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INDONESIA
Indonesian Journal of Natural Pigments
Published by Ma Chung Research Center for Photosynthetic Pigments Publishing
ISSN : 23389281     EISSN : 26214245     DOI : -
Core Subject : Science, Education,
Chemistry Other
Indonesian Journal of Natural Pigments (Indones. J. Nat. Pigm.) is a double-blind international peer-review and open access journal (p-ISSN: 2338-9281, e-ISSN: 2621-4245) covering all aspects of natural pigments including biodiversity of natural pigment resources (isolation and analysis of photosynthetic pigments), pigments for food and healthcare, pigments for energy and pigments for sensors.
Arjuna Subject : -
Articles 6 Documents
 1 
Search results for , issue "Vol 2 No 2 (2020): Agustus 2020" : 6 Documents clear
Color Characteristics and Stability of Anthocyanin in Fresh Thunbergia erecta Flower Extract Marpaung, Abdullah Muzi; Ramdhani, Rizal Pauzan
Indonesian Journal of Natural Pigments Vol 2 No 2 (2020): Agustus 2020
Publisher : Ma Chung Research Center for Photosynthetic Pigments

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33479/ijnp.2020.02.02.31

Abstract

The main purpose of the present research was to evaluate the color characteristics of anthocyanin in Thunbergia erecta flower extract at various pH to know its potentiality as a food colorant. The fresh petal was macerated in 0.1 N HCl-Ethanol 96% (1:9) for an hour, filtrated and diluted in a buffer solution pH 1 to 12, then scanned by a spectrophotometer at 400 – 700 nm. The extract was almost colorless at pH 4 to 6 and show extremely low color stability at pH 7 and 8 (the half-life was 6.69 and 16.66 minutes, respectively). In contrast, the extract was very stable at pH 3. There was no significant color decrease after the extract being stored for 14 days at pH 3. An important spectral characteristic of Thunbergia erecta flower extract appeared at pH 7 to 10 by showing all the three colored species of anthocyanin. At pH 8, the red flavylium cation detected as λshoulder at around 537 nm, the purple quinonoidal base as the λpeak at 577 nm and blue anionic quinonoidal base as the λpeak at 614 nm. The unique spectral characteristic promoted the Thunbergia erecta flower as a potential anthocyanin-source to be used in the study of anthocyanin transformation in an aqueous system.
Activity Antioxidant on Pigments of Bacterial Symbionts of Soft Coral From Jepara Sea Masduqi, Ahmad Fuad; Franyoto, Yuvianto Dwi; Kusmita, Lia; Muchlisin, Sakti; Widyananto, Prasetyo Abi; Sulistyani, Sulistyani; Wijayanti, Diah Permata
Indonesian Journal of Natural Pigments Vol 2 No 2 (2020): Agustus 2020
Publisher : Ma Chung Research Center for Photosynthetic Pigments

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33479/ijnp.2020.02.02.43

Abstract

Soft corals have been known to produce secondary metabolites, some of which may have anticancer, antifouling, antibacterial and antioxidants activity. It has been suggested that natural products from marine invertebrates have striking similarities to metabolites of their association microorganisms. Symbiont bacteria on soft coral can produce bioactive compounds that play an important role in chemical ecology and as a marine natural product. Marine bacteria associated with soft coral collected from Jepara were successfully isolated on medium ZoBell 2216E and screened to synthesize the pigment. This approach has allowed the use of this organism as an environmentally friendly alternative source of new natural pigment. This study found 25 bacterial isolates from 6 types of soft coral. Out of 25 bacterial isolates, only 3 bacterium, positively contains pigments. Four isolates, PCl 1, PS2 1, and PSa 2. Pigments analysis with UV spectrophotometric method showed the wavelength of pigments were in the range 300-600 nm. Genomic DNA was isolated from these colonies and nested PCR of the DNA was performed to amplify the 16S rDNA. Antioxidant activity was tested with the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. From the results of molecular identification by 16S rDNA method, it was shown that bacterium PCl 1, PS2 1, and PSa 2 was closely related to Pseudomonas stutzeri, Ponticoccus gilvus, Bacillus marisflavi with 99%, 99and 98% homology value. Antioxidant activity is as follows: PCl 1>PS2 1>PSA 2.
Carotenoid Production by Rhodosporidium paludigenum Using Cassava Starch Hydrolyzed by Bacillus subtilis as Substrate Warjoto, Renna Eliana; Felianti, Felianti; Lay, Bibiana Widiyati
Indonesian Journal of Natural Pigments Vol 2 No 2 (2020): Agustus 2020
Publisher : Ma Chung Research Center for Photosynthetic Pigments

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33479/ijnp.2020.02.02.36

Abstract

Carotenoids are natural pigments with colors ranging from yellow to red that are beneficial for food, cosmetics, and animal feed industries. These pigments can be found in fruits, vegetables, algae, and microorganisms. Among all microorganisms that have been known to produce carotenoids, Rhodosporidium paludigenum is still poorly investigated. Therefore, this study aimed to determine the potential of carotenoid production by R. paludigenum using cassava starch hydrolyzed by Bacillus subtilis as a substrate. The cassava starch for hydrolysis was divided into four concentrations, i.e., 2%, 4%, 6%, and 8% w/v. During the hydrolysis period, the amylase enzyme activity produced by B. subtilis was evaluated. The reducing sugar concentration was then examined to determine the optimum medium for carotenoid production. The highest amylase enzyme activity was produced on the second day in all cassava starch concentrations. However, the highest reducing sugar concentration was discovered in the 6% w/v cassava starch concentration. Thus, a batch submerged fermentation for carotenoid production by R. paludigenum was performed using the hydrolysate as the sole substrate. At the end of the fermentation, the total carotenoid was extracted, and the concentration was determined using spectrophotometry. The total yield of xanthophyll over biomass was higher than that of β-carotene. These findings elucidated the potency of cassava starch hydrolysate obtained from the starch hydrolyzed by B. subtilis, for carotenoid production by the red yeast R. paludigenum.
Humans’ Body Immunity and Natural Pigments Indrawati, Renny; Limantara, Leenawaty; Brotosudarmo, Tatas H.P.
Indonesian Journal of Natural Pigments Vol 2 No 2 (2020): Agustus 2020
Publisher : Ma Chung Research Center for Photosynthetic Pigments

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33479/ijnp.2020.02.02.26

Abstract

The emerging pandemic of Corona Virus Disease (COVID-19) has attracted special concerns regarding the case of very contagious viral infection. Besides the profound waiting toward vaccine development, public awareness was also addressed into the effort of increasing humans’ body immunity. Nowadays, the people are encouraged to consume adequate amount of vitamin C and increase the consumption of fruits and vegetables. In addition, various kinds of food supplements are offered and claimed to give us protection and prevention against viral infection by boosting our immune system. However, the communities are actually lack of proper information regarding the true mechanism of immune system. How is the infection developed in our body? How does the immune system work? Could it be modulated by consuming the bioactive compounds? Here, the stages of infection, the immune system, as well as the role of natural pigments to support the humans’ body immunity will be briefly discussed.
Color Alteration of Encapsulated Beetroot (Beta vulgaris L.) Extract Upon Dissolving in Various pH Treatment Lukitasari, Diah Mustika; Indrawati, Renny; Chandra, Rosita Dwi; Heriyanto, Heriyanto; Limantara, Leenawaty
Indonesian Journal of Natural Pigments Vol 2 No 2 (2020): Agustus 2020
Publisher : Ma Chung Research Center for Photosynthetic Pigments

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33479/ijnp.2020.02.02.48

Abstract

It has been known that most natural pigments are unstable upon exposure against several environmental factors, such as thermal treatment, light, oxidizing or reducing agents, as well as acid or alkaline compounds. Encapsulation procedures is often adopted to adjust the solubility and provide protection to the natural pigments. Here, we prepared an encapsulated beetroot extract as the candidate of red bio-colorant. The primary aim of the present study is to investigate color alteration of encapsulated beetroot (Beta vulgaris L.) extract upon dissolving in various pH adjustment and prolonged storage. The McIllvainne buffer was prepared in various pH range, i.e. from 2 to 11. The encapsulated extract (0.1% w/v) was dissolved and the color of the solutions were measured regularly until 3 days storage under darkness at 200C. Any degradation or structural changes will cause color alteration, which were monitored through L*, a*, b* values, the hue angle (H°), chroma values (C), as well as color difference (DE). The results showed that pH 4 was the most favorable condition that brings least impact to the color alteration, even when the colored solution was kept in prolonged storage until 9 days.
High Antioxidant Activity of Pucuk Merah (Syzygium oleina) Leaf and Zinnia (Zinnia elegans) Flower Extracts Purnomo, Tantyo Ardy Bintoro; Yuliati, Leny
Indonesian Journal of Natural Pigments Vol 2 No 2 (2020): Agustus 2020
Publisher : Ma Chung Research Center for Photosynthetic Pigments

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33479/ijnp.2020.02.02.54

Abstract

A study on the antioxidant activity of natural sources is very crucial to explore their benefits, especially for food, medicine, as well as cosmetic applications. In this work, the antioxidant activities of pucuk merah (Syzygium oleina) leaves and zinnia (Zinnia elegans) flower were examined. Pucuk merah leaves and zinnia flowers were extracted using an acidic ethanol solvent by a simple maceration method for 24 hours. The color of the obtained pucuk merah leaf extract was red, while the zinnia flower extract was yellowish-brown. UV-visible spectrophotometer showed that the pucuk merah leaf extract has anthocyanin as the main part and chlorophyll as the minor part. On the other hand, zinnia flower extract contained carotenoid as the main part, as well as small parts of anthocyanin and chlorophyll. The antioxidant activity test was carried out by using 2-2-diphenyl-1-picrylhydrazyl (DPPH) as the antioxidant assay. It was revealed that the inhibitory concentration at 50% (IC50) values of the pucuk merah leaf and zinnia flower extracts were 25.83 and 20.16 mg/L, respectively, while the antioxidant activity index (AAI) of the pucuk merah leaf and zinnia flower extracts were 1.55 and 1.98, respectively. These results showed that both pucuk merah leaf and zinnia flower extracts have strong antioxidant activity, and thus very potential to be used as great antioxidants.

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