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Biosaintifika: Journal of Biology & Biology Education
Published by Universitas Negeri Semarang
ISSN : 2085191X     EISSN : 23387610     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Biosaintifika Journal of Biology & Biology Education, an electronic international journal, provides a forum for publishing the original research articles, review articles from contributors, and the novel technology news related to biological research and biological education.
Arjuna Subject : -
Articles 1,068 Documents
 88   89   90   91   92 
EFEK PERENDAMAN INFUSA DAUN SALAM (Syzygium polyanthum) TERHADAP KUALITAS DAGING AYAM POSTMORTEM Feri Dwi Agustina; Priyantini Widiyaningrum; Ari Yuniastuti
Biosaintifika: Journal of Biology & Biology Education Vol 4, No 2 (2012): September 2012
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v4i2.2271

Abstract

Tujuan penelitian ini untuk mengevaluasi efek perendaman daging ayam broiler dalam konsentrasi infusa daun salam yang berbeda. Penelitian menggunakan desain faktorial dengan 3 taraf konsentrasi infusa (0%; 5%; dan 10%) serta dua waktu pengamatan yaitu 0 dan 8 jam setelah perlakuan perendaman. Parameter yang diukur meliputi rata-rata bilangan peroksida, pH, kadar air dan water holding capacity (WHC). Data dianalisis menggunakan analisis varian dan uji perbedaan akibat perlakuan dilakukan dengan uji beda nyata terkecil. Hasil penelitian menunjukkan bahwa pengukuran pH dan WHC sesaat setelah perlakuan memperlihatkan perbedaan nyata (P0,05), sementara bilangan peroksida dan kadar air tidak berbeda nyata. Pada 8 jam setelah perendaman, terlihat bahwa nilai peroksida, pH, kadar air dan WHC menunjukkan perbedaan nyata. Penelitian ini menyimpulkan bahwa infusa daun salam mampu memperlambat laju bilangan peroksida, memperlambat penurunan pH dan memperlambat penurunan WHC.The aim of this study was to evaluate the effects of the immersion of chicken meat in the bayleaf extract and the time of measurement on the quality of the meat. This was a factorial experiment with three levels of bayleaf extract concentrations (0%, 5% and 10%), and two time of measurement (at 0 and 8 hours after the treatment). The parameters observed were the average peroxide value, the pH, the water content and the water holding capacity. The data were analyzed using ANOVA and followed by the least significant difference test for any significant difference. The results showed that the pH and the water holding capacity were significantly different (P0.05), whereas the peroxide value and the water content were not significantly different. Nevertheless, at eight hours after the immersion it was seen that the peroxide value, the pH, the water content and the water holding capacity were significantly different. It was concluded that the extract of bayleaves might slower the rate of the peroxide value, could decrease the pH, and decrease the water holding capacity.
Correlation of Physical-Chemical Parameters to Total Coliform Value in Jawi River, Pontianak, West Kalimantan Rahmawati Rahmawati; Laili Fitria; Liza Syafitri
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i2.10187

Abstract

Coliform bacteria can be used as an indicator of the presence of pathogenic bacteria, such as E.coli bacteria that cause diarrhea. The aimed of this study is to determine the relationship between physical-chemical parameters namely temperature, pH, DO and BOD to the density of coliform bacteria in Jawi River, Pontianak. The sampling was conducted at one point each in the upstream, midstream and downstream area of the Jawi River during two tidal conditions of the Kapuas River in September 2016 at 09:40 (at low tide) and at 15:40 (at high tide). The correlation of physical-chemical parameter to coliform value was tested Pearson Product Moment. The results showed that coliform bacterial density increased from upstream to downstream with 150-1500 MPN/100 ml at high tide and 930-11000 MPN/100 ml at low tide. The results showed that the coliform bacterial density value had a positive relation with pH and BOD parameters and negative relation with temperature and DO parameters. So, it can be concluded that there is a correlation between physical parameters, such as temperature and chemical parameters such as pH, DO and BOD to microbiological parameters especially Coliform bacterial density. The benefit of this study is to give information about water quality of Jawi River and its correlation with density of Coliform bacterial, so that people are expected to pay more attention to the use of clean water to avoid the disease caused by coliform.
Antioxidant Activity from Various Tomato Processing Retno Sri Iswari; R Susanti
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 1 (2016): March 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i1.4722

Abstract

Tomato is one of the high antioxidant potential vegetables. Nowadays, there are many techniques of tomato processings instead of fresh consumption, i.e. boiled, steamed, juiced and sauteed. Every treatment of cooking will influence the chemical compound inside the fruits and the body's nutrition intake. It is important to conduct the research on antioxidant compound especially lycopene, β-carotene, vitamin C, α-tocopherol, and its activity after processing. This research has been done using the experimental method. Tomatoes were cooked into six difference ways, and then it was extracted using the same procedure continued with antioxidant measurement. The research results showed that steaming had promoted the higher antioxidant numbers (lycopene. α-tocopherol, β-carotene and vitamin C) and higher TCA and antioxidant activities in the tomatoes than other processings. It was indicated that steaming was the best way to enhance amount, capacity and activities of antioxidants of the tomatoes.
Candida Species Distribution of Clinical Specimens in Banda Aceh, Indonesia Suhartono Suhartono; Wilda Mahdani; Aderiana Masthura; Iman Rusmana
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 2 (2020): August 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i2.23758

Abstract

Candidiasis has become increasingly widespread in the community alongside with the developing resistance of Candida sp. to some antifungals. A prevalence study in the present research is required to surveil the distribution of Candida-related infections to administrate the appropriate antifungal treatments. The objective of this research was to determine the species distribution of candidiasis with their antifungal susceptibility isolated from clinical specimens at the Zainoel Abidin Hospital (ZAH) Banda Aceh, Indonesia. The clinical specimens were from inpatients and outpatients in the hospital during January to June 2019. The identification of Candida species and antifungal sensitivity assay were conducted by using VITEK® 2 Compact. Of a total of 68 isolates, there were six species of Candida with the highest species prevalence was Candida tropicalis (52.94%). Additionally, the highest prevalence of candidiasis came from urine specimen (54.41%) and mostly from inpatients in the internal medicine unit (54.41%). Candidiasis occurred predominantly in men (58.82%) and during adulthood (55.88%). The antibiogram of Candida sp. shows a high percentage of sensitivity towards some antifungals including fluconazole (100%), voriconazole (100%), caspofungin (100%), micafungin (100%), amphotericin B (99.5%), and flucytosine (100%). From this result, it can be concluded that candidiasis cases in the ZAH Banda Aceh, Indonesia has prevalently occurred with the few antifungal therapies for candidiasis of were still effective empirically and definitively. This is an initial study of Candida prevalence within different clinical samples in Banda Aceh and the study is expected to be a basis for prevention and control of Candida-related infections in the area.
Antioxidant Effect of Chlorella vulgaris on Physiological Response of Rat Induced by Carbon Tetrachloride Hernayanti Hernayanti; Sorta Basar Ida Simanjuntak
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 1 (2019): April 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v11i1.16393

Abstract

Chlorella vulgaris is an algae with high nutrition content. Carbon tetrachloride (CCl4) is a hepatotoxic chemical.The aims of this study were to determine the effect of C. vulgaris extract on the physiological response of liver such as MDA, SOD and GPx activity on rat after induced by CCl4 exposure as well as to determine the effective dose of C. vulgaris  extract as antioxidant that can neutralize CCl4 exposure.  This research was conducted experimentally with Completely Randomized Design that consists of 6 treatment and 5 times repetition. The doses of C. vulgaris extract used were 3, 4, and 5 mg per /100 g of rat’sbody weight (BW).The administration of C. vulgaris extract was performed within 30 days, while the CCl4 (0.25 ml/100 g BW) was administered orally on the day 9, 12, 16, 19, 23, and 26. Parameters measured were levels of MDA, SOD and GPx of rat blood serum.The results showed that the administration of C. vulgaris extract can inhibit lipid peroxidation indicated by decrease in MDA activities and oxidative stress by increasing SOD and GPx activity. In conclusion, 5 mg/100 g BW of C.vulgaris extract is an effective dose to be uses as endogenous antioxidant to protect the liver cell from damage caused by CCl4exposure. The benefit of C. vulgaris as a supplement for antihepatotoxin in humans.
The Structural Resistance’s Anatomy of Sweet Potato Leaves to Fungal Pathogen Sphaceloma batatas Siti Samiyarsih; Juwarno Juwarno; Juni Safitri Muljowati
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 1 (2018): April 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i1.12116

Abstract

Anatomical characters can be used as instructions to the structural resistance of plants to pathogen attack. Various pathogens attack sweet potato plants, such as the Sphaceloma batatas fungus that causes scurvy disease (scab). The aims of this research t test the structural resistance of sweet potato plants based on leaf anatomical character and intensity of disease attack due to the inoculated of S. batatas. The research was conducted from June to November 2016, using the Completely Randomized Design Experimental (RAL) method with a factorial pattern. The first factor was ten sweet potato cultivars and the second factor was the inoculum of S. batatas fungus each treatment with five replications. The character of leaf anatomy observed was thick of cuticle, thick of mesophyll, size and number of stomata and number of trichomes per 1 mm2 leaf area. Based on the research result, it was concluded that the inoculation of the fungus of S. batatas caused the decrease of stomata length and width on ten sweet potato cultivars. The highest intensity of disease attack was 14.33% and correlated with stomata length (r = 0.49). The anatomical structural resistance to scurvy can be used as a basis for determining crosses for obtaining superior sweet potato cultivars. The benefit of the research is to advise the community to cultivate sweet potatoes that have structural resistance to scurvy, such as cuticle and thick mesophyll, high trichomata density.
Nutrient Uptake, Chlorophyll Content, and Yield of Rice (Oryza sativa L.) Under the Application of PGPR Consortium Purwanto Purwanto; Woro Sri Suharti
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 3 (2021): December 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i3.31990

Abstract

Indigenous paddy soil rhizobacteria are one alternative to restore biological fertility and soil health. Plant Growth Promotion Rhizobacteria (PGPR) that act as biofertilizer will help to increase the availability of nutrients and promote the plant growth. The objective of this research was to study the effect of the PGPR consortium indigenous paddy soil to nutrient uptake, chlorophyll content, and yield of rice. This research was arranged by Randomized Block Design with the treatment was the combination of plant Growth Promotion Rhizobateria isolates originated from paddy soil. The treatments consisted of control, Rhizobium sp. LM-5, R08 isolate, R011 isolate, Rhizobium sp. LM-5 + R08 isolate, Rhizobium sp. LM-5 + R011 isolate, R08 + R011 isolates, Rhizobium sp. LM-5 + R08 + R011 isolates. The result showed that the application of the PGPR consortium was able to increase the root growth of rice plants thereby increasing nutrient uptake, leaf chlorophyll content, and plant biomass. Application of single strain R11 isolate and the consortium of Rhizobium sp. LM-5 + R11 isolate were capable of giving the highest grain yield of 64.99 and 62.80 g plant-1 respectively. These finding were PGPR consortium between IAA-producing bacteria combined with Rhizobium sp. LM-5 as N2 fixing bacteria in increasing nutrient uptake, chlorophyll contents and crop yields, it can be recommended that PGPR consortium as a biofertilizer formula in rice cultivation
Keanekaragaman Genetika Pisang Bergenom B Berdasarkan Penanda Mikrosatelit Windarti Windarti
Biosaintifika: Journal of Biology & Biology Education Vol 1, No 1 (2009): March 2009
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v1i1.34

Abstract

The morphology and isozyme marker was not enough to reveal banana genetic diversity. So, it needs molecular analysis. The purpose of this research is to know the genetic diversity of banana with B genome using microsatelite marker. Forty bananas with B genom from Yogyakarta Agriculture Service and Animal Husbandary Service and also from Tropical Fruit Centre of Bogor Agriculture Institute were amplified using 6 loci (MaCIR327b, MaCIR108, Ma-3-139, Ma-3-90, Ma-1-17, and Ma-1-27). The diversity were identified with allele amount, genotype, observe heterozigosity, and filogeny. There are eight allele per locus average from six microsatelites. MaCIR108 produced the highest allele amount, there are 13 alleles. The research identified 75 genotypes. MaCIR327b, MaCIR108, Ma-3-139, Ma-3-90, Ma-1-17, and Ma-1-27, respectively, produced 8, 20, 13, 14, 15, and 5 alleles. The Ho average is quite high, there are 0,77. The similarity coefficient is between 0,597 – 1,00. MaCIR108 and Ma-3-90 can be used to identify banana group genom. As a group genom characteristic, Ma-1-17 needs more research. Identification result between morphology and microsatelite marker were different. Based on morphology marker, Sri Wulan and Byar cultivar were ABB, and Ketip Gunung Sari were AAB, but based on microsatelite marker Sri Wulan and Byar cultivar were AAB, while Ketip Gunung Sari has not B on its genom. Keywords: genetic diversity, banana, microsatelit.
Ethnobotany of Bamboo in Sangirese, North Celebes Alin Liana; Purnomo Purnomo; Issirep Sumardi; Budi Setiadi Daryono
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 1 (2017): April 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i1.7405

Abstract

Advances in technology have encouraged the growth of a variety of biodiversity processing methods. These conditions encourage efforts to use natural resources more broadly. Ethnobotany can be used to document the local knowledge of the plants and their utilization. This study is aimed to describe the various forms of bamboo utilization by Sangirese. The method used was survey with purposive sampling. The data were analyzed descriptively based on exploration results and interviews with the people. The results showed there were 11 species of bamboo that are directly related to the life of local community in Sangirese, consisting of seven species of Bambusa, three species of Schizostachyum, and each one species of Dendrocalamus and Gigantochloa. Local community use bamboo for a variety of daily necessities; as building materials, handicraft, furniture, ceremonies, musical instruments, transportation, medicine, ornamental plants, as well as food. These exploration results are expected to complement the data of bamboo ethnobotany in Indonesia. Applicatively, this research is expected to be a reference in the national strategy of bamboo germplasm resource conservation.
Pengaruh Berbagai Jenis Umpan Semut terhadap Kesintasan Undur-Undur Pradyna Niata WA; Sri Ngabekti; Partaya Partaya
Biosaintifika: Journal of Biology & Biology Education Vol 5, No 2 (2013): September 2013
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v5i2.3915

Abstract

Pada saat ini undur-undur banyak dicari oleh manusia karena mengandung zat anti diabetes. Jika tidak ada semut yang terjebak di lubang undur-undur, maka undurundur tidak akan memperoleh makanan yang dikhawatirkan menurunkan kesintasan undur-undur. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh berbagai jenis umpan semut terhadap kesintasan undur-undur. Jenis bahan makanan yang dapat mengundang semut yang digunakan pada penelitian ini yaitu berupa air gula, sirup dan kulit udang. Kesintasan hidup undur-undur diamati selama 3 bulan. Data kesintasan hidup undur-undur dianalisis dengan menggunakan Anava Dua jalur serta berat dan panjang undur-undur dianalisis dengan t-test. Hasil penelitian menunjukkan bahwa kesintasan undur-undur terjadi pada semua perlakuan yakni pada umpan semut yang berupa air gula, sirup dan kulit udang, hal ini ditunjukkan dengan jumlah undur-undur yang hidup hingga akhir penelitian sebanyak 93-97%, pada kelompok tanpa perlakuan (alami) kesintasannya hanya 80%. Umpan semut kulit udang mendatangkan 2 jenis semut sedangkan umpan semut air gula dan sirup hanya mendatangkan masing-masing satu jenis semut. Semut tersebut menjadi makanan undur-undur dan meningkatkan kesintasan dan pertumbuhan undur-undur. Dari hasil penelitian dan pembahasan dapat disimpulkan bahwa pemberian umpan semut sebagai makanan undur-undur dapat meningkatkan kesintasan undur-undur.Gratus much sought after by humans at this time because it contains anti-diabetic agent. If there are no ants are trapped in the hole gratus, then gratus will not get food that may reduce survivorship gratus. The aim of this research is to to determine the effect of various types of ant bait to survivorship gratus. Types of food that can invite ants used in this research is in the form of sugar water, syrup and shrimp shells. Survivorship life gratus observed for 3 months. Data survivorship life gratus analyzed using ANAVA Two long lines and, heavy and gratus analyzed by t-test. The results showed that the survival rate gratus occurred in all treatment that is on the ant bait in the form of sugar water, syrup and shrimp shells. This is indicated by the number gratus living up to the end of the study as much as 93-97%, in the group without treatment (natural) survival rates of only 80%. Ant bait shrimp shells bring two types of ants ant bait water while sugar and syrup only bring one of each type of ant. The ants feed gratus and enhances survival and growth gratus. From the results of research and discussion can be concluded that feeding ants as food gratus can increase survivorship gratus.

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