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Antioxidant Activity Of The Peel Citrus sinensis. L On The Histological Features Of Second Degree Burned Mus musculus Samsudin, Rinza Rahmawati; Arimurti, Anindita Riesti Retno; Puspitasari, Renny Novi
Al-Hayat: Journal of Biology and Applied Biology Vol. 7 No. 2 (2024)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v7i2.17580

Burns are damage to the tissue that does not only occur on the surface of the skin, but can occur under the skin. Globally, burns are the fourth most common type of injury, after traffic accidents, falls and physical abuse. The cost of treating burns is relatively expensive according to the area of the burn, the larger the area of the burn, the higher the cost of treatment. Research for the treatment of burns using herbal ingredients has begun to be carried out by many researchers. One of the typical Indonesian herbal plant ingredients is the Pacitan orange (Citrus sinensis) L Osbeck. This type of research is an experimental research with the aim of looking at the formation of epithelial cells, fibroblasts, and collagen which are formed after being given Pacitan orange peel extract (Citrus sinensis) treatment. The sample of this study used 20 white rats which were divided into 4 groups, namely Group 1 (K1) the burn group without treatment, Group 2 (K2) the burn rats with bioplacenton treatment, Group 3 (K3) the burn rats with NaCl treatment 0.9%, Group 4 (K4) burnt rats treated with extra 100% Pacitan orange peel. From the research results obtained, it can be concluded that the administration of Pacitan Orange Peel extract is proven to accelerate the healing process of second degree burns on the skin of white rats viewed microscopically, namely from increased collagen production, epithelial thickness, and fibroblasts.Keywords: Pacitan orange peel extract, Burns, Mus musculus, histopathology

Community Structure and Macroalgae Diversity in Peh Pulo Beach, Blitar Regency Sofiyana, Marinda Sari; Sholihah, Mar’atus; Setyaningsih, Dewi
Al-Hayat: Journal of Biology and Applied Biology Vol. 6 No. 2 (2023)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v6i2.17767

The coastal area of Peh Pulo Beach, located in the Blitar Regency, has a high potential for biodiversity, including macroalgae. This research aims to understand the community structure and diversity of macroalgae at Peh Pulo Beach. The sampling was conducted on August 2023 using the 1 x 1 m quadrant transect method. Macroalgae were collected at three observation stations, with three plots observed at each station. Abiotic environmental parameters measured included temperature, salinity, pH, light intensity, and substrate. The results of macroalgae identification at Peh Pulo Beach reveal 21 species belonging to 16 genera and 14 families. These species were classified into Chlorophyta (9 species), Phaeophyta (4 species), and Rhodophyta (8 species). The Shannon Diversity Index value obtained is 2.84, indicating that the macroalgae diversity along the coast of Peh Pulo falls under the moderate category.

Community Structure and Macroalgae Diversity in Peh Pulo Beach, Blitar Regency Sofiyana, Marinda Sari; Sholihah, Mar’atus; Setyaningsih, Dewi
Al-Hayat: Journal of Biology and Applied Biology Vol. 6 No. 2 (2023)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v6i2.17767

The coastal area of Peh Pulo Beach, located in the Blitar Regency, has a high potential for biodiversity, including macroalgae. This research aims to understand the community structure and diversity of macroalgae at Peh Pulo Beach. The sampling was conducted on August 2023 using the 1 x 1 m quadrant transect method. Macroalgae were collected at three observation stations, with three plots observed at each station. Abiotic environmental parameters measured included temperature, salinity, pH, light intensity, and substrate. The results of macroalgae identification at Peh Pulo Beach reveal 21 species belonging to 16 genera and 14 families. These species were classified into Chlorophyta (9 species), Phaeophyta (4 species), and Rhodophyta (8 species). The Shannon Diversity Index value obtained is 2.84, indicating that the macroalgae diversity along the coast of Peh Pulo falls under the moderate category.

Callus Induction from Stem Explants of Binahong (Anredera cordifolia (Ten.) Steenis) with the Addition of Picloram and BAP Aulia, Siska Na'ilil; Habibah, Noor Aini
Al-Hayat: Journal of Biology and Applied Biology Vol. 7 No. 1 (2024)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v7i1.17799

The many benefits of medicinal plants require development techniques that aim to produce high and diverse secondary metabolite contents. The purpose of this study was to analyze the effect of the concentration of picloram and BAP and determine the optimal concentration in inducing binahong callus. The research design used was a completely randomized design with two factors, namely the combination of picloram and BAP (0, 1, 2, and 3 ppm). The incubation process of binahong callus was carried out for 40 days after planting (DAP). The observed callus growth indicators consisted of days to callus formation (days), percentage of callus explants (%), callus morphology (texture and color), and callus diameter. Data on days to callus formation and frequency of callus explants were analyzed using the Kruskal-Wallis test and further tested by Dunn if there was a significant effect. The results showed that picloram significantly affected the parameters of days to callus formation and the percentage of callus explants, while BAP significantly affected the parameters of days to callus formation and did not significantly affect the percentage of callus explants parameter. At concentrations of 1 ppm, 2 ppm, and 3 ppm, picloram had a significant effect on both the days to callus formation and the percentage of callus explants parameters. BAP at concentrations of 1 ppm, 2 ppm, and 3 ppm only had a significant effect on the parameter of days to callus formation appearance and was not significant in the percentage of callus explants parameter. The interaction between BAP and picloram had a significant effect on the parameters of days to callus formation and the percentage of callus explants. The resulting callus has a compact texture with callus colors ranging from white, and green, to brown. The most optimal concentration in all indicators is 0 ppm BAP + 1 ppm picloram with days to callus formation of 6 DAP, 100% explants form callus, overall callus is white with compact texture, and has a very high callus growth.

Callus Induction from Stem Explants of Binahong (Anredera cordifolia (Ten.) Steenis) with the Addition of Picloram and BAP Aulia, Siska Na'ilil; Habibah, Noor Aini
Al-Hayat: Journal of Biology and Applied Biology Vol. 7 No. 1 (2024)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v7i1.17799

The many benefits of medicinal plants require development techniques that aim to produce high and diverse secondary metabolite contents. The purpose of this study was to analyze the effect of the concentration of picloram and BAP and determine the optimal concentration in inducing binahong callus. The research design used was a completely randomized design with two factors, namely the combination of picloram and BAP (0, 1, 2, and 3 ppm). The incubation process of binahong callus was carried out for 40 days after planting (DAP). The observed callus growth indicators consisted of days to callus formation (days), percentage of callus explants (%), callus morphology (texture and color), and callus diameter. Data on days to callus formation and frequency of callus explants were analyzed using the Kruskal-Wallis test and further tested by Dunn if there was a significant effect. The results showed that picloram significantly affected the parameters of days to callus formation and the percentage of callus explants, while BAP significantly affected the parameters of days to callus formation and did not significantly affect the percentage of callus explants parameter. At concentrations of 1 ppm, 2 ppm, and 3 ppm, picloram had a significant effect on both the days to callus formation and the percentage of callus explants parameters. BAP at concentrations of 1 ppm, 2 ppm, and 3 ppm only had a significant effect on the parameter of days to callus formation appearance and was not significant in the percentage of callus explants parameter. The interaction between BAP and picloram had a significant effect on the parameters of days to callus formation and the percentage of callus explants. The resulting callus has a compact texture with callus colors ranging from white, and green, to brown. The most optimal concentration in all indicators is 0 ppm BAP + 1 ppm picloram with days to callus formation of 6 DAP, 100% explants form callus, overall callus is white with compact texture, and has a very high callus growth.

Arthropod diversity in three different habitats around Sumbawa University Of Technology, Sumbawa, NTB Fikri, Galih El; Arrufitasari, Putri Nur
Al-Hayat: Journal of Biology and Applied Biology Vol. 7 No. 1 (2024)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v7i1.18182

Arthropods have an important role in a habitat and food chain cycle. Identification of arthropod species abundance and diversity is done as a biological indicator of the environment. The aim of the research is determine the diversity and community structure of soil arthropods in various habitats and provide information on environmental quality caused by human activities around the campus of Sumbawa University of Technology. This research was conducted in three locations in the area around the Sumbawa University of Technology, Batu alang village, Moyo Hulu sub-district, Sumbawa district. The three locations include hillsides, campus areas and corn fields. Sampling of soil arthropods using the pitfall trap technique. Data on abundance, species richness, diversity, Calculation of the Index of Importance (INP) and diversity value (H') was analized by Excel. The results of Arthropod identification found a total of 2083 individuals consisting of 20 families and 8 Arthropod orders. The three sites showed that the abundance of arthropods in campus area was the highest at (212.75 ± 9.54) Arthropod species richness in corn field was the highest at 2.67 ± 0.4 Arthropod diversity (H’) in hilside was the highest at (3.23 ± 0.16). Campus area and corn field which have higher similarity.

Arthropod diversity in three different habitats around Sumbawa University Of Technology, Sumbawa, NTB Fikri, Galih El; Arrufitasari, Putri Nur
Al-Hayat: Journal of Biology and Applied Biology Vol. 7 No. 1 (2024)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v7i1.18182

Arthropods have an important role in a habitat and food chain cycle. Identification of arthropod species abundance and diversity is done as a biological indicator of the environment. The aim of the research is determine the diversity and community structure of soil arthropods in various habitats and provide information on environmental quality caused by human activities around the campus of Sumbawa University of Technology. This research was conducted in three locations in the area around the Sumbawa University of Technology, Batu alang village, Moyo Hulu sub-district, Sumbawa district. The three locations include hillsides, campus areas and corn fields. Sampling of soil arthropods using the pitfall trap technique. Data on abundance, species richness, diversity, Calculation of the Index of Importance (INP) and diversity value (H') was analized by Excel. The results of Arthropod identification found a total of 2083 individuals consisting of 20 families and 8 Arthropod orders. The three sites showed that the abundance of arthropods in campus area was the highest at (212.75 ± 9.54) Arthropod species richness in corn field was the highest at 2.67 ± 0.4 Arthropod diversity (H’) in hilside was the highest at (3.23 ± 0.16). Campus area and corn field which have higher similarity.

Ethnobotani of Suweg (Amorphophallus Paeoniifolius (Dennst.) Nicolson) in Darupono Village, Kendal District Dinalhaq, Putri Cahya; Wahidah, Baiq Farhatul; Miswari, Miswari
Al-Hayat: Journal of Biology and Applied Biology Vol. 6 No. 2 (2023)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v6i2.18573

Suweg (Amorphophallus paeoniifolius (Dennst.) Nicolson) merupakan tumbuhan liar yang tumbuh dan dimanfaatkan oleh masyarakat desa Darupono. Penelitian ini bertujuan untuk mengetahui pemanfaatan tanaman suweg di Desa Darupono, organ tanaman suweg apa saja yang dimanfaatkan, dan potensi tanaman suweg, serta mengetahui cara budidaya tanaman suweg di Desa Darupono. Penelitian ini tergolong penelitian deskriptif eksploratif dengan menggunakan metode survei, wawancara semi terstruktur, dan menggunakan data pendukung yaitu kuesioner. Pengumpulan data dilakukan dengan teknik acak sederhana dan snowball sampling. Penelitian yang dilakukan di Desa Darupono menunjukkan bahwa tanaman suweg memiliki beberapa potensi antara lain mencegah diabetes, antibakteri, dan antikanker, serta dapat diolah menjadi tepung suweg dan bahan dasar pembuatan mie. Tanaman suweg dimanfaatkan oleh masyarakat Desa Darupono sebagai bahan pangan yaitu dengan cara dikukus, direbus, digoreng, dan diolah menjadi emping, com, pote dan sayur daun oblok-oblok suweg . Selain itu, suweg juga digunakan untuk upacara adat tujuh bulanan atau dalam bahasa Jawa disebut mitoni. Masyarakat Desa Darupono membudidayakan suweg dengan menanam umbi-umbian. Tahapan budidaya suweg adalah penyiapan lahan, penyiapan benih, penanaman, pemeliharaan dan pemanenan.

Ethnobotani of Suweg (Amorphophallus Paeoniifolius (Dennst.) Nicolson) in Darupono Village, Kendal District Dinalhaq, Putri Cahya; Wahidah, Baiq Farhatul; Miswari, Miswari
Al-Hayat: Journal of Biology and Applied Biology Vol. 6 No. 2 (2023)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v6i2.18573

Suweg (Amorphophallus paeoniifolius (Dennst.) Nicolson) merupakan tumbuhan liar yang tumbuh dan dimanfaatkan oleh masyarakat desa Darupono. Penelitian ini bertujuan untuk mengetahui pemanfaatan tanaman suweg di Desa Darupono, organ tanaman suweg apa saja yang dimanfaatkan, dan potensi tanaman suweg, serta mengetahui cara budidaya tanaman suweg di Desa Darupono. Penelitian ini tergolong penelitian deskriptif eksploratif dengan menggunakan metode survei, wawancara semi terstruktur, dan menggunakan data pendukung yaitu kuesioner. Pengumpulan data dilakukan dengan teknik acak sederhana dan snowball sampling. Penelitian yang dilakukan di Desa Darupono menunjukkan bahwa tanaman suweg memiliki beberapa potensi antara lain mencegah diabetes, antibakteri, dan antikanker, serta dapat diolah menjadi tepung suweg dan bahan dasar pembuatan mie. Tanaman suweg dimanfaatkan oleh masyarakat Desa Darupono sebagai bahan pangan yaitu dengan cara dikukus, direbus, digoreng, dan diolah menjadi emping, com, pote dan sayur daun oblok-oblok suweg . Selain itu, suweg juga digunakan untuk upacara adat tujuh bulanan atau dalam bahasa Jawa disebut mitoni. Masyarakat Desa Darupono membudidayakan suweg dengan menanam umbi-umbian. Tahapan budidaya suweg adalah penyiapan lahan, penyiapan benih, penanaman, pemeliharaan dan pemanenan.

Authentication of Wax Apple (Syzygium samarangense (Blume) Merr. & L.M Perry) Delima and Citra Cultivars by Morphological and Molecular Approach Mukaromah, Arnia Sari; Ulfah, Malia; Rachmah, Annisa Nur; Arfan, Muhammad Ramdhani; Kusumarini, Niken; Febriana, Asri
Al-Hayat: Journal of Biology and Applied Biology Vol. 6 No. 1 (2023)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v6i1.18713

Wax apple Delima and Citra Cultivars are two superior non-climacteric tropical fruit commodities from Demak Regency which have similar morphological characters but have different fruit characteristics. Identification wax apple cultivars from Demak regency using DNA barcoding approach has not been researched yet. The aims of this research are to analyze the morphological similarities of wax apple between Delima and Citra cultivars and to identify genetic variations of wax apple Delima and Citra using trnL-trnF intergenic spacer for molecularly authentication. The results shown that there were no differences in the environmental parameters of the wax apple two cultivated area in Demak Regency. According to the morphological approach, the Delima and Citra cultivars in Demak Regency were grouped separately into Delima and Citra clusters with a similarity index of 61.5% (Citra cultivars) and 60.5% (Delima cultivars) and separated based on the origin of their cultivation area. Genetic variations between wax apple Delima and Citra in Demak Regency consisted of deletions (9delA & 17delA) and conserved P6 loop in all compared cultivars. The possibility of heteroplasmy R (A,G) found at 73 nucleotide number in Delima Betokan cultivar and Citra cultivars (Betokan and Jungpasir). Meanwhile, Delima Jungpasir cultivar is K (G,T). There were P8 stem-loops with different lengths between Delima cultivars (Betokan and Jungpasir) and the same length in Citra cultivars (Betokan and Jungpasir). Therefore, the trnL-trnF intergenic spacer has not been thoroughly used in wax apple Delima and Citra authentication due to the presence of a secondary structure which causes the loss of the electropherogram signal so that the nucleotide sequence cannot be read.

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Kota semarang,

Jawa tengah

INDONESIA