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MOHAMAD FADJAR
Fakultas Perikanan Dan Ilmu Kelautan, Universitas Brawijaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Physics Public Health Social Sciences Veterinary Other

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Metode Sederhana dan Efektif Untuk Penghitungan dan Visualisasi Tiga Dimensi (3D) Biofilm Vibriio Cholera Prihanto, Asep Awaludin; Sukoso, Sukoso; Fadjar, Mohamad; Kurniawan, Andi
Media Penelitian dan Pengembangan Kesehatan Vol 25, No 3 Sep (2015)
Publisher : Badan Penelitian dan Pengembangan Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3588.565 KB)

Abstract

AbstrakMikroorganisme yang mampu menghasilkan biofim menimbulkan masalah yang serius dalam bidang kesehatan dan pangan. Penelitian biofim bagi sebagian peneliti sangat identik dengan kerumitan proses penghitungan dan visualisasi penutupan permukaan substrat penempelan bakteri. Penelitian ini ditujukan untuk mengetahui efiiensi metode alternatif untuk menghitung dan memvisualisasikan biofimVibrio cholera. Pada penelitian ini beberapa faktor lingkungan seperti pH, suhu, dan kondisi kultur diujicobakan untuk mengetahui pengaruhnya terhadap pembentukan biofim V. cholera. Pembentukan biofim dihitung berdasarkan Biofim Coverage Rate (BCR) yang selanjutnya divisualisasikan menjadi bentuk tiga dimensi (3D) dengan memanfaatkan software Image-J. Hasil penelitian menunjukkan bahwa pH, suhu, dan kondisi kultur mampu memberikan pengaruh yang signifian terhadap pembentukan biofim Vibrio cholera. Metode alternatif yang digunakan dalam penelitian ini mampu menghitung BCR serta menggambarkannya dalam bentuk 3D dengan efiien sehingga dapat dijadikan alternatif analisis biofim bakteri. AbstractMicroorganism which produces biofim, will causes serious issues in health and safety of food. Researches in biofim are identic with the complexities and relatively laborious tasks especially on assaying and visualizing method of biofim. This study was aimed to calculate and visualize biofims produced by Vibrio cholera. In this study several environmental factors such as pH, temperature, andculture conditions have been tested to determine its inflence on biofim formation of Vibrio cholerae.Biofim formations were calculated and visualized based on Biofim coverage rate (BCR) and threedimensions (3D) structure using Image-J software. The results showed that pH, temperature, and cultureconditions had a signifiant inflence on the formation of V. cholerae biofim. The alternative method thatwas used in this study could effiiently calculate and visualize BCR to 3D structures. Therefore, here wereport an alternative method for calculating bacterial biofim.
Pengaruh Perbedaan Sistem Budidaya terhadap Pola Pita Protein Daging Udang Vaname (Litopenaeus vannamei) Martini, Ni Nyoman Dian; Nursyam, Happy; Fadjar, Mohamad
Prosiding Seminar Nasional MIPA 2015: PROSIDING SEMINAR NASIONAL MIPA UNDIKSHA 2015
Publisher : Prosiding Seminar Nasional MIPA

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Abstract

Penerapan teknologi bioflok, sistem budidaya heterotrofik dengan pertukaran air minimal, saat ini sedang dikembangkan dalam kegiatan budidaya udang vaname (Litopenaeus vannamei) karena dapat memberikan produktivitas yang lebih tinggi, konversi pakan yang lebih rendah, dan kualitas air yang lebih stabil. Pada teknologi bioflok, bakteri heterotrofik di dalam sistem budidaya cenderung membentuk agregat (bioflok) yang dapat dikonsumsi oleh udang sebagai sumber protein alami. Dalam penelitian ini, studi SDS-PAGE dilakukan pada daging L. vannamei sepanjang siklus produksi tambak intensif dengan sistem budidaya yang berbeda (Fitoplankton, Semi-Bioflok, dan Bioflok) untuk menyelidiki pengaruh sistem budidaya yang berbeda tersebut terhadap pola pita protein daging L. vannamei. Penelitian ini berlangsung pada beberapa lokasi di Kabupaten Tuban, Jawa Timur. Tujuan penelitian ini adalah untuk memperoleh karakteristik pita protein daging L. vannamei yang berasal dari sistem pemeliharaan yang berbeda. Hasil penelitian menunjukkan bahwa sistem bioflok menghasilkan pola pita protein dengan variabilitas yang lebih tinggi karena sistem ini dapat mempertahankan kualitas air yang baik selama pemeliharaan dan menyediakan sumber protein alternatif berupa bioflok bagi L. vannamei selain pakan pelet.Kata kunci: udang vaname (L. vannamei), sistem bioflok, daging, pita protein, SDS-PAGEAbstractApplication of biofloc system, a heterotrophic culture with minimal water exchange, has gained interest particularly in relation to providing high productivity, low feed-conversion ratios, and a stable culture environment, especially in shrimp. In biofloc system, heterotrophic bacterial tends to form noticeable aggregates (biofloc), which can be consumed by shrimp as a natural protein source. In relation to this, the impact of different culture systems on white shrimp (L. vannamei) muscle protein patterns was investigated. In this research, an SDS-PAGE study was performed on white shrimp muscle tissue along the production cycle in intensive shrimp culture ponds with different culture systems (Phytoplankton, Semi-Biofloc, and Biofloc). The study was located in different areas of the District of Tuban, East Java. The aim of this study was to accomplish systematic characterization of the white shrimp muscle protein pattern, which derives from each of the ponds with different rearing systems. The result of the study showed that the biofloc system yields variability of protein pattern as it maintains good water quality throughout the culture and provides an alternative protein source (as biofloc) for the shrimp besides the pellet.Key words: white shrimp (L. vannamei), biofloc system, muscle, protein pattern, SDS-PAGE
Model Simulasi Panen Parsial Pada Pengelolaan Budidaya Intensif Udang Vannamei (Litopenaeus vannamei) Abdul Wafi; Heri Ariadi; Mohamad Fadjar; Mohammad Mahmudi; Supriatna Supriatna
Samakia : Jurnal Ilmu Perikanan Vol 11 No 2 (2020): Samakia: Jurnal Ilmu Perikanan
Publisher : Faculty of Science and Technology University Ibrahimy

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (576.626 KB) | DOI: 10.35316/jsapi.v11i2.928

Abstract

Panen parsial adalah metode panen sebagian biomassa udang pada saat kegiatan operasional budidaya sedang berlangsung. Tujuan dari penelitian ini adalah menentukan konseptual model optimalisasi pengelolaan budidaya udang vannamei (L. vannamei) pola intensif berdasarkan prosentase estimasi panen parsial. Penelitian ini dilaksanakan dengan konsep desain kausal ex-post facto selama satu siklus budidaya intensif udang vannamei (L. vannamei). Hasil dari penelitian menunjukkan, selama masa budidaya parameter kualitas air yang meliputi pH, suhu, oksigen terlarut, nitrit, dan nitrat masih berada dalam ambang batas, sedangkan posfat, TAN, dan TOM berada diatas level ambang batas baku mutu untuk budidaya udang intensif. Untuk konsep model simulasi pengelolaan budidaya intensif dari skenario panen non-parsial, parsial 10%, dan parsial 20%. Sistem panen parsial 10% memiliki beberapa keunggulan dibandingkan dua sistem panen lainnya. Yakni, dengan diterapkannya simulasi panen parsial 10% didapatkan nilai efisiensi biomassa panen sebesar 88.08 kg dan efisiensi prosentase survival rate udang sebesar 11% dibandingkan dengan konsep panen parsial 20% atau non-parsial. Sehingga dapat disimpulkan, bahwa untuk konsep model pengelolaan budidaya udang vannamei (L. vannamei) pola intensif yang paling optimal berdasarkan hasil simulasi sistem pemodelan dinamis adalah konsep model budidaya panen parsial 10% dengan pelaksanaan panen parsial pada saat budidaya umur 60 hari.
PROGRAM PEMBERDAYAAN TIM PENGGERAK PKK DESA AMPELDENTO, KARANGPLOSO, MALANG MELALUI KEGIATAN PENGEMBANGAN SISTEM AKUAPONIK Asus Maizar Suryanto Hertika; Mohamad Fadjar; Setya Widi Ayuning Permanasari; Bimo Aji Nugroho
Riau Journal of Empowerment Vol 4 No 2 (2021)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/raje.4.2.83-94

Abstract

PKK (Family Welfare Empowerment) group in Ampeldento Village is a community group whose role needs to be increased, especially in improving family welfare. The problem they have is that the PKK group does not understand the potential of the narrow land owned by the family is the potential to increase family nutrition through the development of aquaponic cultivation systems. Therefore, carried out the implementation of community service programs through the development of the aquaponics system. Data collection techniques are done through interviews and questionnaires. Evaluation of community understanding of the program was carried out using pre-test and post-test. Based on the results of questionnaires and interviews before the program's implementation, it was found that 70% of respondents did not know aquaponics, 50% were not interested, 35% were hesitant in developing aquaponics independently and 45% doubted aquaponics could improve people's welfare. Results improved significantly after the program's implementation and were inversely proportional, with 95% of the community believing that aquaponics could improve welfare and 70% of respondents wanted to develop it independently. Respondents stated that vegetables produced are faster growing, tasty, green, nutritious and large.
Budidaya Benih Lobster Pasir (Panulirus Homarus) Dengan Resirculation Aquaculture System (Ras) Di Pokdakan “Pesona Bahari”, Grand Watudodol, Banyuwangi Attabik Mukhammad Amrillah; Mohamad Fadjar; Sri Andayani; Dwi Retno Andriani; I Gede Eko Putra Sri Sentanu; Attabik M. Amrillah; Diana Aisyah
Journal of Innovation and Applied Technology Vol 8, No 1 (2022)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiat.2022.008.01.6

Abstract

Puerulus or lobster seed (Panulirus homarus) often found in Banyuwangi teritory, in line with government regulations that prohibit to export lobster seeds, Lobster seed trading with seed size segmentation can be an alternative to improve the economy of people who are running the Covid19 pandemic. Seed size segmentation is needed because lobster cultivation as a consumption measure takes a long time. One of the efforts made in its cultivation in order to obtain growth rate and survival rate is Recirculation Aquaculture System (RAS). The aim of this activity is to produce “cricket” size seeds raised by RAS. Cultivation activities was carried out 2 months with different types of feed.  Parameters measured every two weeks are growth and survival rate. Water quality measured every day were temperature, DO, pH and salinity, while ammonia, nitrite, and nitrate were measured every two weeks. The results obtained was 100% survival rate.
POTENSI TINTA GURITA (OCTOPUS SP.) SEBAGAI IMUNOSTIMULAN PADA UDANG VANAME (LITOPENAEUS VANNAMEI) RANGGA IDRIS AFFANDI; MOHAMAD FADJAR; NURI MUAHIDDAH; BAGUS DWI HARI SETYONO
GANEC SWARA Vol 17, No 1 (2023): Maret 2023
Publisher : Universitas Mahasaraswati K. Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35327/gara.v17i1.403

Abstract

Vaname shrimp (Litopenaeus vannamei) is an export commodity from the fisheries sub-sector which has high economic value. The development of aquaculture systems from traditional to intensive has the potential for disease attacks. Control of the spread of the disease must be done as early as possible, one method of prevention is using immunostimulants. An alternative source of immunostimulants that can be used to increase the immune system of shrimp is octopus (Octopus sp.) ink. Octopus ink is generally not used or thrown away when the octopus meat is processed. Research on octopus ink is also minimal compared to squid ink and cuttlefish ink. The purpose of this review is to provide an overview of the potential of octopus ink as an immunostimulant for vaname shrimp. It is known that the content of octopus ink consists mostly of alkaloids, melanin, amino acids, and carboxylic acids. Octopus ink has various roles based on its compound content such as antimicrobial, antioxidant, antibacterial, antiretroviral, anticancer, anti-ulcerogenic, anti-inflammatory, antivirus, antifungal, antiviral, and anti-proliferative. From the results of the literature study, it is explicitly necessary to carry out further research to find out more complete compounds content in octopus ink so that its potential as an immunostimulant in vaname shrimp cultivation can be identified more clearly.
GENOTOXIC EFFECT ON HEMATOLOGICAL AND MICRONUCLEUS ALTERATION OF COMMON CARP (Cyprinus carpio L.) EXPOSED BY GLYPHOSATE-BASED HERBICIDE R Adharyan Islamy; Abdul Rahem Faqih; Yuni Kilawati; Yunita Maimunah; Mohamad Fadjar; Veryl Hasan; Wahyu Isroni; Novalina Serdiati; Ayu Winna Ramadhani; Diana Aisyah
Jurnal Perikanan Pantura (JPP) Vol 6 No 1 (2023): MARET 2023
Publisher : Universitas Muhammadiyah Gresik

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30587/jpp.v6i1.944

Abstract

Study aims to analyze the genotoxic potential of glyphosate-based herbicide on common carp (Cyprinus carpio L) using Micronucleus and hematological assay. The concentration of Glyphosate-based herbicide in this study was 0 ppm, 1.35 ppm, 1.8 ppm, 2.4 ppm, 3.2 ppm, 4.2 ppm, 6.5 ppm, and 8.7 ppm. Administration of herbicide base on modification of published methods with 96 hours of exposure. This research result has shown that the average number of micronuclei was increased simultaneously with increasing the concentration of herbicide exposure. There are also other types of cell nucleus abnormalities, namely: blebbed, lobed, notched, and binuclear. In the treatment of 0 ppm of herbicide shown blebbed nuclei are 8 ‰, lobed nuclei are 6.6 ‰, notched nuclei are 10 ‰, binuclei is 4 ‰. From the research results, it can be concluded that the LC50-96 hours exposure of the herbicide isopropylamine glyphosate in carp (Cyprinus carpio L.) was obtained at a concentration of 8.57 ppm. Based on the evaluation on hematology, it was found that there was a decrease in the number of erythrocytes, hematocrit, and hemoglobin of fish blood, whereas the number of leukocytes, micronuclei, and other abnormal micronuclei showed an increase along with the increase in the dose of exposure to the herbicide isopropylamine glyphosate, which indicates a genotoxic effect.
Aplikasi Teknologi Underwater Lobster Apartment Untuk Pembesaran Lobster Pasir (P. homarus) pada Kelompok Pembudidaya Ikan (POKDAKAN) Pesona Bahari, Banyuwangi, Jawa Timur Amrillah, Attabik Mukhammad; Sri Sentanu, I Gede Eko Putra; Andriani, Dwi Retno; Fadjar, Mohamad
Journal of Innovation and Applied Technology Vol 9, No 1 (2023)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiat.2023.009.01.5

Abstract

Kelanjutan Doktor Mengabdi 2021 di POKDAKAN Pesona Bahari, solusi yang ditawarkan adalah dilakukan desain wadah budidaya dengan pemberian sekat antar individu lobster yang dibudidayakan dengan Underwater Lobster Appartment. Teknologi terbaru pada budidaya pembesaran lobster. Lobster yang didapatkan dibesarkan dengan padat tebar 1 ekor lobster per blok apartemen. Satu blok apartemen memiliki dimensi (PxLxT: 30cm x 15cm x 15cm). Pembesaran dilaksanakan dalam kurun waktu 30 hari dengan pakan kerang hijau secara rutin oleh anggota POKDAKAN Pesona Bahari. Kontroling, pengawasan serta pendataan dilaksanakan secara bersama – sama dengan tim DM Universitas Brawijaya dan mahasiswa yang terlibat. Berdasarkan hasil pengamatan dapat disimpulkan bahwa penggunaan system underwater lobster apartemen pada budidaya lobster ukuran sangat berpengaruh positif terhadap pertumbuhan dan kelulushidupan mencapai 100%. Kualitas air dengan system underwater lobster apartemen tetap terjaga dan tidak melebihi nilai kelayakan kualitas air  budidaya.
Phytoplankton Composition in White Shrimp (Litopenaeus vannamei) Pond Culture Infected White Feces Disease (WFD) Anjaini, Jefri; Simangunsong, Tohap; Fadjar, Mohamad
Research Journal of Life Science Vol 10, No 1 (2023)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2023.010.01.4

Abstract

The aim of this study is to find out the composition and condition of phytoplankton in the white shrimp (L. vannamei) culture inffected by the white feces disease so that one of the indicators of the onset of the white feces disease can be known. This research uses descriptive methods. The observed parameters are the identification of species diversity, abundance, diversity index and dominance index of phytoplankton. The sampling was conducted at three locations, Tuban area, Situbondo area, Lamongan area. The results of the study showed observations in Lamongan area, the total abundance of phytoplankton was 473 cells/L. Situpondo areas abundantity of phytoplankton total was 633 cels/L and Tuban areas abundance was 887 cells/L. In Lamongan area, the diversity index was 1,009. In Situbondo areas diversity Index was 1,013. In Tuban area, diversity indexes were 1,082. In Lamongan area, a dominance index was 0.477. In Situpond area, it was obtained a domination index of 0.544. The genus that dominates the three ponds are Cyclotella and Navicula. At all three locations have high variety of species and abundance of the genus members of the filum Cyanophyta and Bacillariofhyta are detrimental, the genuses that are found are indicators of contaminated waters. The dominance of Cyanophyta (blue-green algae) is also an indicator of the White Feces Disease.
Crustacean Hyperglycemic Hormone and Gonad Histology of Female Spiny Lobster (Panulirus homarus) Inducted by Laserpuncture Jonathan Christian Wibowo; Mohamad Fadjar; Yunita Maimunah
Jurnal Penelitian Pendidikan IPA Vol 10 No 11 (2024): November
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i11.7926

Abstract

Spiny lobster is one of the potential commodities in Indonesia. On the other hand, there is still a problem to fulfill the export-ready stock of spiny lobster. Production engineering can be utilized as one way to overcome the existing problems. Laserpuncture is a new method of production engineering by utilizing low wavelength helium-neon light that can provide biological stimulation to living things resulting in accelerated development. Laserpuncture induction in crustaceans such as lobsters can be done on the eye stalk and ventral part. This study was conducted using completely randomized design method with 4 treatments and 5 repetitions of each treatment. The treatments given are differences in the duration of laserpuncture induction on female spiny lobster including A (2 seconds induction), B (4 seconds induction), C (6 seconds induction), and control (without induction). This study aims to analyze wheter laserpuncture affects Crustacean Hyperglycemic Hormone. CHH and gonad in female spiny lobster. Data analysis in this study was carried out qualitatively which included Crustacean Hyperglycemic Hormone A & B hormone, gonad histology, and survival rate. The results showed that treatment A (2 seconds induction of laserpuncture) on female spinny lobster had the most significant effect compared to other treatments.
Co-Authors Abdul Rahem Faqih Abdul Wafi Abdul Wafi, Abdul Andi Al Furqan Andi Kurniawan Asep Awaludin Prihanto Asus Maizar Suryanto H Attabik M. Amrillah Attabik Mukhammad Amrillah Ayu Winna Ramadhani BAGUS DWI HARI SETYONO Bimo Aji Nugroho Diana Aisyah Dwi Retno Andriani Dyah Hariani Happy Nursyam Heri Ariadi Herlina, Cucun Isroni, Wahyu Jefri Anjaini Jonathan Christian Wibowo Maftuch Maftuch Mohammad Mahmudi Mr. I Gede Eko Putra Sri Sentanu Ni Nyoman Dian Martini Novalina Serdiati Nuri Muahiddah Pramudia, Zulkisam Purnama, Erlix Rachmad Purnama, Tarsan R Adharyan Islamy Rangga Idris Affandi Rangkuti, Rizky Fadilla Agustin Rizal Rifa'i, Ramanda Ahmad setya widi ayuning permanasari Sri Andayani Sri Sentanu, I Gede Eko Putra Sukoso Sukoso Supriatna Tohap Simangunsong Valina, Rahmi Veryl Hasan Yuni Kilawati Yunita Maimunah