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INDONESIA
Jurnal Entomologi Indonesia
Published by Perhimpunan Entomologi Indonesia
ISSN : 18297722     EISSN : 20890257     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Jurnal Entomologi Indonesia (JEI) publishes original research papers and short communications that covers the basic and applied aspect of insects and mites or other arthropods in agriculture, forestry, human and animal health, and natural resources and environmental management. The journal is published by Perhimpunan Entomologi Indonesia (Entomological Society of Indonesia). JEI was first published at the beginning of 2004 with twice per year in frequency on March and September. Since 2015, Jurnal Entomologi Indonesia has been published three times per year on March, July, and November.
Arjuna Subject : -
Articles 6 Documents
 1 
Search results for , issue "Vol 22 No 1 (2025): March" : 6 Documents clear
Butterfly diversity in natural and modified habitat at Bahorok District, Langkat Regency, North Sumatra: Keanekaragaman kupu-kupu di habitat alami dan habitat buatan di Kecamatan Bahorok, Kabupaten Langkat, Sumatera Utara Nurhayati; Syarifuddin; Ritonga, Yusran Efendi; Pradwinata, Ricky; Pendong, Lexi Majesty
Jurnal Entomologi Indonesia Vol 22 No 1 (2025): March
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.22.1.17

Abstract

Changes in the function of natural areas, rice fields, and plantations can reduce the number and diversity of butterflies, with environmental factors playing a significant role in shaping butterfly richness and diversity. This study aimed to identify differences in butterfly diversity and composition between natural and modified habitats and to examine the relationship between environmental factors and butterfly richness and diversity. This research was conducted from March to April 2021 using a modified exploration method (pollard walk) through direct observations of butterflies. Observations were conducted in two sessions from 08:00–12:00 and 13:00–17:00. The results of this study successfully found five butterfly families with 995 individuals in natural habitats (79 species), whereas, in modified habitats, as many as 627 individuals (29 species) were documented. Based on the Shannon Winner index (H’) and Margelaf richness index (R), the natural habitat was classified as high (H’ = 3.84, R = 11.2). Simpson’s index and evenness values of the two habitats were too different and classified as high. There were significant differences in butterfly abundance between the natural and modified habitats (t (164) = 2.441, p = 0.016). Among the biotic factors examined, only wind speed significantly affected butterfly abundance.
Aktivitas insektisida ekstrak Piper aduncum dan Aglaia odorata terhadap Plutella xylostella (Linnaeus) (Lepidoptera: Plutellidae): Insecticidal activity of Piper aduncum and Aglaia odorata extracts on Plutella xylostella (Linnaeus) (Lepidoptera: Plutellidae) heviyanti, Maria; Dadang; Sartiami, Dewi; Kusumah, Yayi Munara; Purwantiningsih
Jurnal Entomologi Indonesia Vol 22 No 1 (2025): March
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.22.1.52

Abstract

One of the main problems in the cultivation of Brassicaceae vegetable plants is the attack of diamondback moth Plutella xylostella (Linnaeus). Extracts of Piper aduncum and Aglaia odorata have the potential to be used as botanical insecticides because they contain compounds that are toxic to insects. This study aims to evaluate the toxicity and feeding inhibition activity of hexane-extract of P. aduncum fruits and methanol-extract of A. odorata twigs against second instar larvae of diamondback moth. Extraction was carried out using the maceration method with a powder-to-solvent ratio of 1:10 (w/v). The diamondback moth insects used were from colonies reared in the laboratory. The lethal effect test was conducted using the leaf-dip method with test concentrations equivalent to LC15, LC35, LC55, LC75, LC95, and a control. The feeding inhibition test was conducted using the choice-test method with test concentrations equivalent to LC15, LC35, LC55, and a control. The results show that the hexane-extract of P. aduncum fruit is more toxic to second instar P. xylostella larvae than the methanol-extract of A. odorata twigs based on the probit analysis value of the relationship between concentration and mortality. The LC50 and LC95 values of P. aduncum extract were 0.07% and 0.14%, respectively, while the LC50 and LC95 values of A. odorata extract were 1.63% and 4.72%. Additionally, P. aduncum and A. odorata extracts also exhibited feeding against diamondback moth at concentrations of 0.07% and 1.77%, respectively. The combined effects of direct toxicity and feeding inhibition contributed to the mortality of the test insects. Thus, these two extracts, escpecially P. aduncum extract wich demonstrated higher effectiveness, have a great potential as botanical insecticides for controlling diamondback moth in Indonesia.
Waktu kritis periode lunar dalam menggambarkan status reproduksi ngengat Scirpophaga incertulas (Walker) pada berbagai fase pertumbuhan padi: Critical timing of the lunar period in describing the reproductive status of the Scirpophaga incertulas moth (Walker) on several growth stage of rice Anggraeni, Flavia Devi; Anwar, Ruly; Ratna, Endang Sri
Jurnal Entomologi Indonesia Vol 22 No 1 (2025): March
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.22.1.29

Abstract

The yellow stem borer, Scirpophaga incertulas (Walker) is a major nocturnal pest of rice. Presence of S. incertulas moth had a flight peak during the lunar period and affects its reproductive status. The purpose of this study was to analyze the critical time in the lunar period related to the highest reproductive status of S. incertulas in the field. Monitoring of moth populations were carried out by setting up two traps equipped with 60-watt LED lights (800 lm) on farmers’s fields in Rawamerta District, Karawang Regency, West Java. Moth trapping was carried out once an hour, started 6–10 PM, for three days on lunar period. The observation were conducted during the nursery, maximum tillering, and grain filling phases. upen capture, females were transferred to rearing cages until they laid all their eggs. Moth reproductive status, fecundity, and fertility were counted and analyzed. Total of captured female S. incertulas moths in the lunar period from three phases of rice was 216 individuals. In the nursery phase, the highest population level was obtained at 8 PM at 27.8%, While the maximum tillering and grain filling phase were 39.3 and 29.1% at 7 PM. The highest population level of virgin, mating, and post-oviposition moths was obtained at 7 PM, at 10,94, 12,52, and 9,64%. The highest average fecundity occurred at 6 PM with 42 eggs/group and the highest fertility was 45,8% at 8 PM. The highest critical reproductive time of female S. incertulas moths occured between 6–8 PM.
Keanekaragaman strain ulat grayak jagung Spodoptera frugiperda (Smith) berdasarkan marker COI dan Tpi dengan kajian kepadatan populasi di Bali: Genetic diversity of fall armyworm Spodoptera frugiperda (Smith) strains based on COI and Tpi markers with population density analysis in Bali Monica, Dheya Cintya; Kusumah, Yayi Munara; Winasa, I Wayan
Jurnal Entomologi Indonesia Vol 22 No 1 (2025): March
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.22.1.41

Abstract

Spodoptera frugiperda (Smith), known as fall armyworm (FAW), originating from America that devastates corn crop, causing up to 50% damage to corn crops in Bali. Molecular identification of strain diversity using mitochondrial cytochrome oxidase subunit I gene (mtCOI) and triosephosphate isomerase gene (Tpi) markers also population density monitoring are a fundamental method for monitoring, detecting and controlling these pests. This research aims to determine FAW strain based on COI and Tpi markers and their population density analysis at representative sites in Bali. This study involved several stages: samples collection; DNA extraction; amplification, visualization; Population density observation and sequencing; and data analysis. Data were analyzed using GeneStudio and BioEdit for editing and alignment, and MEGA 11 for constructing phylogenetic tree. Sequence analysis based on COIB indicated that samples from Bali comprised of 41.67% rice strains and 58.33% corn strains, with 100% of corn strain  haplotypes being h4 FAW [FL] from Florida. Based on Tpi marker, 100% of the samples were identified as corn strains with haplotypes Ca1 and Ca2. This study found that the FAW diversity in Bali consists of two strains and one haplotype based on COI (COI-R and COI-Ch4), while based on Tpi, there is only one strain  with two haplotypes (Tpi-Ca1 and Tpi-Ca2). The highest population density of FAW was observed in the Tabanan plot, with 6,8 larvae/sample unit. Early instar larvae (2–3) were predominant in 3 WAP whereas late instar larvae (4–6) were predominant in 5 WAP.
Eksplorasi potensi budi daya lebah madu Apis cerana Fabricius di Taman Kehati Kiarapayung, Jawa Barat: Exploration of the potential of Apis cerana Fabricius beekeeping in the Kiarapayung Biodiversity Park, Jawa Barat Asfiya, Wara; Winara, Aji; Prativi, Mahensa Billqys Nurhayati; Ruswandi, Agus; Narakusumo, Raden Pramesa; Encilia; Sumadijaya, Alex; Kahono, Sih; Darmawan; Sarino; Budiasih, Yulie; Hapsari, Laras Ay
Jurnal Entomologi Indonesia Vol 22 No 1 (2025): March
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.22.1.1

Abstract

This study examines the potential for rearing Apis cerana Fabricius honey bees in Taman Kehati Kiarapayung, West Java. Observations were conducted by installing 39 beehive in various locations and observing the flora that serve as bee food sources. A total of 50 plant species from 24 families were identified as bee food sources. Out of the 39 beehive, only 4 were successfully inhabited by bee colonies, showing a success rate of 10.3%. The observed colony growth ranged from 3 to 9 honey combs per hive. The main challenges in cultivation are the decrease in food availability during the dry season with very low rainfall, and pest disturbances such as wax moths (Galleria mellonella), wasps (Vespa sp.), and ants (Formicidae). The results of this study indicate that A. cerana beekeeping has the potential to be implemented in specific locations with sufficient food availability and effective pest management. This potential can serve as an additional source of income for the local community and support environmental conservation efforts by utilizing biological resources and biodiversity in a non-destructive manner at conservation sites.
Desain primer secara in silico dan optimasi PCR untuk deteksi gen penyandi ovary ecdysteroidogenic hormone (OEH) pada nyamuk Aedes aegypti yang terinfeksi Wolbachia: In silico primer design and PCR optimization for detection of the gene encoding ovary ecdysteroidogenic hormone (OEH) in Aedes aegypti mosquitoes infected with Wolbachia Septiasari, Ni Putu Senshi; Dwijastuti, Ni Made Sri; Handayani, Nyoman Sri; Darmayanti, Putu Diah
Jurnal Entomologi Indonesia Vol 22 No 1 (2025): March
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.22.1.9

Abstract

Female Aedes aegypti (Linnaeus) mosquitoes bearing Wolbachia are reported to have behavioral changes during mating and blood-sucking. The ovary ecdysteroidogenic hormone (OEH) is a hormone released when female mosquitoes consume blood so that it influencing egg formation. Initial studies regarding the genetics of the A. aegypti mosquito carrying Wolbachia are very necessary to able to do this help control dengue vectors. The polymerase chain reaction (PCR) method is an appropriate method for genetic studies. Primer design is an initial and crucial stage in genetic studies because designing specific primers will determine the success of the PCR process. This research includes several stages of in silico primer design to design OEH gene primers and test the optimization of the in vitro designed primers. The in silico stage which involves primer design, primer BLAST, primer quality testing, and in silico PCR simulations. The designed primers were tested in vitro by performing PCR using various variations in primer concentration (0.2 µM and 0.4 µM) and annealing temperature (start from 45 oC to 60 oC). The results obtained in silico were two pairs of primers: primers OEH 8 and OEH 9. The results of the in silico test obtained a DNA product size of 264 bp for Primer OEH 8 and 236 bp for primer OEH 9, and the PCR efficiency was 95%. The results of the in vitro test showed only primer OEH 9 met the primer criteria according to the in silico test: primer concentration of 0.4 µM with an annealing temperature of 57 oC.

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