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Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal
ISSN : 08531629     EISSN : 08531629     DOI : -
Majalah Ilmiah Biologi Biosfera : A Scientific Journal merupakan peer reviewed jurnal yang diterbitkan oleh Fakultas Biologi Universitas Jenderal Soedirman. Media ini mewadahi hasil-hasil penelitian di bidang biologi tropika yang terbit tiga kali setahun (Januari, Mei, September).
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Articles 342 Documents
N-Acyl Homoserine Lactones sebagai Signal Quorum Sensing untuk Meningkatkan Efektifitas Bakteri Fosfat Tamad Tamad
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 31, No 1 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2014.31.1.220

Abstract

Phosphate bacteria (PB) are able to release P-adsorption by soil. PB  effectiveness in releasing adsorption P  controled by quorum sensing (QS) signal. PB  produces a QS signal  as N-acyl homoserine lactones (N-HSL). The aim of this study are to determine the type of N-HSL as QS signal of PB (Pseudomonas trivialis, P. putida and P. fluorescens) and find the source of N-HSL from root extracts of some plants (rice, corn, bamboo, banana and peanuts). Analysis of N-HSL using HPLC (Hitachi UV-VIS detector L-2420), L-2200 autosampler (20 mL), L-2130 pump and column C OOF-4250-CO/10 µm LaChrom Ultra 18 (2 µm) 100 A 150 x 4.60 mm 10 m KPOW 490065-1 (Phenomenex), temperature 60° C, flow rate of 0.9 mL/minute  and a gradient of 30-100 % in 1.0 minutes. Standard N-HSL  is C4-HSL, 6, 8, 10, 12 homoserine lactones (Sigma-Aldrich, Germany) was dissolved in acetonitrile (Merch, India) with a concentration of 50 mM. P-dissolved by PB determined by staining NVM and a spectrophotometer at a wavelength of 413 nm. PB population is determined by the OD (optical density) at a wavelength of 600 nm. PB  populations  on medium Pikovskaya influenced by PB isolates, the type of P sources and duration of incubation. N-HSL generated by PB highest is Butanoyl (C4) homoserine lactones. PB isolates 9 and Ca-phosphate sources produce N-HSL most. Root extract of rice, corn, bamboo, bananas and peanuts can be a source of   N-HSL. Soluble phosphorus from medium Pikovskaya influenced by the type of PB isolates and source of P.
Uji Antagonis Jamur Tempe (Rhizopus Sp) terhadap Bakteri Patogen Enterik Dewi Peti Virgianti
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 32, No 3 (2015)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2015.32.3.339

Abstract

Indonesian tempeh is a food product made from soybeans fermented by Rhizopus oligosporus. The role of R.oligosporus as the primary fungi in soybean is very important, that changing the composition of the substrate soybeans into food that is more nutritious and contains many enzymes and bioactive compounds, including antibacterial compounds. This study was aimed at testing the antibacterial activity of Rhizopus sp fungus isolated from commercial tempeh against several enteric pathogen bacteria Escherichia coli ATCC 25922, Salmonella typhimurium ATCC 14028 and Shigella flexneri ATCC 12022. The tests were conducted by the antagonism test agar diffusion method, by putting pieces of SDA that has grown Rhizopus sp on agar Muller Hinton which test bacteria was inoculated. The incubation period was 24 hours at 37 ° C. The results showed that Rhizopus sp has antagonistic to the bacteria, which produce inhibitory zone of 28 mm against the E.coli ATCC 25922, 26 mm against S.typhimurium ATCC 14028 and 39.5 mm against Shigella flexneri ATCC 12022. Based on these results it can be concluded that the Rhizopus sp isolated from tempeh is antagonistic against enteric pathogenic bacteria. The results of this study reinforce the benefits of tempeh as a functional food.
Produksi Efipium Dafnia (Daphnia carinata King) dengan Pengaturan Fotoperiode dan Kepadatan Kultur Irsyaphiani Insan; Chumaidi Chumaidi
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 23, No 2 (2006)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2006.23.2.157

Abstract

Daphnia carinata King is a planktonic crustacean commonly used as natural food for freshwater fish. This experiment was conducted to evaluate the effect of different culture densities of daphnids and photoperiod on the ephippia production. Daphnids of different densities, i.e. 15, 20, and 25 individuals per 20 ml water media were cultured in small bottles.  The bottles were placed in a refrigerator and treated with two different photoperiods, i.e. 10.5 light hours : 13.5 dark hours (10.5:13.5) and 4 light hours : 20 dark hours (4:20).  All refrigerators were set at 12° C.  The experiment was arranged in a Complete Randomized Design with factorial treatments (3x2) replicated six times. The ephippia were colleted from every bottle for 45 days. The results showed that the photoperiod of 10.5: 13.5 and daphnid densities of 25 individuals per 20 ml produced most ephippia (12.1). Different culture density within the same photoperiod had no significant effect on ephippia production.
Studi Kasus Pemanfaatan Tumbuhan Sebagai Obat-Obatan Tradisional Oleh Masyarakat Adat Kampung Naga Di Kabupaten Tasikmalaya Nisa Nurmalasari; Sukarsa Sukarsa; Hexa Apriliana Hidayah
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 29, No 3 (2012)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2012.29.3.250

Abstract

A study entitled "A Case Study of Plant Utilization as Traditional Medicines by Indigenous Community of Kampung Naga" has been conducted from April-July 2010. The aims of the research to determine the type, how to used and to conserve plants used for medicine by the indegenous community in Kampung Naga. The research method was survey and the data collection was done with census method, interview and questionnaire for the tribe (kuncen), midwife (paraji), community leaders and residents of Kampung Naga.  The results of the research showed that there 108 plants species including to 50 families used as medicinal plants. The organ and nutritious plants used as medicine are the roots, rhizomes, tubers, stems, seeds, flowers, fruits, and leaves, or the whole plant parts. The way to use the medicinal plants includes boiing, just posted, and immediately eaten after steaming. The plants used as medicine were obtained from the gardens, forests, yards, roadside, moor and fields. The conservation efforts undertaken by the community of Kampung Naga consist of plant planting and cultural conservation.
Efek Ekstrak Daun Kembang Bulan (Tithonia diversifolia) terhadap Kadar Glukosa Darah Tikus Wistar (Rattus norvegicus) yang Diinduksi Alloxan Fitri Wening Sasmita; Eko Susetyarini; Husamah Husamah; Yuni Pantiwati
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 34, No 1 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2017.34.1.412

Abstract

Diabetes Mellitus merupakan penyakit metabolik yang ditandai kenaikan kadar gula darah atau hiperglikemia yang terjadi karena kelainan sekresi insulin atau kerja insulin. Alloxan menginduksi diabetes dengan merusak sel pankreas dan mengawali terjadinya hiperglikemia. Salah satu tumbuhan yang memiliki potensi obat dalam penurunan glukosa darah adalah daun Tithonia diversifolia yang mengandung senyawa flavonoid dan seskuiterpen. Tujuan penelitian ini adalah (1) menganalisis efek ekstrak daun kembang bulan terhadap kadar glukosa darah tikus wistar; dan (2) menentukan pemberian ekstrak daun kembang bulan yang memiliki pengaruh efektif dalam penurunan kadar glukosa darah tikus wistar. Jenis penelitian ini adalah eksperimental yang sesungguhnya secara in vivo yang dilakukan di laboratorium. Rancangan yang digunakan adalah True Experimental-Post Test Only Control Group Design mengacu pada Sambrook & Russel. Sampel yang digunakan adalah tikus wistar sebanyak 25 ekor yang terbagi dalam 5 kelompok perlakuan dan 5 kali pengulangan yaitu P1 (kontrol negatif), P2 (kontrol positif), P3 (dosis 1,28 ml/200g BB), P4 (dosis2,57 ml/200g BB), dan P5 (dosis 5,14 ml/200g BB). Teknik analisis data yang digunakan dalam penelitian ini adalah One-way ANOVA dilanjutkan uji Duncan. Hasil penelitian menunjukkan bahwa ada efek ekstrak daun kembang bulan terhadap kadar glukosa darah tikus wistar. Hal ini menunjukkan bahwa daun kembang bulan memiliki efek antidiabetes atau berperan sebagai antihiperglikemik pada pemberian dosis sebesar 5,14 ml/200g BB dengan rata-rata 136,8 mg/dl. 
Aktivitas Protease dan Kadar Protein Tubuh Ikan Lele Dumbo (Clarias gariepinus) pada Kondisi Puasa dan Pemberian Pakan Kembali Wieka Mahalida Hanum; Untung Susilo; Slamet Priyanto
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 30, No 1 (2013)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2013.30.1.121

Abstract

Three treatments and four replications in randomised completely design were conducted to reveal the protease activity and body protein content on catfish, Clarias gareipinus, under starvation and refeeding conditions. The result showed that the protease activity has no significant different (P>.05) in first week, but has significant different (P<.05) in second week after treatment.  Body protein content has significant different (P<.05) in first and second weeks after treatment. We concluded that the protease activity and body protein content decreased at deprivation, but increased after two weeks refeeding.
Tipe Echolokasi serta Struktur Larynx pada Miniopterus dan Rhinolophus Abdul Rachman; Johanes Sugiyanto; Luthfi Nurhidayat; Ardaning Nuriliani; Aswi A. Rofiqoh; Agus Hermawan; Roesma Narulita
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 31, No 3 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2014.31.3.209

Abstract

A bat is the only mamal with true flight ability and have capability to perform echolocation. Miniopterus  and Rhinolopus  are bat species that have some different morphological characteristics but both of them commonly live in the cave. The research were purposed to study the differences and connection of echolocation call type and larynx’s structure of Miniopterus and Rhinolopus  The two bat species were collected from 20th and 23th cave of Japanese Cave Complex, Kaliurang, Sleman, Yogyakarta. Specimens were taken to the laboratory for further analysis. Echolocation signals of both bat species were recorded using ultrasonic microphone and were analyzed with Batscan 9.0 software. Specimens were subjected for anatomical and histological structure analysis of the larynx. Anatomical structure observations were carried out with specimens dissection and they were fixed in 20% formol solution. Some samples of larynx of each species were processed and stained with Alizarin Red’s-Alcian Blue staining for larynx’s bones observation. Histological preparation of larynx was performed using paraffin method and stained with Hematoxylin-Eosin and MAF. The results showed that echolocation signals of Minipterus  have lower frequency (peak frequency between 44.1-51.6kHz) compared to that of Rhinolopus  (peak frequency between 54.4-64.1 kHz). However, Miniopterus  has better ability to modulate the signal frequency than Rhinolopus  Both species are able to produce a long-duration signal but Rhinolopus  can produce longer signal duration. These results can be related to the differences of larynx’s anatomical and histological structure of both species which are equally able to keep the intensity of echolocation signals but have different work mechanisms.
Kajian Kualitas Air Sungai Condong yang terkena Buangan Limbah Cair Industri Batik Trusmi Cirebon Pipin Supenah; Endang Widiastuti; Rawuh Edy Priyono
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 32, No 2 (2015)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2015.32.2.302

Abstract

The aims of this study were to assess the water quality of the River Condong and Condong River Quality Statebased on physical, chemical and biological parameters. Sampling was carried out in seven stations with 3 repetitions at intervals of one month. Water quality were analyzed descriptively by reference raw Water Quality Standard.  Determination of the stateof water quality using methods Storet. Results of laboratory tests showed that the water quality of the River Condong exceed the Water Quality Standard Group II based on Government Regulation No. 82 of 2001 and in particular textile waste quality standard TSS, DO, BOD5, COD, NH3, sulfide, Cr (VI), phenols, oils and fats, Biological parameters using makrobentos diversity index (H ') obtained a low value that is from 0 to 1.7077.  Quality state based Storet method, the value of  > -31. It can be concluded that the condition of physical, chemical and biological parameters, Condong River water showed waters not appropriate the Quality Standard for agricultural irrigation, freshwater fish breeding and infrastructure or water recreation facilities before and after the discharge of liquid waste of batik. Condong River quality state before and after the liquid waste of batik is heavily polluted.
Compensatory Growth and Feed Utilization of Humpback Grouper, Cromileptes altivelis Receiving Preset Period of Unfed-Fed Cycle Edy Yuwono; Purnama Sukardi
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 24, No 1 (2007)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2007.24.1.148

Abstract

The effect of preset periods of unfed-fed cycle on growth, feed efficiency and body condition indices of humpback grouper, Cromileptes altivelis was studied. The fish receiving non-feeding/feeding cycle of 1/2 day (T1); 1/2 and 3 days alternately (T2); 1/3 day (T3); were compared to that fed daily as a control (C). Each feeding treatment was assigned to five fiberglass tanks according to a completely randomized design. All fish was weighed to obtain final weight gain at the end of the experiment. Feeding rate (FR) was calculated during feeding period throughout the experiment. At the end of the experiment all fish was weighed to obtain final fish weight. Absolute growth rate (AGR); survival; consumption; cumulative feed consumption, and feed conversion efficiency (FCE) were calculated. Data were subjected to analysis of variance and Tukey test (P<0.05). The results showed that humpback grouper Cromileptes altivelis receiving preset unfed-fed cycle elicited compensatory growth and the fish showed hyperphagia and greater feed conversion efficiency.
Aktivitas Antifungi Ekstrak Etanol Daun Salam (Syzygium polyanthum (Wight) Walp.) terhadap Pertumbuhan Jamur Candida albicans secara in vitro Any Fitriani; Yanti Hamdiyati; Ria Engriyani Ria Engriyani
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 29, No 2 (2012)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2012.29.2.238

Abstract

Antifungal activity of the ethanol extract of bay-leaf (Syzygium  polyanthum (Wight) Walp.) against growth of the fungus Candida albicans in vitro has been conducted. Leaves of S. polyanthum has been known to have potential as antifungal for skin fungal pathogen. This study aims to determine antifungal activity of the ethanol extract of S. polyanthum leaves against growth of C. albicans.  Activity test was done by disc-diffusion  method and macro-dilution. This study used concentrations of ethanol leaf extract of S. polyanthum of 0.5%, 1%, 1.5%, 2%, and 2.5% (w/v). Negative control using 1% DMSO and positive controls using ketoconazole 30 mg/mL. The results shows that ethanol extract of leaves of S. polyanthum have activity as an antifungal. Ethanol extract of leaves of S. polyanthum based on the results of GCMS analysis of compounds containing chemical compounds such as terpenoids and fatty acids. Ethanol extract of leaves of S. polyanthum showed the highest inhibition zone diameter at a concentration of 1% (w/v) of 9.32 ± 0.21 mm. Value of Minimum inhibitory Concentration (MIC) for ethanol leaf extract of S. polyanthum present in a concentration of 0.5% (w/v) and the value of Minimum Fungicidal Concentration (MFC) present in a concentration of 1% (w/v).