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AL-HAYAT: Journal of Biology and Applied Biology
ISSN : 26543702     EISSN : 26220725     DOI : -
Core Subject : Science,
AL-HAYAT: Journal of Biology and Applied Biology an international journal focuses on biological science, biotechnology, bioremidiation, environmental biology, and biological education. It is intended to communicate original research and article review in current issues on the subject. The subject covers field studies and literary with various perspectives, both interdiscipline and multidiscipline, i.e. ecology, technology, education and much more. This journal is published by Fakultas Sains dan Teknologi, Universitas Islam Negeri (UIN) Walisongo Semarang Indonesia, and managed to be issued twice in every volume. We warmly welcome contributions from scholars and researchers of many disciplines.
Arjuna Subject : -
Articles 257 Documents
A Review of Termite for Sustainable Green Building Kusumawardhani, Dina Tiara; Almulqu, Aah Ahmad
Al-Hayat: Journal of Biology and Applied Biology Vol. 7 No. 1 (2024)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v7i1.20704

Abstract

Although 3106 termite species have been documented worldwide, little information is available on those in Indonesia. In this review, the Google Scholar search platform was used to obtain information on termite species. From the 34 reviewed sources, they were identified in 4 provinces across Indonesia. Among these sites, we found 106 termite species from the islands of Sumatera, Kalimantan, Sulawesi, and Java. The most predominant species in all regions were Macrotermes sp, Microtermes sp, and Coptotermes sp.
Detection of NFKB1 Gene in Patients with Type 2 Diabetes Mellitus and Non Diabetics Using Polymerase Chain Reaction Method Cahyaningtyas, Priska Putri; Sayekti, Fitria Diniah Janah
Al-Hayat: Journal of Biology and Applied Biology Vol. 7 No. 2 (2024)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v7i2.20856

Abstract

A metabolic condition called type 2 diabetes mellitus is characterized by elevated blood sugar levels because of reduced insulin release by β pancreatic cells. The primary form of NF-kB is Nuclear Factor Kappa Beta subunit-1 (NFKB1), which is a gene that encodes the DNA binding protein (p50). The NFKB1 gene contributes to the oxidative stress and mild inflammatory processes that might exacerbate diabetes. The enzymatic procedure known as Polymerase Chain Reaction (PCR), multiplies a nucleotide sequence in order to identify if type 2 diabetes mellitus or non-diabetic has the NFKB1 gene. The purpose of this study was to detect the presence of the NFKB1 gene in type 2 diabetes mellitus and non-diabetes. The study used a descriptive research method using a purposive sampling technique conducted at the molecular biology laboratory of the Sekolah Tinggi Ilmu Kesehatan Nasional. The respondents in this study were 9 patients with type 2 diabetes mellitus who were prolanes of Puskesmas Wonosari I Klaten and 9 non-diabetic patients in PKK Kadilangu RT 2/RW 1, Baki, Sukoharjo. From the results of the study, it can be seen that the NFKB1 gene was detected after electrophoresis and visualized at 176 bp (base pair). The qualitative presence of the NFKB1 gene in DNA is still detectable, but the level of gene expression in the soil of transcription and translation is not yet known.
Detection of NFKB1 Gene in Patients with Type 2 Diabetes Mellitus and Non Diabetics Using Polymerase Chain Reaction Method Cahyaningtyas, Priska Putri; Sayekti, Fitria Diniah Janah
Al-Hayat: Journal of Biology and Applied Biology Vol. 7 No. 2 (2024)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v7i2.20856

Abstract

A metabolic condition called type 2 diabetes mellitus is characterized by elevated blood sugar levels because of reduced insulin release by β pancreatic cells. The primary form of NF-kB is Nuclear Factor Kappa Beta subunit-1 (NFKB1), which is a gene that encodes the DNA binding protein (p50). The NFKB1 gene contributes to the oxidative stress and mild inflammatory processes that might exacerbate diabetes. The enzymatic procedure known as Polymerase Chain Reaction (PCR), multiplies a nucleotide sequence in order to identify if type 2 diabetes mellitus or non-diabetic has the NFKB1 gene. The purpose of this study was to detect the presence of the NFKB1 gene in type 2 diabetes mellitus and non-diabetes. The study used a descriptive research method using a purposive sampling technique conducted at the molecular biology laboratory of the Sekolah Tinggi Ilmu Kesehatan Nasional. The respondents in this study were 9 patients with type 2 diabetes mellitus who were prolanes of Puskesmas Wonosari I Klaten and 9 non-diabetic patients in PKK Kadilangu RT 2/RW 1, Baki, Sukoharjo. From the results of the study, it can be seen that the NFKB1 gene was detected after electrophoresis and visualized at 176 bp (base pair). The qualitative presence of the NFKB1 gene in DNA is still detectable, but the level of gene expression in the soil of transcription and translation is not yet known.
Dimensional Stability Test of Mahogany Wood by Heat Treatment Kusumawardhani, Dina Tiara
Al-Hayat: Journal of Biology and Applied Biology Vol. 7 No. 2 (2024)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v7i2.22225

Abstract

Mahogany wood (Swietenia macrophylla) is one of Indonesia's fast-growing commercial tree species. Its wood is widely used for construction because of its great potential in the timber industry. Mahogany wood has low dimensional stability and high hygroscopic properties. This study aims to improve the quality of mahogany wood in terms of dimensional stability with the heat treatment method. Samples of mahogany wood were heat treated with three-time levels, namely control, 3 hours, and 6 hours at 180 °C. The results showed that heat treatment at 180 °C for 6 hours had the best results for maintaining wood dimensions gacorqq with a low increase in water content, increased specific gravity, low absorption ability, minor swelling of wood in three directions of wood, and the value of ASE is also common than 3 hours of treatment.
Dimensional Stability Test of Mahogany Wood by Heat Treatment Kusumawardhani, Dina Tiara
Al-Hayat: Journal of Biology and Applied Biology Vol. 7 No. 2 (2024)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v7i2.22225

Abstract

Mahogany wood (Swietenia macrophylla) is one of Indonesia's fast-growing commercial tree species. Its wood is widely used for construction because of its great potential in the timber industry. Mahogany wood has low dimensional stability and high hygroscopic properties. This study aims to improve the quality of mahogany wood in terms of dimensional stability with the heat treatment method. Samples of mahogany wood were heat treated with three-time levels, namely control, 3 hours, and 6 hours at 180 °C. The results showed that heat treatment at 180 °C for 6 hours had the best results for maintaining wood dimensions with a low increase in water content, increased specific gravity, low absorption ability, minor swelling of wood in three directions of wood, and the value of ASE is also common than 3 hours of treatment.
Differences in the Number and Karyotypes of Chromosomes of the Anura Order in Indonesia Khairati, Ikmanisa; Razak, Abdul
Al-Hayat: Journal of Biology and Applied Biology Vol. 7 No. 2 (2024)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v7i2.23808

Abstract

The diversity of an organism can be studied down to the cellular level. Cytological data related to certain organisms obtained can be used as information to classify these organisms. One of them is by examining the number and karyotypes owned by organisms. A karyotype is a chromosomal phenotype that includes its structural description. The purpose of this study is to see the difference in the number and karyotype of chromosomes in species belonging to the order Anura in Indonesia. The method used is a literature study of various studies related to chromosomes of species belonging to the order Anura. Based on the results of the analysis, there are differences in the number and karyotype of several species classified in the order Anura. The species of the order Anura studied in this study om88 are Rana rufipes, Rana parvaccola, Huia sumatrana, Fejervarya limnocharis, Limnonectes cf. grunniens, Limnonectes cf. modestus, Fejervarya cancrivora, Limnonectes blythii, Polypedates celebensis, Polypedates leucomystax, Microhyla pulchra, which belongs to the families Ranidae, Dicroglossidae, Rhacophoridae, and Microhylidae. The variation in the number of diploid chromosomes of the organism studied was in the range of 22-26 chromosomes. In addition to the number of chromosomes, there are also differences in chromosomal karyotypes in each organism
Differences in the Number and Karyotypes of Chromosomes of the Anura Order in Indonesia Khairati, Ikmanisa; Razak, Abdul
Al-Hayat: Journal of Biology and Applied Biology Vol. 7 No. 2 (2024)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v7i2.23808

Abstract

The diversity of an organism can be studied down to the cellular level. Cytological data related to certain organisms obtained can be used as information to classify these organisms. One of them is by examining the number and karyotypes owned by organisms. A karyotype is a chromosomal phenotype that includes its structural description. The purpose of this study is to see the difference in the number and karyotype of chromosomes in species belonging to the order Anura in Indonesia. The method used is a literature study of various studies related to chromosomes of species belonging to the order Anura. Based on the results of the analysis, there are differences in the number and karyotype of several species classified in the order Anura. The species of the order Anura studied in this study are Rana rufipes, Rana parvaccola, Huia sumatrana, Fejervarya limnocharis, Limnonectes cf. grunniens, Limnonectes cf. modestus, Fejervarya cancrivora, Limnonectes blythii, Polypedates celebensis, Polypedates leucomystax, Microhyla pulchra, which belongs to the families Ranidae, Dicroglossidae, Rhacophoridae, and Microhylidae. The variation in the number of diploid chromosomes of the organism studied was in the range of 22-26 chromosomes. In addition to the number of chromosomes, there are also differences in chromosomal karyotypes in each organism
In Silico Approach of DNA Barcoding in Cattleya Orchid using nrDNA and matK Markers Wiharyanti, Risma; Mercuriani, Ixora Sartika; Yulianti, Evy; Kuswandi, Paramita Cahyaningrum
Al-Hayat: Journal of Biology and Applied Biology Vol. 8 No. 1 (2025)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v8i1.25671

Abstract

The Cattleya genus is one of the most commercially valuable orchids. Identifying these orchids based on morphological and phenotypic traits has been challenging. Accurate identification is crucial for distinguishing between native and hybrid species and determining the protected status of certain Cattleya orchids. This research explored potential markers using nrDNA and matK locus for DNA barcoding of the Cattleya genus through an in silico approach. All sequences were collected from the NCBI and analyzed using ClustalX2 for alignment, BioEdit for conversion, and MEGA11 for phylogenetic tree construction. The results revealed that nrDNA showed higher genetic variation compared to matK. However, neither phylogenetic tree could discriminate species precisely, as some Cattleya species were positioned closer to the outgroup. These findings can be used as a reference to support identification of Cattleya using molecular marker. Keywords: Cattleya, matK, molecular barcode, nrDNA
In Silico Approach of DNA Barcoding in Cattleya Orchid using nrDNA and matK Markers Wiharyanti, Risma; Mercuriani, Ixora Sartika; Yulianti, Evy; Kuswandi, Paramita Cahyaningrum
Al-Hayat: Journal of Biology and Applied Biology Vol. 8 No. 1 (2025)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v8i1.25671

Abstract

The Cattleya genus is one of the most commercially valuable orchids. Identifying these orchids based on morphological and phenotypic traits has been challenging. Accurate identification is crucial for distinguishing between native and hybrid species and determining the protected status of certain Cattleya orchids. This research explored potential markers using nrDNA and matK locus for DNA barcoding of the Cattleya genus through an in silico approach. All sequences were collected from the NCBI and analyzed using ClustalX2 for alignment, BioEdit for conversion, and MEGA11 for phylogenetic tree construction. The results revealed that nrDNA showed higher genetic variation compared to matK. However, neither phylogenetic tree could discriminate species precisely, as some Cattleya species were positioned closer to the outgroup. These findings can be used as a reference to support identification of Cattleya using molecular marker. Keywords: Cattleya, matK, molecular barcode, nrDNA
Antioxidant Content and Vitamin C of Combination Tea of Rose Flower and Katuk Leaf based on Drying Temperature Variation Viscasari, Berliana; Ambarwati
Al-Hayat: Journal of Biology and Applied Biology Vol. 8 No. 1 (2025)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v8i1.25685

Abstract

Rose flowers and katuk leaves have not been maximally utilized. people only use rose flowers as ornamental plants and katuk leaves as vegetables. Though many bioactive compounds contained in both plants such as antioxidants and vitamin C. This study aims to examine the effect of drying temperature on the physical and chemical properties of tea combined with rose flowers and katuk leaves. the research method used a factorial complete randomized design (CRD) with a variation of drying temperatures of 50°C, 60°C, and 70°C for 5 hours, each repeated 3 times. the research parameters included chemical analysis, namely antioxidant levels (DPPH method), and vitamin C (iodimetry titration), as well as analysis of physical properties, namely pH value (pH meter), and evaluation of color, taste, and aroma. The results showed that drying temperature had a significant effect on antioxidant levels, vitamin C, pH value, color, and taste of tea, but had no effect on aroma. Drying temperature of 50°C produced the best quality with antioxidant content of 49.60%, vitamin C of 85.05 mg/100g, pH value of 6.56, light brown color, slightly bitter taste, and distinctive aroma of katuk leaves. In conclusion, a drying temperature of 50°C is recommended to produce rose flower and katuk leaf combination tea with the best physical and chemical quality.