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Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal
ISSN : 08531629     EISSN : 08531629     DOI : -
Majalah Ilmiah Biologi Biosfera : A Scientific Journal merupakan peer reviewed jurnal yang diterbitkan oleh Fakultas Biologi Universitas Jenderal Soedirman. Media ini mewadahi hasil-hasil penelitian di bidang biologi tropika yang terbit tiga kali setahun (Januari, Mei, September).
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Articles 342 Documents
Biosorpsi Krom pada Air Lindi Menggunakan Biomasa Sargassum cinereum Sri Lestari; Slamet Santoso; Dwi Sunu Windyartini
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 28, No 2 (2011)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2011.28.2.268

Abstract

Leachate is liquid resulted from decomposition of organic waste that contains dissolved and suspended materials as product of microorganism degradation. Leachate comprises high concentration of organic material and heayy metal. High concentration of heavy metal in leachate can be optimally reduced without decreasing organic material concentration through biosorption process. Biosorption is metal binding through adsorption using inactive or dead organism. This research is aimed to perceive the number of biomass, contact duration and combination of the number of biomass and contact duration to adsorb chrome in leachate. Research method employed in this research is experimental method arranged in accordance with Complete Random Design with Split Plot Design. The treatment experimented in this research is contact duration of Sargassum cinereum with leachate as main plot (1 hour, 2 hours and 3 hours) and biomass S. cinereum as sub-plot (200 mg, 300 mg and 400 mg). Data is analyzed using range analysis (ANOVA) and followed by Test of Straight Actual Difference. The result shows that biomass S. cinereum is able to adsorp chrome in leachate. The number of chrome adsorbed in each treatment is different, depend on the contact duration and the number of biomass of S. cinereum.Three hours is optimum contact duration in adsorbing Cr up to 46,275%, meanwhile 400 mg of biomass S. cinereum is optimum concentration to adsorp Cr up to 46,536%. Combination of 3 hours contact duration and 400 mg of biomass S. cinereum could adsorb Cr up to 51,249%.
Aktivitas Immunodulator Ekstrak Etanol Umbi Bawang Merah (Allium cepa L.) terhadap Respon Imun Non Spesifik pada Mencit Jantan Galur BALB/C dengan Metode Carbon Clearance Diska Anggraeni Alfitasari; Anjar Mahardian Kusuma; Zainur Rahman Hakim
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 34, No 2 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2017.34.2.482

Abstract

The content of flavonoids on onion useful to maintain endurance. This research aims to know the activity of exstract immunodulator of onion bulb ethanol (Allium cepa L) on phagocytic activity in male mice balb/c with varian dose and campored immunodulator activity with positive control. This study is an experimental reseach with Completely Randomized Design (CRD). Preparation of extract ethanol onion roots done by maceration using 96% ethanol for 9 hours. The test performed on samples which consist of 25 mice with carbon clearance method, devided into 5 groups, which are : K – (without treatment), k+ (with imboos 13,3 ml/Kg Bw), P1 (dose 12%), P2 (dose 24%), and P3 (dose 48%). The test performed for 5 days, and on the 7th day injected carbon (pelicans) as much 0,1 ml/ 10 gr Weight intravenousty, their blood taken before carbon injection (minute 0) and minute 4, 8, 12, and 16. After their transmittance measure using spectrophotometer at 600,5 nm wavelength. This research shows that extract ethanol of onion balb has immunodulator activity effect on non-specific immune response on male mice balb/c with carbon clearance method. The dose of 12% (v/v), 24% (v/v) and 48% (v/v) dose have lower immunostimulatory capacity than positive control.
Pengaruh Jenis dan Konsentrasi Bahan Nabati terhadap Perkembangan Aspergillus flavus pada Medium PDA dan Biji Kacang Tanah Sumartini Sumartini; Eriyanto Yusnawan
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 22, No 1 (2005)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2005.22.1.85

Abstract

An experiment aimed to obtain botanical fungicide and its concentration to inhibit the growth of Aspergillus flavus was done in the mycology laboratory, Indonesia Legume and Tuber Crops Research Institute, Malang on June–August 2003. Kancil and Mahesa varieties of groundnut,  A. flavus  fungus, garlic, onion and ginger were used in this experiment.  The effectiveness of botanical fungicide extracts to inhibit A. flavus on PDA was done by culturing A. flavus (5 mm diameter) in the petridish ( θ = 9 cm). This experiment was arranged in a factorial completely randomized design with 5 replications. Factor A was botanical fungicide extracts (garlic, onion, ginger, and sterile water) and factor B was botanical fungicide concentrations (10%, 15%, 20%, dan 25%). The level concentration of botanical fungicide extracts  were poured into Petridish containing PDA medium before culturing A. flavus. The growth diameter of A. flavus was measured as a parameter. A similar experiment for testing  the effectiveness of botanical fungicide extracts to inhibit A. flavus was also done on groundnut kernel.  It was arranged in completely randomized design factorial, 3 replications. Fifty groundnut kernels were dipped on botanical fungicide extract for 3 minutes, placed on plastic tray (30 x 24 x 5 cm3) and layered with wet tissue sterile before used. After botanical fungicide extracts on kernels were air dried, the kernels were inoculated with spore suspension (approximately 104 spore/ml). Disease intensity of A. flavus was measured as a parameter. The result showed that the garlic extract treatment was more effective than those of onion extract, ginger extract and without botanical fungicide. The growth rate of  colony diameter of  A. flavus on PDA medium were 0.15 cm, 3.88 cm, 2.59 cm, and 4.30 cm respectively. The used of garlic extract at a concentration 10% on PDA could inhibit colony diameter growth of A. flavus by 97% as compared to without botanical fungicide extract. The garlic extract treatment, onion extract, ginger extract and without botanical fungicide extract showed that disease intensity A. flavus on groundnut kernels were 13.21%, 22.00%, 20.74%, and 25.18% respectively. An effective concentration to inhibit A. flavus infection on groundnut kernels was achieved by using garlic extract 20%. The use of its extract could reduce 73% of disease intensity. This result was different from the treatment on PDA. This was due to the ability of A. flavus to degrade cell walls. This process takes time meanwhile much of botanical fungicides extract have evapoured.
Isyarat Sentuhan Antena Semut (Dolichoderus Sp) Pada Tubuh Aphid (Aphis Sp) Untuk Memperoleh Embun Madu Yayan Sunjaya; Harry Buana; Mimi Halimah
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 26, No 3 (2009)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (235.246 KB) | DOI: 10.20884/1.mib.2009.26.3.183

Abstract

Ant, Dolichoderus sp, interact mutually with aphid (Aphis sp).  The ants specifically communicate with the aphid by means of antennal sign.  A hungry ant will approach an aphid and touch it dorsal part using ant antennae; in respond to this, will expel honey dew.  To further study the pattern of communication between the ant and the aphid, their movement was recorded using Handycam JVC GR-SXM 300 AC.  The records were processed using Adobe Premier Pro 1.5.  The results showed that the ant antennae moved in a specific pattern which could be classified into 4 main steps.     The first step consisted of 8 movements which formed 2 movement patterns.  The second step consisted of 3 main repeated movements.  The third step consisted of 3 main repeated movements.  The fourth step consisted of 6 main movements which were repeated several times before the final movement.  Such pattern of antennae movement was recognized by the aphid as tactile signal of ant identity.
Pemanfaatan Ekstrak Daun Nimba (Azadirachta indica A. Juss) untuk Pengendalian Penyakit Layu Fusarium pada Tanaman Tomat (Lycopersicum esculentum Mill.) Juni Safitri Muljowati; Aris Mumpuni
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 24, No 2 (2007)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2007.24.2.275

Abstract

The aim of this study was to find out the effect of neem leaf extract (Azadirachta indica A. Juss.) on the growth medium of tomato plant toward fusarial wilt and to determine effective concentration and time of application of the extract to control the wilt. Experiments arranged in a Completely Randomized Design (CRD) was employed for in vitro antimicrobial assay and in a Randomized Complete Block Design (RCBD) for in planta antimicrobial assay, both of which were to find out the inhibitory effect of the extract on colony growth of Fusarium oxysporum (Schlecht). f.sp. lycopersici (Sacc.) Snyd. et Hans. (Fol).  The results indicated that neem leaf extract concentration of 5% applied at 7 DAP (Day After Planting) was the most effective treatment in inhibiting disease intensity of fusarial wilt on tomato plant showing inhibitory effect of up to 89.32%.
Keanekaragaman dan Potensi Lobster (Malacostraca: Palinuridae) di Pantai Pameungpeuk, Garut Selatan, Jawa Barat Rianta Pratiwi
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 35, No 1 (2018)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2018.35.1.524

Abstract

Coral shrimp (Panulirus spp.) or known as lobster is a potential fishery commodity and economic value. The demand for domestic and export market continues to increase both as local consumption and export. Indonesia is the largest lobster fishery producer in almost all Indonesian waters, from the west coast of Sumatra to the east coast Jayapura, one of which is Pameungpeuk beach, South Garut, West Java. As a result of increasing demand, fishermen try to catch as many lobsters and the impact of lobster prices also in creases and whereas fishermen continuously take it from nature. This study was conducted to determine the diversity and potential of lobster species especially living in high wavy waters along the southern coast of Garut (Pamengpeuk), West Java.The results of observation are only three types of crayfish, namely: a. Panulirus homarus (green lobster sand); b. Panulirus longipes (lobster flower/ red lobster) and Panulirus versicolor (green lobster/bamboo); is most commonly found in the area of south Garut, Pameungpeuk beach with high and strong waves. The percentage of female P longipes species (42.88%) was higher compared to male P. homarus (40%) and ovigerous female of P. versicolor (37.14%). While the sexual ratio between P. homarus (1:2.0:2.0); P. longipes (1:1.2:1.0) and P. versicolor: 1:1.2:1.2  which statistically showed significantly different p <0.05.
Pengaruh iradiasi ultraviolet (254 nm) terhadap pelemahan kemampuan menginfeksi mirasidium Fasciola gigantica Edy Riwidiharso; Billalodin Billalodin
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 23, No 3 (2006)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2006.23.3.167

Abstract

The controlling of fascioliasis has not been satisfying so far, since the effort of breaking off the fluke life cycle often leads to resistance. The resistance is due to the synchronization between the parasites and their snail host and high infection ability of the parasites enabling them to finish their life cycle. Ultraviolet irradiation provides alternative approach possibly applied to cope with the resistance. The objectives of this research were to know the ability of F. gigantica miracidium in infecting Lymnaea sp. at various ages and  to determine time exposure of ultraviolet (254 nm) effectively reducing the ability of miracidium infection. The results indicated that susceptible age of Lymnea javanica is five weeks old, while the most effective time exposure of ultraviolet (254 nm) in weakening  the ability of Fasciola gigantica miracidium infection to L. javanica is 60 seconds (P < 0,05).  At the moment the study on reinfection effect of F. gigantica miracidium on the immunity of L. javanica is still in progress.
Kajian Etnobotani Pisang-pisang Liar (Musa spp.) Di Malinau, Kalimantan Timur Lulut D. Sulistyaningsih; Albert H. Wawo
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 28, No 1 (2011)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2011.28.1.259

Abstract

Ethnobotany studies of wild banana species in Malinau, East Kalimantan has been done. There are two varieties of wild banana species in Malinau, e.g. Musa borneensis Becc. var. flavida Häkkinen & Meekiong and Musa acuminata Colla var. microcarpa Nasution. They have an important roles in the customs of the local communities in Malinau, Punan tribes as land certificate. In addition, young pseudostem of M. borneensis Becc. var. flavida often used as food in the traditional parties. The description and the uses of M. borneensis var. flavida and M. acuminata var. microcarpa are presented here.
Efektivitas Bacillus thuringiensis dalam Pengendalian Larva Nyamuk Anopheles sp. Citra Inneke Wibowo
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 34, No 1 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2017.34.1.469

Abstract

Nyamuk Anopheles sp adalah vektor penyakit malaria. Pengendalian vektor penyakit malaria dapat dilakukan secara biologis yaitu dengan menggunakan Bacillus thuringiensis. Tujuan penelitian adalah untuk mengetahui efektivitas konsentrasi Bacillus thuringiensis dalam pengendalian larva nyamuk Anopheles sp.Penelitian ini dilakukan secara eksperimental menggunakan Rancangan Acak Lengkap Faktorial (RAL Faktorial) yang terdiri atas dua faktor yaitu konsentrasi Bacillus thuringiensis dan stadia larva Anopheles dengan pengulangan tiga kali.Perlakuan yang dicobakan adalahkonsentrasi Bacillus thuringiensis (A) yang terdiri atas 5 taraf:A0: konsentrasi B.thuringiensis 0 CFU.mL-1, A1: konsentrasi B.thuringiensis 102 CFU.mL-1, A2: konsentrasi B.thuringiensis 104 CFU.mL-1, A3: konsentrasi B.thuringiensis 106CFU.mL-1, A4: konsentrasi B.thuringiensis 108CFU.mL-1. Perlakuan tahapan instar larva Anopheles sp. (B) adalah sebagai berikut:B1: stadia larva instar I, B2: stadia larva instar II, B3: stadia larva instar III, B4: stadia larva instar IVsehingga terdapat 60 satuan percobaan. Hasil penelitian  menunjukkan konsentrasi B. thuringiensis isolat CK dan IPB CC yang paling berpengaruh dalam pengendalian larva Anopheles sp adalah 108 CFU.mL-1 . Instar larva yang paling peka terhadap B. thuringiensis isolat IPB CC adalah instar I dan II sedangkan instar yang peka terhadap isolat CK adalah instar II, Perlakuan konsentrasi isolat B. thuringiensis dan tingkat instar larva yang paling baik dalam pengendalian larva Anopheles sp. adalah 108 CFU.mL-1, dan instar I dan II.
Variasi Genetik Spesies Mangrove Ceriops tagal berdasarkan Marka RAPD Dhuta Sukmarani
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 30, No 3 (2013)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2013.30.3.134

Abstract

Ceriops tagal are found at Segara Anakan Cilacap, Baluran National Park and Karimunjawa National Park. Information on the genetic diversity can be used for conservation policy. Genetic variation of C. tagal were analysed by using Random Amplified Polymorphic DNA (RAPD). This study aims to determine the genetic variation in each population and between populations C. tagal based on RAPD markers. The total DNA was extracted with CTAB extraction method. Amplification was performed using 10 primer (OPA-3, OPA-10, OPB-1, OPB-6, OPB-7, OPB-8, OPB-10, OPB-12, OPB-17 and OPB-18).  Amplification includes pradenaturation at 94°C for 5 min, followed by 45 cycles of denaturation at 94°C for 1 min, primer annealing at 37°C for 1 min, and primer elongation on 72°C for 1 min 30 sec, final extension at 72°C for 5 min and ends with holding at 4°C for 1 min. The analysis was done by changing the pattern of fragments to binary data (for bands appear = "1" and for bands do not appear = "0"). It is done using a program GenAlEx 2.1 and NTSYS-PC 6.1. A population that has the highest genetic variation (h) was Segara Anakan (0.308), followed by Baluran (0.295) and Karimunjawa (0.123). Baluran has the highest polymorphism (75.00%), Segara Anakan 69.23%, while Karimunjawa was 30.77%. The results of AMOVA, showed that the percentage of genetic variation within populations is greater (77%) than among populations (23%). The results of PCA analysis showed a similar pattern of genetic distance and UPGMA. Samples of Karimunjawa clustered with samples of the population, SA1 and SA3 likely close to the sample of Karimunjawa and SA2 are likely not one group with another sample of the Segara Anakan. Results of mantel test, the value of r = 0.347 and p = 0.02.