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Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal
Published by Universitas Jenderal Soedirman
ISSN : 08531629     EISSN : 08531629     DOI : -
Core Subject : Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science
Majalah Ilmiah Biologi Biosfera : A Scientific Journal merupakan peer reviewed jurnal yang diterbitkan oleh Fakultas Biologi Universitas Jenderal Soedirman. Media ini mewadahi hasil-hasil penelitian di bidang biologi tropika yang terbit tiga kali setahun (Januari, Mei, September).
Arjuna Subject : -
Articles 342 Documents
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Pengaruh Inokulasi Jamur Mikoriza Arbuskular Terhadap Penyakit Busuk Pelepah Pada Jagung Di Lapangan Soenartiningsih Soenartiningsih; Ambarwati Tj. Harsoyo; Nursamsi Pusposenjoyo; John Baco Baon
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 23, No 2 (2006)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2006.23.2.161

Abstract

Sheath blight caused by Rhizoctonia solani is one of the important diseases in corn. A study on the effect of arbuscular  mycorrhizal  fungi in controlling  sheath blight disease was conducted at pilot project scale in Jambangan Village, Malang District. Three-replicated treatments were arranged in a split-split plot design. The results   showed that highest attack intensity occured at treatments without inoculation of  arbuscular mycorrhizal fungi. The mean of attack intensity for Wisanggeni variety  was 70,05%, while that for GM 30 line was 73,79%. The attack intensity at Wisanggeni variety inoculated with arbuscular mycorrhizal fungi was 45.39% to 49.46%, while that at GM 30 line was 47.52% to 56.12%. The infection of sheath  blight disease reduced the production in terms of knob part and seed  weight even  though the plant was inoculated with arbuscular mycorrhizal fungi of Glomus sp. or  Acaulospora mellea. The reduction of knob weight at Wisanggeni variety was 14.2% to 15.0% and that of seed weight was 21.4% to 23,0%.  At GM 30 line the reduction of knob part was 12.0% to 17.2%, while that of seed weight was 19.1% to 20.4%.
Studi Agronomis Tanaman Padi (Oryza sativa L.) Hasil Ko-Kultivasi Beberapa Strain Agrobacterium tumefaciens Pupita Deswina; Inez H. Slamet Loedin
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 28, No 1 (2011)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2011.28.1.254

Abstract

The transgenic rice of Cisadane and Rajalele cultivars (cv) was obtained by co-cultivation of rice callus by 4 strain Agrobacterium tumefaciens containing 4 different binary vector (pIG 121 Hm, pCAMBIA 1301, pTOK 233 and pMOG22 BarGus). The transgenic plantlets were acclimatized and grown in the growth chamber to investigate their agronomic characteristic in comparison to control plants. The results showed that there was no difference in the number of spikelet and seeds/spikelet among different transgenic lines co-cultivated by different strains. The number of seed/spikelet of transgenic rice cv. Rojolele and Cisadane compared to their control did not show significant difference. However, the total number of spikelet was different only for cv. Rojolele.
Studi Tentang Ekologi dan Habitat Planaria, Sp. di Subang: Kelimpahan dan Biomassa Merupakan Indikator Kualitas Air Bersih Hertien Koosbandiah Surtikanti; Ulfah Bahabazi
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 30, No 2 (2013)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2013.30.2.128

Abstract

Planaria sp. in Bukit Tunggul (Lembang) is rare within 10 years. This animal is sensitive to polluted water. Beside that, the number of this animal is decreasing due to alteration of landuse and climate change. This animal plays an important role as a bioindicator for clean water and as a media in learning of biology in high school as well as in university. Due to these need, study on ecology and distribution of Planaria sp. is done in Panaruban water fall, Subang. The sampling location is limited only at Cimuja, Karembong, Sawer and Goa Badak, which have an environment characteristics variation. Observations are done including water quality chemically, land use, and climatic factors within 3 times monthly. Planaria sp. counting is done stratified random sampling using a 1x1 m quadran. The number and biomass of Planaria sp. related to climatic factors are discussed. In general, it can be concluded that, abiotic environmental factors contributes to the abundance and biomass of Planaria sp.
Efek Antimitosis Biomutagen dari Tanaman Kembang Sungsang (Gloriosa superba L.) pada Pembelahan Sel Ujung Akar Kecambah Cabai Merah Besar (Capsicum annuum L.) Eti Ernawiati; Sri Wahyuningsih; Yulianty Yulianty
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 31, No 2 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2014.31.2.214

Abstract

Flame lily (Gloriosa superba L.)  is one of the plants containing colchicine, often used as antimitotic compound, in the whole organ. This study aims to gain the combination of extract source  and extract concentration of G. superba capable of inhibit mitosis in root tip cells of chili  sprouts.  The plant is extracted  using the extraction and dilution methods to determine the extract concentration of the treatment. The squash method is used to make a  mitosis preparation. The study is arranged in a factorial using Randomized Complete Block Design (RCBD) with 4 replications. The first factor is extract source:   roots, stems, and leaves. The second factor is the extract concentration: 0, 20.40, 60, and 80%.   The observation shows that the extract of leaf can inhibit mitosis more than exctract of bulbs and roots.  All of the concentration treatment from all extract sources tend to result in similar mitotic index, but significantly different from cells that are not given treatment. The combination of the source extract and concentration of the extract result in varied mitotic index, but  it is obvious that the leaf extract with a concentration of 80% gives the most inhibiting mitosis index: 1.298%.
Uji Antagonisme Lentinus cladopus LC4 terhadap Ganoderma boninense Penyebab Penyakit Busuk Pangkal Batang Kelapa Sawit Erni Angraini
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 34, No 3 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2017.34.3.512

Abstract

The growth of oil palm can not be separated from disease attack. One of the diseases that attack is the Base Stem Rot disease caused by Ganoderma boninense. Efforts to control the pest and disease one of them by using Lentinus Cladopus LC4 biological controller so that the purpose of this study is to determine the potential of L.cladopus LC4 as biological controlling agent G. boninense. The method used is L. cladopus LC4 antagonism test against G. boninense. The results showed that L. cladopus LC4 had antagonistic potency against G. boninense pathogen, although the mechanism did not show any inhibition zone. Therefore, L.cladopus LC4 may be considered for the prevention and control of plant diseases in the field.
Pengujian Skala Karamba Jaring Apung Benih F2 Lukas (Puntius bramoides) Produk Pradomestikasi (I): Kualitas Telur, Sintasan Benih dan Kemampuan Memijah Priyo Susatyo; Sugiharto Sugiharto
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 31, No 3 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2014.31.3.306

Abstract

Pre domestication research has been done on fish catches from the Serayu river,  Banyumas, lukas fish (Puntius bramoides) and brek fish (P. orphoides) in natural ponds. The parents as pre domestication product is already successfully demonstrated the ability of adaptive in the new environment in terms of aspects of natural food, has been known to their periodically hormonal profiles, profiles of gametogenesis and spawning capabilities in the new environment (natural pond). Further research aims to determine : (1) The degree of eggs hatching of lukas, (2) survival rate of  F2 seeds and (3) the degree of mortality in the floating net on Serayu river. (4) how long it will take and how the ability of the  post spawning F1 parents to be able to spawn again. Research using survey method. Some units of floating net used for maintenance, testing spawning and growth of parents and seeds of lukas. The results : (1) The degree of eggs hatching between 51%-89%; (2) degree of viability of seeds in floating net  is 54%-92%; (3) while the mortality rate of  seeds 8%-48% and (4) the length of time it takes  male and female parents to reach the next spawning period is ± 6 months. The F1 parents spawn after 6 months on a scale of cages.
Penelusuran Aktivitas Antibakteri dari Kulit Akar Tumbuhan Medang Seluang (Litsea spathulata) terhadap Bakteri Uji Escherichia coli dan Shigella dysentriae Munawar Munawar; Elfita Elfita
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 24, No 1 (2007)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2007.24.1.152

Abstract

Screening for antibacterial activity from root bark of Litsea spathulata had been done. Antibacterial test on each fraction showed that hexane had the highest activity followed by ethyl acetate and methanol. Major compound from antibacterial active fraction had been isolated. Identification of this compound was carried out applying phytochemistry assay and spectroscopic methods, i.e. ultraviolet (UV), infrared (IR), and Gas Chromatography- Mass Spectroscopic (GC-MS). Minimum inhibiting concentration (MIC) value of the active fraction was 780 mg/mL to Escherichia coli and 860 mg/mL to Shigella dysentriae.
Penggunaan Ekstrak Rimpang Lengkuas untuk Mengendalikan Busuk Leher Akar pada Tanaman Terong (Solanum Melongena L.) Juni Safitri Muljowati; Eddy Tri Sucianto
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 29, No 2 (2012)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2012.29.2.243

Abstract

A research to find antifungal activity of galanga rhizome  (Alpinia galanga L.) on Sclerotium rolfsii  as the causal agent of white mold and stem rot disease on eggplant has been done.  The galanga extract was obtained by extracting the rhizome using ethanol solvent.  The experiment design was Completely Randomized Design (CRD) in a factorial pattern, involving two factors, i.e. the rhizome extract concentration (E) with six levels of 0%, 5%, 10%, 15%, 20%, and 25%; and application time (W) with two levels of  three days interval and seven days interval.  The result showed that the best extract concentration was 20% that was applied in three days interval.
Karakterisasi Molekuler Ikan Gurami Soang (Osphronemus gouramy Lac.) yang Mati pada Rentang Waktu Berbeda Menggunakan PCR-RFLP Gen Major Histocompatibility Complex Kelas II B Jaka Tri Spetiawan; Agus Nuryanto; Hendro Pramono; Kusbiyanto Kusbiyanto; Petrus H. Tjahja Soedibja
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 33, No 2 (2016)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2016.33.2.373

Abstract

Gurami (Osphronemus gouramy Lac.) is a popular fish species among Indonesian people. Several Gurami strains have been cultivated by fish farmer, one of which is Gurami Soang. This strain is belived to have a faster growth rate compared to other strains. However, like other strains, the fingerling of Soang strain have also a low survival and suceptible to disease, especially that caused by Aeromonas hydrophila infection. It has been proved that seeds from a single spawning event show varibale disease resistance. The difference in resistance among individuals is suggested related to the difference in their genetic component. One of the genes responsible for resistance is Major Histocompatibility Complex (MHC) class II B gene. Variability in resistance can be analyzed by using PCR - RFLP technique. PCR-RFLP is a technique that can produce a specific DNA fragments by PCR, followed by cutting the PCR product using restriction enzymes to describe the presence or absence of restriction sites in DNA fragments. This research aims to determine genetic marker to differiantiate between resitant and irresistant individual of Gurami Soang infected by A. hydrophila which die at a different time priod based on PCR-RFLP MHC class IIB gene. The study used survey method with purposive random sampling. The Data of PCR-RFLP band patterns were analyzed descriptively. The result indicated that cutting of the MHC class II B gene using HinfI produce two RFLP bands with 300 bp and 100 bp length in all samples. Meanwhile, the MHC IIB gene was not cuted by PstI, HindIII, BamHI and EcoRI enzymes forall samples. These mean that MHC II gene in all individuals were monomorphic. Therefore,it can be concluded that there is no specific genetic marker to differentiate gurami soang individulas which was dying in different time periods.
Motilitas dan Viabilitas Spermatozoa Itik Lokal (Anas platyrhynchos) Setelah Penyimpanan Refrigerator dalam Ekstender Dikombinasi Berbagai Konsentrasi Krioprotektan Gliserol Arie Amelia; Dadang Mulyadi Saleh; Hendro Pramono; Yulia Sistina
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 30, No 1 (2013)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2013.30.1.116

Abstract

The economic value of ducks has increased one Day Old Duck in large quantities and short time, effects on artificial insemination technologies improvement. Spermatozoa used for artificial insemination, could be derived from sperm preservation biotechnology, by means to maintain or extend their viability and motility of those spermatozoa. Extenders and cryoprotectant are needed for maintaining sperm motility and viability during storage or preservation. This study aims to assess motility and viability of local ducks spermatozoa after six days srotrage in 3 different extenders (Ringer's lactate, Tris or PBS) in combination with 5 different concentrations (0, 2, 4, 6, and 8%) of cryoprotectant glycerol. Results showed that sperm motility and viability was very highly significant (P<0.01) different among treatments, extender factor as well as duration storage time also very highly significantly (P<0.01) affected motility and viability of treated sperm. However, glycerol concentration factor was not significant (P>0.05) affected motility and viability of the sperm. The best treatment, resulted in the highest percentage motility and viability of sperm with progressivity score >1, was the 8% glycerol in PBS storage for up to 2 days (75.00 ± 21.79% and 71.67±14.43%). This protocol could be applied in artificial insemination trial.

Page 16 of 35 | Total Record : 342

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