cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
Dr. Ir. Dyah Rini Indriyanti
Contact Email
biosaintifika@mail.unnes.ac.id
Phone
-
Journal Mail Official
biosaintifika@mail.unnes.ac.id
Editorial Address
-
Location
Kota semarang,
Jawa tengah
INDONESIA
Biosaintifika: Journal of Biology & Biology Education
Published by Universitas Negeri Semarang
ISSN : 2085191X     EISSN : 23387610     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Biosaintifika Journal of Biology & Biology Education, an electronic international journal, provides a forum for publishing the original research articles, review articles from contributors, and the novel technology news related to biological research and biological education.
Arjuna Subject : -
Articles 1,068 Documents
 27   28   29   30   31 
Characterization of Three Species of Thrips on Weeping Fig, Nutmeg, and Marine Seruni Plants Based on Mtcoi DNA Sequences Kurniawaty, Nia; Hidayat, Purnama; Rauf, Aunu
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 2 (2016): September 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i2.5448

Abstract

Thrips are widely reported as pests in vegetable crops. However, the existence of Phlaeothripidae members has a less concern in Indonesia. Phlaeothripidae is the only family of Tubulifera Suborder and some reports suggested that they had potential to be pests in several crops due to their ability to roll up and to make galls on leaves. The first step in pest management attempt is to identify the pest accurately and quickly, so the pest management can be on target and more efficient. One of the identification methods is the molecular identification using DNA barcoding techniques. This study aimed to characterize and to compare species thrips in banyan, nutmeg, and marine seruni based on their molecular characteristics. This research was conducted in Bogor and Kuningan. The process of molecular characterization consisteds of four steps DNA total extraction, amplification by using PCR, COI gene sequence, and data analysis. PCR programme was succesfully to amplified mtCOI gene fragment at 710 bp. The length of mtCOI gene of Gynaikothrips uzeli, Haplothrips ganglbaueri, and Pseudophilothrips ichini were 704, 686, and 702 bp dominated by A and T bases with nucleotide variation value of 27.8%. This results confirmed that molecular characterization using mtCOI gene mitochondrial had successfully supported the morphological data.How to CiteKurniawaty, N., Hidayat, P. & Rauf, A. (2016). Characterization of Three Species of Thrips on Banyan, Nutmeg, and Marine Seruni Plants Based on Coi Gene. Biosaintifika: Journal of Biology & Biology Education, 8(2), 185-192.
Development of In Vitro Conservation Medium of Carica pubescens Lenne & K. Koch through Nutrients Concentration Reduction and Osmoregulator Addition Rahayu, Enni Suwarsi; Habibah, Noor Aini; Herlina, Lina
Biosaintifika: Journal of Biology & Biology Education Vol 7, No 1 (2015): March 2015
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v7i1.3538

Abstract

Carica pubescens Lenne & K. Koch is a rare species that need to be conserved. The research aim was to develop a slow growth method of in vitro conservation medium through determining some effects of nutrition decreasing availability in the conservation medium on growth and survival of explants. Establishing epicotyls reached from in vitro seed germination was grown on diluted basic medium of 75% MS (Murashige and Skoog), 50% MS, 25% MS, while osmoregulator compound of mannitol and sorbitol was added to the full MS medium in several concentrations. The treatments were arranged in a completely randomized design with three replications. The epicotyls were grown at storage medium for 12 and 16 weeks, then their survival were evaluated at regeneration medium and rooting medium. The diluted basic medium and osmoregulator addition were evaluated for its influence in retarding the culture growth in terms of improved survival over the period of 16 weeks. Data analyzed by one way analysis of variance and Duncans multiple range test. The results showed that the decreasing of nutrition concentration suppressed the growth of the epicotyls until 16 weeks after conservation. Epicotyls taken from 16 weeks after conservation could grow on the regeneration medium. The best survival was shown by the 75% MS, 50% MS and supplementing of 20 g/l mannitol treatments. Based on these results, 50% MS medium is recommended for storage C. pubescens for 16 weeks with no sub-culture.Carica pubescens Lenne & K. Koch (karika dieng) merupakan tanaman yang langka sehingga perlu dilestarikan. Penelitian ini bertujuan untuk memperoleh medium penyimpanan in vitro dengan teknik pertumbuhan minimal dengan mengamati pengaruh penurunan ketersediaan nutrisi dalam medium terhadap penurunan pertumbuhan dan daya tumbuh eksplan.Eksplan berupa epikotil kecambah in vitro. Perlakuan penurunan ketersediaan nutrisi dilakukan melalui reduksi konsentrasi nutrisi dari medium Murashige & Skoog (MS) dan penambahan osmoregulator (manitol dan sorbitol) dengan berbagai konsentrasi. Penelitian dilakukan dengan rancangan acak lengkap satu faktor dengan tiga ulangan. Epikotil dipelihara dalam medium penyimpanan selama 12 dan 16 minggu, kemudian dievaluasi daya tumbuhnya dengan memelihara dalam medium regenerasi dan medium pengakaran. Data dianalisis dengan analisis varians satu arah dan uji Duncan. Hasil penelitian menunjukkan bahwa penurunan kecepatan penyerapan nutrisi berpengaruh terhadap pertumbuhan eksplan. Epikotil yang telah disimpan selama 12 minggu dan 16 minggu dan ditumbuhkan kembali pada medium regenerasi masih dapat tumbuh dengan intensitas tertinggi pada perlakuan pengenceran 50% MS dan 75% MS, serta penambahan manitol 20 g/l. Komposisi medium ini dapat dimanfaatkan untuk penyimpanan karika dieng selama 16 minggu tanpa sub-kultur. Konsentrasi medium MS 50% direkomendasikan untuk digunakan dalam penyimpanan C. pubescens selama 16 minggu tanpa sub-kultur.
Resistance of Advanced Soybean Lines to Pod Borrer (Etiella zinckenella) Kuswantoro, Heru; Bayu, Marida S. Y. I.; Baliadi, Yuliantoro; Tengkano, Wedanimbi
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i2.7895

Abstract

The increasing and stabilizing of soybean product in Indonesia face many limitations. One of the limiting factors is pod borrer (Etiella zinckenella Treitschke) infestation that is able to cause yield loss up to 80%. Objective of the research was to find out some advanced soybean lines that resistant to pod borrer. Design was randomized complete block with three replications. Soybean lines were grown gradualy to ensure the simultanously flowering. The plants were caged at 35 days after planting (DAT) and infested with the imago of E. zinckenella at 56 DAT. Results showed that different soybean lines affected imago population, eggs population, larvae population, infected pods and infected seeds. Some genotypes were consistantly resistant to E. zinckenella. The resistance of those genotypes were non preference resistance based on eggs population, larvae population, infected pod and infected seeds. This study discovered nine soybean lines that is resistant to E. zinckenella, so that it can be beneficial for improving soybean resistance to this pest through releasing as a new resistant pod borer variety after tested further in potential yield and genetic x environment interaction trials. In addition, there were three varieties and two germplasm accessions that can be used as gene sources for improving the resistance of the varieties. The three varieties are able to be cultivated directly in field to decrease the E. zinckenella occurrence.
Hematology and Blood Chemistry Status of Most Frequently Consumed Ruminants in Community Tana, Silvana; Saraswati, Tyas Rini; Yuniwarti, Enny Yusuf
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 2 (2018): August 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i2.12714

Abstract

Hematological and chemical status of blood in livestock can be used to diagnose the disruption of the hematologic system as well as help to diagnose many organ and systemic diseases. This study aimed to determine the hematology and blood chemistry status of various types of most frequently consumed ruminants in community. Types of ruminants used were cattle, goats and buffaloes. The research design was Completely Randomized Design (RAL) with 3 treatments on 3 types of male ruminants at slaughter age. Each treatment consisted of 3 times repetition. The animals used came from slaughterhouses in Kudus (cattle and buffaloes) and Semarang (goats). Observations of hematology and blood chemistry parameters were conducted on blood samples taken in the morning (at 03.00 a.m.). The parameters measured were the number of erythrocytes, hemoglobin (Hb) content, number of leukocyte and blood chemistry consisted of HDL and LDL cholesterol. The data obtained were analyzed by ANOVA and followed by Duncan test. The results showed that the highest number of erythrocytes and hemoglobin content was found in goats, whereas the highest LDL content was found in cattle. It can be concluded that goat has the best hematological status compared to cattle and buffalo.
Characteristics of Cells from Five Exotic Bamboos after Drywood Termites Incisitermes minor (Hagen) Attack Subekti, Niken; Yoshimura, Tsuyoshi
Biosaintifika: Journal of Biology & Biology Education Vol 7, No 2 (2015): September 2015
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v7i2.3952

Abstract

This study examined the relationship between and the characteristics of five bamboos exotic in the cell walls. For this purpose, the bamboosexotic from Indonesia were separated into cells after drywood termites Incisitermes minor (Hagen) attack. SEM observations revealed that bamboos cells. Moreover, SEM analysis showed that the cellulose isolated from parenchyma cells. These results suggest that all the cellulose micro?brils in five bamboos different characteristics of cell function after drywood termite attack.Penelitian ini menguji hubungan antara karakteristik dan lima bambu eksotis di dinding sel. Untuk tujuan ini, bamboosexotic dari Indonesia dipisahkan ke dalam sel setelah rayap Drywood Incisitermes minor (Hagen) serangan. Pengamatan SEM menunjukkan bahwa sel-sel bambu. Selain itu, analisis SEM menunjukkan bahwa selulosa yang diisolasi dari sel parenkim. Hasil ini menunjukkan bahwa semua brils fi selulosa mikro di lima bambu karakteristik yang berbeda dari fungsi sel setelah serangan Rayap Kayu Kering.
Analisis Keanekaragaman Sagu (Metroxylon sagu Rottb.) pada Tiga Tipe Habitat di Pulau Padang Kepulauan Meranti Rahayu, Yeni; -, Fitmawati; -, Herman
Biosaintifika: Journal of Biology & Biology Education Vol 5, No 1 (2013): March 2013
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v5i1.2569

Abstract

Pulau Padang memiliki areal penanaman sagu (Metroxylon sagu Rottb.). Menurut pengetahuan masyarakat lokal terdapat tiga tipe habitat kebun sagu di Pulau Padang, yaitu Gambut, Kilang Manis, dan habitat bertanah liat. Produktifitas sagu berkorelasi dengan ketiga tipe habitat tersebut. Tujuan penelitian ini adalah mengungkap keanekaragaman sagu pada tiga tipe habitat di Pulau Padang dan menentukan tipe habitat yang mendukung produktifitas sagu yang tinggi, sebagai informasi dasar bagi upaya konservasi habitat sagu. Sebanyak 19 individu sagu telah dikoleksi dari tiga tipe habitat dan diamati karakter morfologi dan agronominya. Hasil karakterisasi ditemukan tiga tipe variasi sagu yaitu sagu duri, sagu sengke, dan sagu bemban. Analisis kluster menunjukan bahwa tanaman sagu mengelompok pada tiga kelompok utama berdasarkan asal habitat dan keberadaan durinya pada tingkat kesamaan 31-88%. Berdasarkan analisis komponen utama membagi tanaman sagu menjadi 5 kelompok, cenderung mengelompok juga berdasarkan asal habitat dan keberadaan duri dengan nilai akumulasi keragaman dua komponen utama 58%. Tipe habitat kilang manis direkomendasikan sebagai habitat sagu yang perlu dikonservasi di pulau Padang.Padang Island has a large sago plantation areas. Indigeneous people recognize three habitat types of sago in Padang island, i.e. peat, kilang manis and clay soil. The productivity of sago is closely correlated to the habitat types. The aims of this research were to reveal the diversity of Sago palms in three habitat types in Padang Island and to determine the habitat types which support high productivity of sago as the information base for conservation of sago habitats. The nineteen sago individuals were collected from three habitats and their morphological and agronomic characters were observed. The result of sago characterization obtained three variations of sago in Padang Island, namely Duri, Sengke and Bemban. The cluster analysis showed that sago dendrograms, which was grouped into three main groups based on similarities of origin and the presence of spines with 31-88% similarity coefficient. The principal component analysis classified the diversity of sago into five groups with 58% accumulated value of diversity. Furthermore, Kilang manis, which in recent study is known as a part of Peat habitat, needs to be conserved for sago plantation area.
Plantlets Regeneration from Crown Bud Slicing of Pineapple (Ananas comosus) Zulkarnain, Zulkarnain; Neliyati, Neliyati; Eliyanti, Eliyanti
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 3 (2018): December 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (323.82 KB) | DOI: 10.15294/biosaintifika.v10i3.8079

Abstract

Pineapple propagation by lateral shoots, suckers or crowns is often confronted with limited number of regenerated seedlings and high diversity in flowering and fruit formation. In order to solve this problem, this study offer an alternative method by using tissue culture techniques. This study aimed to determine the effect of growth regulators on plantlet regeneration from bud slicing of pineapple cv. Tangkit. Four levels of 2.4-D (0.0, 0.001, 0.01 and 0.1 ppm) in combination with BA (0.0, 0.1, 1.0 and 10.0 ppm) were tested on solid MS medium. Cultures were incubated in total darkness for a week followed by transfer to 16-hour photoperiod. Results showed that explants treated with 2,4-D and/or BA succeeded in regenerating adventitious shoots. Average leaf number did not differ significantly among treatments (P = 0.60). Highest leaf number (2.99 ± 0.23) was obtained on medium with 0.01 ppm 2,4-D without BA, followed by 0.1 ppm 2,4-D without BA (2.85 ± 0.33). Meanwhile, roots were only formed on medium with 0.1 ppm 2.4-D without BA (4.2 ± 0.37 per shoot). Thus, complete plantlets were regenerated only on medium supplemented with 0.1 ppm 2,4-D without BA. The growth of plantlets was relatively uniform, and plantlet acclimatization succeeded 100% on Jiffy pots. The finding of optimum concentration of 2.4-D and BA in this study is important to develop standard protocol for in vitro propagation of pineapple cv. Tangkit. Thus, the benefit of producing seeds in large quantities and relatively uniform in growth is made possible through tissue culture technique.
Relationship of Bird Diversity and Plant Composition Inside The Area Campus Green Space of Universitas Padjadjaran Jatinangor, Sumedang West Java Nurjaman, Deden; Husodo, Teguh; Megantara, Erri Noviar; Hadikusumah, Herri Y.; Wulandari, Indri
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 3 (2018): December 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (287.747 KB) | DOI: 10.15294/biosaintifika.v10i3.13543

Abstract

Padjadjaran University (UNPAD) Jatinangor is currently conducting green Campus program. To support the program, a study of biota living in it, as one of the benchmarks of good or bad environmental conditions, is needed. The green space of Jatinangor Campus is divided into two clusters namely Cluster I green space (Campus Forest) and green space Cluster II (Campus Non Forest). The objective of the research was to know the relationship between diversity of birds with diversity of plants in the green space of Cluster I (Campus Forest) and Cluster II (Campus Non Forest) UNPAD Campus Jatinangor as one of the parameters of successful development of green Campus. This research is descriptive-explorative with census method on bird species and plant composition from green spaces of Cluster I (Campus Forest) and Cluste II (Campus Non Forest) Campus UNPAD Jatinangor. From the observations in Cluster I, we identified 46 species of birds and 77 species of plants, whereas in Cluster II, we identified 32 species of birds and 74 types of plants. The number of bird species is directly proportional to the number of plant species from Cluster I and Cluster II green spaces. From this study it was concluded that the diversity of tree species supports the diversity of bird species.
Effect of Ethanol and IPTG on the Recombinant Jembrana Trans-Activator of Transcriptation Protein Expression Indriawati, Indriawati; Salfia, Mega; Susanti, R; Margawati, Endang Tri
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 3 (2018): December 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (526.116 KB) | DOI: 10.15294/biosaintifika.v10i3.15596

Abstract

Jembrana diseases are caused by Jembrana Diseases Virus (JDV). The previous study showed that Jembrana Trans-Activator of Trancriptation (JTAT) recombinant protein is effective as a vaccine for Jembrana diseases. The production of JTAT protein needs to be optimized to obtain a higher amount of vaccine. High expression of JTAT protein will produce a high vaccine product. This study aimed to examine the effect of the addition of ethanol and IPTG in E. coli media on the expression of JTAT recombinant protein. This research was experimental research with factorial RAL design with a variation factor of ethanol and IPTG. Qualitatively, the induction of each IPTG, ethanol and interaction between the two could induce the expression of JTAT protein and could be identified with SDS-PAGE at ±11.8 kDa. Statistically, the induction of IPTG, ethanol and interaction between the two were not significantly different. Qualitative and quantitative data show that ethanol can induce JTAT protein expression. This result can be used as a preliminary study to test the effectiveness of ethanol as a substitute for IPTG in inducing the recombinant protein expression.
Predicting Invasion Probability from Botanic Gardens using Exotic Species Traits Junaedi, Decky Indrawan; Mutaqien, Zaenal
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 3 (2018): December 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (304.632 KB) | DOI: 10.15294/biosaintifika.v10i3.15500

Abstract

Preventative management, such as framework-based assessment, considered as the best option for invasive species management. Alternatively, risk assessment can be conducted based on traits of occurred invasive species to build prediction system for invasive risk assessment. This study aimed to test whether trait-based assessment system can differentiate the escaped from non-escaped exotic collections of botanic gardens and to compare the reliability of trait-based versus framework-based risk assessment on differentiating these escaped from non-escaped exotics. In this study, Bayesian logistic regression analysis was conducted to assess the reliability of framework-based and trait-based risk assessment systems. For trait-based system, clear effect of leaf trait, height, and dispersal method to escape probability was detected. For framework-based system, clear effect of Tropical Weed Risk Assessment Protocol on escape probability was detected. Leaf trait, dispersal method and height are reliable predictors for escaped probability of botanic gardens exotic collection. The fact that the reliability of trait-based assessment systems is better than the commonly used framework-based system is the main novel finding in this study. This finding implies that trait-based is better than framework-based for invasive species risk assessment approach in Indonesian botanic gardens. Trait-based assessment also a relevant tool to support management with limited resources to conduct adequate early risk assessment.

Page 29 of 107 | Total Record : 1068

 27   28   29   30   31 

Filter by Year

2009 2023


Reset
Filter By Issues
All Issue Vol 15, No 3 (2023): December 2023 Vol 15, No 2 (2023): August 2023 Vol 15, No 1 (2023): April 2023 Vol 14, No 3 (2022): Desember 2022 Vol 14, No 2 (2022): August 2022 Vol 14, No 1 (2022): April 2022 Vol 13, No 3 (2021): December 2021 Vol 13, No 2 (2021): August 2021 Vol 13, No 1 (2021): April 2021 Vol 12, No 3 (2020): December 2020 Vol 12, No 2 (2020): August 2020 Vol 12, No 1 (2020): April 2020 Vol 12, No 1 (2020): April 2020 Article-in-Press Vol 11, No 3 (2019): December 2019 Vol 11, No 2 (2019): August 2019 Vol 11, No 1 (2019): April 2019 Vol 11, No 1 (2019): April 2019 Vol 11, No 3 (2019): Article-in-Press Vol 10, No 3 (2018): December 2018 Vol 10, No 3 (2018): December 2018 Vol 10, No 2 (2018): August 2018 Vol 10, No 2 (2018): August 2018 Vol 10, No 1 (2018): April 2018 Vol 10, No 1 (2018): April 2018 Vol 9, No 3 (2017): December 2017 Vol 9, No 3 (2017): December 2017 Vol 9, No 2 (2017): August 2017 Vol 9, No 2 (2017): August 2017 Vol 9, No 1 (2017): April 2017 Vol 9, No 1 (2017): April 2017 Vol 8, No 3 (2016): December 2016 Vol 8, No 3 (2016): December 2016 Vol 8, No 2 (2016): September 2016 Vol 8, No 2 (2016): September 2016 Vol 8, No 1 (2016): March 2016 Vol 8, No 1 (2016): March 2016 Vol 7, No 2 (2015): September 2015 Vol 7, No 2 (2015): September 2015 Vol 7, No 1 (2015): March 2015 Vol 7, No 1 (2015): March 2015 Vol 6, No 2 (2014): September 2014 Vol 6, No 2 (2014): September 2014 Vol 6, No 1 (2014): March 2014 Vol 6, No 1 (2014): March 2014 Vol 5, No 2 (2013): September 2013 Vol 5, No 2 (2013): September 2013 Vol 5, No 1 (2013): March 2013 Vol 5, No 1 (2013): March 2013 Vol 4, No 2 (2012): September 2012 Vol 4, No 2 (2012): September 2012 Vol 4, No 1 (2012): March 2012 Vol 4, No 1 (2012): March 2012 Vol 2, No 2 (2010): September 2010 Vol 2, No 2 (2010): September 2010 Vol 2, No 1 (2010): March 2010 Vol 2, No 1 (2010): March 2010 Vol 1, No 1 (2009): March 2009 Vol 1, No 1 (2009): March 2009 More Issue