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Jurnal Perikanan Universitas Gadjah Mada
ISSN : 08536384     EISSN : 25025066     DOI : -
Jurnal Perikanan Universitas Gadjah Mada are published to promote a critical review of the various investigative issues of interest in the field of fisheries between the researchers, academics, students and the general public, as a medium for communication, dissemination, and utilization of wider scientific activities.
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Articles 12 Documents
Search results for , issue "Vol 11, No 2 (2009)" : 12 Documents clear
Pemijahan dan Perkembangan Embrio Ikan Pelangi, Melanotaenia spp. Asal Papua Chumaidi, Chumaidi; Nur, Bastiar; Sudarto, Sudarto; Pouyaud, Laurent; Slembrouck, Jacques
Jurnal Perikanan Universitas Gadjah Mada Vol 11, No 2 (2009)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (34.127 KB) | DOI: 10.22146/jfs.3

Abstract

Rainbow fish (Melanoptaenia spp.) originated from Sungai Gelap of Papua. The objective of this experiment was to know the fecundity, hatching rate and the rate of embryogenesis. Total of 50 male and female broodstock measuring 10–15 cm reared in closed recirculating water system in concrete tank until their gonad matured. One pair composed of one male and two female’s of matured fish was transferred to 1 x 1 x 0.5 m³ concrete tank for pair breeding, the fish was sheltered with water hyacinth. Released eggs were observed in the morning and in the afternoon daily. Eggs were categorized into fertile and infertile. Some 25 eggs were incubated in plastic baskets, and observed under microscope for their embryonic development stages. The results showed that rainbow fish from Sungai Gelap produced eggs (averages) 392 per female; fertility rate was 93.74%; average hatching rate was 87.36%, and the fertilized eggs were hatched approximately 5 days incubation.
Pembenihan Selektif Kerapu Bebek, Cromileptes altivelis Turunan ke-2 (F-2) Tridjoko, Tridjoko; Haryanti, Haryanti; Muzaki, Ahmad; Sutarmat, Tatam
Jurnal Perikanan Universitas Gadjah Mada Vol 11, No 2 (2009)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (34.127 KB) | DOI: 10.22146/jfs.4

Abstract

The aim of this experiment was to know the gonad development of humpback grouper Cromileptes altivelis F-2 progeny. The F-2 progeny fishes were produced from selective breeding and composed by Lot 1, Lot 2 and Lot 3. The fishes were reared in the cylinder concrete tanks containing 75 m³, 10 m³ water and floating cages. Fishes were fed with commercial dry pellet in addition with vitamin mix, vitamin C and vitamin E. The result indicated that the mature gonad female of F-2 grouper broodstocks have been achieved. The female broodstocks with 590 g weight produced oocytes with diameter more than 400 µm. Genetic analysis showed that grouper Lot 2 was homogenous, did not find any polymorphism among individual fish, however Lot 1 and Lot 3 still showed high heterozygocity as 0.18 and 0.58, respectively.
Karakterisasi Tiga Lokus Mikrosatelit pada Telur dan Larva Tuna Sirip Kuning, Thunnus albacares Moria, Sari B.; Permana, Gusti N.; Hutapea, Jhon H.
Jurnal Perikanan Universitas Gadjah Mada Vol 11, No 2 (2009)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (34.127 KB) | DOI: 10.22146/jfs.5

Abstract

Characterization of three microsatellite loci are able to provide information about genetic variation or polymorphism of yellow fin tuna eggs and larvae produced by domestication of broodstock. Thirty samples of eggs and larvae were collected from different spawning day and analyzed. Amplification of those three loci were carried out with multiflexing technique and electrophoreted ABI Prism 310 Genetic Analyzer sequencer. From those three loci were used (Ttho-1, Ttho-4 and Ttho-7) showed that locus Ttho-7 had higher genetic variance (0.720-1.212) compared to others loci. Based on the result, it can be concluded that high allele frequency as showed at Ttho-7 could be used as marker for  parental analysis.
Kondisi Terumbu Karang dan Biota Lainnya di Perairan Kecamatan Selat Nasik Kabupaten Belitung Tahun 2007-2008 Sjafrie, Nurul D. M.
Jurnal Perikanan Universitas Gadjah Mada Vol 11, No 2 (2009)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (34.127 KB) | DOI: 10.22146/jfs.6

Abstract

The aims of  this study was to monitor the coral reef condition, coral fishes and benthic organisms in Selat Nasik Sub District waters. The sampling  was carried out on March 2008 in 12 stations at Selat Nasik waters that were similar to sampling station for 2007 study. Observations on benthic lifeform was done using  LIT (line intercept transect), coral fishes was observed using Underwater Visual Census (UVC) whereas benthic organisms was counted  using Belt Transect. The result  showed that  the coral reef condition in Selat Nasik Sub District water’s was still in good condition. Coral Fishes increased in number, especially target fishes and indicator fishes. Bentic organisms such as Diadema and Fungia increased in number, whereas Tridacna decreased as well.
Penggunaan Gen GH sebagai Marka Molekuler DNA Gurami, Osphronemus goramy dalam Pengembangan Teknologi Surrogate Broodstock Achmad, Marlina; Alimuddin, Alimuddin; Carman, Odang; Arfah, Harton; Zairin, Muhammad
Jurnal Perikanan Universitas Gadjah Mada Vol 11, No 2 (2009)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (34.127 KB) | DOI: 10.22146/jfs.7

Abstract

The technology of fish germ cell transplantation had been established to create broodstock systems by which a target offspring can be produced from a surrogate parent. Donor cell for transplantation is derived from transgenic fish carrying green fluorescent protein gene functions as a marker to distinguish the donor from recipient cell. In this study, an alternative technique was developed for identifying gouramy-derived donor cell and Nile tilapia as recipient by PCR amplification method using growth hormone (GH) gene as a molecular marker.  Specific primer for GH gouramy was designed by using Genetyx version 7 software.  ß-actin gene was used as an internal control of DNA loading. The result showed that a specific PCR amplification product of 340 in length was obtained when the DNA template was from gouramy, while no PCR product from Nile tilapia. The minimum concentration of genomic DNA of gouramy mixed with a 700 ng/µl of Nile tilapia that could be detected by PCR was 1 ng/µl. Thus, PCR method with specific GH primer may be useful to detect the incorporation of donor cell in recipient gonad towards development of surrogate broodstock technology.
Biodiversity Different and Species Assemblages of Phytoplankton Within Ooze Sediment at Myll Lakes, New South Wales Australia Rukminasari, Nita
Jurnal Perikanan Universitas Gadjah Mada Vol 11, No 2 (2009)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (34.127 KB) | DOI: 10.22146/jfs.8

Abstract

Phytoplanktons are primary producer, which determine the waters productivity. The assemblages of phytoplankton in the lake varied spatially and temporally as a result of nutrient concentration, some physical and chemical factors. However, the assemblage of phytoplankton in the lake was not well documented.  The overall aim of the study was  to count and identify phytoplankton taxa and to examine assemblages at location with and without ooze, and to relate the presence/absence of ooze to phytoplankton abundance and assemblages. The samples were collected from Myall Lakes, NSW Australia. The data were analysed using multivariate analysis to determine assemblages of phytoplankton amongst location. Furthermore, univariate analysis was used to examine how the water quality was in particular presence/absence of ooze affected to phytoplankton abundance. The hypothesis being tested was: (1) phytoplankton assemblages vary spatially in Myall Lake due to varying water quality characteristics, including presence of ooze, (2) there was a significant difference of phytoplankton abundances between locations due to presence/absence of ooze.In both instance the hypothesis on this study was accepted. There was a significant different not only phytoplankton assemblages but also phytoplankton abundance within locations. It concluded that the phytoplankton varied spatially in those locations. Presence/absence of ooze affects abundance of phytoplankton. However, further study was needed to conduct in particular to determine certain aspects which affect the assemblages and abundances of phytoplankton.
Population of Esteochilus spp. as a Base of Sustainable Fishing in Serayu River Lestari, Windiarini; Putranto, Yogi; Setyaningrum, Nuning; Sugiharto, Sugiharto
Jurnal Perikanan Universitas Gadjah Mada Vol 11, No 2 (2009)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (34.127 KB) | DOI: 10.22146/jfs.9

Abstract

The increasing demand for freshwater resources generated by human population growth, urbanization, industrialization and irrigation will result in a decline or loss of freshwater fish such as Osteochilus spp. The aims of research were to analysis the population of Osteochilus spp in terms of abundance, longitudinal distribution and sex ratio and to determine environmental factors of river driven these populations. The research was carried out in upper part of Serayu River during wet season (January – March) and dry season (June - August) 2007. Fish was collected by using electro fishing and stratified random sampling was applied by dividing this river into five strata based on altitude. The result showed that the total abundances of Osteochilus spp. was 149 individuals, it was composed by of O. hasselti (47) with 8.30 – 24.35 cm and 11.20 – 198.70 g, O. microchephalus (101) with 11.35 – 25.21 cm and 17.9 – 206.5 g and O. kahajanensis (1) with 17.30 cm and 68.80 g. The size of population O hasselti and O. microchephalus gradually increased from 7 and 4 individuals in stratum A became 27 and 12 individuals in stratum D, and then increased up to 43 and 17 individuals in stratum E. The sex ratio of O. hasselti was 10:16 and O. microchephalus was 20:23. Both species were longitudinally distributed in Serayu River. It seems that the population of O. hasellti and O. microchephalus were more influenced by physical factors i.e. depth and current rather than chemical factors i.e. DO and pH of Serayu River.
Pengaruh Salinitas terhadap Perkembangan Parasit pada Benih Gurami, Osphronemus goramy Rahayu, Nani S.; Susanti, Dewi; Lantiani, Dwi; Wibowo, Sutopo A.; Diana, Roosita; Murwantoko, Murwantoko
Jurnal Perikanan Universitas Gadjah Mada Vol 11, No 2 (2009)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (34.127 KB) | DOI: 10.22146/jfs.10

Abstract

Research was conducted to know the effect of salinity on the reduction of parasites in Gouramy (Osphronemus goramy). The Research was performed using completely Randomized design with 5 salinity treatments (water pond, water with salt concentration 0, 2, 4 dan 6 g/L) with 4 replications. The 5-7 cm of gouramy fingerling were reared as on treatments and the parasites were counted  using skin scrapping dan wet mount methods. Observation was performed every 3 day for 15 days on parasite intensity, prevalence, mortality, symptoms and water quality.The result showed that several parasites including Trichodina sp., Apiosoma sp., Ichthyophthirius sp., Oodinium sp., dan Henneguya sp were observed. The highest intensity and prevalence was found in Trichodina sp. The Salinity and lenght administration were significantly decrease number parasites.  Salinity treatments (2, 4 dan 6 g/L ) for 3 days could totally removing Trichodina sp. from fishes. Meanwhile the data of other parasite species were unsignificant due to the low level of its intensity and prevalence. Salinity administration in level 6 g/L could reduce mortality.
Identifikasi Cacing Polychaeta, Nereis sp. sebagai Vektor White Spot Syndrome Virus (WSSV) di Alam dan Kajian Uji Tantangnya di Laboratorium Bambang W. Prastowo; Kade Ariawan; Evy M. Nur; Rahayu Rahardianti; Yuni Setyowati
Jurnal Perikanan Universitas Gadjah Mada Vol 11, No 2 (2009)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (367.973 KB) | DOI: 10.22146/jfs.11

Abstract

Polychaete worm is an important zoobenthos at shrimp culture system, however since there is no any culture of these worm in Indonesia, then all the worm biomass collected from their natural habitat. It raised some concern about their safety because polychaete worm might be get infected and will realistic as a pathway for virus to attack shrimp broodstock in the hatchery. Fresh polychaete worms (1 g wet) used as a test organisms were caught from pond area in Semat region, Jepara. The step of these experiment are identification of WSSV infection at polychaete worms in wild with PCR analysis, visual, microscope and histology observation. Followed by challenge test of polychaete worm with WSSV, and WSSV infectivity study at black tiger shrimp broodstock. It seen, from the experiment, that using two different PCR methods, OIE (2006) and Nugen kit, and also histology analysis showing no WSSV infection in polychaete worms. However, from further experiment showing that polychaete worm challenge by WSSV have already got severely stress due to WSSV inocculum treatment, but the level of infection is still very low so that can not detect by PCR. Shrimp broodstock that have already fed with WSSV-contaminated polychaete worms for 1 week, based on clinical signs seems to get infected by WSSV, even the infectivity level is still very low. It concluded that polychaete worm can be a vector for WSSV in pond. 
Efektivitas Kanamycin terhadap Furunculosis pada Karper, Cyprinus carpio Sri Retnoningsih; Kamiso H. Nitimulyo; Kardiman Lanadimulya; Suprayogi Suprayogi; Supardi Supardi; Djoko Darmantani; I P. Panca; Hasnah Hasnah; Soefaad Soefaad; Milis Milis
Jurnal Perikanan Universitas Gadjah Mada Vol 11, No 2 (2009)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1940.89 KB) | DOI: 10.22146/jfs.12

Abstract

The aim of experiment was to know the appropriate and effective dosis of kanamycin antibiotic in order to recover and clean the Aeromonas salmonicida which caused furunculosis in carp, Cyprinus carpio. The experiment consisted of the preliminary test [revirulence of A. salmonicida, sensitivity, minimum inhibitory concentration (MIC), minimum bactericidal concentration test (MBC), lethal concentration-50 (LC50) of A. salmonicida to carp] and the effectiveness antibiotic kanamycin test. The water quality was analyzed at the beginning and the end of the experiment, histopathology was analyzed at the end of the experiment. The data were analyzed using analysis of variance (ANOVA) continued by least significant different and regression tests.Based on the result of in vitro test, it was obtained that the value of MIC was 4 ppm and the value of MBC was 8 ppm, while the result of lethal concentration-50 (LC50) A. salmonicida to carp was 1.07 x 105  cell/ml. Survival rate of carp at the end of experiment were 20% (treatment 0 ppm); 73,3% (treatment 16 ppm); 86.7% (treatment 32 ppm); 93.3% (treatment 48 ppm); 96.7% (treatment 64 ppm); and 100% (treatment 80 ppm). The survival rates were very significance difference (P <0,01) among treatments and the treatments of kanamycin were very significant difference (P <0.01) from positif control. The regression showed that there was correlation between the concentration of kanamycin and survival rates of carp by coefficient of correlation (R2) at 0.9382.

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