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INDONESIA
Jurnal Perikanan Universitas Gadjah Mada
Published by Universitas Gadjah Mada
ISSN : 08536384     EISSN : 25025066     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
Jurnal Perikanan Universitas Gadjah Mada are published to promote a critical review of the various investigative issues of interest in the field of fisheries between the researchers, academics, students and the general public, as a medium for communication, dissemination, and utilization of wider scientific activities.
Arjuna Subject : -
Articles 460 Documents
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ISOLASI, KARAKTERISASI, DAN PATOGENITAS BAKTERI PENYEBAB PENYAKIT PADA GURAMI (Osphronemus goramy) DI KABUPATEN BANTUL Murwantoko Murwantoko; Rozi Rozi; Indah Istiqomah; Kamiso H. Nitimulyo
Jurnal Perikanan Universitas Gadjah Mada Vol 15, No 2 (2013)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.9102

Abstract

Gouramy (Osphronemusgoramy) is an important fish comodity and preferred to be consumed by people. One of the limiting factor in freshwater aquaculture is bacterial infections. The objective of this research was to identify the species of pathogenic bacteria on gourami from Bantul Regency and to determine their pathogenicity. Eighteen diseased gouramy were collected from seven different fi sh farms. Bacteria were isolated from kidney and purifyon TSA medium. Characterization was conducted on the morphology of bacterial colonies and cells, biochemical tests. Koch Postulate Test was done to confirm pathogenic properties of the isolated bacteria. Pathogenicity test was performed by intraperitoneal injection of a serial doses of bacteria into gouramy and the Lethal Dosage 50 (LD50) was calculated. Twenty isolates of Aeromonas hydrophila, 5 isolates of Aeromonas salmonicida and one isolate of Vibrio cholerae,Vibrio anguillarum and Pseudomonas fluorescens respectively were isolated from and confi rmed as pathogen ingouramy. Pathogenic diversity was found among isolated bacteria, which a group of virulent bacteria as Aeromonas hydrophila GBNB, GGRB, and GCMA3 with LD50 values of 6.28 x 105, 2.09 x 105 and 5.37 x 105 CFU /fish; and a group of non virulent bacteria as A. hydrophila GKRA isolate and Vibrio cholera GPDA isolat with LD50 value of 7.14 x 107 and 3.45 x 107 CFU/fish respectively.
JENIS DAN SEKS-RASIO UDANG BARONG (Panulirus spp.) DI KAWASAN PANTAI GUNUNG KIDUL, YOGYAKARTA Sukiman Wirosaputro
Jurnal Perikanan Universitas Gadjah Mada Vol 1, No 1 (1996)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.8841

Abstract

Activity researched for lobster in coastal area Gunungkidul Yogyakarta began November, 1995 to Februari, 1996. Number of lobsters to identify were 3,755 head for species and 1,525 head for sex-ratio. Number of species were 6kinds, there were : Panulirus pencillatus (56.48%), P. homarus (30.38%), P. longipes (6.73%), P. ornatus (5.40%), P, versicolor (0.95%), P. polyphagus (0.06%). General sex-ratio between male:female = 1.83:1.00. number of female carrying eggs 3.40% and not carrying eggs 32.54%. Sex-ratio for each species between male:female for P. penicillatus, P. homarus, P. longipes, P. ornatus, P. versicolor, P. polyphagus, were = 1.83:1.00 ; 1.71:1.00 ; 2.20:1.00 ; 0.97:1.00 ; 2.40:1.00 ; 1.00:0.00 respectively.
Pengaruh Suhu terhadap Kelangsungan Hidup dan Percepatan Metamorfosis Larva Kepiting Bakau (Scylla olivacea) Muhammad Y. Karim; Zainuddin Zainuddin; Siti Aslamyah
Jurnal Perikanan Universitas Gadjah Mada Vol 17, No 2 (2015)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.10370

Abstract

Kepiting bakau merupakan salah satu komoditas perikanan bernilai ekonomis penting di kawasan AsiaPasifik. Penelitian ini bertujuan untuk menentukan suhu optimal bagi pemeliharaan larva kepiting bakau (Scylla olivacea). Penelitian dilaksanakan di Balai Perikanan Budidaya Air Payau (BPBAP), Kecamatan Galesong, Kabupaten Takalar, Sulawesi Selatan. Hewan uji yang digunakan adalah larva kepiting bakau (S. olivacea) stadia zoea-1 yang ditebar dengan kepadatan 50 ekor/l dan dipelihara sampai memasuki stadia megalopa. Wadah penelitian menggunakan baskom plastik hitam berkapasitas 50 l diisi air media bersalinitas 30 ppt sebanyak 35 l yang dilengkapi dengan thermostat untuk mempertahankan suhu media percobaan. Pakan yang digunakan adalah pakan alami berupa rotifer dan nauplius Artemia dan pakan buatan berupa flake. Penelitian dirancang dengan menggunakan rancangan acak lengkap dengan 4 perlakuan dan masing-masing perlakuan mempunyai 3 ulangan. Keempat perlakuan yang dicobakan adalah perbedaan suhu media pemeliharaan larva kepiting bakau, yaitu : 26, 28, 30, dan 32oC. Hasil analisis ragam menunjukkan bahwa suhu media pemeliharaan berpengaruh sangat nyata (p < 0,01) pada tingkat kelangsungan hidup dan kecepatan metamorfosis larva kepiting bakau (S. olivacea). Tingkat kelangsungan hidup tertinggi dan perubahan metamorfosis larva kepiting bakau dari zoea ke megalopa tercepat dihasilkan pada suhu 30 oC yakni 31,60% dan 17 hari, sedangkan tingkat kelangsungan hidup terendah dan perubahan metamorfosis terlama pada suhu 26 oC yakni 18,89% dan 20,67 hari.
KELIMPAHAN FITOPLANKTON DI PERAIRAN MAITARA, KOTA TIDORE KEPULAUAN Yuliana Yuliana
Jurnal Perikanan Universitas Gadjah Mada Vol 10, No 2 (2008)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.8898

Abstract

The aim of this research were to know the correlation between water physic-chemistry and the abundance of phytoplankton. The studies were conducted from May to September 2006 at Maitara water and samples were taken from 5 (five) stations for 4 (four) period by filtration method. The result showed that there were 12 genera of phytoplankton from 4 classes: Bacillariophyceae (7 genera), Chlorophyceae (1 genera), Cyanophyceae (1 genera), and Dinophyceae (3 genera). The phytoplankton abundance ranged from 5781 – 39227 individual/L, the highest value was on station 5 period III (39227 individual/L) and the lowest on station 3 period IV (5781 individual/L). The range values of phytoplankton biological index such as diversity index (H’) was 0.6889 – 1.1841, equitability index (E) = 0.4969 – 0.7215, and dominant index (D) = 0.3856 – 0.6183. Physical-chemistry parameters of the water were 0.01 – 2.24 mg/L of nitrate, 0.09 – 0.28 mg/L of orthophosphate, 0.040 – 0.042 of silicate, 6.21 – 8.27 of pH, 30 – 35 ‰ of salinity, 28.4 – 31.5oC of temperature, and 1.10 – 2.75 m of transparency, respectively. Multiple regression analysis showed that there was relationship between waters physical-chemistry parameters and phytoplankton abundance (R2 = 0.796), where the regression equation was Y = 150190.2 + 36.589 nitrate + 597.043 orthophosphate + 1002598 silicate + 25.289 pH - 23.866 salinity – 561.222 temperature – 56.973 transparency.
PRODUKSI DAN APLIKASI VAKSIN DNA Streptococcus iniae UNTUK MENINGKATKAN IMUNITAS NILA (Oreochromis niloticus) Sutanti Sutanti; Novi Megawati; Sugiyo H. Pranoto; Ratu Siti Aliah
Jurnal Perikanan Universitas Gadjah Mada Vol 18, No 2 (2016)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.13049

Abstract

DNA vaccine Streptococcus iniae is a third generation of vaccines based on the gene encoding a vaccine antigen. Pgm is DNA-binding protein that activates expression of several important virulence gene, including those encoding polysaccharide capsule. The pgm gene controlled by MBA promoter has constructed successfully as a candidate of DNA vaccine to against S. iniae infection in Nile Tilapia. The goals of this study were to production and application of DNA Vaccine S. iniae to increase the nile tilapia immunity.  Vaccine production was using plasmid isolation method from Escherichia coli dH5α containing pMBA-pgm. Vaccine verification was was accomplished by PCR using pgm gene S. iniae Fstrep and Rstrep specific primers, which revealed the 1,713 bp PCR product. Application of DNA vaccine was using 25, 50, 75, and 100ng/ml dosage with intraperitonial injection method .The challenge test was using 108 cfu/ml density of S. iniae. Observation test parameter were survival rate, relative percent survival, mean time to death, pgm gene, and organ histology such as liver, eyes, brain, spleen and kidney. Vaccine production was succeed using plasmid isolation method and containing pgm gene revealed 1713bp. the application of DNA vaccine was optimum at 50ng/ml dosage with SR value 96.667%, RPS value 88,461% and MTD value 4,6 days. The eyes histology shows opacity and exopthalmia, the other organ such as liver, spleen and kidney was necrosis. The DNA vaccine was optimum at 50ng/ml dosage.  
Protein Aeromonas Hydrophila Sebagai Vaksin untuk Pengendalian Mas (Motile Aeromonas Septicemia) pada Jambal Siam (Pangasius hypophthalamus) Olga Olga; Ririen Kartika Rini; Junius Akbar; Alim Isnansetyo; Langkah Sembiring
Jurnal Perikanan Universitas Gadjah Mada Vol 9, No 1 (2007)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.59

Abstract

Objectives of this study were to find out the protein of Aeromonas hydrophila for vaccine, and to evaluate the efficacy of the protein to control Motile Aeromonas Septicemia (MAS) in catfish (Pangasius hypophthalamus). A. hydrophila was isolated from the kidney of diseased catfish. The isolate was cultured, sonicated and sentrifuged into cell-free extract and debris. The protein in the cell-free extract was precipitated with ammonium sulphate, dialized, then fractionated by gel filtration column chromatography on Sephadex G150.The concentration of protein were estimated by spectrophotometer at 280 nm. Fractions obtained from the gel filtration with high concentration of protein were analized by sodium dodocyl sulphate-polyachrylamide gel electrophoresis (SDS-PAGE). The protein bands obtained from SDS-PAGE with molecular weight more than 10 kDa were tested for immunogenicity to mice. Futhermore, the most immunogenic protein was used for fish vaccination. The vaccinated and unvaccinated fishes were chalenged with A. hydrophila.The results indicated that vaccination with the protein significantly increased (P<0.01) the antibody titer either in mice or catfish. Relative Percent Survival of catfish vaccinated with the protein at 5; 7.5; and 10 µg/fish were 61.54%, 80.77% and 76.92%, respectively. The optimum dose of vaccine was 8.33 µg/fish with maximum RPS of 82.05%.
PEMANTAUAN PLANKTON DAN KUALITAS AIR TAMBAK UDANG WINDU INTENSIVE DI LAHAN PASIR PANTAI Bambang Triyatmo
Jurnal Perikanan Universitas Gadjah Mada Vol 5, No 1 (2003)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.8983

Abstract

A research was conducted in intensive ponds of shrimp (Penaeousmonodon) culture located in sandy coastal area Pandansimo, Bantul Regency. Shrimp was cultured in two ponds (± 3.600 m2 each). The bottom of the first pond was 5-10 cm of soil. The second pond used sand originated from the coastal area as the bottom. Shrimps were cultured in the two ponds for 4.5 months. During the culture, plankton and water quality of ponds was observed for 3 times, namely on early, middle and final of shrimp culture.Result of the experiment indicated that 1). Density of plankton in both increased during shrimps culture; 2). Density of plankton in the pond with soil bottom was higher than in that of the pond with sand bottom; 3). The productivity of ponds increased; 4). As higher plankton density increase the absorption of NH4 and PO4, the availability of these nutrient gradually lowered; 5). Species composition of plankton in ponds with soil bottom was dominated by Spirulina sp. Brachionus sp., and Chlorella sp.; 6) In addition species composition of plankton in ponds with sand bottom was dominated by., Brachionus sp., Chlorella sp., Spirulinasp., and Cyclotella sp.; 7) During shrimp culture, the water qualities of the ponds were commonly in the optimal condotions for shrimp; 8) Water quality for the first three months of culture generally was suitable for shrimp culture both in pond with soil and sand bottom. However, after 90 days of culture, the dissolved oxygen in ponds with soil and sand bottom ranged from 2.0 to 3.0 mg/l, which was unsuitable for shrimp culture; 9) The production, survival rates and average body weights of shrimp were 3.18 ton/ha, 12.23 g and 42.3% from pond with soil bottom, and 3.51 ton/ha, 14.43g and 43.38% from pond with sand bottom, respectively.
Jumlah dan Kualitas Kista Artemia pada Berbagai Tingkat Perubahan Salinitas Dade Jubaedah; D. Djokosetiyanto; A. Fairus Mai Soni
Jurnal Perikanan Universitas Gadjah Mada Vol 8, No 2 (2006)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.140

Abstract

The objective of this research was to know the optimum salinity changes to improve quantity and quality of cyst produced by Artemia. This experiment used completely randomized factorial design. Factor a was salinities changes at day-9 (A) and day-15 (B) of culture. Factor b consisted of four salinity increasing treatments, which were I (100, 100, 100, 140 ppt); II (100, 100, 140, 140 ppt); III (100, 140, 140, 140 ppt) and IV (100, 110, 125, 140 ppt). The highest fecundity was obtained by B.I treatment. The highest quantity of the cysts was produced by A.III treatment. Treatment of A.I produced cysts with the highest protein content. These results showed that the high quality and quantity of cysts was obtained when salinity increase started at day-9 of culture.
UDANG ROSTRIS (Litopenaeus stylirostris) KOMODITAS PROSPEKTIF BAGI USAHA PERTAMBAKAN Bambang Sumartono; Joko Sumarwan; Endah Winarni
Jurnal Perikanan Universitas Gadjah Mada Vol 6, No 2 (2004)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.9043

Abstract

Blue shrimp (Litopenaeus stylirostris) known as “SS” (Super Shrimp) originated as wild larvae from Panama, and is currently in its 22nd generation of domestication. They have undergone a systematic process of selection for enhancement of growth and fecundity, and in particular, for resistance improvement to the IHHN virus.The SS-blue shrimp are significantly more tolerant to low water temperatures than Penaeus monodon or Litopenaeus vannamei. This characteristic was effluent to the broodstock management of blue shrimp. The critical phase of larval rearing is on the interchange from zoea to mysis, but almost no mortality on the post larvae stadium.  It was observed during larvae rearing using modulation system indicated by the survival rate of nauplius to PL 5 was 29%, but PL 5 to PL 15 reached 97%.  Growth of larvae and post larvae in the modulation and non modulation systems were not significant different, but performance and uniformity of the seed in modulation system shown better.  Its avarage production in grow out pond was 9,000 kg/ha with final survival rate of 74.5-94.8%, and avarage daily growth (ADG) of 0.176 g. The ponds were stocked with SS-shrimp at densities of 33-38/m2.  
KANDUNGAN DAN KOMPOSISI PIGMEN Sargassum sp. PADA PERAIRAN TELUK AWUR, JEPARA DENGAN PERLAKUAN SEGAR DAN KERING Dian Resita; Windu Merdekawati; AB Susanto; Leenawaty Limantara
Jurnal Perikanan Universitas Gadjah Mada Vol 12, No 1 (2010)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.2897

Abstract

Sargassum sp. is one of seaweed species that abundance and widespread in Indonesia. Besides alginates, Sargassum sp. also contains photosynthesis pigment i.e. chlorophyll a and c, carotene, and fucoxanthin. To date, Sargassum sp. is only used in the form of alginates colloid for industrial raw material with drying process prior to production. Dried process of Sargassum sp. cause pigment degradation especially chlorophyll. The aim of this research was to determine the content and pigment composition of fresh and dried Sargassum sp. Pigment identifi  cation was conducted by Thin Layer Chromatography (TLC), Spectrophotometer and High Performance Liquid Chromatography (HPLC). The contents were analyzed by HPLC. Results showed that Sargassum sp. contain pigment fucoxanthin range from 21.80 to 73.10%, chlorophyll a range from 0.73 to 54.96%, chlorophyll c range from 0.28 to 1.09% and carotene 0.38%. The research results showed that fresh and dry Sargassum sp. contain same pigment composition that were as carotene, pheophytin a, chlorophyll a, chlorophyll c and fucoxanthin.

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