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Biosaintifika: Journal of Biology & Biology Education
Published by Universitas Negeri Semarang
ISSN : 2085191X     EISSN : 23387610     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Biosaintifika Journal of Biology & Biology Education, an electronic international journal, provides a forum for publishing the original research articles, review articles from contributors, and the novel technology news related to biological research and biological education.
Arjuna Subject : -
Articles 1,068 Documents
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Antidiabetic Potentials of Button Mushroom (Agaricus bisporus) on Alloxan-Induced Diabetic Rats Nuraeni Ekowati; Nilasari Indah Yuniati; Hernayanti Hernayanti; Nuniek Ina Ratnaningtyas
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 3 (2018): December 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i3.17126

Abstract

Button mushrooms (Agaricus bisporus) is an edible mushroom that is most widely cultivated in the world. It contains bioactive compounds that might provide beneficial effects on diabetes mellitus patient. The study aimed to determine the effects of A. bisporus administration on the blood glucose, and malondyaldehyd (MDA) levels as well as superoxide dismutase (SOD) activity of alloxan-induced diabetic rats. This study was also conducted to determine the secondary metabolites produced by A. bisporus. The method used was experimental methods with Completely Randomized Design. A. bisporus extract at the doses of 250, 500 and 750 mg/kg body weight (BW) per day were orally applied to alloxan-induced diabetic rats for a period of 14 days after the rats became diabetes. The results showed that the extract of A. bisporus could decrease blood glucose, and MDA levels as well as increase SOD activity (p 0.05). A. bisporus extract 500 mg/kg BW is the most effective dose to be used. Based on Thin Layer Chromatography (TLC) test, it was known that secondary metabolites produced by A. bisporus are flavonoids, alkaloids, terpenoids and saponins. A. bisporus has potential as an antidiabetic through the ability to decrease blood glucose, and MDA levels, as well as increase SOD activity in diabetic rats. This research is able to provide information about the antidiabetic potential of A. bisporus extract so that it can be used as an alternative natural antidiabetic agent and can be applied in the community with ease and in a more controlled industrial scale.
Development of Life Skill-Based Learning Instruments: Biotechnology Material in Junior High School Sekar Jatiningrum Parnonansia; Dewi Mustikaningtyas
Biosaintifika: Journal of Biology & Biology Education Vol 7, No 1 (2015): March 2015
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v7i1.3537

Abstract

The result of preliminary observations in Empu Tantular Semarang junior high school showed that the learning instruments was still focused on the achievement of cognitive competence. The present study aimed to develop a life skill-based learning instruments of biotechnologi material. Learning instruments including syllabi, lesson plans, worksheets, scale attitudes sheets, and vocational skills assessment sheet, as well as to test its feasibility and effectiveness. This study was Research and Development, whereas the trial product design was a One Shot Case Study pattern. Trials of limited scale and large scale were done in the student of class IX at odd semester of year 2014/2015. The results of the three expert assessment of learning instruments showed very feasible criteria to be used. Inter-rater correlation analyzes were performed using the SPSS16 program which obtained a yield of 0.99. It indicated that the inter-rater reliability was exceptionally high. Classical completeness to the cognitive learning, affective and vocational skills of students of class IX A were 92.1%; 97.4%; 92.1%, respectively. Meanwhile, the results of class IX D were 74.4%; 94.9%; 100%, respectively. The percentage of student who reach the level of adherence to the learning ≥61% were 100% in class IX A and 87.2% in class IX D. Therefore, the developed learning instrument was fit to be used and also effectively applied in the Empu Tantular Semarang junior high school. Beside of improving student cognitive competence in Empu Tantular Semarang junior high school,  development of life skill-based learning instruments can also provide knowledge and skills that can be used so that students can make a positive contribution in later life as a member of the family and society. Hasil Observasi awal di SMP Empu Tantular Semarang, menunjukkan perangkat pembelajaran yang diterapkan masih terfokus pada pencapaian kompetensi kognitif. Penelitian ini bertujuan untuk mengembangkan perangkat pembelajaran IPA berbasis kecakapan hidup materi bioteknologi yang meliputi silabus, RPP, LKS, dan instrumen penilaian serta menguji kelayakan dan efektivitasnya. Metode penelitian yang digunakan adalah Research and Development (RD). Desain uji coba produk menggunakan pola One Shot Case Study. Uji coba skala terbatas dan skala luas dilakukan pada peserta didik kelas IX semester gasal tahun ajaran 2014/2015. Hasil penilaian dari ketiga pakar terhadap silabus, RPP, LKS, lembar skala sikap, dan lembar penilaian kecakapan vokasional mencapai kriteria Very Feasible digunakan. Analisis korelasi inter rater yang dilakukan menggunakan program SPSS16 memperoleh hasil sebesar 0,99. Hal ini menunjukkan bahwa reliabilitas inter rater/antar penilai tergolong sangat tinggi. Ketuntasan klasikal untuk hasil belajar kognitif, afektif dan kecakapan vokasional dari kelas IX A berturut turut 92,1%; 97,4%; 92,1% dan kelas IX D berturut turut 74,4%; 94,9%; 100%. Persentase jumlah anak yang mencapai tingkat keterlaksanaan pembelajaran ≥ 61% dari kelas IX A sebesar 100% dan kelas IX D sebesar 87,2%.Perangkat pembelajaran yang dikembangkan Very Feasible digunakan dan efektif diterapkan di SMP Empu Tantular Semarang.
High Connectivity Among Synedrella nodiflora Populations in Java Island Based on Intergenic Spacer atpB-rbcL Agus Hery Susanto; Agus Nuryanto; Budi Setiadi Daryono
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 1 (2018): April 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i1.12038

Abstract

Synedrella nodiflora (L.) Gaertn has taxonomically been the only species of genus Synedrella, which spreads over many tropical countries. In spite of its wide range of distribution, the genus remains monospecific. This leads to assumption of the very low genetic diversity among S. nodiflora populations worldwide. It may also be the case in Java Island, though rapid changes in ecosystem condition occurs. Here we report our study on S. nodiflora population genetics in Java Island using intergenic spacer (IGS) atpB –  rbcL as a molecular marker, since it has been well known as one of the most variable chloroplast genome regions in a wide range of plant species so far. As many as 58 individuals were collected randomly from ten different locations in the island. Based on IGS atpB – rbcL sequences of 860 bp length, only two haplotypes were observed. Both show only one polymorphic site (0.12%) and one transversion, where T is substituted by G at position 790, indicating that high connectivity among populations of S. nodiflora in Java Island is observed. This results in a low genetic differences among the populations, which at the same time provides a fact of nearly no variation among the IGS atpB – rbcL sequences.
The Diversity of Arthropods Predator on Wild Plant of Rice Field with and without Pesticides Dewi Meidalima; Ruarita Ramadhalina Kawaty; Erlan B. Gunawan
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 1 (2017): April 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i1.5124

Abstract

Contaminated- agro ecosystem influences abundance and diversity of arthropods. The aims of this study  were to analyze the diversity and abundance of pre-planting  arthropod predators  on wild plants in rice field with and without  application of pesticides. The survey and direct observation of wild plants at the rice filed were conducted from January to March 2015, at 1 ha in Pemulutan and  1 ha in Musi 2 Palembang. Sampling of arthropod predator was conducted 8 times before the rice was grown, using insect nets. On the land without pesticide application was found 14  arthropod families which consists of 28 species and 15 families of wild plant consists of 25 species.  On the land with pesticide application  was found  8 arthropod families consists of 16 species and 15 wild plant families with 23 species. On the land without pesticide application was found High index of diversity (H’=3.121)  and low dominance (D=0.095), while on the land with pesticide application low index of diversity (H’=2.602) and high dominance (D=0.171). It is the indicators of arthropods predator more varieties at the land without pesticide application compare to the land with pesticide application. This finding is very important for biological pest management in South Sumatra. 
Resistance Monitoring of Nilaparvata lugens Stall against Pymetrozine Insecticide with Determination of Diagnostic Concentrations Sri Murtiati; Udi Tarwotjo; Rully Rahadian
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 1 (2021): April 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i1.28237

Abstract

Nilaparvata lugens Stall is one of the main insect pests on rice crops. Intensive control of this pests using insecticides has resulted in the development of insect resistance. This study aimed to find out the level of resistance of the N. lugens population to pymetrozine insecticides by determining the diagnostic concertations. N. lugens was collected from five endemic areas in Central Java Province from October 2019 to June 2020. The data from the bioassay test were analyzed with probit analysis to obtain the LC50 value. The results of the sensitivity test showed that the Kajen population has the highest RF value (2.47), while the Karanganyar population which has the lowest RF value (1) was the most sensitive population. The determined diagnostic concentration was LC95 = 25.52 ppm with the lowest concentration limit of 7.67 ppm and the highest of 30.05 ppm. To conclude, the determined diagnostic concentration (LC95 = 25.52 ppm) is effective for detecting the susceptibililty of N. lugens population. This finding would be beneficial for monitoring resistance of N. lugens population against pymetrozine insecticides in the field.
Application of Bio P60 and Bio T10 in Combination Against Phytophthora Wilt of Papaya Loekas Soesanto; Kustam Kustam; Endang Mugiastuti
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 3 (2019): December 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v11i3.20389

Abstract

Papaya is one of the most widely cultivated horticultural plants. Phytophthora wilt is an important papaya disease which results in production losses. This research aimed to determine the effect of Bio P60 (raw secondary metabolites of Pseudomonas fluorescens P60) and Bio T10 (raw secondary metabolites of Trichoderma harzianum T10) application in combination on Phytophthora wilt and on growth of pepaya. The research was conducted on Californian pepaya farm, Linggasari Village, Kembaran District, Banyumas Regency. Randomized block design was used with six replicates and five treatments consisted of control (Mancozeb 80%), Bio T10 flush + Bio T10 spray, Bio T10 flush + Bio P60 spray, Bio P60 flush + Bio T10 spray, and Bio P60 flush + Bio P60 spray. Variables observed were percentage of healthy leaves, infection rate, number of healthy leaves, and number of healthy pepaya fruit. Result of the research showed that application of Bio P60 and Bio T10 effectively cured Phytophthora wilt with the percentage of healthy leaves as 69.19% compared to control. The combination was able to increase the number of healthy leaves the number of healthy papaya fruit. The novelty of this research is that raw secondary metabolites of biological agents proven to be able to overcome the papaya diseases, which so far cannot be solved. The implication for the development of science is one step ahead in overcoming plant diseases biologically by utilizing raw secondary metabolites. The benefits for the community can overcome papaya plant diseases organically, safely, and environmentally friendly, and inexpensive.
Neurogenic Differentiation of Bone Marrow Mesenchymal-Like Stem Cell Induced by Delonix regia Flowers Extract Kartini Eriani; Irma Suryani; Al Azhar; Risa Nursanti; Ichsan Ichsan; Arief Boediono
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 2 (2018): August 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i2.15051

Abstract

Stem cell technology has great potential in the effort to cure degenerative diseases. This study was done to determine optimum dose of flamboyant (Delonix regia) flower extract to induce proliferation and differentiation of mice (Mus musculus) bone marrow mesenchymal-like stem cell. Bone marrow cells were collected from mice by aspiration. Cells suspension (1 x 106) were poured into petri dishes containing 2 ml of modified Dulbecco's Modified Eagle's Media (mDMEM) and incubated overnight at 37 °C in a 5% CO2 incubator and microscopically observed. In quadriplicate, MSC were cultured in mDMEM containing D. regia flower extract of 0.0 (control), 0.4, 0.6, 0.8, and 1.0 mg/ml and incubated at 37 °C for 9 days. Population doubling time (PDT) and differentiated cell type were microscopically observed using HE staining on day 1 and 10. Data obtained were analyzed by ANOVA and Tukey test. The results showed that the addition of D regia flowers extracts 0.8 and 1.0 mg/ml significantly reduced PDT compared to that of 0.4, 0.6 and control. The extract, at 0.4 and 0.6 mg/ml, were able to induce MSC differentiation into fibroblast-like and nerve-like cells. In conclusion, D. regia flower extracts of 0.6, 0.8 and 1.0 mg/ml were able to stimulate MSC proliferation, but optimum dose for neurogenic differentiation was 0.6 mg/ml. This is the first to show potential of D. regia flower extract as neurogenic differentiatian inducer on mice MSC. These findings can be used as preliminary information for using the extract as cellular differentian inducer in basic and applicative reseach using stem cells.
Rambut Sebagai Bioindikator Pencemaran Timbal Pada Penduduk di Kecamatan Semarang Utara Aditya Marianti; Agung Tri Prasetya
Biosaintifika: Journal of Biology & Biology Education Vol 5, No 1 (2013): March 2013
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v5i1.2568

Abstract

Timbal (Pb) bersifat toksik, karsinogenik, bioakumulator dan biomagnifikasi. Bioakumulasi Pb dapat terjadi pada kuku, hati dan rambut. Penelitian bertujuan untuk mendeteksi apakah telah terjadi pencemaran timbal pada penduduk di kecamatan Semarang Utara dengan menggunakan rambut sebagai bioindikator. Penelitian deskriptif eksploratif ini menggunakan populasi seluruh penduduk di kecamatan Semarang Utara. Sampel diambil dengan teknik purposive sampling yaitu penduduk laki-laki berumur antara 17 sampai dengan 50 tahun yang tinggal di kelurahan Tanjung Mas dan Bandarharjo Semarang Utara, minimal selama 5 tahun terakhir terus menerus. Kadar timbal pada tubuh dideteksi dari kadar timbal yang terakumulasi pada rambut. Hasil penelitian menunjukkan terdapat kandungan timbal pada rambut 56 sampel. Kadar timbal tertinggi sebesar 17,028 ppm dan rata-rata 8,304 ppm. Penyebabnya diduga bukan berasal dari emisis kendaraan bermotor di jalan raya mengingat profesi sampel sebagian besar adalah nelayan, tetapi kemungkinan berasal dari air minum yang mereka konsumsi. Sampel air minum yang dikonsumsi terdeteksi mengandung timbal rata-rata 6 ppm. Simpulan dari penelitian ini telah terjadi pencemaran timbal pada sebagian penduduk Semarang Utara dengan tingkat ringan sampai sedang. Pencemaran diduga berasal dari air minum yang dikonsumsi.Lead (Pb) are toxic, carcinogenic, bioaccumulator and biomagnification. Pb bioaccumulation may occur in the nail, liver and hair. The research aimed to detect whether lead pollution has affected the people in North Semarang, using hair as bioindicator. The population of this explorative descriptive research was all residents of Sub-District of North Semarang. Samples were taken using purposive sampling technique, i.e. male residents of Tanjung Mas and Bandarharjo aged between 17 and 50 years, who have stayed in the sites for at least 5 years without interruption. The lead level in their body was detected from their hair strand using AAS (Atomic Absorption Spectrophotometery). Research showed that lead substance was detected in hair strands from 56 people. The highest level was 17.028 ppm and the average level was 8,304 ppm. It was suggested that the lead pollutant was not from motor vehicles emisions on the roads cause most samples were fishermen, instead it was suspected that the drinking water was the source of the lead. It was supported by the fact that the drinking water consumed daily by the residents has been contaminated by lead at the level of 6 ppm. Conclusion the lead pollution has affected some residents of North Semarang, ranging from low level to medium level. Presumably, the pollution was originated from the consumed drinking water.
Effect of Carica papaya Leaf Juice on Hematology of Mice (Mus musculus) with Anemia Afreni Hamidah; Evita Anggereini; Nurjanah Nurjanah
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.11427

Abstract

Anemia is defined as a decrease in haemoglobin concentration and the number of erythrocytes. One source of iron in vegetable is the papaya leaf. This study aimed to determine the number of erythrocytes and hemoglobin levels after the application of Carica papaya leaf on mice through the sodium nitrite induction. The research design used was Randomized Design Complete by using five treatments with five repetitions on each treatment. The data measured in this study was number of erythrocytes and hemoglobin levels of rats before being given sodium nitrite, after being given sodium nitrite and after being given papaya leaf juice. The data obtained were analyzed statistically by using variance analysis (ANOVA), and the data that significantly different was then analyzed by Duncan Multiple Range Test (DMRT). The results showed that papaya leaf juice significantly affected the increase of erythrocyte number and hemoglobin level. The concentration that gives the best effect in increasing the number of erythrocytes is 50% papaya leaf juice concentration and 25% papaya leaf juice concentration which gives the best effect in increasing the hemoglobin content. Further, the findings can be used as baseline information for further scientific investigation for using papaya leaf as an alternative medicine in curing anemia disease and analyzing phytochemical, pharmaceutical and other biological activities on other plant or vegetable and to conducted further research by using female mice and pregnant female mice.
The Effectiveness of Experimental Diet with Varying Levels of Papain on The Growth Performance, Survival Rate and Feed Utilization of Keureling Fish (Tor tambra) Zainal Abidin Muchlisin; Fardin Afrido; Tanzil Murda; Nur Fadli; Abdullah A. Muhammadar; Zulkarnain Jalil; Cut Yulvizar
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 2 (2016): September 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i2.5777

Abstract

The objective of present study was to determine the optimum level of papain in the diet of keureling fish (Tor tambra). The complete random design was utilized in this study. Six levels of papain dosage were tested in triplicates, i.e. 0 (control); 17.5 mg kg-1,  20.0 mg kg-1, 22.5 mg kg-1, 25.0 mg kg-1 and 27.5 mg kg-1 of feed. The experimental fish were fed the experimental diet two times a day at 8 AM and 5 PM at feeding level of 5% body weight for 90 days. The Anova test result showed that papain enzyme  gave a significant effect on the weight gain, daily growth rate, specific growth rate, survival rate, feed conversion ratio and feed efficiency (P0.05). The Duncan multi-rage test result showed that the higher values for all measured parameters were obtained at the dosage of 27.5 mg kg-1. Therefore, it is concluded that the optimum dosage of papain enzyme for keureling fish was 27.5 mg kg-1 of feed.How to CiteMuchlisin, Z. A., Afrido, F., Murda, T., Fadli, N., Muhammadar, A. A., Jalil, Z., Yulvizar, C. (2016). The Effectiveness of Experimental Diet with Varying Levels of Papain on The Growth Performance, Survival Rate and Feed Utilization of Keureling Fish (Tor tambra). Biosaintifika: Journal of Biology Biology Education, 8(2), 172-177.

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