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Biosaintifika: Journal of Biology & Biology Education
Published by Universitas Negeri Semarang
ISSN : 2085191X     EISSN : 23387610     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Biosaintifika Journal of Biology & Biology Education, an electronic international journal, provides a forum for publishing the original research articles, review articles from contributors, and the novel technology news related to biological research and biological education.
Arjuna Subject : -
Articles 1,068 Documents
 85   86   87   88   89 
Induction and Growth Kinetics Callus of Tomato (Solanum lycopersicum) Arkan Setiaji; RR Rifka Annisa; Rumiyati Rumiyati; Endang Semiarti
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i1.21704

Abstract

Plant callus extracts are potential to be developed as ingredient in skincare products. Tomato callus is supposed to contain protein-derivatives and or other components such as secondary metabolites that play a role in skin regeneration. Therefore the production of calli is important to be studied for callus sustainable supply. This research aims to obtain optimum medium for callus induction and to analyze tomato callus development anatomically. In vitro culture response was assessed in tomato plant (Solanum lycopersicum L. ‘Permata’) for optimum callus induction. Seeds were grown on ¼ MS medium for 10-15 days. Hypocotyl was excised and cultured on MS medium + 2 mg/l 2,4-D for 15 days as the explants for callus induction. Callus was transferred to MS medium with 8 variations of PGRs including the combination of BAP + NAA, and 2,4-D. Both fresh and dry weight was measured every 5 days over 60 days to establish the growth kinetics and growth efficiency of callus. Anatomic characters of calli were examined through paraffin-embedded method. The result showed of MS medium supplemented with 2.0 mg/l NAA and 0.2 mg/l BAP is optimum for tomato callus induction, based on highest number of the absolute growth rate on fresh weight (73.77% per day), dry weight (3.84% per day), and callus initiation time (5.56 days) achieved by the medium. Cells in the ground tissue of tomato hypocotyl are competent to be dedifferentiated into a callus. This research results were expected to find out suitable methods for tomato callus production in preparation for skincare uses.
Profile of SGPT and SGOT on Male Rats (Rattus norvegicus) Hyperglycemic After Giving Insulin Leaf Extract (Tithonia diversifolia) Rizki Fitrawan Yuneldi; Tyas Rini Saraswati; Enny Yusuf Wachidah Yuniwarti
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 3 (2018): December 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i3.5516

Abstract

The leaves of insulin (Tithonia diversifolia (Hemsl.) A. Gray) are native bush plants from Central America. This plant in Indonesia is often called paitan or kembang bulan. The aim of this research was to know the profile of SGPT and SGOT of male white rat (Rattus norvegicus L.) hyperglycemic after giving insulin leaf extract (Tithonia diversifolia (Hemsl.) A. Gray). This study used 20 male Wistar rats which were divided randomly into 5 groups of treatment. Those were P0 as a group of rats which was not given alloxan and insulin leaf extract, P1 as a group of rats which was given alloxan without insulin leaf extract, P2 as a group of rats which was given alloxan and glibenclamide 2 mg/Body Weight (BW)/day, P3 as a group of rats which was given alloxan and insulin leaf extract 30 mg/BW/day, P4 as a group of rats which was given alloxan and insulin leaf extract 60 mg/BW/day. Each treatment was repeated as many as 4 replications. The results of this study indicated that there was no significant differences in all variables, namely levels of SGPT, SGOT, liver weight, body weight and Hepatosomatic index (HSI), so it can be concluded that giving insulin leaf extract (Tithonia diversifolia (Hemsl.) A. Gray) of 30 mg/BW/day and 60 mg/BW/day could improve liver function of hyperglycemic male white rats (R. norvegicus L.).
KONSERVASI BERUANG MADU DI KWPLH BALIKPAPAN Sri Ngabekti
Biosaintifika: Journal of Biology & Biology Education Vol 5, No 2 (2013): September 2013
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v5i2.2751

Abstract

AbstrakBeruang madu (Helarctos malayanus) merupakan salah satu fauna  yang dilindungi  perundang-undangan Indonesia. Upaya yang dapat dilakukan untuk mencegah kepunahan beruang madu adalah melalui konservasi. Kawasan Wisata Pendidikan Lingkungan Hidup (KWPLH) beruang madu di Balikpapan dinilai memiliki cara konservasi ex-situ yang terbaik di Asia. Penelitian ini bertujuan mengkaji cara konservasi beruang madu di KWPLH Balikpapan. Metode penelitian yang digunakan adalah metode survei langsung,  wawancara, dan analisis dokumen. Hasil penelitian menunjukkan bahwa secara fisik, ekologis, dan aspek sosial masyarakat, KWPLH beruang madu Balikpapan cukup baik sebagai habitat untuk konservasi beruang madu, sekaligus sebagai kawasan untuk pembelajaran lingkungan hidup. Cara konservasi ini layak digunakan sebagai model konservasi satwa liar yang lain. Saran yang dapat disampaikan adalah perluasan area enklosur beruang madu sesuai dengan daerah jelajahnya, sehingga diharapkan dapat bereproduksi secara normal. Dengan demikian keberhasilan konservasi secara ex-situ dapat dicapai. AbstractSun Bear (Helarctos malayanus) is one of the protected fauna legislation Indonesia. Efforts should be made to prevent the extinction of the sun bear is through conservation. Sun Bear Kawasan Wisata Pendidikan Lingkungan Hidup (KWPLH) in Balikpapan is considered have the best way of ex-situ conservation in Asia. This study aims to study how the sun bear conservation in KWPLH Balikpapan. The research method used are direct surveys, interviews, and document analysis. The results showed that physically, ecologically, and as society aspect, Sun Bear KWPLH Balikpapan is good enough as a habitat for the conservation of the sun bear, as well as the area for environmental learning. This conservation is feasible used as a wildlife conservation model. We suggest that the xpansion area of sun bear’s enclosure is feasible to its home range, so it is expected to have normal reproduction. Thus the success of ex -situ conservation can be achieved.
Improving Silvofishery Management Through Seedling Growth – Environment Quality Dynamic Relation Analysis Endah Dwi Hastuti; Rini Budi Hastuti
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.10490

Abstract

Mangrove plants are sensitive to environment condition. This research aimed to analyze the linkages of mangrove growth and environment dynamics and to estimate the growth of mangrove along with the environment dynamics. The research was conducted through the field experiment by the plantation of A. marina in silvofishery pond canals. Data collection was conducted for 18 months with 3 months observation interval. The environment variables observed including temperature, salinity, turbidity, pH, dissolved oxygen, TSS, sediment organic matter, nitrogen and phosphorus, and the growth of mangrove seedling. Analysis was conducted through regression and modelling with Powersim software. The result showed that the height growth was affected by dissolved oxygen, temperature, salinity, turbidity and pH, while the diameter growth was affected by TSS concentration. Inversely, the growth of mangrove also had a significant effect on temperature, change of organic matter and nutrient sediment concentration. Simulation showed that the height and diameter growth rates of A. marina seedling were dynamically changed among periods. Simulated seedling growth for one year forecast resulted in the rate of 0.115024 to 0.282294 cm/day for height and 0.001287 to 0.006031 cm/day for diameter. The simulation also indicated the continuous accumulation of organic matter and nitrogen over time. This research concluded that under limited environment dynamic, the growth of mangrove could be estimated. This model is a novelty in ecological studies. This research might initiates the more advanced ecological studies. Systematic estimation of ecosystem behaviour could be applied to formulate the best management practices, particularly in the silvofishery activities.
Species Richness and Birds Conservation Priority in Mount Rinjani Areas, Lombok Gito Hadiprayitno; I Gde Mertha; Moh. Liwa Ilhamdi
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.5039

Abstract

Mount Rinjani area on the island of  Lombok is unique in bioecology. It provides habitats for many species of birds, especially birds of Wallacea. Several studies  on birds in this area have been done, but the conservation priority of these birds has not been determined. Based on this condition, the purpose of this research is to determine the priority of birds conservation in mount Rinjani areas. Exploration about the spesies of birds was started from Senaru Village to Segara Anak Lake. Abundance of each bird species was estimated using point count. Data analysis to determine the conservation priority used indicators that consist of endemicity, population status, species threatened and species management. The results showed that the birds species in mount Rinjani  areas are composed of 32 species belonging to 20 families. Bird species that have high abundance were Amandava amandava (15.94%), Zosterops Montanus (15.70%), and Parus major (11.32%), while Ptilinopus melanospila and Anthus novaeseelandiae were in low abundance (0.23%). Analysis birds conservation priority  in mount Rinjani areas indicates that Otus jolandae has the highest score (65) and should be the first priority for the conservation, while Philemon buceroides and Gallus varius with the same score (60) are the second priority. How to CiteHadiprayitno, G., Mertha, I. G., Ilhamdi, M. L. (2016). Species Richness and Birds Conservation Priority in Mount Rinjani Areas. Biosaintifika: Journal of Biology Biology Education, 8(3), 270-277. 
Science Web-Module Integrated with Tannery Waste as Local Potential to Improve Students’ Problem Solving Atznain Iqma Miswami; Heru Nurcahyo
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.24356

Abstract

Problem solving skills are very important skill students must owned. The low score of students’ problem solving skills becomes a serious problem in the 21st century teaching and learning. The development of science web-based module integrated with tannery waste as local potential became a research that must be done. This research examined the appropriateness and the effectiveness of a science web-based module integrated with tannery waste as local potential in improving students’ problem solving skills. The Four-D research and development model was employed, consisting of define, design, develop, and disseminate. This research was conducted in the form of pretest posttest control group design. The appropriateness analysis conducted by content expert, teaching media expert, and science teacher indicating that the product was categorized very good. The effectiveness of the learning was tested using the independent sample t test with sig. value set at 0.000. The test showed that the science teaching done using the science web-based module was effective in improving students’ problem solving skills in SMP Negeri 1 Wungu. The product resulted from this study is expected to overcome the teaching and learning problems associated with the low score of students' problem-solving skills. Moreover, it can provide a real experience and independence to students in using the science web-based module to study.
Fruit Morphological Characteristics and β-carotene Content of Three Indonesian Dessert and Cooking Banana Cultivars Ari Sunandar; Dedeh Kurniasih
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 1 (2019): April 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v11i1.16873

Abstract

Approximately, 325 cultivars of bananas were found in Indonesia. They have variation in shape, taste, color of fruit pulp, essential vitamins and mineral. The β-carotene is one of essential nutrient in banana with various concentration in every cultivar. The aims of this research were to describe the morphological characteristics and to analyze β-carotene content of mature fruits of three different genomic groups of Indonesian dessert and cooking banana cultivars namely Berlin (AA/dessert), Barangan (AAA/dessert), and Kepok (ABB/cooking). Morphological characterizations of fruits were conducted according to Descriptor for Banana from International Plant Genetic Resources Institute. The β-carotene content of mature banana pulp was analyzed using High-performance liquid chromatography (HPLC) method with three replications. Banana fruit characters were analyzed based on 15 descriptors. The bananas were then subjected to clustering analysis.  The result showed that β-carotene content  were 27 μg 100g-1, 5 µg 100g-1, and 3 μg 100g-1 fresh weight respectively. Berlin showed the highest value of β-carotene. Cluster analyses formed two groups. The first group consists of Berlin and Barangan while the second one consists of Kepok. This study can be used as a a reference for people to consume bananas with high content of β-carotene in meeting the needs of vitamin A and as a consideration for the plant breeding especially for β-carotene improvement purpose.
Diversity in Tuber Characteristics of Local Cultivars of Yam Bean (Pachyrhizus erosus) in Indonesia Ayda Krisnawati; Sutrisno Sutrisno; Mochammad Muchlish Adie
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 2 (2018): August 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i2.14272

Abstract

Yam bean is legume with tuberous root originated from the semiarid tropics of Central America, and has been widely spread in several regions in Indonesia. Research on characterization of tuber yam bean was conducted in Kendalpayak Research Station (Malang, Indonesia) from May to October 2016. A total of 73 local yam bean cultivars originated from eight provinces were characterized based on their tuber characteristics. The sensory evaluation was also conducted to evaluate the tuber sweetness, fiber intensity, and texture crunchiness. The average tuber weight was 455.2 g/plant, with the average tuber length and width were 10.78 cm and 8.17 cm, respectively. The ratio of tuber length and width was 1.42, indicates that yam bean tuber in Indonesia tend to be in spherical shape. The average tuber water content was 83.87% (wet basis), showed that the water content was quite high. Based on cluster analysis, 74 local cultivars of yam bean were grouped into five clusters. The tuber weight varied among cultivars and become the distinguishing factor between local cultivars. Local variety of yam beans in Indonesia tend to have sweet taste and crunchy texture. So far, the study on characterization of tuber diversity in Indonesian local cultivar of yam bean is still limited. Thus, these results provide important information to optimize the tuber yam utilization as industrial raw materials.
Inventarisasi Jenis-Jenis Tumbuhan Berkhasiat Obat di Hutan Hujan Dataran Rendah Desa Nyamplung Pulau Karimunjawa Muhammad Abdullah; Dewi Mustikaningtyas; Talitha Widiatningrum
Biosaintifika: Journal of Biology & Biology Education Vol 2, No 2 (2010): September 2010
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v2i2.1153

Abstract

The research aims to obtain information about the various of medicinal plantsspecies. from 17 species of wild plants were found, approximately 76.5% (13)has been known to have the potential to be used as a medicine, namely jambumete, kecapi, gondang putih, laban, petai cina, jati, gerok ayam, lampeni, jemang,mangga, beruas, karet manis, and kopi anjing.Keywords: medicinal plant, lowland rain forest, Karimunjawa
Traditional Usages of Taro (Colocasia spp.) by Ethnic Communities in Borneo Linda Oktavianingsih; E. Suharyanto; Budi Setiadi Daryono; Purnomo Purnomo
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i2.9956

Abstract

Borneo has a a wealth of various flora, including the Aroids (Araceae). Taro is one of the Aroids that cultivated and used as food crop since a long time ago by the people in Borneo. This study aimed to determine the utilization of Taros traditionally by several ethnic communities in Borneo. The research used Survey Explorative Method with Direct Interview Technique in the field. Taro samples were taken from various habitats of Banjar, Dayak, Kutai, Malay, Bugis, Toraja and China ethnic. The results showed that Taro species widely used are : Colocasia esculenta var. esculenta and var. antiquorum, Colocasia  affinis and wild of Colocasiaesculenta. C. esculenta var. esculenta and var. antiquorum is used as subsistence food crop and vegetables. Besides, it is used as medicine such as high blood pressure lowering and for consumption of diabetics. Parts of the plants consumed include leaves, petiole, corm and stolon. C. affinis is used as ornamental plants of the home garden, while wild C. esculenta  is used as animal fodder. Taro in Borneo have a considerable variation of traditional cultivars and vernacular names. Fifty eight traditional cultivars from 5 different habitat to be used by some ethnics.

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