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Biosaintifika: Journal of Biology & Biology Education

biosaintifika
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Published by Universitas Negeri Semarang

ISSN : 2085191X EISSN : 23387610 DOI : -

Core Subject : Agriculture,

Agriculture, Biological Sciences & Forestry

Biosaintifika Journal of Biology & Biology Education, an electronic international journal, provides a forum for publishing the original research articles, review articles from contributors, and the novel technology news related to biological research and biological education.

Arjuna Subject : -

Articles 1,068 Documents

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Profile of SGPT and SGOT on Male Rats (Rattus norvegicus) Hyperglycemic After Giving Insulin Leaf Extract (Tithonia diversifolia) Yuneldi, Rizki Fitrawan; Saraswati, Tyas Rini; Yuniwarti, Enny Yusuf Wachidah
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 3 (2018): December 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

The leaves of insulin (Tithonia diversifolia (Hemsl.) A. Gray) are native bush plants from Central America. This plant in Indonesia is often called paitan or kembang bulan. The aim of this research was to know the profile of SGPT and SGOT of male white rat (Rattus norvegicus L.) hyperglycemic after giving insulin leaf extract (Tithonia diversifolia (Hemsl.) A. Gray). This study used 20 male Wistar rats which were divided randomly into 5 groups of treatment. Those were P0 as a group of rats which was not given alloxan and insulin leaf extract, P1 as a group of rats which was given alloxan without insulin leaf extract, P2 as a group of rats which was given alloxan and glibenclamide 2 mg/Body Weight (BW)/day, P3 as a group of rats which was given alloxan and insulin leaf extract 30 mg/BW/day, P4 as a group of rats which was given alloxan and insulin leaf extract 60 mg/BW/day. Each treatment was repeated as many as 4 replications. The results of this study indicated that there was no significant differences in all variables, namely levels of SGPT, SGOT, liver weight, body weight and Hepatosomatic index (HSI), so it can be concluded that giving insulin leaf extract (Tithonia diversifolia (Hemsl.) A. Gray) of 30 mg/BW/day and 60 mg/BW/day could improve liver function of hyperglycemic male white rats (R. norvegicus L.).

Phylogenetic Analysis of Polyporous Fungi Collected from Batam Botanical Garden, Riau Province, Indonesia Lestari, Anis Sri; Zulfiana, Deni; Zulfitri, Apriwi; Krishanti, Ni Putu Ratna Ayu; Kartika, Titik
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 3 (2018): December 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Botanical gardens are areas that provide protection for trees and other organisms like polyporous fungi. Polyporous fungi are important fungi that degrade remaining lignocellulosic in leaf litter or dead trees. These mycobiota are also noted for their vital role in biorefinery, bioremediation, medicine and phytopathogen. The knowledge of the importance of the polyporous fungi to describe polyporous fungal species is fundamental for generating data base information of their occurrence and their functions. This research’s goal was to explore and characterize the polyporous fungi collected in Batam Botanical Garden in three sampling areas. Fungal samples were collected in May and July 2017. Subsequently, morphological characters were recorded, the fungal tissue was isolated to extract the DNA, then the data sequence was amplified and aligned to construct a phylogenetic tree. Five fungal families found belong to order Polyporales and were classified morphologically. They were Polyporaceae, Ganodermataceae, Fomitopsidaceae, Irpicaceae and Hymenochaetaceae. Three fungal species namely; Pycnoporus sanguineus, Trametes ijubarskii, and Antrodia wangii were identified based on phyllogenetic analysis whereas seven other fungal samples were identified as Earliella scabrosa, Hexagonia tenuis, Polyporus tenuiculus Lenzites betulina, Lentinus concavus, Phellinus rimosus and Hexagonia apiaria. This study classifies and adds fundamental databases on fungal taxonomy and diversity on the fungal organisms found in Batam Botanical Garden. This background data is vital to carry out an advance research in some areas such as bio-chemistry, bio-degradation, pharmacology and biotechnology.

Sex Determination in Male and Female Melopsittacus undulates using a Morphometric Method Baehaqi, Ibnu; Saraswati, Tyas Rini; Yuniwarti, Enny Yusuf Wachidah
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 3 (2018): December 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Parakeets (Melopsittacus undulates) are classified parrot order Psittaciformes. Parakeets are monomorphic birds whose sex is difficult to be distinguished. Sex identification is very important for breeding efforts in order to increase the parakeet population. External morphology was determined to identify the sex of the birds. This research was conducted to determine the sex of parakeets (male and female). This study used five male and five female parakeets aged 4 months as the study objects. This study used the quantitative and qualitative method. Results of this study was verified by performing surgery to determine the sex of parakeet based on their reproductive organs. The t-test results of morphometric characteristics showed no significant different in the length of body, upper bill, lower bill, wing, tail, femur, tibial-tarsus, tarsometatarsus and digits as well as the body weight between male and female parakeets. The color of the cere was useful to accurately determine sex in parakeets (blue in males and white in females). This research provided information to the public about the differences between male and female parakeets for the selection of good broodstock in order to increase their population in captivity.

Potential of Chemolitotrophic Bacteria From Gold Mining Area in Sulfur Oxidation Process Fitriyani, Nur Lu'lu; Irianto, Agus; Pramono, Hendro
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 3 (2018): December 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Gold in nature is covered by rocks which contain sulfide minerals such as pyrite, chalcopyrite, arsenopyrite, and others sulfide minerals. Chemolithotrophic bacteria have the ability to oxidize the sulfur compounds and can be used in the process of releasing gold from carrier rocks which contain sulfide minerals. This research aimed to explore and identify the chemolithotrophic bacteria from gold mining areas as well as determine their potential for sulfur oxidation. The methods used in this study were exploring the potential of bacteria in sulfur oxidation and describing the variety of bacteria that were isolated from gold mining areas by 16s rRNA identification. The results showed that there were six isolates from isolation with Starkey solid medium, i.e. Bl-1, B1-2, B1-3, B1-4, B1-5 and B1-6 that were similar to Paenibacillus sp., Enterobacter ludwigiis train E8-13, uncultured Burkholderia sp., Uncultured bacterium clone N4.5, Bacillus subtilis strain CICC 10023, and Bacterium enrichment culture clone 02 respectively. The B1-3 isolate showed the highest increase of sulfate compound in the medium (8.04 % at 649.55 ppm). This indigenous bacteria will be able to be used to release gold from rock which contains sulfide minerals and reduce the use of hazardous chemicals commonly used in gold mining.

Home Range Estimation and Food Plants Preference of Presbytiscomata at Situ Patengan Nature Reserve Widiana, Ana; Januari, R. Robbi; Hasby, Rizal Maulana; Yuliawati, Astri
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 3 (2018): December 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Surili (Presbytis comata) is one of endemic primates of West Java whose existence is protected. The purpose of the research was to determine the estimated home range and the preferences of food plant of P. comata at Situ Patengan Nature Reserve, West Java. The home range was determined by the minimum convex polygon method by connecting the coordinates of the outermost entire outer area groups. Focal animal sampling and Ad-libitum methods were applied to obtain data of food preferences in details. The result showed that home range of several P. comata groupsat Situ Patengan Nature Reserve were varied. The home range of group A, B, C, and D were covering 3.52 Ha, 4.43 Ha, 3.76 Ha, and 3.14 Ha respectively, while the solitary individual has a home range covering 2.64 Ha. There were 27 species from 16 families of plants that was directly consumed by P. comata. P. comata more often consumed Castanopsis javanica (10.07%), C. argentea (9.35%), C. tungurut (7.91%), Sloneasigun (7.91%), and Quercus sp (7.19%). This data can be useful as a reference in P. Comata population and habitat management especially in the area of Situ Patengan.

Growth and Survival Evaluation of Oreochromis Sp fed Hermetia illucens Larva and Manihot esculenta leaves Meal Aini, Nur; Nugroho, Rudy Agung; Hariani, Nova
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 3 (2018): December 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

This study was conducted to compare the effects of dietary substitution of fish meal (FM) with black soldier fly (Hermetia illucens) larvae meal (BSFM) and Manihot esculenta leaves meal (MEM) on the growth and feed efficiency of Oreochromis sp. Four concentrations viz: P1 (25%): 50g BSFM and 25g MEM, P2 (50%): 100g BSFM and 50g MEM, P3 (75%): 150g BSFM and 75g MEM, P4 (100%): 200g BSFM and 100g MEM were prepared and tested against control without FM replacement. Each diet was fed to three replicates groups of fish at a rate of 5% of body weight two times per day for 30 days. At the end of the trial, growth parameters, Feed conversion rate (FCR), and feed efficiency (FE) were evaluated. The results showed that fish fed dietary substitution of FM with combination ratio of BSFM and MEM higher than 50% significantly improved all growth parameters, FCR and FE. It is therefore suggested that the partial (higher than 50%) or total replacement of fish meal with combination of BSFM and MEM in the diet of Oreochromis sp can be used as fish meal substitution to obtain better growth and feed efficiency.

Practical Guide Based on Learning Cycle 5E to Enhance Students’ Cognitive Learning Outcomes Pangestika, Dwi Safriani; Prasetyo, Zuhdan Kun
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 3 (2018): December 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

The practical guide is a teaching material that students use in labwork activities. This learning cycle 5E (Engangement, Exploration, Explanation, Elaboration, Evaluation) on practical guide is expected to be used as an alternative choice for teacher in improving students’ cognitive learning outcomes in concept of pressure and symptoms of capillarity in plants. The aim of this study was : 1) to describe the feasibility of develop practical guide based on learning cycle 5E and 2) to analyse the improvement of the practical guide based on learning cycle 5E to enhance cognitive learning outcomes of eight graders of junior high school in science lesson. Here, we used the Research and Development refers to the procedural steps of the 4D models by Thiagarajan. To test the efficacy, pretest and posttest were employed.This is a quasi-experiment research conducted in MTsN 3 Ngawi. Research subjects were all students of grade 8 by taking class 8A as the experimental class and 8B as the control class. Samples were taken by cluster sampling technique. The result of the feasibility test showed that practical guide included in the excellent category. T-test analysis suggests that the practical guide based on learning cycle 5E could improve students’ cognitive learning outcomes significantly. In addition to contributing to the improvement of students’ cognitive learning outcomes in MTsn 3 ngawi, the development of science cycle-based learning guide based on learning cycle 5e can also provide students practice skills in learning so that it is expected to contribute posotif in the community.

Diversity and Abundance of Orchids at Gebugan Nature Reserve in Semarang, Indonesia Farokhah, Tria; Utami, Sri; Jumari, Jumari
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 2 (2018): August 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i2.14579

Orchid is a plant with a high aesthetic value.The existence of orchids directly by the community from their natural habitat, causing the existence of orchid in nature was threatened. The potential of orchid diversity in the Gebugan Nature Reserve area needs exploration to maintain its sustainability. This research would explore the diversity and abundance of orchid species and determined the environmental conditionof Gebugan Nature Reserve. The study was done on 2 stations with altitude difference are 900 m asl and 1045 m asl. Five plots of 10 m x 10 m were systematically constructed at each station with a distance of plots 50 meters. Species of orchids found in the Gebugan Nature Reserve were 12 species, including 11 species of epiphytic orchids and 1 species of terrestrial orchids. The abundance of individual orchid species was relatively higher in places with higher altitudes. Micropera sp was the most abundant orchid species and Corymborkis veratrifolia was the species of orchid with the lowest abundance. It is found that the environmental conditions of Gebugan Nature Reserve are suitable for orchid. The novelty of the research is providing a new data base of orchid in the Gebugan Nature Reserve. The result of the study would be beneficial for developing strategy of genetic conservation of orchird germplasm.

Utilization of Snakehead Fish Meat for Increasing Kidney Function in Rat Experiencing Physiological Stress Sunarno, Sunarno; Sokmawati, Hajar; Rahadian, Rully
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 3 (2018): December 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Physiological stress for a long time can lead to impaired kidney organ function. Snakehead fish meat are known to contain important nutrients such as albumin, glutamine, glycine, cysteine, and various minerals that can improve kidney function. This study was conducted to examine and analyze the effect of snakehead fish supplementation in feed towards body weight, kidney weight, diameter and number of glomerulus in Wistar rats with physiological stress. This study used 20 male Wistar rats divided into 5 groups consisted of 1 control group (P0) and 4 treatment groups (P1: 5%, P2: 10%, P3: 15%, P4: 20%). The treatments were performed with 4 times repetition. This study used Completely Randomized Design (CRD). The variables measured were body weight, kidney weight, diameter and number of renal glomerulus. The data obtained were analyzed using Analysis of Variance (ANOVA) at 5% significance level and continued with Duncan test with 5% significance level. The results of this study were: the supplementation of snakehead fish with 20% of concentration can increase the body weight; concentration 10; 15; and 20% can increase the weight of the kidney; concentrations of 5%, 10%, 15%, and 20% can increase the glomerular diameter in Wistar rats with physiological stress. The supplementation of snakehead fish with concentration of 5% to 20% did not affect the number of glomerulus of Wistar rats with physiological stress. Results of this study is useful as information for people who study the mechanism of kidney repair due to physiological stress.

Endophytic Bacteria from Faloak Plant Seed (Sterculia comosa) as Antibacterial Agent Moi, Maria Yasinta; Kusdiyantini, Endang; Pujiyanto, Sri
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 3 (2018): December 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Endophytic bacteria isolated from some various kind of plants are able to yield some active compounds which have a role as an antibacterial compound. This work aimed to isolate and to screen the Endophytic bacteria from Faloak seed in its charge in inhibiting two kinds of pathogenic bacteria, Staphylococcus aureus and Escherichia coli. There were six isolates of Endophytic bacteria isolated in this work. According to the screening result, one isolate which had the most potential antibacterial activity (marked by the formation of inhibition zone) against S. aureus and E. coli. That most potential isolate was then tested and identified for both biochemical properties and molecular 16S rRNA gene. The result of this study showed that the endophytic bacteria isolate of Faloak seed with the code of S1 had the similarity with Enterobacter xiangfangensis strain 10-17 by 93 %. The research about endophytic bacteria of Faloak plants was never conducted before. Thus this research was expected to give information about the potential of antimicrobial ability Faloak plants which can be utilized in the discovery of new antibiotic compounds which in the future are expected to overcome the problem of microorganism resistance to antibiotics. The use of endophytic bacteria is expected to prevents the extinction of Faloak plants due to excessive use.

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Dr. Ir. Dyah Rini Indriyanti

Contact Email
biosaintifika@mail.unnes.ac.id

Phone
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Journal Mail Official
biosaintifika@mail.unnes.ac.id

Editorial Address
-

Location
Kota semarang,

Jawa tengah

INDONESIA