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AL-HAYAT: Journal of Biology and Applied Biology
Published by Universitas Islam Negeri Walisongo Semarang
ISSN : 26543702     EISSN : 26220725     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
AL-HAYAT: Journal of Biology and Applied Biology an international journal focuses on biological science, biotechnology, bioremidiation, environmental biology, and biological education. It is intended to communicate original research and article review in current issues on the subject. The subject covers field studies and literary with various perspectives, both interdiscipline and multidiscipline, i.e. ecology, technology, education and much more. This journal is published by Fakultas Sains dan Teknologi, Universitas Islam Negeri (UIN) Walisongo Semarang Indonesia, and managed to be issued twice in every volume. We warmly welcome contributions from scholars and researchers of many disciplines.
Arjuna Subject : -
Articles 257 Documents
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Identification and Prevalence of Gastrointestinal Parasites in the Feces of Sapera Goats at Jawara Farm Arif, Hafaz; Jasmi, Riski Andrian; Rachim, Dimas Kukuh Nur
Al-Hayat: Journal of Biology and Applied Biology Vol. 8 No. 1 (2025)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v8i1.26885

Abstract

Livestock health is a crucial factor that influences the success of animal farming. One of the determinants of livestock health is the presence of parasites. This study aims to identify the types of gastrointestinal parasites and determine their prevalence in Sapera goats at Jawara Farm. The research was conducted using three examination methods: native, sedimentation, and flotation. A total of 23 goat fecal samples were examined in the laboratory. The identification results revealed five types of gastrointestinal parasites: Haemonchus contortus, Trichuris spp., Eimeria hirci, Eimeria christenseni, and Eimeria aspheronica. The parasite with the highest prevalence was Haemonchus contortus (69%), followed by Trichuris spp. (39%), Eimeria aspheronica (26%), Eimeria hirci (17%), and Eimeria christenseni (13%). The highest average Egg Per Gram (EPG) was found in Trichuris spp. (379.17), categorized as a moderate infection. Environmental factors such as temperature, humidity, and pen hygiene are strongly suspected to influence the prevalence rate and severity of parasitic infections. This study is expected to serve as a foundation for efforts in controlling and preventing parasitic infections on farms.
Aplikasi Umbi Suweg (Amorphophallus campanulatus) Sebagai Alternatif Penurun Gula Darah Pada Penderita Diabetes Mellitus Lianah, Lianah; Tyas, Dian Ayuning; Armanda, Dian Triastari; Setyawati, Siti Mukhlishoh
Al-Hayat: Journal of Biology and Applied Biology Vol. 1 No. 1 (2018)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v1i1.2666

Abstract

Penelitian ini bertujuan untuk mempelajari perbandingan kadar glukosa darah tikus putih (Rattus norvegicus L.) setelah mengonsumsi suweg mentah dan suweg rebus. Eksperimen dilakukan pada 15 ekor tikus putih sehat dengan rerata berat badan 181-183 gram. Tikus dibagi menjadi 3 kelompok perlakuan. Setiap kelompok tikus normal diadaptasikan terhadap pakan selama 3 hari dan dipuasakan selama 8-12 jam, kemudian diberi pakan sesuai dengan kelompok perlakuan masing-masing pakan suweg dengan dosis 10 mg/180 g BB suweg rebus, 10 mg/180 g BB suweg mentah, dan 10 mg/180 g BB pakan standar (kontrol).  Kadar glukosa darah diukur dengan glukometer pada menit ke 0, 60 dan 120 setelah pemberian pakan secara oral. Hasil menunjukkan bahwa perlakuan umbi suweg mentah menunjukkan aktivitas penurunan kadar gula darah lebih tinggi (3,8 mg/dl) dibandingkan umbi suweg rebus (3,6 mg/dl). Kecenderungan ini menunjukkan bahwa secara alami hormon insulin di dalam tubuh tikus sudah bekerja dalam menyeimbangkan kadar gula darah. Suweg mengandung senyawa bioaktif berupa Polisakarida Larut Air (PLA) dan serat pangan yang dapat membantu menurunkan kadar glukosa darah. Umbi suweg dapat diaplikasikan pada manusia khususnya sebagai terapi diet penurun kadar glukosa darah bagi penderita diabetes mellitus.
Isolation and Identification of Endophytic Fungi from leaves and petioles of Salam Plants (Syzygium polyanthum Wight) to Look for Antibiotic-Producing Fungi Yudiarti, Turrini; Tri Agus Sartono; Endang Widiastuti; Hanny Indrat Wahyuni; Sugiharto; Ika Agusetyaningsih
Al-Hayat: Journal of Biology and Applied Biology Vol. 8 No. 2 (2025)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

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Abstract

Synthetic antibiotics have been used to stimulate livestock growth. Because of the negative impact of using these material, it is necessary to find its substitute. One of the producer of natural antibiotics is microorganisms such as fungus. Endophytic fungi are one kinds of fungi that live in plant tissues and able to form colonies without endangering the host and can produce secondary metabolites such as antibiotic. This study is preliminary research that the ending of the purpuse is to find the endophytic fungi which producing antibiotic. This first aims of the study is to psolation and identification of the endophytic fungi found on leaves and petioles of Salam (Syzygium polyanthum Wight). The materials used are leaves and petioles of Salam which are obtained from traditional markets. The method was the isolation by direct planting used spread methods, then continue to identification. The results was the isolation of endophytic fungi from the Salam plant parts obtained as follows from leaves got five isolates and from petiole are seven isolates. Identification of all isolates found as follows from leaves are Mycelia sterilia, Acremonium sp and Fusarium sp and from petioles were Mycelia sterilia , Fusarium sp, Aspergillus sp and unidentified fungi. Keywords: endophytic fungi, antibiotics, petiols, leaves, Syzygium polyanthum
Biological Control Agent of Spodoptera frugiperda Using Bacillus thuringiensis Bacteria Indriyani, Indriyani
Al-Hayat: Journal of Biology and Applied Biology Vol. 8 No. 2 (2025)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

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Abstract

The main obstacle in the development of food crop production and horticulture is the attack of S. frugiperda larvae. Pest control using chemical insecticides has a long-term negative impact. The biological control of Spodoptera frugiperda larvae is accomplished by utilizing B. thuringiensis bacteria. This study aims to determine the best B. thuringiensis isolate for killing S. frugiperda larvae, to identify the most effective concentration of B. thuringiensis suspension, and to ascertain the mortality rate of S. frugiperda larvae. The design of this study was a randomized block design (RBD) with two factors. The first factor was the type of B. thuringiensis isolate, consisting of four isolates. Larval mortality data were analyzed using Analysis of Variance (ANOVA) and further tested with the Duncan test. The results obtained showed the highest average mortality of S. frugiperda larvae using isolates Bt3BP14 and Bt4TSR6. These two isolates had the highest average mortality on the third day. Bt3BP14 and Bt4TSR6 isolates have high potential in controlling S. frugiperda larvae, with an average mortality proportion of 86,67% and 66,67% observed over three days. The best doses of B. thuringiensis suspension for killing S. frugiperda larvae were 15 ml and 20 ml. It can be concluded that B. thuringiensis isolates can be used as natural biological control agents against S. frugiperda larvae that attack food crops and horticulture.
Plants sold in traditional marketplace in West Java highland: an ethnobotanical analysis Fitriah, Lia; Mulyanto, Dede
Al-Hayat: Journal of Biology and Applied Biology Vol. 8 No. 2 (2025)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

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Abstract

Traditional markets are crucial for sustaining plant diversity, local food systems, and cultural heritage. They also function as learning spaces for the diversity and cultural connections of food plants. This study documents the diversity and utilization of plant species traded in a traditional marketplace in West Java’s highland, Indonesia. Data were collected through stall surveys, observations, and semi-structured interviews with 38 traders. A total of 96 species from 31 families were recorded. Fabaceae and Solanaceae were the most represented families (11 species each). Vegetables were the largest category of use (47%); fruits (53%) and leaves (25%) were the most frequently used parts. Most (83%) of the plants sold were exotic, with only 21% being native species. Eight edible wild plant species, including Centella asiatica and Solanum nigrum, remain an essential part of the traditional diet. Women dominate the vegetable trade (64.51%); most vendors reported having 5–20 years of experience. These findings highlight that traditional markets play a crucial role in preserving and serving as learning spaces for plant diversity, local food systems, and cultural heritage.
Isolation and Molecular Identification of Cadmium-Resistant Bacteria from Paddy Soil in the Tallo Watershed, Makassar Nurhikmayani, Risky; Dewi, Aghis Sukma
Al-Hayat: Journal of Biology and Applied Biology Vol. 8 No. 2 (2025)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

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Abstract

Cadmium contamination of paddy soils is a global concern because Cd can accumulate in rice through nutrient absorption, posing a food safety risk. More than a quarter of the Tallo watershed area, Makassar is a rice field that is vulnerable to pollution due to industrial activities and water runoff from urban areas. This study aims to isolate and identify Cd-resistant bacteria from paddy field soils in the Tallo watershed to find potential isolates that have the potential for bioremediation. The soil samples collected were from the rhizosphere of rice fields in the Tallo watershed, which were varying distances from the Tallo Main River. Bacterial isolation used nutrient agar media, followed by Cd resistance testing using Nutrient Agar-CdCl₂ media with increasing concentrations from 10 mg L-1 to 110 mg L-1. Molecular identification was carried out on the most Cd-tolerant isolates using the 16S rRNA marker gene with primers 27F and 1492R. From the isolation, 22 isolates were obtained, with 19 isolates resistant to CdCl2 10 mg L-1; Only five isolates could continue growing at a concentration of 100 mg L-1, and only one isolate could grow up to a concentration of 110 mg L-1; the isolate was L1(4). The isolate L1(4) exhibited the best growth and was identified as Aeromonas veronii through 16S rRNA gene sequencing. These findings highlight Aeromonas veronii as a promising candidate for the bioremediation of Cd-contaminated soils in the Tallo watershed
Developmental Anatomy of Anther in the Red Dragon Fruit (Hylocereus polyrhizus Britton & Rose) Utaminingsih, Utaminingsih
Al-Hayat: Journal of Biology and Applied Biology Vol. 8 No. 2 (2025)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

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Abstract

The developmental anatomy of the anther in Hylocereus polyrhizus (red dragon fruit) was examined to elucidate the sequential processes of microsporogenesis and microgametogenesis that underlie pollen formation. Flower buds were sampled at five developmental stages (1-5) and analyzed histologically using paraffin embedding and safranin staining. Anther length increased progressively by 96% from stages 1 to 4 and plateaued before anthesis, indicating growth cessation before pollen maturation. The anther wall followed a dicotyledonous pattern, comprising the epidermis, endothecium, middle layer, and tapetum. Early stages featured archesporial differentiation into primary parietal and sporogenous cells, followed by the formation of secondary parietal and sporogenous layers. The endothecium developed fibrous thickenings, while the binucleate, secretory tapetum provided nutrients for developing microspores and degenerated at maturity. Microsporogenesis proceeded from microspore mother cells to tetrads and solitary microspores, culminating in tricellular pollen grains at anthesis. These findings demonstrate that anther development in H. polyrhizus conforms to the typical dicotyledonous type, with characteristic features such as a secretory tapetum and fibrous endothecium. The results provide essential anatomical insight to support breeding programs, artificial pollination strategies, and reproductive biology studies of this economically valuable cactus species.

Page 26 of 26 | Total Record : 257

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