cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
Ilham
Contact Email
Ilham.fishaholic@gmail.com
Phone
+6221-64700928
Journal Mail Official
jra.puslitbangkan@gmail.com
Editorial Address
Gedung Balibang KP II, Lantai 2 Jl. Pasir Putih II, Ancol Timur, Jakarta Utara 14430
Location
Kab. jembrana,
Bali
INDONESIA
Jurnal Riset Akuakultur
Published by Politeknik Kelautan dan Perikanan Jembrana
ISSN : 19076754     EISSN : 25026534     DOI : http://doi.org/10.15578/JRA
Core Subject : Agriculture, Social,
Agriculture, Biological Sciences & Forestry Environmental Science
Jurnal Riset Akuakultur as source of information in the form of the results of research and scientific review (review) in the field of various aquaculture disciplines include genetics and reproduction, biotechnology, nutrition and feed, fish health and the environment, and land resources in aquaculture
Arjuna Subject : Umum - Umum
Articles 10 Documents
 1 
Search results for , issue "Vol 11, No 2 (2016): (Juni 2016)" : 10 Documents clear
PERFORMA BENIH TERIPANG PASIR, Holothuria scabra DARI SUMBER INDUK YANG BERBEDA Sari Budi Moria Sembiring; Ida Komang Wardana; Haryanti Haryanti
Jurnal Riset Akuakultur Vol 11, No 2 (2016): (Juni 2016)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (579.708 KB) | DOI: 10.15578/jra.11.2.2016.147-154

Abstract

Upaya pengembangan perbenihan teripang pasir bagi kelestarian populasi di alam dan pengembangan budidaya patut dilakukan. Upaya ini diperlukan mengingat semakin intensifnya penangkapan teripang di alam yang dapat menimbulkan terganggunya kelestarian populasi ini. Tujuan dari penelitian ini adalah untuk mengevaluasi performa pertumbuhan benih teripang pasir, mendapatkan informasi keragaman genetik dan mengestimasi laju inbreeding dari 3 sumber induk teripang yang berbeda. Tiga sumber induk berasal dari perairan Bali, Sulawesi Selatan, dan Maluku Tenggara masing-masing sebanyak 20 ekor dianalisis menggunakan mikrosatelit (SSR/Simple Sequence Repeats) dengan 3 lokus, yaitu Hsc-28; Hsc-49 dan Hsc-59. Proses pembenihan mengikuti pedoman teknis yang sudah ada dengan beberapa modifikasi. Hasil yang diperoleh menunjukkan bahwa panjang dan bobot benih umur 6 bulan yang dihasilkan dari induk Maluku Tenggara relatif lebih tinggi (5,67 ± 0,76 cm; 13,26 ± 5,63 g) dibandingkan dengan benih dari induk Sulawesi Selatan (4,75 ± 0,91 cm; 6,3 ± 2,22 g) dan Bali (4,85 ± 0,64 cm; 6,2 ± 3,6 g). Hasil analisis mikrosatelit menunjukkan bahwa keragaman genetik induk teripang pasir dari ke tiga populasi tidak berbeda nyata. Hal ini berdasarkan nilai differensiasi genetik (FST= 0,2475 atau 24,75%). Laju nilai inbreeding dalam populasi induk teripang pasir cukup tinggi (FIT= 0,4237 atau 42,37%) dibandingkan dengan laju inbreeding antar populasi (FIS) adalah 0,2342 atau 23,42%. The effort of sea cucumber seed production for culture development have to be carried out. This effort is also required due to the intensive exploration of sea cucumber in the nature which could threaten of its sustainability. The aims of this research is to evaluate sea cucumber juveniles growth performance, to obtain the information on genetic variation, and to estimate the rate of inbreeding from three different sea cucumber broodstock sources. Three sources of sea cucumber were collected from Bali, South Sulawesi, and Southeast Moluccas. The total of 20 pcs from each area were analyzed by microsatellite (SSR Simple Squence Repeat) with 3 locus namely Hsc-28; Hsc-49 dan Hsc-59. Hatchery production of sea cucumber seed followed the existing manual with some modifications. Result of the experiment showed that the length and weight of 6 months old juveniles produced by Southeast Moluccas’s broodstock were relatively higher (5.67 ± 0.76 cm; 13.26 ± 5.63 g) compared to the juveniles produced by South Sulawesi’s broodstock (4.75 ± 0.91 cm; 6.30 ± 2.22 g) and Bali’s broodstock ( 4.85 ± 0.64 cm; 6.2 ± 3.6 g). Results of microsatellite analysis showed that genetic variation of the three broodstock populations was not significant different. It is based on the genetic differentiation value (FST= 0.2475 or 24.75%. The result of inbreeding rate within the broodstock of sea cucumber population was high (FIT= 0.4237 or 42.37%) compare to the rate value of inter population (FIS= 0.2342 or 23.42%.
EVALUASI PEMBERIAN PROBIOTIK BACILLUS PADA MEDIA PEMELIHARAAN TERHADAP LAJU PERTUMBUHAN DAN PERUBAHAN HISTOPATOLOGI IKAN LELE DUMBO (Clarias gariepinus) YANG DIINFEKSI Aeromonas hydrophila Angela Mariana Lusiastuti; Mohammad Faizal Ulkhaq; Widanarni Widanarni; Tri Heru Prihadi
Jurnal Riset Akuakultur Vol 11, No 2 (2016): (Juni 2016)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (665.736 KB) | DOI: 10.15578/jra.11.2.2016.171-179

Abstract

The aim of this study was to evaluate the addition of a probiotic Bacillus on culture medium through growth rate and histopathological change in African Catfish who was infected by Aeromonas hydrophila. The study consisted of five treatments such as the addition of probiotic Bacillus ND2, Bacillus P4I2, Bacillus ND2 + Bacillus P4I2 (Kom), positive control (K+) and negative control (K-) (without probiotic addition). African Catfish (13.354±2.8 g) was maintained in 15 aquariums (40 L in volume) with 30 fishes each for 30 days. Probiotic bacteria was applied in water once a day, whereas pathogenic bacteria A. hydrophila  (103 CFU/mL) were added once in earlier treatment (except for the negative control) as assumption that A. hydrophila will shape up quorum sensing in unfortunate condition. The result showed that the highest spesific growth rate in the treatment of Bacillus ND2 probiotics (1,708 ± 0.114%). Histopathology showed that damaged of liver dan kidney in probiotics treatment were lightner than positive control (K+).  The addition of Bacillus P4I1 (104 CFU/mL) efective to increased the spesific growth rate of African Catfish and prevent the damage of liver and kidney of African Catfish was infected by Aeromoniasis.
KARAKTERISASI EMPAT POPULASI IKAN GURAMI (Osphronemus goramy Lac.) DAN PERSILANGANNYA BERDASARKAN METODE TRUSS MORFOMETRIKS Suharyanto Suharyanto; Rita Febrianti; Sularto Sularto
Jurnal Riset Akuakultur Vol 11, No 2 (2016): (Juni 2016)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (545.575 KB) | DOI: 10.15578/jra.11.2.2016.125-135

Abstract

Tahap awal yang dilakukan dalam rangka pembentukan populasi ikan gurami cepat tumbuh adalah koleksi dan karakterisasi populasi-populasi ikan gurami yang akan digunakan sebagai sumber genetik pembentukan varietas tersebut. Kegiatan ini dilakukan untuk mengevaluasi keragaman morfologi dan hubungan kekerabatan empat populasi ikan gurami, yaitu Jambi (J), Kalimantan Selatan (K), Majalengka (M), dan Tasikmalaya (T). Metode truss morfometrik digunakan untuk karakterisasi morfologi dilanjutkan dengan analisis komponen utama (principal component analysis) dan analisis pengelompokan (cluster analysis). Hasil karakterisasi menunjukkan bahwa diagram pencar populasi ikan gurami tanpa melihat jenis kelamin menunjukkan adanya pengelompokan populasi menjadi dua kelompok, yaitu kelompok pertama adalah persilangan JxM dan MxK, sedangkan kelompok kedua terdiri atas persilangan JxK, KxJ, TxJ, KxM, MxJ, KxT, galur murni KxK, JxJ, MxM, dan TxT. Hal tersebut terjadi pula pada populasi jantan. Populasi betina menunjukkan JxK dan MxK terpisah berdasarkan karakter A2 (dahi-pangkal sirip punggung) dan A3 (pangkal sirip punggung-pangkal sirip perut). Indeks kesamaan tertinggi dalam 12 populasi diperoleh pada populasi Jambi dan Majalengka berturut-turut sebesar 94,00% dan 92,00%; sedangkan indeks kesamaan terendah diperoleh pada populasi TJ sebesar 72,00%. Ikan gurami ukuran konsumsi terdapat empat kelompok besar berdasarkan bentuk badannya. Dua kelompok pada galur murni menunjukkan populasi galur murni Kalimantan, Majalengka, dan Tasikmalaya kekerabatannya dekat, akan tetapi dengan Jambi memiliki kekerabatan yang jauh. Dua kelompok lainnya pada populasi persilangan, yaitu: persilangan JxM dan MxK dan kelompok lainnya adalah persilangan KxJ, KxM, JxK, TxJ, MxJ, dan KxT. Populasi galur murni dan persilangan memiliki jarak genetik yang jauh, sehingga populasi galur murni dan persilangan itu berbeda.The first step in breeding program towards generating fast-growing strain of giant gourami is the collection and characterization of giant gourami populations have been used as a genetic source. Giant gourami had been collected from South Kalimantan, Jambi, Majalengka, and Tasikmalaya. The aim of this experiment was to determine the morphological diversity among these collected populations using truss morphometric method. Principal component analysis followed by cluster analysis were used to identify the pattern of morphological variability among populations and varieties. The results showed that dendrogram populations of giant gourami regardless of gender showed a grouping of some of the population into two groups: the first group was J×M and M×K crosses, while the second population consisted of: Jambi Kalimantan (J×K), Kalimantan Jambi (K×J), Tasikmalaya Jambi (T×J), Kalimantan Majalengka (K×M), Majalengka Jambi (M×J), Kalimantan Tasikmalaya (K×T), purebred Kalimantan (K×K), Jambi (J×J), Majalengka (M×M), and Tasikmalaya (T×T). This was true for the male population. Female population showed J×K and M×K apart, the difference lies in the character of the forehead-base of the dorsal fin (A2) and the base of the dorsal fin-fin base stomach (A3). The highest similarity index was found Jambi (94.00%) Majalengka (92.00%) populations, while the lowest similarity index was T×J (72.00%). At market size of the consumption of giant gourami there are four major groups, based on the shape of the body. Two groups on pure strains showed a population of pure lines Kalimantan, Majalengka, and Tasikmalaya close kinship, but Jambi had a distant kinship. Two other groups in the population crosses, namely: cross J×M and M×K and the other group was a cross K×J, M×K, J×K, T×J, M×J, and K×T. The population of pure lines and crosses had a genetic distance away, so that the population of pure lines and crosses were different.
KETERKAITAN SPASIAL KUALITAS LINGKUNGAN DAN KEBERADAAN FITOPLANKTON BERPOTENSI HABs PADA TAMBAK EKSTENSIF DI KECAMATAN LOSARI KABUPATEN CIREBON, JAWA BARAT Tarunamulia Tarunamulia; Kamariah Kamariah; Akhmad Mustafa
Jurnal Riset Akuakultur Vol 11, No 2 (2016): (Juni 2016)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (498.662 KB) | DOI: 10.15578/jra.11.2.2016.181-195

Abstract

Harmful Algal Blooms (HABs) dapat memberikan dampak negatif secara ekologis, ekonomis dan kesehatan.  Kejadian dapat bervariasi menurut faktor lingkungan lokal pemicu serta kemampuan adaptasi spesies.  Penelitian ini bertujuan untuk mengetahui keterkaitan antara karakteristik kualitas lingkungan dengan keberadaan fitoplankton berpotensi HABs pada tambak ekstensif di Kecamatan Losari Kabupaten Cirebon, Jawa Barat.  Sebanyak masing-masing 45 contoh air dan tanah diambil pada total luas petakan tambak ±2300 ha dengan metode transek yang dimodifikasi. Peubah kualitas air yang diukur meliputi; Total Amonia Nitrogen (TAN), Nitrit (NO2-N), Nitrat (NO3-N), Fosfat (PO4-P), Bahan Organik Total (BOT) dan Plankton.  Sedangkan peubah kualitas tanah tambak meliputi pH, total nitrogen (NTOT), fosfat (PO4-P) dan BOT. Analisis keterkaitan kualitas lingkungan dengan keberadaan fitoplankton berpotensi HABs dilakukan dengan BIO-ENV analysis, Cluster analysis, dan analisis spasial dengan software PRIMER 5.0 dan ArcGIS 10.0.  Dari  23 spesies yang diidentifikasi terdapat 5 spesies (21%) yang potensial sebagai HABs meliputi Prorocentrum sp, Ceratium sp, Gymnodinium sp, Thalassiosira sp dan Nitzchia sp.   Prorocentrum sp ditemukan pada 21 stasiun  dari total 45 stasiun dengan kepadatan tertinggi (508 ind/L). Hasil analisis selanjutnya menunjukkan bahwa distribusi spasial spesies berkaitan erat dengan distribusi nilai TAN dan BOT air serta nilai N-Total tanah. Jika tidak ada upaya pengelolaan dan mitigasi sehubungan keberadaan HABs tersebut maka dikhawatirkan dapat mempengaruhi produktivitas dan keberlanjutan kegiatan budidaya di lokasi penelitian.Harmful Algal Blooms (HABs) can cause serious negative ecological, economical and human health impacts. The occurrence of HABs may vary according to local environmental factors and the adaptability level of the causative species. This study aims to determine the relationship between environmental quality and the presence of causative phytoplankton species of HABs at extensive brackishwater aquaculture ponds located in Losari District, Cirebon Regency, and West Java Province. The sampling method followed a modified transect method by which a total of 45 each water and soil samples were taken from pond units, covering the total area of about 2300 ha. Water quality parameters comprised total ammonia nitrogen (TAN), nitrite, nitrate, phosphate and total organic matter (TOM). Whilst the pond soil quality variables included pH, total nitrogen (NTOT), phosphate and TOM. Spatial relationship between environmental quality and the presence of potentially causative phytoplankton species of HABs conducted through BIO-ENV analysis, cluster analysis and spatial analysis with the help of software PRIMER 5.0 and ArcGIS 10. Of the total 23 identified phytoplankton species, 5 species (21%) were classified as potentially causative sepecies of HABs including Prorocentrum sp, Ceratium sp, Gymnodinium sp, Thalassiosira sp and Nitzchia sp.  Prorocentrum sp was discovered in 21 stations of a total of 45 stations and accounted for the highest density (508 ind. / L). The results further indicated that the spatial distribution of the causative species is closely related to the distribution of values of TAN and TOM (water) and NTOT (soil).  Unless effective management and mitigation efforts are undertaken, the presence of the potentially causative species could affect the sustainability of aquaculture activities at the study sites.
KERAGAMAN GENOTIPE TIGA GENERASI IKAN RAINBOW KURUMOI (Melanotaenia parva) HASIL DOMESTIKASI BERDASARKAN RAPD; Genotype Diversity in Three Generations of Domesticated Kurumoi Rainbow Fish (Melanotaenia parva) Based on RAPD Method. Erma Primanita Hayuningtyas; Tutik Kadarini
Jurnal Riset Akuakultur Vol 11, No 2 (2016): (Juni 2016)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1272.838 KB) | DOI: 10.15578/jra.11.2.2016.107-114

Abstract

Ikan rainbow Kurumoi (Melanotaenia parva) adalah ikan endemik Danau Kurumoi, Papua Barat, Indonesia. Balai Penelitian dan Pengembangan Budidaya Ikan Hias (BPPBIH), Depok telah berhasil melakukan domestikasi dan menghasilkan beberapa generasi ikan rainbow Kurumoi. Ikan rainbow Kurumoi memijah secara alami, sehingga kemungkinan terjadinya inbreeding tinggi. Oleh karena itu, sangatlah penting untuk mengevaluasi keragaman genetik ikan rainbow Kurumoi. Penelitian ini bertujuan untuk mengetahui kualitas genetik dengan mengevaluasi keragaman genotipe tiga generasi ikan rainbow Kurumoi menggunakan metode RAPD (Randomly Amplified Polymorphic DNA) dengan tiga jenis primer, yaitu: OPA-18, OPZ-5, dan OPZ-13. Setiap generasi diambil 10 ekor ikan secara acak. Hasil penelitian ditemukan bahwa keragaman genotipe ikan generasi pertama (69,25%) relatif lebih rendah (P>0,05) daripada generasi kedua (76,9%) dan ketiga (76,9%). Nilai heterozigositas cenderung meningkat dari generasi ke generasi. Heterozigositas ikan generasi pertama adalah 0,21; generasi kedua sebesar 0,24; dan generasi ketiga sebesar 0,25. Jarak genetik terjauh adalah antara generasi pertama dan generasi ketiga, yaitu sebesar 0,19. Dengan demikian, proses domestikasi yang telah dilakukan tidak menyebabkan penurunan keragaman genotipe ikan rainbow Kurumoi.Kurumoi rainbow fish (Melanotaenia parva) is an endemic species from Kurumoi Lake, West Papua, Indonesia. Research and Development for Ornamental Fish Culture, Depok has successfully domesticated and produce Kurumoi rainbow fish for several generations. This fish is breed naturally, inbreeding probability is highly occure. It is important to evaluate genetic diversity of Kurumoi rainbow fish. Aim of the research was to evaluate genotype diversity at three generations of Kurumoi rainbow fish using RAPD (Randomly Amplified Polymorphic DNA) method with three primers, namely OPA-18, OPZ-5, and OPZ-13. In the research, 10 fishes were randomly taken from each generation. The research found that genotype diversity of fish first generation (69.25%) was relatively lower (P>0.05) than second (76.9%) and third (76.9%) generations. Heterozygosity value tended to increase by generation to generation. Heterozygosity at first generation was 0.21, 0.24 at second generation and 0.25 at third generation. The highest genetic distance was between the first and third generation (0.19). Thus, the domestication process that has been done does not cause a decrease in genotype diversity of Kurumoi rainbow fish.
PEWARISAN MARKA Cyca-DAB1*05 DAN KERAGAMAN GENETIK IKAN MAS (Cyprinus carpio) STRAIN RAJADANU TAHAN INFEKSI KOI HERPESVIRUS DAN TUMBUH CEPAT Khairul Syahputra; Yogi Himawan; Didik Ariyanto; Flandrianto S. Palim
Jurnal Riset Akuakultur Vol 11, No 2 (2016): (Juni 2016)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (212.382 KB) | DOI: 10.15578/jra.11.2.2016.115-123

Abstract

Balai Penelitian Pemuliaan Ikan (BPPI) telah membentuk ikan mas Rajadanu tahan infeksi koi herpesvirus (KHV) dan tumbuh cepat melalui program seleksi. Ikan mas Rajadanu dihasilkan dari kegiatan seleksi bersamaan pada karakter ketahanan terhadap KHV dan pertumbuhan. Seleksi karakter ketahanan terhadap KHV dilakukan dengan menggunakan marka Cyca-DAB1*05 dan seleksi karakter pertumbuhan cepat dilakukan dengan metode seleksi individu. Penelitian ini bertujuan untuk mengevaluasi pewarisan marka Cyca-DAB1*05 dan keragaman genotipe ikan mas varietas Rajadanu tahan infeksi KHV dan tumbuh cepat generasi ketiga (F3). Sebanyak 44 individu ikan mas Rajadanu F3 digunakan pada penelitian ini. Evaluasi marka Cyca-DAB1*05 dilakukan dengan metode PCR menggunakan primer spesifik marka Cyca-DAB1*05. Analisis keragaman genotipe dilakukan menggunakan tiga lokus mikrosatelit (MFW6, MFW7, dan MFW9). Data alel mikrosatelit dianalisis menggunakan program Fstat dan Arlequin. Hasil penelitian menunjukkan bahwa semua individu pada populasi ikan mas Rajadanu F3 membawa marka Cyca-DAB1*05. Ikan mas Rajadanu F3 memiliki keragaman genotipe yang relatif rendah. Nilai rata-rata heterozigositas teramati (0,31) lebih kecil daripada rata-rata heterozigositas harapan (0,46). Nilai positif pada indeks fiksasi (0,32) menunjukkan status inbreeding pada populasi tersebut. Hasil penelitian ini dapat menjadi acuan, baik dalam kegiatan manajemen induk maupun dalam program seleksi selanjutnya dengan tujuan untuk mempertahankan keragaman genetik dan meminimasi tekanan inbreeding ikan mas Rajadanu tahan infeksi KHV dan tumbuh cepat.Research Institute for Fish Breeding (RIFB) has created Rajadanu common carp resistant to koi herpesvirus (KHV) infection and fast growth through selection program. Rajadanu common carp has been produced by independent culling selection on resistant to KHV and fast growth characters. Selection on resistant to KHV and fast growth character was conducted using Cyca-DAB1*05 marker and mass-selection method respectively. The aim of this study was to evaluate the inheritance of Cyca-DAB1*05 as a marker and the genotype variation of Rajadanu common carp resistant to KHV infection and fast growth. A total of 44 fish were used for this study. Evaluation of Cyca-DAB1*05 marker was conducted by PCR method using specific primer to Cyca-DAB1*05 marker. Analysis of genotype variation was conducted using three microsatellite loci (MFW6, MFW7, and MFW9). Microsatellite allele data was analyzed using Fstat and Arlequin software. The result showed that all of F3 Rajadanu common carp were positive carrying Cyca-DAB1*05 marker. Genetic variation of this population was relatively low. The average observed heterozygosity (Ho=0.31) was lower than the average expected heterozygosity (He=0.46). Positive value of fixation index (0.32) showed inbreeding status in this population. The result of this study can be use as reference for broodstock management and next selection program to maintain the genetic diversity and to minimize inbreeding depression level of Rajadanu common carp resistant to KHV infection and fast growth.
PERFORMA PERTUMBUHAN KRABLET KEPITING BAKAU (Scylla olivacea) DENGAN FREKUENSI PEMBERIAN PAKAN BERBEDA PADA STADIA PENDEDERAN Kamaruddin Kamaruddin; Usman Usman; Asda Laining
Jurnal Riset Akuakultur Vol 11, No 2 (2016): (Juni 2016)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (400.235 KB) | DOI: 10.15578/jra.11.2.2016.163-170

Abstract

Salah satu upaya untuk menekan tingkat kanibalisme dan memicu pertumbuhan krablet kepiting bakau adalah dengan pemberian pakan yang cukup, baik jumlah maupun mutu. Penelitian ini bertujuan mengevaluasi kinerja pertumbuhan dan sintasan krablet kepiting bakau dengan frekuensi pemberian pakan berbeda pada stadia pendederan. Hewan uji berupa krablet kepiting bakau dengan bobot awal 0,037 ± 0,005 g dan lebar karapas awal 3,43 ± 0,42 mm; menggunakan sembilan unit fiber gelas dengan ukuran masing-masing 1 m x 1 m x 0,5 m. Hewan uji ditebar dengan kepadatan 50 ekor/m2. Perlakuan yang diujicobakan adalah frekuensi pemberian pakan per hari, yaitu: (A) dua kali (pukul 08.00 dan 18.00), (B) tiga kali (pukul 08.00, 13.00, dan 18.00), dan (C) empat kali (pukul 08.00, 13.00, 18.00, dan 23.00). Pakan yang digunakan berupa pelet dengan kandungan protein kasar 46,5%; lemak 8,8%; dan energi total 18,8 MJ/kg. Dosis pakan harian sebanyak 30%-15% bobot badan secara menurun hingga akhir penelitian. Setelah lima minggu pemeliharaan didapatkan hasil yang menunjukkan bahwa pertumbuhan, sintasan, konsumsi pakan harian, rasio konversi pakan, dan rasio efisiensi protein tidak menunjukkan perbedaan yang nyata (P>0,05) di antara perlakuan. Namun demikian, pemberian pakan empat kali sehari cenderung menunjukkan pertumbuhan yang lebih cepat dan dengan ukuran krablet yang lebih seragam.One of technique to reduce cannibalism and to stimulate the growth of mud crab is by providing an adequate feed that meet the requirement level of the crablet. The objecive of this study was to evaluate the growth and survival of crablet fed artificial diet with different feeding frequencies during nursery stage. The treatments were different feeding frequencies namely (A) 2 times (08:00 and 18:00), (B) 3 times (08:00, 13:00, and 18:00 ) and (C) 4 times (08:00, 13:00, 18:00 and 23:00). Feeding rate was from 30 to 15 % of biomass/day. Feed used in the feeding trial was a dry pellet (1.2 mm size) containing 46.5 % crude protein, 8.8 % lipid and gross energy 18.8MJ / kg. Mud crab crablet with initial body weight of 0.037 ± 0.005 g with carapace width of 3.43 ± 0.042 mm were stocked into nine fiber tanks (1 m x 1 m x 0.5 m) with density of 50 crablets / tank. After 5 weeks of feeding trial, results of the experiment showed that growth rate, survival, feed intake, feed conversion ratio and protein efficiency ratio were not significantly difference (P> 0.05) among the treatments. However, growth performance of crablet fed 4 times/day increased and resulted uniformsize of crablet.
RAGAM GENOTIPE IKAN TENGADAK, Barbonymus schwanenfeldii (Bleeker 1854) PERSILANGAN POPULASI JAWA DAN KALIMANTAN BERDASARKAN RAPD Deni Radona; Dinar Tri Soelistyowati; Rudhy Gustiano; Odang Carman; Irin Iriana Kusmini; Sri Sundari
Jurnal Riset Akuakultur Vol 11, No 2 (2016): (Juni 2016)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (166.663 KB) | DOI: 10.15578/jra.11.2.2016.99-105

Abstract

Dalam rangka pengelolaan sumber genetik untuk pengembangan budidaya ikan tengadak maka perlu dilakukan evaluasi sumber genetik ikan tengadak asal Jawa dan Kalimantan, serta progeni hibridanya. Analisis genetik dilakukan secara molekuler dengan metode RAPD. Jumlah sampel yang digunakan untuk analisis RAPD sebanyak 10 ekor setiap populasi. Spesimen yang digunakan untuk analisis RAPD adalah sirip untuk induk dan whole body untuk hibrida. Hasil menunjukkan polimorfisme (32,43%) dan heterozigositas (0,13) tertinggi terdapat pada ikan tengadak hasil persilangan betina Jawa x jantan Kalimantan, sedangkan yang terendah diperoleh pada persilangan betina Kalimantan x jantan Jawa (polimorfisme: 21,62% dan heterozigositas: 0,10). Berdasarkan dendrogram hubungan kekerabatan interpopulasi ikan tengadak hasil persilangan (betina Kalimantan x jantan Jawa) dengan induknya (populasi Jawa dan Kalimantan) menggunakan tiga primer RAPD (OPA-08, OPA-09, dan OPC-02) menunjukkan jarak genetik berkisar 0,48. Ikan tengadak betina asal Jawa dan jantan asal Kalimantan potensial meningkatkan keragaman genetik.In order to maintain the genetic sources of tinfoil barb for aquaculture development, it is necessary to evaluate the genetic diversity crossbred results of tinfoil barb from Java and Kalimantan. The genetic assessment was conducted by genotype trails using RAPD methods. The samples used for the analysis of RAPD was as much as 10 individuals. Specimens used for RAPD analysis was a fin for broodstock and whole body for the hybrid fish. The result showed that the highest polymorphism and heterozygosity were found 32.43% and 0.13 in population crossbred of female Java x male Kalimantan. While the lowest polymorphism and heterozygosity were detected on population of f Kalimantan x m  Java (21.62% and 0.10, respectively). Based on the relationship between tinfoil barb hybrid (female Kalimantan x male Java) with a both broodstock population using three RAPD primers (OPA-08, OPA-09, and OPC-02) resulted in genetic distance of 0.48. Females tinfoil barb from Kalimantan and males from Java potential could increase genetic diversity. 
KERAGAAN PERTUMBUHAN DAN WARNA IKAN WILD BETTA (Betta sp.) DENGAN REKAYASA INTENSITAS CAHAYA DAN WARNA LATAR Riani Rahmawati; Sawung Cindelaras; Eni Kusrini
Jurnal Riset Akuakultur Vol 11, No 2 (2016): (Juni 2016)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (904.184 KB) | DOI: 10.15578/jra.11.2.2016.153-162

Abstract

Penelitian ini dilakukan untuk menguji pengaruh kondisi lingkungan dengan intensitas cahaya dan warna latar yang berbeda terhadap pertumbuhan dan warna ikan cupang (Betta sp.). Biota uji diberi kombinasi perlakuan perbedaan kondisi cahaya dan warna latar, yaitu: (A-1) 100 lux dan latar putih; (A-2) 100 lux dan latar hitam; (A-3) 100 lux dan latar biru; (B-1) 800 lux dan latar putih; (B-2) 800 lux dan latar hitam; (B-3) 800 lux dan latar biru; (C-1) 1.500 lux dan latar putih; (C-2) 1.500 lux dan latar hitam; dan (C-3) 1.500 Lux dan latar biru. Setiap perlakuan diulang tiga kali. Ikan diberikan pakan berupa larva Chironomus sp. secara ad libitum. Penelitian dilakukan selama 84 hari. Hasil terbaik diperoleh pada perlakuan A-1 (intensitas 100 lux dan latar putih), dengan laju pertumbuhan spesifik panjang dan bobot masing-masing sebesar 0,48 ± 0,06%/hari dan 0,75 ± 0,22%/hari; sintasan sebesar 100%, serta persentase perubahan hue (H), saturation (S), dan brightness (B) berturut-turut adalah 21 ± 18, 6 ± 10, dan 6 ± 18.This research was conducted to determine the effect of rearing environment and light intensity with different background coloration to improve the color quality and growth of wild betta fish (Betta sp.). The fishs which were placed in the small tanks and treated with different light intensities and background coloration. The treatments combinations were: (A-1) 100 lux and white background, (A-2) 100 lux and black background, (A-3) 100 lux and blue background, (B-1) 800 lux and white background, (B-2) 800 lux and black background, (B-3) 800 lux and black background, (C-1) 1,500 lux and white background, (C-2) 1,500 lux and black background, and (C-3) 1,500 lux and blue background. Each treatment was repeated three times. The fish were fed onfrozen Chironomus larvae twice a day. The research was conducted for 84 days. The result showed that the combination of 100 lux and white background resulted the best growth rate (0.48 ± 0.06 cm/day in length and 0.75 ± 0.22%/day in weight) and survival rate of 100%. The best combination had the value of hue (21 ± 18), saturation (6 ± 10), and brightness (6 ± 18).
BARCODING DNA IKAN HIAS LAHAN GAMBUT Melta Rini Fahmi; Anjang Bangun Prasetio; Ruby Vidia Kusumah; Erma Primanita Hayuningtyas; Idil Ardi
Jurnal Riset Akuakultur Vol 11, No 2 (2016): (Juni 2016)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (553.334 KB) | DOI: 10.15578/jra.11.2.2016.137-145

Abstract

Perairan gambut merupakan ekosistem unik yang memiliki kekayaan biodiversitas ikan, sebagian besar di antaranya memiliki potensi sebagai ikan hias. Penelitian ini dilakukan untuk melakukan identifikasi dan analisis keragaman genetik, karakter genetik, jarak genetik, dan pohon kekerabatan ikan-ikan yang mendiami perairan gambut Cagar Biosfere Bukit Batu Provinsi Riau. Tahap pertama penelitian ini adalah identifikasi secara morfologi terhadap 29 ikan hasil koleksi yang potensial sebagai ikan hias. Selanjutnya amplifikasi dan alignment 675 bp (base pair) dari 90 runutan parsial cytochrome c oxidase subunit 1 (COI). Hasil penelitian menunjukkan ikan yang diidentifikasi dapat dikelompokkan menjadi enam famili, yaitu Balontidae terdiri atas tiga spesies (12,5%); Cyprinidae 13 spesies (54,17%); Cobitidae satu spesies (4,17%); Siluridae dua spesies (8,3%); Datnoidae satu spesies (4,17%); dan Bagridae empat spesies (16,67%). Beberapa spesies memiliki perbedaan genetik intraspesies lebih dari 3%. Analisis kekerabatan dan clustering ikan hias lahan gambut berdasarkan gen COI memiliki nilai bootstrap 87-99 per ulangan.Peat is a unique ecosystem which a high fish biodiversity, and most of them are potential as ornamental fish. This research was conducted to identify and analyze genetic diversity, genetic code, genetic distances, and phylogenies of the fish that inhabit in the Bukit Batu Biosfere Reserves, Riau Province. The first stage of this study was identification of 29 fish species that potential as ornamental fish by using morphological character. The further stages were amplification and alignment of 675 base pairs of 90 partial sequences of cytochrome c oxidase subunit 1 (COI). Results showed that the identification based on COI could be classified into six families. These Families were Balontidae, Cyprinidae, Cobitidae, Siluridae, Datnoidae, and Bagridae which consist of three species (12.5%), 13 species (54.17%), one species (4.17%), two species (8.3%), one species (4.17%), and four species (16.67%), respectively. Some clustered have intra-species genetic divergence more than 3%. Phylogenetic and clustering analysis showed all of the OTU (0perational Taxonomic Unit) has a high bootstrap permutation of 87-99.

Page 1 of 1 | Total Record : 10

 1 

Filter by Year

2016 2016


Reset
Filter By Issues
All Issue Vol 20, No 4 (2025): Desember (2025) Vol 20, No 3 (2025): September (2025) Vol 20, No 2 (2025): Juni (2025) Vol 20, No 1 (2025): Maret (2025) Vol 19, No 4 (2024): Desember (2024) Vol 19, No 3 (2024): September (2024) Vol 19, No 2 (2024): Juni (2024) Vol 19, No 1 (2024): (Maret 2024) Vol 18, No 4 (2023): (Desember, 2023) Vol 18, No 3 (2023): (September, 2023) Vol 18, No 2 (2023): (Juni, 2023) Vol 18, No 1 (2023): (Maret 2023) Vol 17, No 4 (2022): (Desember 2022) Vol 17, No 3 (2022): (September) 2022 Vol 17, No 2 (2022): (Juni) 2022 Vol 17, No 1 (2022): (Maret, 2022) Vol 16, No 4 (2021): (Desember, 2021) Vol 16, No 3 (2021): (September, 2021) Vol 16, No 2 (2021): (Juni, 2021) Vol 16, No 1 (2021): (Maret, 2021) Vol 15, No 4 (2020): (Desember, 2020) Vol 15, No 3 (2020): (September, 2020) Vol 15, No 2 (2020): (Juni, 2020) Vol 15, No 1 (2020): (Maret, 2020) Vol 14, No 4 (2019): (Desember, 2019) Vol 14, No 3 (2019): (September, 2019) Vol 14, No 2 (2019): (Juni, 2019) Vol 14, No 1 (2019): (Maret, 2019) Vol 13, No 4 (2018): (Desember 2018) Vol 13, No 3 (2018): (September 2018) Vol 13, No 2 (2018): (Juni, 2018) Vol 13, No 1 (2018): (Maret 2018) Vol 12, No 3 (2017): (September 2017) Vol 12, No 4 (2017): (Desember 2017) Vol 12, No 2 (2017): (Juni 2017) Vol 12, No 1 (2017): (Maret 2017) Vol 11, No 3 (2016): (September 2016) Vol 11, No 4 (2016): (Desember 2016) Vol 11, No 2 (2016): (Juni 2016) Vol 11, No 1 (2016): (Maret 2016) Vol 8, No 3 (2013): (Desember 2013) Vol 5, No 3 (2010): (Desember 2010) Vol 5, No 2 (2010): (Agustus 2010) Vol 5, No 1 (2010): (April 2010) Vol 2, No 2 (2007): (Agustus 2007) Vol 2, No 1 (2007): (April 2007) Vol 1, No 1 (2006): (April 2006) Vol 10, No 4 (2015): (Desember 2015) Vol 10, No 3 (2015): (September 2015) Vol 10, No 2 (2015): (Juni 2015) Vol 10, No 1 (2015): (Maret 2015) Vol 9, No 3 (2014): (Desember 2014) Vol 9, No 2 (2014): (Agustus 2014) Vol 9, No 1 (2014): (April 2014) Vol 8, No 2 (2013): (Agustus 2013) Vol 8, No 1 (2013): (April 2013) Vol 7, No 3 (2012): (Desember 2012) Vol 7, No 2 (2012): (Agustus 2012) Vol 7, No 1 (2012): (April 2012) Vol 6, No 3 (2011): (Desember 2011) Vol 6, No 2 (2011): (Agustus 2011) Vol 6, No 1 (2011): (April 2011) Vol 4, No 3 (2009): (Desember 2009) Vol 4, No 2 (2009): (Agustus 2009) Vol 4, No 1 (2009): (April 2009) Vol 3, No 3 (2008): (Desember 2008) Vol 3, No 2 (2008): (Agustus 2008) Vol 3, No 1 (2008): (April 2008) Vol 2, No 3 (2007): (Desember 2007) Vol 1, No 3 (2006): (Desember 2006) Vol 1, No 2 (2006): (Agustus 2006) More Issue