA and BAP in promoting N. ampullariamicrocutting growth was observed.  Combination between NAA of 0 µM and BAP of 18 µMwas found to be the best in promoting N. ampullariamicrocutting growth.Meanwhile, combination between NAA 0 µM and BAP 27 µM was recommended to promote shoot number of N. ampullaria.
Studi Perbandingan Komunitas Ikan dan Udang Daerah Hilir ke Arah Hulu pada Dua Sungai di Kawasan Cagar Alam Leuweung Sancang Garut-Jawa Barat Gema Wahyu Dewantoro; Edi S Zulham; Asep Rudi P
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 22, No 1 (2005)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2005.22.1.89

Abstract

A comparative study on fish and shrimp community from downstream to upper area at two river in natural preserve Leuweung Sancang, Garut, West-Java has been conducted. The aim of the study was to examine how far influence threat damaged fish and shrimp at both river. The result showed 3 species of fish and 3 species of shrimp at Ciporeang river, and 3 species of fish and 1 species of shrimp at Cipangisikan river.
Penentuan Konduktivitas Listrik dan Frekuensi Karakteristik Sel Ragi dengan Memanfaatkan Proses Dielektroforesis Much Azam; Wahyu Setia Budi; Hermin Pancasakti
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 27, No 1 (2010)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2010.27.1.187

Abstract

Dielectrophorestic (DEP), a phenomenon where small particles such as yeast cells are manipulated by non-uniform electric fields. The net dielectrophoretic force is proportional to the polarisability of the cell and cause this cell moving toward higher field. By measuring velocity of the cell on the various frequency of the electric field, it can determine the electrical conductivity and the characteristic frequency of the yeast cells. The results showed that the conductivity of the yeast cell was (10,0 ± 0,4)10-9 Sm-1  and the characteristic frequency was 60 kHz.
Pemanfaatan Ekstrak Gulma Air untuk Meningkatkan Pertumbuhan dan Produksi Mikroalga Spirulina platensis pada Kultur Skala Laboratorium Christiani Christiani; Hexa Apriliana Hidayah
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 28, No 3 (2011)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2011.28.3.279

Abstract

Microalgae Spirulina platensis is good as natural food for fish. It consists of the highest protein, that is 70% of the dry weight.  On the other hand, it can be used as alternative of healthy food and herbal medicine.  To grow well, microalgae need enough nutrition. Another alternative use of the algae is for organic fertilizer. The fertilizer product can increase the income of the fishers, since it can change the useless into usefull things.  In the first year, we produced fertilizer in the laboratory and natural food such as microalgae powder. In this research we used an experimental method with Splitplot Design and 3 replications for each treatment of combination of aquatic weeds by giving different concentration. The research result indicates that population growth of microalgae cells is sigmoid with a peak population at the sixth day after inoculation and Salvinia natans with extract concentration of 900 ppm showed the best production for biomass cell of  Spirulina platensis.
Penambahan Ekstrak Alga Sargassum duplicatum Bory pada Medium Kultur In Vitro terhadap Pertumbuhan seedling Anggrek Vanda tricolor Lindl. Eko Adiguna; Murni Dwiati; Kamsinah Kamsinah
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 35, No 1 (2018)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2018.35.1.595

Abstract

Vanda tricolor is an orchid species that has many advantages, some of which are due to its beautiful and various flowers of unique shape and size. Propagation of V. tricolor can be performed by in vitro culture.In vitro culture technique needs appropriate medium to support orchid growth. An algae species, Sargassum duplicatum, can be added into the medium, since it contains growth regulating substances, such as auxin and gibberellin, which are useful for seedling growth. This study aims to know the effect of S. duplicatum application into in vitro culture medium of V. tricolor on the seedling growth and to know the best application of the algaein increasing seedling growth. An experimental method was arranged in a Completely Randomized Design (CRD). The treatments were S. duplicatum weight of 0, 12, 24, 36, 48 and 60 g/L replicated three times respectively. The variable examined was seedling growth with parameters comprising day of new leaf emergence, leaf number, leaf length, day of new root emergence, root number, root length and plant height. Data were analyzed using ANOVA or F test with confidence level of 95% and 99%. LSD was carried out when F test showed significant difference. The results reveal that application of S. duplicatum extract has significant effect on the growth of V. tricolor seedling. The best application is of 36 g/L algae weight.
Sebaran dan Zonasi Krustasea Mangrove di Delta Mahakam, Kalimantan Timur Rianta Pratiwi
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 23, No 3 (2006)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2006.23.3.171

Abstract

A study on the distribution and zonation of mangrove crustacean in Mahakam Delta, East Kalimantan was conducted in July 2004. The three research locations were selected i.e. Muara Ilu, Muara Kaeli, Muara Bayor and Muara Beji. Crustaceans were collected by applying quandran methods (1 x 1 m) in transect lines. The diversity index (H’) of crustacean in Delta Mahakam: 1.382 - 2.028 and the Eveness  index (E) : 0.5877 - 0.7101. The highest diversity index was recorded from muara Kaeli and the highest Evenes index  was in Muara Ilu.
Jenis Kelelawar Pemakan Buah (Pteropodidae) di Taman Wisata Alam Gunung Meja Manokwari Freddy Pattiselanno; Petrus I. Bumbut
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 28, No 2 (2011)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2011.28.2.263

Abstract

A study of bats was aimed to collect information on the presence of fruit bats that predicted involved in pollination or seed dispersal of certain commercial fruit plants within and around the Taman Wisata Alam Gunung Meja, Manokwari.  Forty five individual consists of 8 (eight) species: Dobsonia minor, Dobsonia moluccensis, Macroglossus minimus, Nyctimene aello, Nyctimene albiventer, Nyctimene draconilla, Rousettus amlexicaudatus and Syconicteris australis have been captured and identified during the survey.  It was assumed based on observation of vegetation found around the study site that the presence of fruit bats species help to maintain and contribute to seed dispersal of the fruit plants.  Further study is required to obtain accurate data through the stomach content collection of the observed bats.  Land conversion and illegal hunting was frightened influence the bat habitat and population which finally impact to fruit production.
Indeks Gonadosomatik dan Struktur Histologis Gonad Ikan Uceng (Nemacheilus fasciatus, Valenciennes in Cuvier and Valenciennes, 1846) Luthfi Nurhidayat; Febrina Nanda Arfiani; Bambang Retnoaji
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 34, No 2 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2017.34.2.456

Abstract

Uceng fish is wild native fish of Indonesia that live in fresh water river. It is very common that people consume the fish for it high protein contain. High demand of fish availability cause the fish exploitation increasing rapidly in nature. The high intensity of fish exploitation, soon will induce declining population of the fish and leading to extinction. Uceng fish cultivation is one alternative to solve the tread. The purpose of this research is to examine the Gonadosomatic Index (GSI) and gonad histological structure of male and female uceng fish (Nemacheilus fasciatus) on the period of immature and mature. Fish were dissected , weighed and the gonads were measured for gonadosomatic index. Moreover, gonad were fixed in NBF 5% for histological preparation by paraffin method and which than were stained by Hematoxylin-Eosin. The result were analyzed for both qualitatively and quantitatively. Qualitative analysis was conducted for gonad histological structure of male and female fish, while quantitative analysis was for Gonadosomatic Index (GSI) of fish respectively. The result showed that there were differences on the gonadosomatic index of males and females fish, the histological structure of ovarian follicle and the composition of the spermatogenic cells of immature gonads and mature gonads of the fish respectively. It was conclude that there are differences on Gonadosomatic Index and gonad histological structure on immature and mature of male and female of Uceng fish (N. fasciatus).
Pengaruh Bakteri Pelarut Fosfat Dan Bakteri Penambat Nitrogen terhadap Pertumbuhan Tanaman Tomat pada Tanah Masam Yenni Widi Astuti; Lestanto Unggul Widodo; Iman Budisantoso
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 30, No 3 (2013)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2013.30.3.138

Abstract

Acid soil is the dried soil which has great potential for growing of agriculture commodity but this kind of soil has not been used optimally. The acid soil which has acidity reaction at pH<5,0 has Al poisonous potency, less of organic material and sensitivity toward erosion, and also less of nitrogen (N) and phosphor (P). Those problems can be solved by doing phosphate-solubilizing bacteria and nitrogen fixing bacteria inoculation. The aims of this study were to know the effect of phosphate-solubilizing bacteria, nitrogen fixing bacteria, and the mixture of both of them to the wet weight, height, level of nitrogen and phosphor of tomato plants. This research was conducted experimentally for six weeks by using completely randomized design (CRD) with 4 treatments. One treatment is a treatment without bacteria inoculation and the other treatments used bacteria inoculations which were phosphate-solubilizing bacteria inoculation, nitrogen fixing bacteria inoculation, and the mixture of both of them inoculations. Data were analyzed by using analysis of variance (ANOVA) at the 95% and 99% confidence level. The result proved the mixture of phosphate-solubilizing bacteria and nitrogen fixing bacteria is the most effective inoculation to improve the wet weight, height, level of nitrogen, and level of phosphor of tomato plant in acid soil.
Aktivitas dan Karakter Kitinase Isolat Trichoderma sp. W34 A4 Asal Kepulauan Raja Ampat Papua Barat Nunuk Widhyastuti; Muhammad Ilyas
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 29, No 1 (2012)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2012.29.1.228

Abstract

The study of chitinase activity was conducted on 13 selected fungal isolates Trichoderma sp. from Raja Ampat, West Papua and Mount Bromo, East Java. The result showed that fungal isolate Trichoderma sp. W34 A4 isolated from Raja Ampat has the highest chitinase activity (14.3 x10-3 U/ml). The highest chitinase was produced by Trichoderma sp. W34 A4 grown on the media containing colloidal chitin (1%), polypepton (0.1%), KH2PO4 (0.1%) and MgSO4.7H2O (0.05%) for 2 days of incubation. Those isolates produced high chitinase with initial pH range 3.0-8.0 and levels of colloidal chitin between 0.2% -2.0%. Chitinase of Trichoderma sp. W34 A4 has optimal activity at pH 5.0 and in temperature 45 °C. Chitinase from those isolate stable at a wide pH range but it is not stable to the high temperatures.