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ILMU KELAUTAN: Indonesian Journal of Marine Sciences

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Published by Universitas Diponegoro

ISSN : 08537291 EISSN : 24067598 DOI : -

Core Subject : Science,

Earth & Planetary Sciences

ILMU KELAUTAN: Indonesian Journal of Marine Sciences (IJMS) is dedicated to published highest quality of research papers and review on all aspects of marine biology, marine conservation, marine culture, marine geology and oceanography.

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Articles 743 Documents

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Pengaruh Dosis Terhadap Efektifitas Vaksin POM Vibrio alginolyticus 74 kDa pada Ikan Kerapu Macan Epinephelus fuscoguttatus Desrina Desrina; Arief Taslihan; Ambariyanto Ambariyanto; Budhi Kuncoro Jati
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 16, No 2 (2011): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Vibrio alginolyticus adalah bakteri patogen penyebab penyakit vibriosis pada ikan kerapu budidaya di Indonesia. Vaksin Protein Outer Membran (POM) V. alginolyticus telah terbukti imunogenik pada ikan kerapu. Penelitian ini bertujuan untuk menentukan pengaruh dosis vaksin terhadap kemampuan POM V. alginolyticus 74 kDa dalam merangsang kerja sistem kekebalan spesifik ikan dan menentukan efek dosis terhadap perlindungan yang dihasilkan. POM(74 kDa) diisolasi dengan metoda sonikasi dan SDS-PAGE, dan dimurnikan dengan metoda elektroelusi. Vaksin diberikan dengan metoda suntik intraperitoneal ke ikan kerapu ukuran 8-10 cm (berat 1013 g) dengan dosis 0 (kontrol), 5, 10 dan 15 µg/0, 1 ml PBS/ 10 g ikan (n= 30 ekor/dosis). Ikan kontrol disuntik dengan 0,1 ml PBS steril. Satu minggu kemudian ikan disuntik booster dengan cara dan dosis yang sama. Dua minggu setelah booster dilakukan uji tantang dengan dengan menyuntikkan bakteri Vibrio alginolyticus 8 secara intramuskular dengan dosis 0,1 ml X 109 sel/ml, dan ikan dipelihara selama 2 minggu. Jumlah ikan yang mati selama masa uji tantang dihitung untuk menentukan Relative Percentage Survival (RPS). Titer antibodi diukur sebelum percobaan dan setiap minggu selama penelitian. Ke 3 dosis yang diberikan efektif dalam merangsang respon kekebalan humoral ikan kerapu dan menghasilkan kekebalan yang melindungi yang hampir sama yang terlihat dari nilai RPS untuk dosis 5, 10 dan 15 µg masing masing 72, 87 dan 72%. Kata kunci: vaksin POM, kerapu, dosis Vibrio alginolyticus is a causative agent of vibriosis of cultured grouper in Indonesia. It has been reported that the Outer Membrane Protein (OMP) of V. alginolyticus vaccine was immunogenic on grouper. Vaccine dose is important in determining the ability of vaccine to conferred protective immunity. The objectives of the present research was to determine effect of vaccine doses on (1) the specific immune response of grouper and (2) conferring protective immunity of grouper. OMP V. alginolyticus (74 kDa) was isolated by sonication and SDSPAGE, and purified by mean electroelution. Vaccine was delivered by intraperitoneal injection to grouper juvenile (8 - 10 cm long and weigh10 - 13 g) in three doses; 0 (kontrol), 5, 10 dan 15 µg/0, 1 ml PBS/ fish (n= 30 fish/ dose). Control fish were injected with 0,1 ml sterile PBS steril. One week later, booster was given in the same manner as the primary vaccination. Two weeks following booster (week 4), fish were challenge with V. alginolyticus 8 by intramuscular injection (0,1 ml X 109 sel/ml) and fish were maintained for two weeks. Fish mortality pos challenge test was counted to calcualte the Relative Percentage Survival (RPS). Antibodi titer was measured before vaccination and weekly for 4 week. All three doses tested were effective to trigger the specific immune response of grouper dan conferred protective immunity with similar degree as shown by the RPS for dose 5, 10 dan 15 µg were 72, 87 dan 72% respectively. Key words: vaccine, OMP, grouper, dose.

Periphyton Response Analysis to the Pollution in Seagrass Ecosystem Panjang Island, Banten Yayuk Sugianti; Barti Setiani Muntalif; Priana Sudjono
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 23, No 3 (2018): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Increases in coastal pollutants, largely due to human activity on land, have an impact on seagrass ecosystems. The high sedimentation in the waters causes an increase in the concentration of sludge, organic matter, nutrients, and turbidity which reduce the depth that can be reached by sunlight. The condition affects seagrass ecosystems adversely. Changes in water condition can be illustrated by the presence of water organisms. One dominant organism in seagrass ecosystems is periphyton. The existence of periphyton in the waters is determined by physical and chemical conditions of the waters because it has specific limit of tolerance, which causes different community structure. To analyze periphyton response to the changes of environmental quality in seagrass ecosystems, Shannon Winner diversity and Saprobic Indices were measured at Panjang Island, Banten. The results of water quality assessment indicates the status of aquatic seagrass of the island considered as polluted to heavy polluted. It is observed from some physico-chemical parameters that exceeded the standard quality for the life of seagrass ecosystems and marine life. Based on the classification and saprobic coefficient using periphyton biological parameters, the condition of seagrass land ecosystem in the island is classified into β Mesosaprobic to β/α Mesosaprobic phase, which indicates light to medium pollution with pollutants including organic and inorganic materials. Several types of dominant periphyton were discovered during the observations, including Meridion sp, Navicula sp, Nitzschia sp and Synedra sp. This periphyton species belong to Bacillariophyceae class (Family Chrysophyta) that is commonly used to assess the condition of eutrophication and organic pollution on waters.

Isolasi dan Identifikasi Bakteri Pendegradasi Koprostanol dari Lingkungan Sungai, Muara, dan Perairan Pantai Banjir Kanal Timur Semarang pada Monsun Timur Misbakul Munir; Norma Afiati; Ocky Karna Radjasa; Agus Sabdono; Tonny Bachtiar
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 9, No 2 (2004): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Aktifitas manusia yang terus meningkat di wilayah pesisir, telah menyebabkan terjadinya peningkatan tekanan lingkungan yang berasal dari berbagai macam sumber pencemaran, antara lain limbah industri, pertanian, transportasi, dan limbah domestik. Salah satu sumber pencemar yang cukup dominan di lingkungan perairan pantai adalah pencemaran akibat limbah domestik. Selama ini yang dipakai untuk mengetahui pencemaran lingkungan oleh limbah domestik adalah menggunakan indikator biologi yaitu bakteri coliform. Namunpenggunaan bakteri coliform sebagai indikator pencemaran limbah domestik mempunyai permasalahan antara lain tidak terdeteksinya bakteri coliform tersebut pada perairan pantai, sementara diduga kuat bahwa perairan tersebut tercemar oleh limbah domestik termasuk feces. Oleh karena itu indikator alternatif sangat diperlukan. Salah satu indikator alternatif pencemaran limbah domestik adalah koprostanol, yang mempunyai sifat cukup konservatif, dapat dikuntitatifkan dan dapat dihubungkan dengan sumber pencemar yang spesifik. Namun perlu diingat bahwa di alam, koprostanol mengalami proses degradasi oleh bakteri, sehingga perlu dilakukan penelitian mengenai isolasi dan identifikasi bakteri pendegradasi koprostanol pada lingkungan sungai, muara, dan perairan pantai Banjir Kanal Timur Semarang pada monsun timur. Penelitian dilakukan pada bulan Juli s/d Agustus 2003 pada lingkungan sungai, muara, dan perairan pantai Banjir Kanal TimurSemarang. Isolasi bakteri dari sampel air dan sedimen dilakukan di Laboratorium Mikrobiogenetika, FMIPA Jurusan Biologi UNDIP Semarang dan identifikasi bakteri pendegradasi koprostanol terseleksi dilakukan diLaboratorium Hama dan Penyakit Balai Besar Pengembangan Budidaya Air Payau (BBPBAP) Jepara. Hasil penelitian diperoleh bahwa bakteri pendegradasi koprostanol yang berasal dari lokasi Banjir Kanal TimurSemarang pada berbagai variasi lingkungan dan habitat diperoleh 69 isolat (83,13%) dari 83 isolat yang diuji. Sedangkan hasil identifikasi terhadap 6 isolat terbaik diperoleh 3 (tiga) genus bakteri mampumendegradasi koprostanol, yaitu Achromobacter, Bacillus, dan Branhamella. Dari 3 genus bakteri pendegradasi koprostanol yang didapatkan, ada 2 (dua) genus yang selama ini belum dilaporkan, yaitu genus Achromobacter, dan Branhamella.Kata kunci : Koprostanol, Limbah Domestik, Indikator Pencemar, Isolasi, Identifikasi, BakteriIncreasing human activities in coastal areas have caused an increase of environmental pressures that come from various sources such as industrial disposal, agriculture, transportation, and domestic wastes. One of dominant sources in coastal waters is contamination by domestic wastes. So far to determine environmental contamination by domestic waste is by using biological indicator, coliform bacteria. However the use ofcoliform bacteria have problems for example, they cannot be detected in coastal waters contaminated by domestic waste including faeces. Therefore, an indicator alternative is urgently needed. Alternative indicatorof domestic waste contamination is coprostanol, which is conservative, easy to quantity and related to specific pollutant source. It is important to note coprostanol is naturally degraded by indigenous bacteria.Therefore it is necessary to conduct a study on isolation and identification of coprostanol-degrading bacteria in river, estuarine, and coastal environments of Banjir Kanal Timur Semarang during dry season. The research had been carried out from July to August 2003 at environmental of river, estuarine, and coastal of Banjir Kanal Timur Semarang. Isolation of bacteria from water and sediment samples were conducted atMicrobiogenetics Laboratory, Faculty of Mathematics and Natural Sciences Diponegoro University, meanwhile identificaton of coprostanol-degrading bacteria selected was conducted by at Pest and Diseases Laboratory,Balai Besar Pengembangan Budidaya Air Payau (BBPBAP) Jepara. The result showed that coprostanol-degrading bacteria obtained from Banjir Kanal Timur Semarang at various environments and habitat were 69 bacterial isolates (83,13%) from 83 isolat tested. Identification revealed that (three) genus were found to degrade coprostanol, namely Achromobacter, Bacillus, and Branhamella. From 3 genus of coprostanol-degrading bacteria identified, 2 of them : Achromobacter, and Branhamella have not been reported so far.Key words : Coprostanol, Domestic Waste, Pollution Indicator, Isolation, Identification, Bacteria

Bioaktivitas Ekstrak Kloroform Metabolit Sekunder yang Dihasilkan oleh Bakteri yang Berasosiasi dengan Karang Lunak Sinularia sp terhadap E. coli, S. aereus, V. harveyi Wilis Ari Setyati; Subagiyo Subagiyo
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 13, No 2 (2008): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Telah dilakukan skrining bakteri yang mampu menghasilkan metabolit sekunder dari karang lunak Sinularia sp, ekstraksi metabolit sekunder serta uji bioaktivitasnya terhadap E. coli, S.aereus, V. harveyi. Pengambilan karang lunak dilakukan di perairan Pulau Panjang dengan metode scuba diving. Bakteri yang berasosiasi dengan karang lunak diisolasi dengan metode spread plate menggunakan media Zobells. Aktivitas antibakteri dari semua isolat yang diperoleh diuji dengan metode difusi agar menggunakan paper disk. Isolat yang menunjukan aktivitas antibakteri tertinggi dilakukan ekstraksi dan fraksinasi terhadap metabolit sekunder yang disekresikan. Ektraksi dan fraksinasi dilakukan dengan metode kromatografi lapis tipis dan kromatograff kolom. Berdasarkan hasil penelitian dapat diskrining 15 isolat bakteri yang berasosiasi dengan karang lunak Sinularia spyang mempunyai kemampuan menghasilkan metaboplit sekunderyang bersifat antibakteri. Hasil uji kuantitatif diperoleh 6 isolat terpilihyang mempunyai aktivitas antibakteri tinggi. Hasil identifikasi menunjukan bahwa isolat-isolat tersebutadalah Vibrio pelagius II, Moraxella, Vibrio anguillarum. Berdasarkan hasil ekstraksi diperoleh 5 fraksiyang berasal dari ekstrak klorofbmn metabolit sekunder dari Moraxella spyangmenunjukan aktivitas antibakteri Fraksi III merupakan fraksiyang paling aktifterhadap E colidan S. aereus, V. harveyi .Kata kunci: Bioaktivitas, ekstrak kloroform , metabolit sekunder, Sinularia sp.

Ekologi Trofik Komunitas Ikan di Perairan Segara Menyan, Subang, Jawa Barat (Trophic Ecology of the Fish Community in Segara Menyan Coastal Lagoon, Subang, West Java) Ahmad Zahid; M.F. Rahardjo; Lenny S Syafei; Rini Susilowati
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 20, No 3 (2015): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Pengetahuan mengenai ekologi trofik merupakan dasar dalam memahami ekosistem secara keseluruhan yang dapat dijelaskan melalui pola hubungan trofik interspesies ataupun interserikat. Penelitian bertujuan untuk menjelaskan ekologi trofik meliputi indeks trofik dan jenis makanan dominan, serikat dan tingkat trofik, dan variasi spasial dan temporal komunitas ikan di estuari Segara Menyan. Pengambilan ikan contoh dilakukan setiap bulan pada zona berbeda. Ikan contoh dipisahkan berdasarkan waktu dan lokasi pengamatan, dianalisis isi saluran pencernaannya. Analisa data meliputi indeks vakuitas, jumlah total organisme makanan, jenis makanan dominan, luas relung makanan, dan tingkat trofik. Pada pengamatan 106 spesies ikan, sebanyak 1-380 saluran pencernaan ikan diamati. Sebanyak lima dari 106 spesies memiliki nilai indeks vakuitas “0” dan jumlah makanan yang dikonsumsi bervariasi mulai dari empat hingga 27 jenis makanan. Secara umum, luas relung ikan adalah rendah berkisar 0,20-0,78 dan kebanyakan berada pada kisaran 0,20-0,48. Zooplankton merupakan jenis makanan paling dominan dikonsumsi oleh ikan. Komunitas ikan dikelompokkan dalam tujuh serikat trofik, yaitu detritivora, fitoplanktivora, zooplanktivora, zoobentivora, moluskivora, krustasivora, dan pisivora. Tingkat trofik komunitas ikan berkisar 2,05-4,73. Faktor perubahan ontogenetik, persediaan makanan, karakteristik habitat, dan ruaya beberapa spesies ikan memengaruhi variasi spasio-temporal jejaring makanan di Segara Menyan. Kata kunci: interaksi trofik, laguna, ikan, variasi spasio-temporal, serikat trofik Knowledge of trophic ecology is one way to understanding the whole ecosystem which explained by trophic relationship pattern (interspecies or interguild). The objective research was described of the trophic ecology, i.e. trophic index and dominant prey, trophic guild and trophic level, and spatio-temporal variation of fish community in Segara Menyan coastal lagoon. Fish were collected monthly for one year at three zones. Fish samples were placed in separate labelled plastic bags according to the time scale and location sampling, then gut contents were analyzed. The data of vacuity index, number of prey, prey dominant, diet breadth, and trophic level were analyzed. For each of 106 fish species, between 1 and 380 stomachs, were examined. The vacuity index was also different among species. Five of 106 species had a vacuity index of “0” and total number of food items consumed varied between fish species, ranging from three items to 27 items. The overall diet breadth (Bi) was relatively low among species, ranging from 0.20 to 0.78, with most of them between 0.20 and 0.48. Calanoid copepods comprised the most common food item consumed by all the fishes examined. Fishes occurring can be broadly categorized into seven different trophic guilds. The trophic level of fish communities ranged from 2.05 to 4.73. Spatio-temporal variation of food web was influenced of ontogenetic shift, food availability, habitat characteristic, and migration of some species in Segara Menyan coastal lagoon. Keywords: trophic interaction, lagoon, fishes, spatio-temporal variation, trophic guild

Pengaruh Kadar Air Terhadap Laju Respirasi Tanah Tambak pada Penggunaan Katul Padi Sebagai Priming Agent Ria Azizah; Subagiyo Subagiyo; Eti Rosanti
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 12, No 2 (2007): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Kualitas tanah tambak merupakan salah satu kunci keberhasilan bubidaya udang, sehingga manajemen pengelolaan tanah tambak mempunyai peran penting dalam menentukan kualitas tanah tambak. Tanah tambak merupakan tempat akumulasi limbah internal tambak yang berasal dari sisa pakan, kotoran udang dan bangkai organisme tambak. Dampak dari bahan organik tersebut dapat dikurangi melalui prinsip ekologi dengan menggunakan tehnik bioremediasi. Penguraian bahan organik dipengaruhi oleh kadar air, sehingga pengaturan kadar air diharapkan mampu meningkatkan laju respirasi tanah tambak. Tujuan dari penelitian iniadalah untuk mengetahui pengaruh kadar air terhadap laju respirasi tanah tambak pada pemberian katul sebagai priming agent. Penelitian menggunakan metode eksperimental dengan rancangan acak lengkap. Perlakuan yang digunakan adalah kadar air 15%, 30% dan 45%, masing-masing dengan pengulangan sembilan kali. Parameter kualitas tanah yang diamati meliputi: total bahan organik, kadar air, N-organik, C-organik dan pH. Berdasarkan hasil pengama tan selama 12 hari menunjukkan bahwa kadar air dapat meningkatkan laju respirasi tanah tambak udang. Laju respirasi tertinggi dicapai oleh perlakuan dengan kadar air 45% sebesar 222,312 mgC/kg tanah per hari, dan disusul berturut-turut oleh perlakuan dengan kadar air 30%, dan perlakuan dengan kadar air 25%, yaitu masing-masing sebesar 215,528 mgC/kg tanah per hari; dan 96,312 mgC/kg tanah per hari.Kata kunci: kadar air, bahan organik, tanah dasar tambak dan laju respirasi.The quality of pond soil are one of the most factor in growth of the shrimp. Pond soil is place of accumulated of pond soil terminal waste are coming from the remained of feed, shrimp exrement and the carrior of the pond soil organism. Waste effect could be decreasing by the ecological principle by using bioremidioation technique. The decomposition of organic matter of bacteria activities extremely influence by moisture. The controlling of moisture could be increasing the respiration rate of pond soil. The purpose of this research is to know the influence of moisture to the respiration rate of pond soil on the use of rice bran as priming agent. The research use experimental method with complete random approach. There are three treatment of moisrureare 15%, 30 % and 45%, nine repeated for each treatment. The taken of soil parameters are the total organic matter, moisture, C-organic, N-organic and pH. According to the observation result during 12 days indicated that the influence of the moisure treatmnet could be increasing the respiration rate of pond soil. In this research the respioration rate from the higgest to the lower are 45% ( 222,312 mgC/kg soil/24 hours); 30% (215,528 mgC/kg soil/24 hours) and 25 % (96,312 mgC/kg soil/24 hours).Key words: Moisture, organic matter, pond bottom soil and respiration rate.

Microplastic in The Bali Strait : Comparison of Two Sampling Methods Defri Yona; Zefanya Nandaningtyas; Bernads Daniel Marolop Siagian; Syarifah Hikmah Julinda Sari; Agung Yunanto; Feni Iranawati; Mochamad Arif Zainul Fuad; Junika Chintia Ayu Putri; Mela Dita Maharani
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 24, No 4 (2019): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Two methods of microplastics sampling in the Bali Strait, manta net (250 µm mesh size) and plankton net (20 µm mesh size), were compared. The difference in the mesh sizes could result in the difference of the microplastics found. Water samples from both sampling tools were analyzed with filtration and all organic materials were removed using Hydrogen Peroxide. Natrium chloride (NaCl) was used to further separate microplastics and organic materials based on its density. The result identified three types of microplastics found in Bali Strait: fibers, films and fragments with total abundances of microplastics were 32.48 x 102 particles.m-3 and 16.33 x 102 particles.m-3using manta net and plankton net, respectively. These results indicated that the numbers of microplastics per cubic metres was higher using manta net sampling tool compared to plankton net. This may likely caused by the smaller size of the mesh used and also the sampling area covered using manta net.

Akurasi Tematik Peta Substrat Dasar dari Citra Quickbird (Studi Kasus Gusung Karang Lebar, Kepulauan Seribu, Jakarta) (Thematic Accuracy of Bottom Substrate Map from Quickbrid Imagery (Case study: Gusung Karang Lebar, Kepulauan Seribu, Jakarta)) Muhammad Banda Selamat; Indra Jaya; Vincentius P Siregar; Totok Hestirianoto
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 17, No 3 (2012): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Salah satu kelemahan metode koreksi kolom air adalah dapat memunculkan bias dalam estimasi rasio koefisien attenuasi. Bias ini berkontribusi pada nilai akurasi tematik peta substrat dasar. Studi ini menggunakan pendekatan zonasi geomorfologi untuk meningkatkan akurasi tematik peta substrat yang dihasilkan dari metode koreksi kolom air. Nilai piksel citra Quickbird dikonversi ke radiansi dan dilanjutkan dengan koreksi kolom air untuk menghasilkan peta substrat dasar dengan tiga tema ekosistem, yaitu ekosistem pantai berpasir dengan substrat dominan pasir, ekosistem lamun dan terumbu karang. Data lapangan dikelompokkan menggunakan metode Bray curtis dan menjadi dasar bagi reklasifikasi. Profil geomorfologi pada citra satelit disadap dari gabungan kanal hijau dan merah, mengacu pada hasil survei batimetri. Pendekatan kombinasi ini terbukti dapat meningkatkan akurasi tematik peta substrat dasar hingga lebih dari 20%.Kata kunci: quickbird, substrat dasar, akurasi tematikBias may occur on attenuation coefficient ratio estimated from water column correction method. This bias then contribute to thematic accuracy of bottom substrate images. This study used geomorphologic spatial zonation to improve thematic accuracy of bottom substrate maps that produced from water column correction method. Quickbird pixel values were converted to the top of atmosphere radiance and followed by water column correction to make bottom substrate map with three themes ecosystem i.e. sandy ecosystem, seagrass ecosystem and coral reef ecosystem. Field data were grouped using Bray Curtis method and become basis of image reclassification. Geomorphological profile was extracted from green and red composite images, refer to a bathymetric survey. These combined approaches were significantly proved to improve thematic accuracy up to more than 20%.Key words: quickbird, bottom subtrate, thematic accuracy

Ekologi Perairan Delta Wulan Demak Jawa Tengah: Distribusi Kepiting (Infra Ordo Brachyura dan Anomura) di Kawasan Mangrove Chrisna Adi Suryono
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 11, No 4 (2006): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Perairan Delta Wulan merupakan salah satu kawasan bermangrove yang masih tersisa dengan baik di wilayah Pantai Utara Jawa Tengah. Daratan delta tersebut dimanfaatkan sebagai tambak baik ikan, udang maupunkerang. Banyak organisme yang berasosiasi dengan mangrove salah satunya adalah kepiting. Tujuan dari penelitian ini adalah untuk mengetahui distribusi kepiting di kawasan mangrove tersebut. Pengambilan sampel kepiting dilakukan di kawasan mangrove dengan luasan 5x5 m pada 4 stasiun yang berbeda. Hasil pengamatanditemukan 12 jenis kepiting dan 9 jenis mangrove yang terdistribusi di keempat stasiun. Keduabelas jenis kepiting hampir tersebar di keseluruhan stasiun, jumlah individu terbanyak di temukan pada stasiun I yang lokasinya dekat laut sedangkan yang terkecil pada stasiun IV yang lokasinya jauh dari laut. Pola sebaran kepiting pada masing masing stasiun adalah mengelompok dan komunitas kepiting pada stasiun II, III dan IV memiliki kesamaan yang tinggi diatas 90%.Kata kunci : Delta Wulan, kepiting, mangrove. Delta Wulan waters is one of the mangrove areas in North Coast of Central Java. Most of deltas are functioned as pond to cultivate fish, shrimp and cockles. Many of animals were associated with mangrove vegetation to make simbiont one of them is crab. The aims of the research were to understand the distribution of crabs on mangrove areas. The samples were collected in 5x5 square meter in mangrove areas in 4 different stations. The result of the research showed that there were 12 species of crabs and 9 species of mangroves which distribute on 4 stations. Most of the crabs and mangroves were distribute on 4 stations and the highest number of crabs was found at station I which closer to the beach and the lowest number was found at station IV which is further away from the beach. The dispersal pattent of crab in their location was clumped and the stations II, III and IV have highest community similarity index with the number more than 90%.Key words: Delta Wulan, crab, mangrove.

Genetic Profile Assessment of Giant Clam Genus Tridacna as a Basis for Resource Management at Wakatobi National Park Waters Muhammad Nur Findra; Isdradjad Setyobudiandi; Nurlisa Alias Butet; Dedy Duryadi Solihin
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 22, No 2 (2017): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Giant clam population has been decreased in a few years. Resource management requires information from various aspects, such as ecological, population, and other aspects. This study was aimed at assessing the genetic profile of Tridacna giant clam in Wakatobi National Park waters using Cytochrome oxidase subunit I (COI) genetic marker. Sample collection was conducted around the three main islands, i.e., Wangi-wangi, Kaledupa, and Tomia. Genetic analysis using COI gene may contribute in identifying giant clams up to the species level and showed the relationship among species. The research found 41 specific nucleotide sites for the clams. T. crocea, T. squamosa and T. maxima had 2, 15 and 24 sites, respectively. COI gene as a biological marker was able to separate groups of giant clam by species. Nucleotide variation of T. crocea from Wakatobi was the highest among other locations, so it could be used as a genetic source for translocation and domestication. Keywords: cytochrome oxidase subunit I, specific nucleotide, Tridacna, Wakatobi National Park

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