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INDONESIA
JURNAL SELULOSA
Published by Kementerian Perindustrian Republik Indonesia
ISSN : -     EISSN : -     DOI : -
Core Subject : Education,
Education
Jurnal Selulosa (JSel) is a journal that provides scientific information resources aimed at researchers and engineers in academia, research institutions, government agencies, and industries. Jurnal Selulosa publishes original research papers, review articles and case studies focused on cellulose, cellulose derivatives, pulp technology, paper technology, environment, biorefinery and other related topics. Formerly known as Berita Selulosa, and the first publication was in 1965. Since 2011, the journal renamed to Jurnal Selulosa.
Arjuna Subject : -
Articles 204 Documents
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PEMBUATAN MIKROKRISTALIN SELULOSA ROTAN MANAU (Calamus manan sp.) SERTA KARAKTERISASINYA ., Steven; ., Mardiyati; Suratman, R.
JURNAL SELULOSA Vol 4, No 02 (2014): JURNAL SELULOSA
Publisher : Center for Pulp and Paper

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (542.503 KB) | DOI: 10.25269/jsel.v4i02.84

Abstract

Rattan is one of natural resources of Indonesia which contains 30%-40% cellulose. Its high cellulose contents makes it very potential as a source of microcrystalline cellulose (MCC). In this research, manau rattan was characterized by using the Chesson methods. Microcrystalline cellulose was prepared by using two methods, i.e. alkalization and acid hydrolysis. Alkalization was performed by soaking manau rattan powder into sodium hydroxide 17,5% for 8 hours. Acid hydrolysis was prepared by using sulfuric acid at a concentration of 0.1 M; 0.3 M; and 0.5 M for 4, 6, 8, and 10 hours. The crystallinity of MCC was quantitatively measured by XRD and qualitatively measured by using FTIR. In this research, we have successfully made microcrystalline cellulose from manau rattan. The highest crystallinity MCC of 72.42% was obtained from acid hydrolysis with 0.5 M for 10 hours. The crystallinity of the MCC product increases with concentration and hydrolysis time.Keywords: acid hydrolisis, alkalization, cellulose, manau rattan, MCCABSTRAKRotan merupakan salah satu kekayaan hayati Indonesia yang mengisi sepuluh persen hutan di Indonesia yang memiliki kadar selulosa mencapai 30-40%. Kadar selulosa yang cukup tinggi membuat rotan sangat berpotensi untuk dimanfaatkan sebagai bahan baku mikrokristalin selulosa (MCC). Rotan manau dikarakterisasi menggunakan metode Chesson. Pembuatan mikrokristalin selulosa terdiri atas dua tahap, yaitu alkalisasi dan hidrolisi asam. Tahap alkalisasi dilakukan dengan merendam rotan di dalam larutan NaOH 17,5% selama 8 jam. Tahap hidrolisis asam dilakukan dengan menggunakan asam sulfat pada berbagai konsentrasi, yakni 0,1; 0,3 dan 0,5 M selama 4, 6, 8, dan 10 jam. Kristalinitas dari MCC yang dihasilkan diukur dengan menggunakan XRD. Untuk mengetahui komposisi kimia serta kristalinitas MCC secara kualitatif, telah dilakukan karakterisasi dengan menggunakan FTIR. Pada penelitian ini, telah berhasil dibuat MCC yang bersumber dari rotan manau. Kristalinitas MCC tertinggi dihasilkan dengan perlakuan hidrolisis asam dengan konsentrasi 0,5 M selama 10 jam, yaitu sebesar 72,42%. Seiring dengan peningkatan konsentrasi serta waktu hidrolisis, kristalinitas MCC yang dihasilkan semakin tinggi.Kata kunci: alkalisasi, hidrolisis asam, selulosa, MCC, rotan manau
POTENSI KOMPOS DARI LIMBAH PADAT PABRIK JOSS PAPER UNTUK MENINGKATKAN PRODUKTIVITAS TANAMAN (THE POTENCY OF COMPOST FROM JOSS PAPER MILL SOLID WASTE TO IMPROVE THE CROP PRODUCTION) Soetopo, Rina S.; Septiningrum, Krisna; Surahman, Aep
JURNAL SELULOSA Vol 45, No 01 (2010): BERITA SELULOSA
Publisher : Center for Pulp and Paper

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (853.776 KB)

Abstract

A research about paper sludge from joss paper industry as compost material has been conducted. Initially, paper sludge from waste water treatment plant was characterized. Cellulose degrading fungi were added as activator in composting experiment. Mature compost test using sensitive plant, tomato, was performed using paper sludge by product. Plant bioassay using Albazia was carried out using optimum dosage from mature compost test. Result showed that paper sludge from joss paper industry contain high content of organic matter thus can be use as organic compost. Composting experiment was performed using sawdust as bulking agents for ± 30 days, with 0,6% of cellulolytic fungi (weigth basis) as activator agents. Compost T-1 product quality was in complience with the Indonesian National Standard requirements. The extract compost materials (T-1) from paper sludge composting did showed positive effect to tomato plant. Meanwhile, plant bioassay showed that growth of Albazia using compost T-1 70% (10,5 kg/tree) product was better than control.Keywords : solid waste, T. harzianum,, cellulose, compost, joss paper millINTISARIPenelitian terhadap potensi limbah padat pabrik joss paper sebagai kompos telah dilakukan. Penelitian diawali dengan karakterisasi terhadap potensi limbah padat pabrik kertas yang  berasal dari Instalasi Pengolahan Air Limbah (IPAL). Proses pengomposan dilakukan dengan menambahkan aktivator jamur pendegradasi selulosa. Terhadap kompos yang telah memenuhi syarat, dilakukan uji potensi terhadap tanaman tomat sebagai tanaman sensitif. Dosis optimum kompos pada tanaman tomat, diujicobakan ke tanaman sengon. Hasil penelitian menunjukkan bahwa limbah padat IPAL pabrik joss paper memiliki bahan organik yang cukup tinggi, sehingga memiliki potensi untuk dimanfaatkan sebagai kompos organik. Pengomposan dengan  penambahan aktivator jamur selulolitik 0,6% dari berat total limbah kering dilakukan selama 30 hari menghasilkan kualitas kompos (T-1) yang memenuhi persyaratan SNI. Hasil uji kompos (T-1) terhadap tanaman tomat menunjukkan pengaruh positif pada pertumbuhan vegetatif maupun generatif. Uji coba kompos (T-1) sampai dosis 70% (10,5 kg/pohon) terhadap tanaman sengon menunjukkan pertumbuhan vegetatif yang jauh lebih baik dibanding kontrol.Kata kunci : limbah padat, T. harzianum, selulosa, kompos, pabrik joss paper
KEEFEKTIFAN BEBERAPA DEKOMPOSER UNTUK PENGOMPLOSAN LIMBAH SLUDGE PABRIK KERTAS SEBAGAI BAHAN BAKU PUPUK ORGANIK Widiastuti, Happy; ., Isroi; ., Siswanto
JURNAL SELULOSA Vol 44, No 02 (2009): BERITA SELULOSA
Publisher : Center for Pulp and Paper

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (6334.244 KB) | DOI: 10.25269/jsel.v44i02.137

Abstract

Sludge, a solid waste by-product of paper industry, is very useful as raw materials for organic fertilizer, since this material contains carbon, and others mineral such as nitrogen, phosphorus, and pottasium. This researh evaluated 128 treatments which is combination of32 decomposers and four types of media. Thirty two decomposer are combination of 5 selected isolates i.e. omphalina sp., agrayli sp., pholyota sp., T. Pseudokoningii, and unidentified fungsi isolated from basal stem of coconut, while four types of media were sludge, sludge +cocopit, sludge+manure, sludge+cocopit+manure. composting of sludge was conducted in bag log containing 0.5 kg dry weight of sludge. Optimim condition of composting of sludge was obtained by supplementing with manure in combination with the composer (Polyota sp. And mixture of omphalina sp.+agraylie sp.+ unidentfiied fungsi isolated from basal stem of coconut). This condition can enchaane the rate of composting process, showed by reducing dry weight of compost and C concentration, and the highest percentage of carbon reducing. Application of this organic fertilizer increase the growth, N and PKey words: sludge, organic, fertilizer, nutrient tissue contentINTISARI Sludge instalasi pengolahan air limbah (IPAL) sebagai limbah pabrik padat kertas memiliki kandungan karbon organik yang tinggi juga mengandung beberapa mineral, seperti nitrogen, pospor dan kalium. Dengan karakteristik ini maka limbah padat mempunyai potensi sebagai bahan baku pupuk organik. Dalam penelitian ini diuji 128 perlakun yng merupaka kombinasi 32 jenis dekomposer terseleksi dan 4 jenis medium. 32 dekomposer yang diuji merupakan kombinasi dari 5 isolat terseleksi yaitu Omphalina sp., agrayly sp., pholyota sp., T. Pseudokoningii, dan isolat dari pangkal batang kelapa sedangkan empat jenis medium yang diuji merupakan limbak kandang, pengomposan dilakukan di bag log yang diisi0,5 kg medium. Kondisi optimum pengomposan limbah padat yang diperoleh ialah penambahan pupuk kandang yang dikombinasi dengan dekomposer yang diamati dari besarnya penurunan bobot kering kompos, persentase kadat C serta persentase penurunan kandungan CKata kunci: sludge, pupuk organik, kandungan hara jaringan
POTENSI PENGGUNAAN ABU BOILER INDUSTRI PULP DAN KERTAS SEBAGAI BAHAN PENGKONDISI TANAH GAMBUT PADA AREAL HUTAN TANAMAN INDUSTRI Purwati, Sri; Soetopo, Rina; Setiawan, Yusup
JURNAL SELULOSA Vol 42, No 01 (2007): BERITA SELULOSA
Publisher : Center for Pulp and Paper

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (211.934 KB) | DOI: 10.25269/jsel.v42i1.180

Abstract

The utilization of boiler ash from bark burning in pulp and paper industry as a conditioning agent of peat gambut soil inindustrial plantation forest has been carried out. The utilization of boiler ash as much as 5 – 10 kg/plant on peat soil,  as a vegetation media of Acacia crasicarpa conducted in the green house and the field of timber estate. Its influences on productivity and environmental aspect has been observed for 12 months vegetation age. The results showed that there were a significant effect of boiler ash utilization on growth rate and productivity of plantations. Utilization up to 10 kg/plant or 10 tons/ha/plantation period was improving the physical, chemical, and biological properties of boiler soil without detrimental effects to the environment.
PENANGANAN STICKY DAN PITCH SECARA ENZIMATIS PADA DAUR ULANG KERTAS BEKAS Elyani, Nina; R., Jenni; Wirawan, Sonny K.; ,, Cucu
JURNAL SELULOSA Vol 1, No 01 (2011): JURNAL SELULOSA
Publisher : Center for Pulp and Paper

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (391.76 KB) | DOI: 10.25269/jsel.v1i01.16

Abstract

Sticky and pitch are constraint in the recycling process of coated print paper often occured. Research of sticky and pitch handling has been done for coated paper recycling process by applying lipase at 0.1 and 0.5 %, pH 6-7 and the temperature of 70 – 80°C for 30 minutes. The addition of conventional surfactant at 0.1 and 0.5 % by weight of dry pulp is used as comparison. The results showed that the handling of sticky and pitch enzymatically produces paper with brightness, opacity, dirt contain and tensile index better than that of using the conventional surfactant.Key words : waste paper, sticky, pitch, lipase, surfactants  ABSTRAKSticky dan pitch pada proses daur ulang kertas cetak salut merupakan kendala yang sering terjadi karena mengganggu peralatan pembuatan kertas. Pada penelitian penanganan sticky dan pitch ini telah dilakukan pada proses daur ulang kertas cetak salut bekas dengan menggunakan lipase sebesar 0,1 - 0,5 %, pH 6 – 7 dan suhu sekitar 70 - 80°C selama 30 menit. Sebagai pembanding juga dilakukan penambahan surfaktan sebesar 0,1% - 0,5% terhadap berat kering pulp. Hasil penelitian menunjukkan bahwa penanganan sticky dan pitch secara enzimatis menghasilkan derajat putih, opasitas, noda dan indeks tarik yang lebih baik dibandingkan secara konvensional yang menggunakan surfaktan. Kata kunci : kertas bekas, sticky, pitch, lipase surfaktan
AUDIT ENERGI DI MESIN KERTAS Dina, Sari Farah; Susanto, Herri
JURNAL SELULOSA Vol 41, No 02 (2006): BERITA SELULOSA
Publisher : Center for Pulp and Paper

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (311.913 KB)

Abstract

Energy audit were conducted in five paper mills, namely: A B,C, D and E with different paper machine and process conditions. Mill A is relatively old  and small with a capacity of 160 ton/day of print paper. The steam consumption of mill A was 2.01 ton/ton product, which was higher than that of mill B (1.41 ton/ton). This large different steam consumption might be due to a high water content of paper entering the paper machine (up to 62.83%). A highest steam consumption up to 3.76 ton/ton product was found in Mill E having a small capacity of 38 ton/day. Besides the fact that paper machine in Mill E was old and had no hood, type of product (grammage of 284 g/m2) might also contribute to this high steam consumption. Two other mills producing corrugated paper (mills C and D: with a grammage of 115 and 125 g/m2 respectively) had steam consumptions of about 1.9 ton/ton product, within the range of 1.8 – 2.2 ton/ton reported in literature.Mill B with a capacity of 984 ton/day was the largest mill in this study. Current steam consumption of mill B of about 1.41 ton/ton of product was the lowest among the other four mills. This mill has implemented an energy conservation program. By means of reducing water content in paper entering the drying machine from 54% to 51%, the steam consumption was reduced from 1611 to 1440 ton/day. From a simple mass and heat balance, increasing water content in paper leaving paper machine from the present value of 4% to the allowable one of 5% might also give another saving of steam.  INTISARIAudit energi telah dilakukan pada lima pabrik kertas, dinamai A, B, C, D dan E, dengan berbagai kapasitas dan kondisi proses. Pabrik A memiliki kapasitas kecil, hanya 180 ton/hari dan sudah tua. Konsumsi steam pabrik ini sekitar 2,01 ton/ton kertas, jauh lebih tinggi daripada pabrik B (1,41 ton/ton). Perbedaan mencolok ini salah satunya disebabkan perbedaan kadar air dalam kertas saat masuk mesin kertas (kadar air sampai 62,83% di pabrik A). Konsumsi steam terbesar ditemui di pabrik E (3,76 ton/ton) yang memiliki kapasitas produksi kertas 38 ton/hari. Pabrik E yang sudah tua ini memproduksi kertas tebal (gramatur 284 g/m2) dan mesin kertasnya tidak dilengkapi dengan hood. Dua pabrik kertas lainnya memproduksi corrugated-paper: pabrik C dengan gramatur 115 dan pebrik D 125 g/m2. Konsumsi steam kedua pabrik ini sekitar 1,9 ton/jam, masih dalam rentang konsumsi steam 1,8 – 2,2 ton/ton kertas yang dilaporkan di beberapa pustaka. Pabrik B merupakan pabrik terbesar dengan kapasitas 984 ton/hari, telah menerapkan program-program penghematan energi. Konsumsi steam pabrik ini adalah 1,41 ton/ton produk, paling rendah di antara kelima pabrik yang ditinjau. Dengan menurunkan kadar air pada kertas sebelum masuk mesin pengering, pabrik ini dapat menghemat pemakaian steam dari 1611 menjadi 1440 ton/hari. Disamping itu perhitungan neraca massa dan energi menunjukkan masih adanya penghematan konsumsi steam jika kadar air dalam kertas keluar mesin pengering dapat dinaikkan dari kebiasaan 4% menjadi yang diijinkan 5%.
CELLULOSIC MATERIALS AS POLYMER ELECTROLYTE MEMBRANE IN FUEL CELL APPLICATION Radiman, Cynthia L.; Sarinastiti, A.
JURNAL SELULOSA Vol 2, No 02 (2012): JURNAL SELULOSA
Publisher : Center for Pulp and Paper

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (793.513 KB) | DOI: 10.25269/jsel.v2i02.32

Abstract

Polymer Electrolyte Membrane Fuel Cells (PEMFC) and Direct Methanol Fuel Cell (DMFC) are considered as future power sources in overcoming the fossil-based energy crisis. The objective of this work is to explore one of the natural resources of Indonesia and to improve its properties by chemical modification in order to get the required characteristics as electrolyte membrane. In this work coconut water was used as the basic material. It was fermented by Acetobacter xylinum and the resulting bacterial cellulose was then phosphorylated using a microwave-assisted reaction. Those membranes have been immersed for a varied time ranging between 0 and 8 hours (0-8 h) in a mixture of N,N-dimethylformamide (DMF), phosphoric acid and urea prior to irradiation by microwave for 60 s. Those membranes were characterized by several methods, such as functional group analysis by FTIR, proton conductivity, ion exchange capacity, swelling index, morphology analysis by SEM and phosphorus content analysis by SEM-EDS. From the experimental data, it can be concluded that a phosphorylated bacterial cellulose prepared by a 4h–immersion could be used as an alternative electrolyte membrane for fuel cell applications.Keywords: fuel cell, bacterial cellulose, phosphorylated bacterial cellulose  ABSTRAK Sel bahan bakar membran polimer eletrolit (PEMFC) dan sel bahan bakar metanol (DMFC) merupakan sumber energi masa depan yang dapat mengatasi krisis energi minyak bumi. Tujuan penelitian ini adalah untuk menggunakan bahan alam Indonesia yang dimodifikasi secara kimiawi supaya sifat-sifatnya dapat ditingkatkan untuk memenuhi persyaratan sebagai membran elektrolit. Dalam penelitian ini air kelapa telah digunakan sebagai bahan dasar dan selanjutnya difermentasi oleh Acetobacter xylinum. Selulosa bakterial yang dihasilkan difosforilasi dengan bantuan gelombang mikro. Membran tersebut direndam selama waktu yang bervariasi ( 0-8 jam) dalam campuran N,N-dimetilasetamida (DMF), asam fosfat dan urea sebelum diiradiasi dengan gelombang mikro selama 60 detik. Karakterisasi membran dilakukan dengan berbagai metoda, antara lain analisis gugus fungsi dengan FTIR, konduktivitas proton, kapasitas penukar ion, indeks penggembungan, analisis morfologi dengan SEM dan analisis kadar fosfor dengan SEM-EDS. Dari data yang diperoleh disimpulkan bahwa selulosa bakterial yang difosforilasi dengan proses perendaman selama 4 jam dapat digunakan sebagai alternatif untuk membran elektrolit dalam aplikasi sel bahan bakar.Kata kunci: sel bahan bakar, selulosa bakterial, selulosa bakterial terfosforilasi
BIODEINKING SORTED WHITE LEDGER (SWL) MENGGUNAKAN SELULASE (BIODEINKING SORTED WHITE LEDGER (SWL) BY CELLULASE) Purwita, Chandra Apriana; Wirawan, Sonny Kurnia
JURNAL SELULOSA Vol 7, No 02 (2017): JURNAL SELULOSA
Publisher : Center for Pulp and Paper

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (402.858 KB) | DOI: 10.25269/jsel.v7i02.176

Abstract

Biodeinking is a process of separating ink on the fiber surface by enzymes. This research was conducted to study and optimize biodeinking condition of sorted white ledger (SWL) by cellulase. Commercial cellulase (Sigma Aldrich, Singapore) at a dose of 0.25; 0.5; 0.75; 1; 2 U/g dry weight pulp was applied to the deinking of SWL and compared with blank. The deinked pulp was then analyzed for the fiber morphology and made laboratory hand sheets for the characterization of physical and optical properties. Based on the experiment, at the optimum dose of cellulase 0.75 IU/g dry weight pulp was obtained a maximum increasement of ISO brightness by 4.7 points and a decreasement of ERIC by 34.45%. The use of celulase on the biodeinking was not affecting the fibers length and diameter so the tensile and tear index of deinked pulp unchanged. Loss of fines by enzymatic degradation causes the tear index decreased by 8.70 % and porosity increased. The increasement of coarseness resulted in the decreasement of pulp opacity.ABSTRAKBiodeinking merupakan proses pemisahan tinta pada permukaan serat menggunakan enzim. Penelitian ini dilakukan untuk mempelajari dan memperoleh kondisi optimum biodeinking sorted white ledger (SWL) menggunakan selulase. Selulase komersial (Sigma Aldrich, Singapura) dengan dosis 0,25; 0,5; 0,75; 1; 2 U/g kering pulp diaplikasikan pada deinking SWL dan dibandingkan terhadap blanko. Pulp deinked yang diperoleh kemudian dilakukan analisis morfologi serat dan dibuat lembaran tangan laboratorium untuk karakterisasi sifat fisik dan optik. Berdasarkan percobaan, pada dosis optimum selulase 0,75 IU/g kering pulp diperoleh kenaikan derajat cerah ISO maksimum sebesar 4,7 poin dan penurunan ERIC 34,45%. Analisis morfologi serat menunjukkan penggunaan selulase pada proses biodeinking tidak berpengaruh terhadap panjang dan diameter serat sehingga indeks tarik dan retak pulp deinked tidak berubah. Kehilangan fines karena degradasi enzimatik menyebabkan indeks sobek turun sebesar 8,70% dan porositas naik. Naiknya nilai coarseness menyebabkan meningkatnya opasitas pulp deinked.
KAJIAN AWAL PEMANFAATAN PULP DARI LIMBAH KEMASAN ASEPTIK UNTUK PEMBUATAN SELULOSA ASETAT Khadafi, Mahammad; Kencana, Yuniarti P.
JURNAL SELULOSA Vol 3, No 02 (2013): JURNAL SELULOSA
Publisher : Center for Pulp and Paper

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (672.642 KB) | DOI: 10.25269/jsel.v3i02.48

Abstract

The use of aseptic packaging in the world is still increasing from year to year, this causes a new matter like midden. Recycling the aseptic packaging is one of the efforts to utilize this waste. The raw material used for cellulose acetate crystal can be obtained from recycling process of aseptic packaging waste. This can be possible because pulp from aseptic packaging contain 72% needle unbleached virgin pulp. The purpose of this reasearch is to diversify the use of aseptic packaging waste by improving the technology process of acetylation for making cellulose acetate crystal. Aseptic packaging pulp was tested for the parameters such as water content, ash content, holocellulose content, α-cellulose content, lignin content, and hemicellulose content. This tested was used to know the eligibility of pulp for making cellulose acetate. The pulp was soaked with water and glacial acetic acid for swelling and conditioning. The acetylation process was done with adding glacial acetic acid and acetic acid anhydride in certain composition. Based on ASTM D 871-96 testing method, we obtained the optimum condition of acetyl content is 36.85% by adding 2.25 mL water and 35 mL acetic acid anhydride, whereas with the addition of 2.75 mL water and 30 mL acetic acid anhydride 28.28% acetyl content were obtained.Keywords : aseptic packaging pulp, acetate cellulose, acetylation process, acetyl content  ABSTRAKPenggunaan kemasan aseptik yang meningkat dari tahun ke tahun, menimbulkan masalah baru berupa limbah. Salah satu upaya pemanfaatan limbah adalah melalui proses daur ulang. Hasil proses daur ulang ini diantaranya dapat dijadikan substitusi bahan baku produk derivat selulosa berupa selulosa asetat, karena limbah kemasan aseptik mengandung pulp virgin serat panjang 72%. Tujuan dari penelitian ini adalah untuk diversifikasi penggunaan dan pemanfaatan limbah kemasan aseptik melalui proses daur ulang dan penguasaan teknologi proses asetilasi untuk produk selulosa asetat. Pulp kemasan aseptik diuji dengan parameter kadar air, kadar abu, kadar holoselulosa, kadar α selulosa, kadar lignin, dan kadar hemiselulosa untuk mengetahui apakah pulp kemasan aseptik memenuhi persyaratan untuk dibuat selulosa asetat. Perendaman pulp dilakukan dengan air dan asam asetat glasial, kemudian diperas untuk mengkondisikan pulp sebelum proses asetilasi. Proses asetilasi dilakukan dengan menambahkan asam asetat glasial dan asam asetat anhidrida dalam jumlah tertentu. Berdasarkan metode ASTMD 871-96 diperoleh kadar asetil optimal dari kristal selulosa asetat sebesar 36,85% dengan penambahan air 2,25 mL dan asetat anhydrida 35 ml, sedangkan untuk penambahan asam asetat anhidrida 30 mL dan air 2,75 mL diperoleh kadar asetil 28,28%.Kata kunci : pulp kemasan aseptik, selulosa asetat, asetilasi, kadar asetil
OPTIMASI MEDIA PRODUKSI ENZIM XILANASE DARI Bacillus sp. (Medium Optimization of Xylanase Production from Bacillus sp.) Erika, Erika; Agustrina, Rochmah; Sumardi, Sumardi; Mulyono, Mulyono
JURNAL SELULOSA Vol 6, No 01 (2016): JURNAL SELULOSA
Publisher : Center for Pulp and Paper

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (538.035 KB) | DOI: 10.25269/jsel.v6i01.72

Abstract

Xylan is a carbon source in growth medium of extracellular xylanase producing bacteria. The purpose of this study was to get the optimum medium for the growth of Bacillus sp. in producing the xylanase. The factors consist of production time, carbon, and nitrogen source, as well as simple sugars. Addition carbon source used was delignified sugarcane bagasse, rice hulls, and corn cobs with different concentrations (0.25%; 0.5%; 0.75%; and 1% w/v) . Ammonium chloride, ammonium sulfate, and sodium nitrate with different concentrations (0.08%; 0.17%; 0.26%; and 0.35% w/v) were used as a source of nitrogen, while the simple sugar used was glucose, lactose, sucrose, and xylose. The results showed that the optimum culture media of Bacillus sp. to produce xylanase is media with 0.25% natural starch from the corn cob xylan as a carbon source, 0.26% ammonium chloride as a source of nitrogen, 0.0625 grams of sugar xylose, at pH 6, incubation temperature of 40°C, and 12 hours production time. In that media, xylanase activity was 0.2 U/mL.Keywords: agricultural waste, medium optimization, xylanase, Bacillus sp.   ABSTRAKXilan merupakan sumber karbon pada media pertumbuhan bakteri penghasil enzim ekstraseluler xilanase. Tujuan penelitian ini adalah mendapatkan media optimum untuk pertumbuhan Bacillus sp. dalam memproduksi xilanase. Perlakuan percobaan terdiri dari waktu produksi, sumber karbon, sumber nitrogen, dan penambahan gula sederhana. Sumber karbon yang digunakan adalah bagas tebu, sekam padi, dan tongkol jagung dengan variasi konsentrasi 0,25%; 0,5%; 0,75%; dan 1% (b/v) . Amonium klorida, amonium sulfat, dan natrium nitrat dengan variasi konsentrasi 0,08%; 0,17%; 0,26%; dan 0,35% (b/v) digunakan sebagai sumber nitrogen, sedangkan gula sederhana yang digunakan adalah glukosa, laktosa, sukrosa, dan xilosa masing-masing sebanyak 0,0625 b/v. Hasil percobaan menunjukkan bahwa media optimum pertumbuhan Bacillus sp. untuk produksi xilanase adalah media dengan 0,25% tepung xilan dari tongkol jagung sebagai sumber karbon, 0,26% amonium klorida sebagai sumber nitrogen, 0,0625 gram gula xilosa, pada pH media 6, suhu inkubasi 40°C, serta waktu produksi 12 jam. Dalam media tersebut, aktivitas xilanase yang dihasilkan sebesar 0,2 U/mL.Kata kunci : limbah pertanian, optimasi media, xilanase, Bacillus sp. 

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