Garuda - Garba Rujukan Digital

Authentication of Wax Apple (Syzygium samarangense (Blume) Merr. & L.M Perry) Delima and Citra Cultivars by Morphological and Molecular Approach Mukaromah, Arnia Sari; Ulfah, Malia; Rachmah, Annisa Nur; Arfan, Muhammad Ramdhani; Kusumarini, Niken; Febriana, Asri
Al-Hayat: Journal of Biology and Applied Biology Vol. 6 No. 1 (2023)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v6i1.18713

Wax apple Delima and Citra Cultivars are two superior non-climacteric tropical fruit commodities from Demak Regency which have similar morphological characters but have different fruit characteristics. Identification wax apple cultivars from Demak regency using DNA barcoding approach has not been researched yet. The aims of this research are to analyze the morphological similarities of wax apple between Delima and Citra cultivars and to identify genetic variations of wax apple Delima and Citra using trnL-trnF intergenic spacer for molecularly authentication. The results shown that there were no differences in the environmental parameters of the wax apple two cultivated area in Demak Regency. According to the morphological approach, the Delima and Citra cultivars in Demak Regency were grouped separately into Delima and Citra clusters with a similarity index of 61.5% (Citra cultivars) and 60.5% (Delima cultivars) and separated based on the origin of their cultivation area. Genetic variations between wax apple Delima and Citra in Demak Regency consisted of deletions (9delA & 17delA) and conserved P6 loop in all compared cultivars. The possibility of heteroplasmy R (A,G) found at 73 nucleotide number in Delima Betokan cultivar and Citra cultivars (Betokan and Jungpasir). Meanwhile, Delima Jungpasir cultivar is K (G,T). There were P8 stem-loops with different lengths between Delima cultivars (Betokan and Jungpasir) and the same length in Citra cultivars (Betokan and Jungpasir). Therefore, the trnL-trnF intergenic spacer has not been thoroughly used in wax apple Delima and Citra authentication due to the presence of a secondary structure which causes the loss of the electropherogram signal so that the nucleotide sequence cannot be read.

Deteksi Bakteri Endofit Dan Pengaruh Microbacterium arborescens Terhadap Pertumbuhan Sorgum Manis (Sorghum bicolor) Putri, Riana Nindita
Al-Hayat: Journal of Biology and Applied Biology Vol. 4 No. 2 (2021)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v4i2.18820

This research aims to detect the presence of potential endophytic bacteria, one of which is Microbacterium arborescens and study the effects on the growth of local variants of sweet sorghum plants from farmers in Sleman, Yogyakarta. This research began with infection of Microbacterium arborescens bacteria on sorghum seeds. Sorghum plants were grown in sterile media for 14 days after germination to observe seven growth parameters, including root length, number of lateral roots, stem length, number of leaves, leaf length, wet weight, and dry weight. Analysis of sweet sorghum growth parameters based on the one way ANOVA statistical test. Samples of infected and uninfected sorghum plants in the roots, stems, and leaves were amplified for the 16s rRNA gene using Bioline PCR hotstart mix. The primers used are universal primer Primer 27 f (59-AGA GTT TGA TCM TGG CTC AG-39) and 1492 r (59- TAC GGH TAC CTT GTT ACG ACT T-39). The root length and number of lateral roots between sweet sorghum with and without Microbacterium arborescens infection showed significant differences. The length of the control roots was 9.24 cm, the treatment plants were 6.38 cm, the control plants had six treatment roots, and the treatment plants had ten lateral roots.

Deteksi Bakteri Endofit Dan Pengaruh Microbacterium arborescens Terhadap Pertumbuhan Sorgum Manis (Sorghum bicolor) Putri, Riana Nindita
Al-Hayat: Journal of Biology and Applied Biology Vol. 4 No. 2 (2021)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v4i2.18820

This research aims to detect the presence of potential endophytic bacteria, one of which is Microbacterium arborescens and study the effects on the growth of local variants of sweet sorghum plants from farmers in Sleman, Yogyakarta. This research began with infection of Microbacterium arborescens bacteria on sorghum seeds. Sorghum plants were grown in sterile media for 14 days after germination to observe seven growth parameters, including root length, number of lateral roots, stem length, number of leaves, leaf length, wet weight, and dry weight. Analysis of sweet sorghum growth parameters based on the one way ANOVA statistical test. Samples of infected and uninfected sorghum plants in the roots, stems, and leaves were amplified for the 16s rRNA gene using Bioline PCR hotstart mix. The primers used are universal primer Primer 27 f (59-AGA GTT TGA TCM TGG CTC AG-39) and 1492 r (59- TAC GGH TAC CTT GTT ACG ACT T-39). The root length and number of lateral roots between sweet sorghum with and without Microbacterium arborescens infection showed significant differences. The length of the control roots was 9.24 cm, the treatment plants were 6.38 cm, the control plants had six treatment roots, and the treatment plants had ten lateral roots.

GC-MS Study of Bioactive Compound of Peperomia pellucida and Its Antibacterial Activity against Streptococcus mutans Kuswati, Kuswati; Rosalinda, Julitha Dwi; Waluyo, Joko
Al-Hayat: Journal of Biology and Applied Biology Vol. 8 No. 1 (2025)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v7i1.18877

Streptococcus mutans is the prior bacteria causing dental caries. Suruhan (Peperomia pellucida) may prevent dental caries due to its antibacterial bioactive content. This study aims to identify the bioactive profile of Suruhan ethanol extract and its antibacterial activity against Streptococcus mutans. Bioactive compounds were analyzed using Gas Chromatography-Mass Spectrometry (GC-MS). Antibacterial activity was tested through an inhibition test using the well-diffusion method, the Minimum Inhibitory Concentration (MIC) test, and the Minimum Bactericidal Concentration (MBC) test. The most detected compounds in Suruhan ethanol extract were n-eicosane, n-hexadecane, and glycerol. There are also antibacterial bioactives such as phenols, flavonoids, alkaloids, and terpenoids. Inhibition test of 500 mg/mL extract showed a clear zone of 8.25 mm diameter. The MIC and MBC values were 50 mg/mL and 100 mg/mL, respectively. Based on the results, Suruhan ethanol extract shows potential as an antibacterial, although its inhibitory effectiveness could be better.

[Retracted] GC-MS Study of Bioactive Compound of Peperomia pellucida and Its Antibacterial Activity against Streptococcus mutans Kuswati, Kuswati; Rosalinda, Julitha Dwi; Waluyo, Joko
Al-Hayat: Journal of Biology and Applied Biology Vol. 7 No. 1 (2024)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v7i1.18877

Streptococcus mutans is the prior bacteria causing dental caries. Suruhan (Peperomia pellucida) may prevent dental caries due to its antibacterial bioactive content. This study aims to identify the bioactive profile of Suruhan ethanol extract and its antibacterial activity against Streptococcus mutans. Bioactive compounds were analyzed using Gas Chromatography-Mass Spectrometry (GC-MS). Antibacterial activity was tested through an inhibition test using the well-diffusion method, the Minimum Inhibitory Concentration (MIC) test, and the Minimum Bactericidal Concentration (MBC) test. The most detected compounds in Suruhan ethanol extract were n-eicosane, n-hexadecane, and glycerol. There are also antibacterial bioactives such as phenols, flavonoids, alkaloids, and terpenoids. Inhibition test of 500 mg/mL extract showed a clear zone of 8.25 mm diameter. The MIC and MBC values were 50 mg/mL and 100 mg/mL, respectively. Based on the results, Suruhan ethanol extract shows potential as an antibacterial, although its inhibitory effectiveness could be better.

Molecular characterization of heavy metal tolerant bacteria from an agricultural soil Ilusanya, Olusolape Afolake Favour; Olajumoke, Oluwasegun Irouoghene; Fogbonja, Oyindolapo Christianah; Egberongbe, Haneefat Olabimpe; Oyeyipo, Funmilayo Mujidat; Yakubu, Reuben
Al-Hayat: Journal of Biology and Applied Biology Vol. 7 No. 1 (2024)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v7i1.20474

The aim of this study was to isolate and characterize heavy metal tolerant bacteria from an agricultural soil in Ago Iwoye, Ogun Stae, Nigeria. Bacteria were isolated from soil samples collected and screened for tolerance to different concentrations of lead, copper and cadmium. Heavy metal tolerant bacteria were characterized using phenotypic and molecular techniques. All the isolates were able to grow and tolerate different concentration of the heavy metals while four isolates identified as Bacillus cereus, Pseudomonas aeruginosa, Altantibacter hermannii and Enterobacter quasihormaechei exhibited high degree of tolerance to Pb, Cu and Cd with minimum inhibitory concentrations of 1500mg/l of lead,1000mg/l of Cu and 700mg/l of Cd. These results highlighted the high potential of these bacteria as bioremediation tools of heavy metal contaminated soil.Keywords: Heavy metal, tolerance, bacteria, agricultural soil

Molecular characterization of heavy metal tolerant bacteria from an agricultural soil Ilusanya, Olusolape Afolake Favour; Olajumoke, Oluwasegun Irouoghene; Fogbonja, Oyindolapo Christianah; Egberongbe, Haneefat Olabimpe; Oyeyipo, Funmilayo Mujidat; Yakubu, Reuben
Al-Hayat: Journal of Biology and Applied Biology Vol. 7 No. 1 (2024)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v7i1.20474

The aim of this study was to isolate and characterize heavy metal tolerant bacteria from an agricultural soil in Ago Iwoye, Ogun Stae, Nigeria. Bacteria were isolated from soil samples collected and screened for tolerance to different concentrations of lead, copper and cadmium. Heavy metal tolerant bacteria were characterized using phenotypic and molecular techniques. All the isolates were able to grow and tolerate different concentration of the heavy metals while four isolates identified as Bacillus cereus, Pseudomonas aeruginosa, Altantibacter hermannii and Enterobacter quasihormaechei exhibited high degree of tolerance to Pb, Cu and Cd with minimum inhibitory concentrations of 1500mg/l of lead,1000mg/l of Cu and 700mg/l of Cd. These results highlighted the high potential of these bacteria as bioremediation tools of heavy metal contaminated soil.Keywords: Heavy metal, tolerance, bacteria, agricultural soil

Optimizing Gram-Negative Bacteria Isolation from Children Diarrheal Stool Samples in Jakarta: A Comparative Analysis of Six Culture Media Abdelrazig, Omnia Amir Osman; Fadilah, Fadilah; Prasetyo, Dimas Seto; Saharman, Yulia Rosa; Hegar, Badriul
Al-Hayat: Journal of Biology and Applied Biology Vol. 7 No. 1 (2024)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v7i1.20528

The prevalence of diarrheal illnesses in Indonesia remains a significant concern among children under the age of five, evident through diagnostic findings and symptomatic manifestations, leading to increased rates of mortality and morbidity. This study aimed to identify, isolate, and culture gram-negative bacteria responsible for childhood diarrhea. Twenty stool samples were collected from children under the age of 5 with acute diarrhea in Jakarta health centres and hospitals between October 2023 to January 2024. These samples were inoculated onto selective agars to facilitate the growth of gram-negative bacteria. After a 24-hour incubation period, colonies suspected to be E. coli, K. pneumoniae, Salmonella, Shigella, or Vibrio were identified using the Vitek-2 compact system. The results revealed a notable presence of Gram-negative bacteria in all the fecal samples from diarrheal children, with Escherichia coli (E. Coli) and Klebsiella pneumoniae (Klebsiella p) as the predominant species. In conclusion, our study, utilizing various cultures and the Vitek-2 compact system, elucidates the microbial landscape driving diarrheal morbidity in Indonesian children under five, with E. Coli and klebsiella pneumoniae emerging as prevalent contributors to childhood diarrhea in this population.

Optimizing Gram-Negative Bacteria Isolation from Children Diarrheal Stool Samples in Jakarta: A Comparative Analysis of Six Culture Media Abdelrazig, Omnia Amir Osman; Fadilah, Fadilah; Prasetyo, Dimas Seto; Saharman, Yulia Rosa; Hegar, Badriul
Al-Hayat: Journal of Biology and Applied Biology Vol. 7 No. 1 (2024)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v7i1.20528

The prevalence of diarrheal illnesses in Indonesia remains a significant concern among children under the age of five, evident through diagnostic findings and symptomatic manifestations, leading to increased rates of mortality and morbidity. This study aimed to identify, isolate, and culture gram-negative bacteria responsible for childhood diarrhea. Twenty stool samples were collected from children under the age of 5 with acute diarrhea in Jakarta health centres and hospitals between October 2023 to January 2024. These samples were inoculated onto selective agars to facilitate the growth of gram-negative bacteria. After a 24-hour incubation period, colonies suspected to be E. coli, K. pneumoniae, Salmonella, Shigella, or Vibrio were identified using the Vitek-2 compact system. The results revealed a notable presence of Gram-negative bacteria in all the fecal samples from diarrheal children, with Escherichia coli (E. Coli) and Klebsiella pneumoniae (Klebsiella p) as the predominant species. In conclusion, our study, utilizing various cultures and the Vitek-2 compact system, elucidates the microbial landscape driving diarrheal morbidity in Indonesian children under five, with E. Coli and klebsiella pneumoniae emerging as prevalent contributors to childhood diarrhea in this population.

A Review of Termite for Sustainable Green Building Kusumawardhani, Dina Tiara; Almulqu, Aah Ahmad
Al-Hayat: Journal of Biology and Applied Biology Vol. 7 No. 1 (2024)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v7i1.20704

Although 3106 termite species have been documented worldwide, little information is available on those in Indonesia. In this review, the Google Scholar search platform was used to obtain information on termite species. From the 34 reviewed sources, they were identified in 4 provinces across Indonesia. Among these sites, we found 106 termite species from the islands of Sumatera, Kalimantan, Sulawesi, and Java. The most predominant species in all regions were Macrotermes sp, Microtermes sp, and Coptotermes sp.

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name
-

Contact Email
-

Phone
-

Journal Mail Official
-

Editorial Address
-

Location
Kota semarang,

Jawa tengah

INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name -

Contact Email -

Phone -

Journal Mail Official -

Editorial Address -

Location Kota semarang, Jawa tengah INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
-

Contact Email
-

Phone
-

Journal Mail Official
-

Editorial Address
-

Location
Kota semarang,

Jawa tengah

INDONESIA