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Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal
Published by Universitas Jenderal Soedirman
ISSN : 08531629     EISSN : 08531629     DOI : -
Core Subject : Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science
Majalah Ilmiah Biologi Biosfera : A Scientific Journal merupakan peer reviewed jurnal yang diterbitkan oleh Fakultas Biologi Universitas Jenderal Soedirman. Media ini mewadahi hasil-hasil penelitian di bidang biologi tropika yang terbit tiga kali setahun (Januari, Mei, September).
Arjuna Subject : -
Articles 342 Documents
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Keberhasilan Ginogenesis Ikan Tawes (Barbonymus gonionotus Blkr.) pada Dua Dosis Iradiasi UV (λ 254 nm) dengan Kejut Panas 40 C Reni Apriyani; Suhestri Suryaningsih; Yulia Sistina
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 33, No 3 (2016)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2016.33.3.346

Abstract

Laporan ini memverifikasi keberhasilan protokol tiga tahap ginogenesis tawes melalui inaktivasi genom jantan dengan iradiasi ultra violet (UV), fertilisasinya, dilanjutkan diploidisasi zigot dengan kejut panas (400 C) selama 60 detik. Eksperimen Rancangan Acak Lengkap, tujuh perlakuan yaitu, kontrol positif (fertilisasi normal); kontrol negatif1 telur difertilisasi dengan milt encer yang di UV 1983,348 J/m2; kontrol negatif2 dosis iradiasinya 3966,96 J/m2; ginogenesis1 kontrol negatif1 lalu dikejut panas pada 10 menit pasca fertilisasi; ginogenesis2 ginogenesis1 beda waktu kejut pada 15 menit pasca fertilisasi; ginogenesis3 kontrol negatif2 lalu dikejut pada 10 menit pasca fertilisasi; dan ginogenesis4 ginogenesis3 beda waktu kejut pada 15 menit pasca fertilisasi. Materi gamet segar diperoleh dari induk yang diinduksi GnRH analog dan domperindon dosis 1,5 ml/kg bobot induk intramuskuler, 6-10 jam sebelumnya. Milt segar diencerkan 100 x dalam larutan Ringer. Data dianalisis dengan uji Anova, menggunakan program SPSS. Hasil penelitian menunjukkan bahwa data fertilitas, data penetasan dan data kelangsungan hidup yang terbukti homogen (p>0,05), menunjukkan bahwa ketujuh perlakuan secara statistik fertilitas, penetasan, dan kelangsungan hidupnya tidak nyata (p>0,05) antar pelakuan. Data persentase morfologi larva abnormal tawes menunjukkan bahwa perlakuan yang dicobakan memberikan pengaruh yang sangat nyata (P<0,01). Secara keseluruhan dapat disimpulan bahwa, keempat perlakuan mitoginogenesis yang diujikan efektif menghasilkan larva mitoginogenesis ikan tawes walaupun efektifitasnya masih tergolong rendah. Efektivitas perlakuan dibuktikan dari signifikansi data morfologi larva abnormal dariklompok kontrol negatif membuktikan efektivitas dosis inaktivasi genetis jantan dan morfologi larva normal hasil perlakuan ginogenesis membuktikan efektivitas diploidisasi kejut panas tidak berbeda dari morfologi kontrol positif.
Identification of Sponge-Associated Bacteria with Antibacterial Property against Staphylococcus aureus based on Molecular Approach Ocky Karna Radjasa
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 24, No 3 (2007)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2007.24.3.282

Abstract

Staphylococcus aureus is among human pathogens which are known to be resistant to almost clinically significant antibiotics that causes a crisis, in the treatment and management of infectious disease, and also presents a clear danger to the future of public health. Recently, invertebrate-associated microorganisms have become the target of the search for marine microbial natural products. Sponge-associated microorganisms are one of the most interesting natural product sources because they produce bioactive natural products including polyketides and nonribosomal peptides. Molecular approach based on 16S rDNA has been very successful in the identification and the search of secondary metabolite-producing microorganisms, particularly by using specific degenerated primers, for the detection of Polyketide Synthases (PKS) and Nonribosomal Peptide Synthetases (NRPS) gene fragments that are essential for the biosynthesis of bioactive products. Four bacterial isolates from sponge Dysidea sp. were found to inhibit the growth of S. aureus. However, only the best two isolates were selected for further identification and screening for the presence of either PKS or NRPS. The results revealed that both isolates BSP5.11 and BSP11.7 have 99% homology with Alpha proteobacterium. PCR analysis of PKS and NRPS gene fragments showed that they both amplified the NRPS but not the PKS gene fragments.
Screening of Potential Probiotic Vibrio sp. Against Vibriosis in the Litopenaeus vannamei Munti Sarida; Esti Harpeni
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 27, No 2 (2010)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2010.27.2.197

Abstract

Vibriosis is a bacterial disease generated by Vibrio sp., particularly V. harveyi that becomes the biggest cause of up to 100% shrimp mortality. Using antibiotics as the curative step is not applicable anymore, because of the residual effects to the shrimp and environment and the resistant effects to the humans as well. The purpose of this study was to screen potentially probiotic from strain Vibrio sp. that could inhibit V. harveyi as an effort of handling vibriosis diseases. Water sample from the shrimp ponds were isolated and purified in SWC-agar and TCBS-agar and then were incubated overnight at the room temperature. Bacterial concentrations were calculated using MC Pharland method and spectrophotometer. In vivo test was conducted for the best candidate of potential probiotic isolate, i.e. CP1. Challenged test was leaded within 4 different bacterial concentrations as the treatments and 2 replications using 10 shrimps as the tested organisms per aquarium. The treatments were 107 CFU/ml CP1 VS 107 V. harveyi MR5339 RifR (1), 106 CFU/ml CP1 VS 107 V. harveyi MR5339 RifR (2), 105 CFU/ml CP1 VS 107 V. harveyi MR5339 RifR (3) and PBS (4) as the control.  Our study showed that the Survival Rate of the tested organisms was 90% in the treatment 1. This result indicated the capability of growth inhibition of the candidate probiotic bacterium against V. harveyi. Presumably, Vibrio sp. could be used in the vanamei culture as the probiotic. Finally, the candidate probiotic bacterium was identified as Vibrio furnissii.
Pengaruh Limbah Organik terhadap Status Tropik, Rasio N/P serta Kelimpahan Fitoplankton di Waduk Panglima Besar Soedirman Kabupaten Banjarnegara Endang Widyastuti; Sukanto Sukanto; Nuning Setyaningrum
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 32, No 1 (2015)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2015.32.1.293

Abstract

The increased of organic substance pollutans in waters as one of stimulate factors for phytoplankton growth. The input of organic matter from antrophogenic  activity tend to influence the trophic state of reservoir. The aims of this research are to examine the influence of organic waste to Panglima Soedirman reservoir concerning the trophic state, Redfield ratio N and P, also the relation with abundance of phytoplankton. This research used survey methods with purposive sampling technique on 7 obsevation stations with 3 repeating conducted on May-July 2013. The result of the research showed that the input of organic matter increased in BOD5, COD and ammonia in reservoir and trophic state. Redfield ratio was shown 19,13-65,82 which means P as a limiting factor. Relationship between N/P with the abundance of phytoplankton with degree was strong correlated with chlorophyta and chrysophyta.
Pengaruh Lama Inkubasi Soyghurt Menggunakan Inokulan dengan Penambahan Bifidobacterium sp. terhadap Daya Hambat Bacillus cereus Ika Fitriani; Dyah Kusharyati; Pancrasia Maria Hendrati
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 33, No 1 (2016)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2016.33.1.287

Abstract

Bakteri asam laktat (BAL) yang digunakan dalam pembuatan soyghurt di antaranya     Lactobacillus bulgaricus, Streptococcus thermophillus, dan Bifidobacterium sp. dengan suhu optimal pertumbuhan 36°-37°C dan lama inkubasi berkisar 1-2 hari. BAL diketahui mampu menghasilkan bakteriosin yang dapat menghambat bakteri patogen dalam saluran pencernaan salah satunya ialah Bacillus cereus. Tujuan penelitian ini ialah mengetahui perbandingan BAL pada soyghurt terhadap daya hambat B. cereus, mengetahui lama inkubasi soyghurt dengan penambahan Bifidobacterium sp terhadap pertumbuhan B. cereus, dan mengetahui pengaruh konsentrasi BAL dan lama inkubasi pada soyghurt terhadap pertumbuhan B. cereus. Hasil penelitian menunjukkan perbandingan BAL (L. bulgaricus : S. thermophillus : Bifidobacterium sp.) 1:1:1 (K1) pada soyghurt mampu menghambat B. cereus. Lama inkubasi soyghurt dengan penambahan Bifidobacterium sp. optimal pada 24 jam terhadap pertumbuhan B. cereus. Konsentrasi BAL dengan lama inkubasi pada soyghurt tidak mampu meningkatkan daya hambat pertumbuhan B.cereus.
Kualitas Air Pasca Pengerukan Alur Transportasi Batu Kapur di Sungai Donan Cilacap Mohammad Husein Sastranegara; Sri Lestari
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 26, No 1 (2009)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2009.26.1.90

Abstract

Pengaruh Kombinasi Jenis Bahan Pembawa dan Lama Masa Simpan yang Berbeda terhadap Produksi Pelet Biofungisida Trichoderma harzianum Juni Safitri Muljowati; Purnomowati Purnomowati
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 27, No 1 (2010)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2010.27.1.188

Abstract

This research aimed to know effect of carrier material combination and different storage duration on viability of T. harzianum, also to know the material type and storage duration which yielding highest conidia.  Method used in this research was experimental by using Completely Randomized Design (CRD) with factorial pattern. The first factor was type of carrying material that were white sticky rice meal, mix of 75% white sticky rice meal and 25% mungbean meal, mix of 75% white sticky rice meal and 25% soybean meal, and mix of 75% white sticky rice meal and 25% skim milk that each of such material was inoculated by 108 conidia/ml of T. harzianum with storage duration levels that were 0 weeks, 3 weeks, 6 weeks, and 9 weeks.  Obtained data was analyzed by analysis of variance (F test) with significantly level 95% and 99% and followed by Honestly Significant Difference (HSD).  Result showed that there was interaction between types of carrying material with pellet storage period that showed significant effect on viability of T. harzianum.  Carrying material type of white sticky rice meal with pellet storage period of 9 weeks resulting highest percentage acerrage of conidia seedling (viability) of T. harzianum that was 78,19%.
Keanekaragaman Bakteri Tanah dari Teluk kodek Area, Pamenan Lombok Barat Tri Ratna Sulistiyani
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 28, No 3 (2011)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2011.28.3.280

Abstract

Indonesia is the center of the world's biodiversity with a unique biodiversity and priceless. To explore and get more information about biodiversity, strategic research was needed.  The objective of this reseach was to explore the population diversity of soil microbes in Lombok Island.  Bacterium isolates was identified by molecular 16S rDNA. Soil samples from 5 different sites in Lombok Island showed various bacteria population. The highest population 113 x 106 CFU/g soil was found in soil sample around Plumeria acuminata and the lowest 34 x 106CFU/g soil was found in soil sample around  Tamarindus indica tree.  Fourteen of isolates were identified using molecular identification with homology from 94 – 100%.
Pemantauan Keanekaragaman Hama dan Musuh Alami pada Ekosistem Tepi dan Tengah Tanaman Kacang Tanah (Arachis hypogaea L.) Eko Apriliyanto; Sarno Sarno
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 35, No 2 (2018)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (430.485 KB) | DOI: 10.20884/1.mib.2018.35.2.603

Abstract

Keberadaan hama dan musuh alami pada tanaman budidaya dapat sebagai acuan dalam kegiatan pengendalian hama tanaman. Penelitian ini bertujuan untuk mengetahui keanekaragaman hama dan musuh alami ekosistem tepi dan tengah permukaan tanah pada pertanaman kacang tanah (Arachis hypogaea L.) yang dipantau dengan memasang perangkap pitfall trap. Penelitian dilakukan di lahan Politeknik Banjarnegara yang berlokasi di kelurahan Kenteng, kecamatan Madukara, kabupaten Banjarnegara, 323 m dpl. Penelitian dengan dua perlakuan berupa lokasi penempatan pitfall trap pada bagian tepi dan tengah lahan tanaman kacang tanah. Lahan tanaman kacang tanah dengan ukuran 40 m x 50 m dan tanaman berumur 28 hari setelah tanam. Analisis data dilakukan dengan menghitung indeks keanekaragaman Shannon-Weaver (H’). T-test digunakan untuk mengetahui perbedaan yang signifikan antara populasi hama dan musuh alami yang berada pada tepi dan tengah lahan kacang tanah. Populasi hama dan musuh alami permukaan tanah pada tanaman kacang tanah mempunyai  tingkat keanekaragaman yang rendah dengan nilai H’<1,0. Nilai indeks keanekaragaman tersebut rendah baik pada populasi hama dan musuh alami tepi lahan maupun tengah lahan kacang tanah. Indeks keanekaragaman hama, musuh alami dan kelompok lainnya pada tepi lahan dengan nilai 0 hingga 0,32; sedangkan pada tengah lahan dengan nilai 0 hingga 0,30.
Uji Antimikroba Rumput Mutiara (Hedyotis corymbosa) terhadap Bakteri dan Jamur Penyebab Penyakit pada Ternak Unggas Nurhayati Nurhayati; Madyawati Latief; Heru Handoko
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 23, No 3 (2006)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2006.23.3.172

Abstract

An experiment was conducted to determine the activity of pearl grass (rumput mutiara, Hedyotis corymbosa) in inhibiting growth of bacterial and fungi causing diseases in poultry.The current study used 3000 gram of sorted and cleaned pearl grass from dust and other weeds. This study employed sample collection, extraction, fractionation, preparation of medium of Agar and Mueller Hinton Broth, culturing bacterial, antimicrobial test and measuring minimum inhibitory concentrations. Extraction was conducted by maceration of pearl grass with methanol for five days followed by evaporating in vacuo to get methanol extract. The extract was then fractionated by adding hexane or acetic acid and ethyl acetic or sodium bicarbonate or n-buthanol to get fraction of hexsane, acid ethyl acetic, base ethyl acetic, n-buthanol. Each fraction was then tested with microbial test that were Salmonella sp, Escherichia coli, and Candida albicans. The results showed that each fraction at 2 % of concentration could inhibit bacterial growth except for Candida albicans that was not inhibited by any fraction of pearl grass. The minimum inhibitory concentrations was 0.4 – 0.8% (4 mg/ml – 8 mg/ml) indicating that pearl grass fractions were highly active on inhibiting bacterial growth that cause poultry diseases and the inhibiting activity on E. coli was found higher than that on Salmonella sp.

Page 20 of 35 | Total Record : 342

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