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Scripta Biologica

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Published by Universitas Jenderal Soedirman

ISSN : - EISSN : 23553138 DOI : -

Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Scripta Biologica is a peer reviewed open access electronic journal published by the Faculty of Biology Jenderal Soedirman University, publishes research papers encompasses all aspects of biology, including; molecular biology, cell biology, and genetics; physiology and reproduction; ecology and conservation; biodiversity, evolution, taxonomy, and biogeography.

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Articles 13 Documents

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Search results for , issue "Vol 4, No 3 (2017)" : 13 Documents clear

ISOLASI BAKTERI ASAM LAKTAT DARI KIMCHI DAN KEMAMPUANNYA MENGHASILKAN ZAT ANTI BAKTERI Benedicta Yolanda Khristnaviera; Vincentia Irene Meitiniarti
Scripta Biologica Vol 4, No 3 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

South Korea is famous for its fermented food called Kimchi, a traditional Korean food fermented from pickled vegetables with a mixture of spicy seasoning. Kimchi is now one of functional food products because of there are lactic acid bacteria that are probiotic and can produce bacteriocin compounds. These bacteriocin compounds may inhibit or have anti-bacterial activity. The purpose of this study was to obtain isolates of lactic acid bacteria from store-bought kimchi and homemade kimchi, to examine the antibacterial agent produced by lactic acid bacteria isolated from kimchi against Escherichia coli and Staphylococcus aureus bacteria. We conducted a Disk Diffusion Method to tests the bacteriocin activity, and data were descriptively analyzed. The results showed that eight isolates of lactic acid bacteria from store-bought kimchi and homemade kimchi homemade were able to inhibit the growth of tested bacteria, S. aureus and E. coli. Isolate D1 isolated from store-bought kimchi has largest inhibitory capability against S. aureus and E. coli; it has 16.00 mm and 17.33 mm inhibitory zone, respectively. Isolate B2 isolated from homemade kimchi has the most significant inhibitory ability against S. aureus and E. coli; it has 16.67 mm and 17.67 mm inhibitory zone, respectively. The lowest ability to form clear zone was found on isolate D2 isolated from homemade kimchi. The inhibitory zone of produce by strain D2 against S. aureus and E. coli were 7.67 mm and 8.67 mm, respectively.

PERBEDAAN PROFIL LIPID DAN KADAR ASAM URAT PADA PASIEN DIABETES MELITUS TIPE II DENGAN DAN TANPA HIPERTENSI Filliana Savitri; Intani Kurnia Savitri; Pugud Samodro; Lantip Rujito
Scripta Biologica Vol 4, No 3 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Diabetes Mellitus type 2 is the 7th world of death problem with the 95% proportion rate. Hypertension has also a correlation with this condition. The mortality of cardiovascular is 2-3 times over from diabetics with hypertension than diabetics with normotension which caused by lipid and aciduric disturbations. This study was conducted using cross-sectional design. Twenty-five sample of diabetics normotension and 25 sample of diabetics patient along with hypertension in Ajibarang Hospital District Banyumas and comply with inclusion-exclusion criteria were taken suing consecutive sampling. Data from the cholesterol and triglyceride total test were collected and analyzed with Mann-Whitney test. Additionally, HDL cholesterol and LDL was analyzed using T-Test. This Study showed that average total cholesterol from diabetics along with hypertension patients was in a range of 185.16 ± 36.59 and Diabetics patient without hypertension was 196.76 ± 44.62 (p = 0,503). Besides, the average rate of triglyceride level on diabetics patient with hypertension was 181.76 ±72.68 and diabetics patient along without hypertension was 180.28 ± 125.75 (p=0,367). The average rate of HDL cholesterol level on diabetics patient with hypertension was 39.12 ± 11.07 and diabetics patient along without hypertension was 42.37 ± 14.98 (p = 0,387). In addition, Average rate of LDL level on diabetics patient with hypertension was 112.57 ± 24.77 and diabetics patient along without hypertension was 118.33 ± 37.41 (p = 0,524). The average rate of uric acid on diabetics patient with hypertension was 6,120 ± 1,45 and diabetics patient along without hypertension was 5,768 ± 2,45 (p=0,200). As a conclusion, there is no difference with total cholesterol, triglyceride, HDL, LDL cholesterol, and uric acid significant from diabetics patient with or without hypertension in Ajibarang Hospital District Banyumas in 2016.

PETUNJUK PENULIS dan DAFTAR ISI Romanus Edy Prabowo
Scripta Biologica Vol 4, No 3 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

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AKTIVITAS PROTEASE DAN AMILASE PADA IKAN SIDAT Anguilla bicolor McClelland) Mohamad Taufik; Hana Hana; Untung Susilo
Scripta Biologica Vol 4, No 3 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

This study was experimental, conducted using a Completely Randomized Design (CRD) with 3 x 2 factorial design, and four replicates have been carried out to evaluate the protease and amylase activities of Anguilla bicolor McClelland. A total of 71 individuals divided into three weight groups were used in this study. The first group with an average weight of 41.25 ± 0.898 g consisted of 51 eels, the second with an average weight of 319.8 ± 4.666 g composed of 14 eels, and the third with a mean weight of 569.5 ± 9.150 g consisted of 6 eels. The results showed the protease activity differed significantly based on eel size and intestinal segment (P ˂ 0.05). This research recorded the highest protease activity was in eels within the smallest weight group (41.25 ± 0.898 g). This study also revealed the protease activity in the anterior intestine was higher than the posterior in all size of eels. The amylase activity did not differ significantly (P>0.05) by eel size and intestinal segment. This study concluded the protein digestion capacity of smaller eels was higher than larger eels, and the protein digestion capacity was greater in the anterior intestine than the posterior intestine. The carbohydrate absorption capacity in eel was not affected by the variety of fish size which indicates no change in the feed category.

NEMATODE COMMUNITY RESPONSE TO VARIED PROPORTION OF DECOMPOSING PLANT LITTER Anita Rachman Sholeha; Ardhini Rin Maharning; Erie Kolya Nasution
Scripta Biologica Vol 4, No 3 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

The research objectives were to investigate nematode community response to decomposing plant litter of varying proportions, and to measure the litter decomposition rate. This greenhouse experiments included three treatments of decomposing plant litter with proportions of 30:70, 50:50, and 70:30 (leaf litter to stem-paddy litter, weight to weight), and two controls (commercial organic fertilizers and no-organic matter). We applied the prepared plant litter and organic fertilizers on the surface of the agricultural sandy soil. Soil samples were taken after two, seven, 14, 28, 49, and 77 days to follow nematode temporal changes. Nematodes were assigned to functional groups and family level of a taxon. We used Anova to test the significance of nematode abundance among the treatments, exponential decay model to measure decomposition rates of the plant litter, and canonical correspondence analysis to investigate the correlation of varying plant proportion and nematode community composition. The results showed that nematode functional groups and family did not differ among soils of various plant litter proportions. Bacterivorous Rhabditidae and Cephalobidae, fungivorous Aphelenchidae, and fungivorous-root feeder Tylenchidae inhabited plant litter amended soils. Their abundance, however, differed significantly (p<0.001) and revealed two temporal patterns, i.e., linear (50:50) and unimodal (30:70 and 70:30). No nematodes were observed in organic fertilizer amended soil and no-organic matter soil. Nematode community composition changed over time according to the litter proportion, in which by the end of experiments, community of 50:50 and 30:70 were the least similar (r: 0.57 of axis-1 and 0.54 of axis-2, CPV: 89.06). We concluded that all litter proportions retained similar quality and supported the growth of similar nematode groups. The litter proportion, however, appeared to influence nematode abundance, their temporal patterns, and community compositions.

ANALISIS KONDISI HABITAT DAN PERAKARAN TUMBUHAN BAWAH PADA DAERAH TERBUKA DAN DI BAWAH TEGAKAN CEMARA UDANG DI PESISIR LEMBUPURWO, KEBUMEN Winastuti Dwi Atmanto; Hesti Wulan Ndari; Sri Danarto
Scripta Biologica Vol 4, No 3 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Coastal areas with sandy soil structure without trees have high temperature and salinity, strong wind, as well as minimum nutrient content. The limitation of the environment conditions affects the variety of undergrowth. This research aims to figure out the composition, distribution patterns, habitat conditions and rooting from undergrowth species which are found in coastal sandy areas at Lemburpurwo. The research was conducted using line transect method defined by systematic sampling with random start. The observations of undergrowth were carried out on 3 types of treads near from the sea, under and behind Cemara Udang stands. Paths are made perpendicular within approximately 53 m from the shoreline and the distance between lines is 95 m. Each track was divided into segments in the form of 2x2 m2 plots which were laid out diagonally between plots for undergrowth observation. 81 plots were taken, where the size of the area is 72960 m2. Undergrowth plant species were identified, the length and number of roots were measured, environmental conditions and soil quality including pH, DHL, N total content, P and K available were observed. Lembupurwo coastal sandy area consists of 20 species of 13 plant families with 3 types of random distribution pattern and 17 types of clustered distribution pattern. Spinifex littoralis has the longest root as well as the largest number of roots, especially in open areas. The pH values and electrical conductivity below the stands of 6.41; 32,23 µs/cm are lower when compared to locations near the sea. N-total and P available under stands are available 0,03%; 5,05 ppm which is higher than the other two locations.

PENGARUH AIR REBUSAN DAUN MAMPELAS (Tetracera indica Merr.) TERHADAP KADAR KOLESTEROL DARAH MENCIT Dian Samitra; Zico Fakhrur Rozi
Scripta Biologica Vol 4, No 3 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Tetracera indica Merr. locally known as Mampelas is commonly used in traditional remedies. It believed to have the potential to cure hypercholesterolemia. The potential use of this plant to lower blood cholesterol level was understudied; therefore this study aimed to determine the effect of the infusion of fresh leaves of Tetracera indica to the blood cholesterol levels of mice. This research was true experimental design, a post-test only design with nonequivalent (control) groups. This study used 25 male mice divided into five categories, i.e., P0 mice were administered with 0 g of infusion, P1 mice with 2 g infusion, P2 mice with 5 g infusion, P3 mice with 10 g infusion, and P4 mice without Tetracera indica infusion but 10 mg of simvastatin. The treatment in all categories were given continuously for seven days. The results showed the mean of the blood cholesterol levels were 169.4 ± 59.37 mg/dL in P0, 121.8 ± 7.62 mg/dL in P1, 136.2 ± 15.69 mg/dL in P2, 110.6 ± 37 , 01 mg/dL in P3 , and 179,0 ± 35,24 mg/dL in P4. Kruskal Wallis test was calculated to determine the difference of blood cholesterol level among all groups, and it reported p < 0.05. The results of this study showed that infusion of Tetracera indica leaf was significantly lowered the level of blood cholesterol in mice.

STRUKTUR KOMUNITAS DAN DISTRIBUSI TEMPORAL GASTROPODA DI DANAU SITU GINTUNG, TANGERANG SELATAN, BANTEN Yayan Mardiansyah Assuyuti; Alfan Farhan Rijaluddin; Firdaus Ramadhan; Reza Bayu Zikrillah; Dwi Cahya Kusuma
Scripta Biologica Vol 4, No 3 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

The changes in the physicochemical of waters in the lake ecosystem caused by seasonal variation, anthropogenic and industrial wastes impact the gastropod community. This study aimed to determine the physicochemical parameter of waters, community structure and distribution of gastropod, and the correlation between those two in the dry and rainy seasons. We conducted this research in the lake of Situ Gintung located in Tangerang Selatan, Banten, Indonesia during the dry season (May to August 2015) and the rainy season (February to April 2016). Statistical analysis showed the physicochemical parameter of waters in both seasons has no different, while the gastropod showed a slight difference in occurrence based on the season, in which rainy season had the highest occurrence. Canonical Correspondence Analysis (CCA) suggested that gastropods occurrence were affected by the light intensity in the rainy season and the temperature in the dry season.

PENGARUH PEMBERIAN EKSTRAK KULIT PISANG KEPOK KUNING (Musa balbisiana) TERHADAP ASUPAN MAKAN DAN BERAT BADAN TIKUS WISTAR (Rattus norvegicus) JANTAN Wiwiek Fatchurohmah; Andreanyta Meliala
Scripta Biologica Vol 4, No 3 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

A positive energy imbalance results in overweight and obesity. Overweight and obesity are the major risk factors for many diseases, including diabetes, cardiovascular diseases, and mental health. Animal studies suggested that serotonin involves in food intake control. The Banana peel is a potential natural source of serotonin precursor; furthermore, it may influence food intake. This study aimed to determine the effect of banana peel extract on food intake and body weight gain in male Wistar rats. Twenty-four male Wistar rats (8-10 weeks old), were divided into four groups for feeding treatment. All four group were; the control group (C), the banana peel extract dosage of 4 g/kg BW group (T1), the banana peel extract dosage of 8 g/kg BW (T2), and the banana peel extract dosage of 16 g/kg BW (T3). The food intakes were measured daily, while rats body weights were recorded at the beginning and the end of the three days experimental periods. The study found the rats in the T1 group (8.80 ± 2.63) showed no significant difference in food intake compared to C group (10.68 ± 1.89), T2 group (6.65 ± 3.12), and T3 group (5.42 ± 1.59). Rats in the T2 group (p = 0.038) and T3 group (p = 0.005) showed lower food intake significantly compared to control group. After three days of treatment, the T3 group showed a significant decrease in body weight compared to control group (p = 0.008). This study concluded, the administration of banana peel extract dosage of 8 g/kg BW and 16 g/kg BW suppressed food intake, while the dosage of 16 g/kg BW reduced the body weight in male Wistar rats.

KEANEKARAGAMAN MIKROALGA DI SUNGAI KATI LUBUKLINGGAU Harmoko Harmoko; Sepriyaningsih Sepriyaningsih
Scripta Biologica Vol 4, No 3 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

We conducted an initial survey of the microalgal diversity of Kati River located in the City of Lubuklinggau, South Sumatra Province, on March to May 2017. This study aimed to determine the microalgal diversity of Kati River as a baseline data. This study found 47 microalgal species belonging to 42 genera and 27 orders. We found the member of Chlorophyceae and Bacillariophyceae were the most abundant microalgae. We also compared the diversity of Kati River to two other rivers in Lubuklinggai, Mesat River, and Kalingi River. The microalgal diversity of Kati River is higher than Mesat River but lower if compared to Kalingi River. The physicochemical factors of Kati River waters were also measured, the average water temperature was 27°C, average pH was 6.7, and average water transparency was 57 cm. This study found that those environmental factors influenced microalgal diversity in Lubuklinggau rivers, Kati River, as well as Mesat River and Kalingi Rivers, have indicated the same results.

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