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Biosaintifika: Journal of Biology & Biology Education

biosaintifika
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Published by Universitas Negeri Semarang

ISSN : 2085191X EISSN : 23387610 DOI : -

Core Subject : Agriculture,

Agriculture, Biological Sciences & Forestry

Biosaintifika Journal of Biology & Biology Education, an electronic international journal, provides a forum for publishing the original research articles, review articles from contributors, and the novel technology news related to biological research and biological education.

Arjuna Subject : -

Articles 1,068 Documents

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Seed Exploration for Seed Banking Purpose in Cibodas Botanical Garden Zuhri, Musyarofah; Latifah, Dian; Kurniawati, Fitri; Noviady, Ikhsan; Suhendri, Yudi
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 1 (2019): April 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Due to contribution of Target 8 of the Global Strategy for Plant Conservation GSPC Botanic Gardens, Cibodas Seed Bank committed to increase its ex situ plant collection through seed banking. This research aimed to assess population and collect seeds from Cibodas remnant forests and its neighboring, Mt. Gede Pangrango National Park forest, for seed banking purpose. Thirty-two numbers of seeds belonging to fifteen families were collected. Two species were included in IUCN red list i.e. Saurauia cauliflora (vulnerable) and Magnolia blumei (least concern) and one species classified as endangered species by World Conservation Monitoring Unit (WCMC) i.e. Pinanga javana. Sixty-five percent population were possible to collect without affecting the availability of the seeds in the nature. Around a half of seeds were collected at natural dispersal stage to ensure the seed maturity. Ninety-one percent collected seeds were expected as orthodox seeds that can be stored in Cibodas Seed Bank and three species were recalcitrant and cannot be stored in Cibodas Seed Bank i.e. Calamus ciliaris, C. reinwardtii and Daemonorops rubra. Cut-test results showed 78% seed collected were full seeds, that were not infested, empty or immature seeds. This study provides information regarding the readiness of population for seed collection and species were collected and stored in Cibodas Seed Bank.

DNA Barcoding: A Study of Guppy Fish (Poecilia reticulata) in East Java, Indonesia Hariyanto, Sucipto; Adro’i, Hasan; Ali, Mahrus; Irawan, Bambang
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 2 (2019): August 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Poecilia reticulata is a freshwater fish from the northeastern part of South America and spread widely to various countries in Asia and other continents. However, research about P. reticulate is limited even though it is a well-known fish species in Indonesia. The purpose of study was to identify the fish species of P. reticulata through DNA barcoding using the COI gene to determine the phylogenetic relationships among fish populations in East Java, Indonesia. In a present study, there were eight samples of P. reticulata from four different freshwater locations in East Java. Extraction, amplification, and sequencing of DNA samples were conducted to obtain the genetic data and construct a phylogenetic tree based on DNA sequences. The COI gene is the most popular markers to study genetic populations and phylogeography among the animal kingdom. Our phylogenetic reconstruction showed a clear that there were two groups of P. reticulata. The first group was obtain through species from East Java, Sukabumi, West Java (KU692776.1), Dominican Republic, Pandeglang, Banten and Myanmar. The second group was P. reticulata from southern Africa, Brazil, and Sukabumi, West Java (KU692775.1). The result of this study indicate that the guppy fish in East Java identic with P. reticulata from West Java (KU692776.1), which a widely used in classification based on evolutionary relationships. The findings of this study have important implication for the development of advance research about adaptation, phylogeny, and evolution of fish, especially of guppy fish.

Antioxidant and Antiglycation Activity of Rhizosphere and Endophytic Actinobacteria of Xylocarpus granatum Ariansyah, Alifian; Batubara, Irmanida; Lestari, Yulin; Egra, Saat
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 2 (2019): August 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Utilization of endophytic bacteria such as actinobacteria is one of the alternatives to obtain the bioactive compounds similar to the host plant. This study aimed to identify compounds produced by rhizosphere and endophytic actinobacteria isolated from Xylocarpus granatum for their antioxidant and antiglycation activity. Actinobacterial culture supernatant was extracted using ethyl acetate with a ratio of 1:1 (v/v). Antioxidant activity was performed using 2,2-diphenyl-1-picrylhydrazyl (DPPH). Whole TLC-bioautography and phytochemical tests were used to predict the group of active compounds. Antiglycation activity was identified based on the ability to inhibit the formation of advanced glycation end products (AGEs). Fifteen actinobacterial isolates had been isolated from rhizosphere (ten isolates), fruit (two isolates), and leaf (two isolates) of X. granatum. All isolates had been tested for their antioxidant and antiglycation activity. Isolate XR2 had the highest antioxidant activity with IC50 value of 1719.26 mg/L. Meanwhile, the highest antiglycation activity was obtained from isolate XR8 with IC50 value of 327.62 mg/L. This study informs that actinobacteria also live in the fruit, leaves and rhizosphere of X. granatum. The existence of rhizosphere and endophytic actinobacteria from X. granatum and their antioxidant and antiglycation activities contributes to the understanding of their diversity and potency as an antioxidant and antiglycation agent.

The Effect of Turmeric and Ginger Powder on Japanese Quail (Coturnix coturnix) Serum Lipid Profile Sulastri, Meilinda Pahriana; Basri, Hasan
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 2 (2019): August 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Lipids are essential fatty acids. Lipids function as a source of energy and structural constituent components of Japanese quails. Japanese quail (Coturnix coturnix japonica) is one of the livestock sectors that is efficient in providing nutrients, both from eggs and meat. However, this animal has high cholesterol levels. This study aimed to determine the effect of organic feed on the cholesterol, triglyceride, HDL (High Density Lipoprotein), and LDL (Low Density Lipoprotein) level of Japanese quail. This study used an experimental method with a completely randomized design (CRD). The test animals were 30 females Japanese quail which were divided into 3 groups (2 individuals each) with different treatments i.e. P0: control (commercial feed); P1: standard organic feed (corn, soybean, bran and fish meal); P2: special organic feed (corn, soybean, bran, fish meal, cassava leaves, turmeric and ginger powder). Each treatment consisted of 5 replications. Feed was given for 2 months and at the end of the treatment, the blood was collected for analysis. Data obtained were analyzed using analysis of variance (ANOVA) and continued with Duncan test with a confidence of 95% (α=0.05). Analysis was performed using SPSS software 16.0 for windows. The results showed that the effect of organic feed containing cassava leaves, turmeric and ginger powder is significantly different on live weight, and not significantly different on feed consumption and drinking consumption. Moreover, the provision of that special organic feed was able to stabilize the cholesterol and HDL levels, and reduce LDL levels in Japanese quail (Coturnix coturnix japonica).

Biosorption of Heavy Metal Pollution by Enterobacter agglomerans Dewi, Nur Kusuma; Mubarok, Ibnul; Yuniastuti, Ari
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 2 (2019): August 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v11i2.20471

Biosorption is a new waste treatment technology that can eliminate toxic heavy metals. Biosorption can be considered as an environmentally friendly alternative technology to treat industrial liquid waste that is economically proper to use. One of them is biosorption that utilizes the microorganisms’ absorption ability, especially bacteria that can absorb heavy metals in waters, such as Enterobacter agglomerans. This research aimed to determine the ability of E. agglomerans in reducing heavy metals pollution in local river. The research employed the measurement of the effect of lead (Pb) to E. agglomerans growth using Optical Density (OD) at wavelength 600 nm. The colony numbers were calculated using a standard curve. While the ability of E. agglomerans to reduce heavy metals concentration in liquid media was measured using AAS with a wavelength of 240 nm. The results showed that lead affected the growth of E. agglomerans. The OD value has a negative relationship with the concentration level of Pb. The ODs were decreased from 2.867 to 1.242, using Pb level from 0 ppm to 20 ppm. Therefore, it proved that E. agglomerans could reduce heavy metals concentration in local river in Central Java Province. This research was the first report on E. agglomerans activity on heavy metal in contaminated water. This result can be used as a reference for industrial sites near the river to treat their wastewater before discharging it to the river body to preserve its water purity.

The Species Diversity and Structure of The Limited Production Forest in Arui Watershed of Manokwari District of West Papua, Indonesia Mahmud, Mahmud; Kusumandari, Ambar; Sudarmadji, Sudarmadji; Supriyatno, Nunuk
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 2 (2019): August 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v11i2.16340

The detailed causal factors of flood have not been established, whether because of the damage of upper watershed, sedimentation resulting in shallow river, forest conversion, the decrease in abundance and structure or high rainfall. The study aimed at finding out the species diversity and structure of the limited production forest (LPF) from the seedling to the tree stage of Arui watershed in Manokwari district. The species diversity was determined based on Shannon-Wiener index, while vegetation structure was determined based on Importance Value Index. There were totally 92 plots of samples drawn using systematic sampling. The results of vegetation analysis showed that there were 174 species of 43 families with the species diversity of 1.5-1.8 that was categorized as moderate. The four dominant species that were always found in seedling, sapling, pole and tree stages included: Pometia pinnata, Teijsmanniodendron bogoriense, Chisocheton ceramicus, and Horsfieldia irya. The conversion of the LPF into non-forestry sector for the purpose of accelerating development must be reviewed by considering hydrology, land, biodiversity, and social and regional aspects in order to prevent and to reduce flood in the coming days.

Histological Study of White Rats Kidney Following The Consumption of Obat Pahit From Riau Archipelago Fitmawati, Fitmawati; Saputra, Agus; Titrawani, Titrawani; Juliantari, Erwina; Kusumo, Awal Prichatin
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 2 (2019): August 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Obat Pahit from Lingga Malay ethnic is a traditional medicine which has believed by the local people to maintain the body stamina (immunomodulators and antioxidants). This study aimed to investigate the effect of Obat Pahit Potion on kidney histological structure of white male rats (Rattus norvegicus). This research was an experimental study with a Randomized Complete Block Design consisted of 15 treatments and 3 replications. The experiment groups consisted of control groups (Stimuno, distilled water, and CMC-Na 1%) and three treatment groups of Obat Pahit Potion (Kalan, SP4, Linau) with 4 different dosages. The samples of a kidney of white rats were prepared for histological observation using paraffin method and Hematoxylin-Eosin staining. The results of this study showed that three kinds of Obat Pahit with four different dosages showed the expansion of glomerular cells on kidney tissue. Damage that occurs in the kidneys is still in small amounts and normal range which is less than 25%. The results of this study provide information for the society that the consumption of Obat Pahit Potion will not cause toxicity effect on the kidney.

Ethnobotanical Study on Medicinal Plants in Sesaot Forest, Narmada,West Lombok, Indonesia Rahayu, Slamet Mardiyanto; Andini, Arista Suci
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 2 (2019): August 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Indonesia is one of the largest mega biodiversity countries in the world that is rich in biological resources. Plants can be an alternative in treating diseases by Indonesian people such as around the Sesaot Forest, the Buwun Sejati Village. This study aimed to determine the types of plants in Sesaot Forest which were used as medicine by the people of Buwun Sejati Village, Narmada District, West Lombok Regency. This research was conducted by interview method and field survey. Data analysis was carried out descriptively. Based on the research, there were 87 species included in 42 families used as medicine by the community of Buwun Sejati Village. The Zingiberaceae family was a plant family that was most widely used as a traditional medicine ingredient. There were 30 types of diseases treated using Sesaot Forest plants by the community of Buwun Sejati Village. Leaves were of the most common part of plant used as raw material for traditional medicine by the people of Buwun Sejati Village, Narmada District, West Lombok Regency. The advantages of traditional medicines include its widespread accessibility and relative low prices. Therefore, this data obtained from this study is necessary to inventory the kind of medicinal plants and their utilization by the community, so that, the traditional knowledge of the medicinal plants can be documented and preserved.

The Potential Endophytic Bacteria Isolated from Rice (Oryza sativa) as Biofertilizer Putriani, Putriani; Fitri, Lenni; Ismail, Yulia Sari
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 2 (2019): August 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Endophytic bacteria are beneficial microorganisms that interact with host plants without causing any interference or damage to plants. This research aimed to obtain endophytic bacteria isolated from the root tissue of rice plants (Oryza sativa L.) which have potential to produce IAA hormones,, identify the endophytic bacteria in morphologically and physiologically, and analysis of the selected isolate 16S rRNA genes. Based on the results of this research, there were six endophytic bacteria isolates obtained. They have high morphological diversity and differen ability producing IAA hormones. The highest concentration of IAA (425 ppm) was obtained isolates from EAP3. Isolate EAP3 also produce inhibit the growth of Xanthomonas oryzae with a 5.2 mm inhibition zone. Based on the biochemical test, EAP3 had 60% similarity with Enterobacter asburiae. Analysis of the 16S rRNA gene showed that EAP3 had the highest similarity with Enterobacter asburiae strain U4 by 99%. This research data is considered as new information about the potential of endophytic bacteria from the roots of rice plants (Oryza sativa L.) which is capable of producing IAA hormones and is able to inhibit pathogenic bacteria. This research provides information that can be used as a basis for developing endophytic bacteria as biological fertilizers.

Screening of Bacteria Producing Asparaginase Free of Glutaminase and Urease from Hot Springs in West Sulawesi Setiawan, Ruby; Larasati, Dinar Rahmi
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 2 (2019): August 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

L-asparaginase catalyzes the hydrolysis of asparagine into ammonia and aspartate. It has been used in chemotherapy for patients with acute lymphoblastic leukemia. L-asparaginase presents in animal, plant and microorganism. Long-term application of this enzyme can induce neurotoxicity due to the affinity towards glutamine and urea. The aim of this research was to find new source of glutaminase and urease-free asparaginase from bacteria. Bacteria were isolated from hot springs located in West Sulawesi using R2A media. The identification was employed by amplifying 16S rRNA gene. Screening of asparaginase was conducted using asparagine as single source of Nitrogen. Out of 21 isolates, 76% were Gram-negatives from the genus of Pseudomonas, Acinetobacter, Bosea, Caulobacter, Sphingomonas and Novosphingobium, while the rest of them were Gram-positives from the genus of Mycobacterium, Brachybacterium, Rhodococcus, and Staphylococcus. Twelve isolates which showed asparaginase activity were Caulobacter flavus HS1YWS2 and HS1XWS3, Acinetobacter sp. HS2XWS5, HS2XWS6, HS2XWS8, HS2YWS11, HS2YWS12, HS2YWS13, HS2ZWS14, HS2ZWS15 and HS2ZWS16. Isolates HS1YWS2 and HS1XWS3 were free of glutaminase and urease and showed the highest activity. This study was the first report of asparaginase activity from Caulobacter flavus. This result can further be used to explore the ability of asparaginase free of glutaminase and urease to treat acute lymphoblastic leukemia.

Page 35 of 107 | Total Record : 1068

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Contact Name
Dr. Ir. Dyah Rini Indriyanti

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biosaintifika@mail.unnes.ac.id

Phone
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Journal Mail Official
biosaintifika@mail.unnes.ac.id

Editorial Address
-

Location
Kota semarang,

Jawa tengah

INDONESIA