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Jurnal Ilmu Dasar
Published by Universitas Jember
ISSN : 24425613     EISSN : -     DOI : https://doi.org/10.19184/jid.v24i2.36657
Core Subject : Education, Engineering,
Control & Systems Engineering Mathematics
Jurnal ILMU DASAR (JID) is a national peer-reviewed and open access journal that publishes research papers encompasses all aspects of natural sciences including Mathematics, Physics, Chemistry and Biology. JID publishes 2 issues in 1 volume per year. First published, volume 1 issue 1, in January 2000 and avalaible in electronically since 2012 with ISSN 1411-5735 (Print) and avalaible in electronically since 2012 with ISSN 2442-5613 (online). Jurnal ILMU DASAR is accredited SINTA 3 by the Ministry of Education, Culture, Research, and Technology of the Republic of Indonesia (Kemendibukristek) No. 152/E/KPT/2023 (September 25, 2023), Ministry of Research, Technology and Higher Education of the Republic of Indonesia (RISTEKDIKTI), No. 200/M/KPT/2020 (December. 23, 2020). All accepted manuscripts will be published worldwide JID has been indexed in DOAJ, Dimension, OCLC WorldCat, PKP Index, Crossref, Google Scholar, Base, Garuda, and OneSearch. JID have been collaborated in KOBI-ID (Konsorsium Biologi Indonesia) and HKI (Himpunan Kimia Indonesia) since 2017.
Arjuna Subject : Matematika - Kontrol dan Optimasi
Articles 13 Documents
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Search results for , issue "Vol 12 No 2 (2011)" : 13 Documents clear
A Comparison Study for Agrobacterium-mediated transformation Method in Sugarcane (Saccharum spp L.) Bambang Sugiharto; Hilda Safitri
Jurnal ILMU DASAR Vol 12 No 2 (2011)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

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Abstract

In order to compare transient expression of gus gene driven by CaMV 35S or rice ubiquitin RUBQ2 promoter, Agrobacterium-mediated transformation was conducted using embryogenic callus and suspension cultures of sugarcane. Histochemical observation of GUS activity after co-cultivation showed that rice ubiquitin promoter produced high level of clear blue spots both in embryogenic callus and suspension cultures, while the CaMV35S promoter was not detected. Regenerated shoots from the infected materials were found higher in suspension cultures than embryogenic callus. The results showed that the rice ubiquitin promoter as well as suspension cultures are more efficient for the Agrobacterium-mediated transformation. However, PCR analysis found there was no amplification of DNA neither for CaMV35 nor rice ubiquitin in genome DNA of regenerated shoot. Lacking of integration of the DNA into the genome showed that the regenerated shoots were not truly transformants might due to the presence of somaclonal variation that was common phenomenon in the regeneration from callus. To avoid the presence of somaclonal variation the transformation was then conduced using in vitro plant without intervening callus phase. Basal segment of in vitro plants prepared from axillary buds of sugarcane were used as the explants for the transformation. The histochemical observation of GUS activity showed that almost all of the infected materials partially exhibited blue color on the basal region. Infected in vitro plants showed rapidly grow and multiplied in theselection medium. Further investigation of the transformation using in vitro plants and Agrobacterium harboring SoSUT1 DNA construct driven by rice actin promoter resulted in development of antibiotic resistant sugarcane shoots. Interestingly the PCR analysis found an insertion of the antibiotic DNA fragment into the sugarcane genome DNA. This result suggests that in vitro plant is an effective target tissue for Agrobacterium-mediated transformation in sugarcane.
Characterization of Nata De Coco-Benedict Film by Adsorption as Sensor for Glucose in Urine Sample Dwi Indarti; A Asnawati
Jurnal ILMU DASAR Vol 12 No 2 (2011)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

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Abstract

Research on nata de coco utilization as a film to immobilize benedict reagent for glucose analysis has been conducted. Immobilization of benedict by nata de coco film was evaluated by using adsorption method and characterization i.e. physical characterization (morphology) and viability characterization of its sensors activity for glucose. Morphological characterization (SEM) showed that the benedict reagent has been absorbed by nata de coco film despite leaching activity of 14.7%, with optimum concentration of Benedict of 0.2682 M, 40 min dyeing time at maximum of 541 nm. Sensor characterization of cellulose nata-Benedict for glucose showed regression coefficient of 0.9895, the detection limit of 780 ppm with a sensitivity of 0.001 and adsorption reproducibility of 0.2013%. Glucose content measurement on urine sample by using Nata De Coco-Benedict based Film Nelson-Somogyi.
On Total Vertex Irregularity Strength of Cocktail Party Graph Kristiana Wijaya; S Slamin; Mirka Miller
Jurnal ILMU DASAR Vol 12 No 2 (2011)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

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Abstract

A vertex irregular total k-labeling of a graph G is a function λ from both the vertex and the edge sets to {1,2,3,,k} such that for every pair of distinct vertices u and x, λ(u)+∑λ(uv)≠λ(x)+∑λ(xy). uv∈E xy∈E. The integer k is called the total vertex irregularity strength, denoted by tvs (G ) , is the minimum value of the largest label over all such irregular assignments. In this paper, we prove that the total vertex irregularity strength of the Cocktail Party graph H2,n ,that is tvs(H2,n )= 3 for n ≥ 3.
Phenotypic and genotypic identification of lactic acid bacteria isolated from spontaneous fermentation of unripe var. agung semeru banana (Musa paradisiaca formatypica) N Nurhayati; Betty Sri Laksmi Jenie; Harsi D Kusumaningrum; sri widowati
Jurnal ILMU DASAR Vol 12 No 2 (2011)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

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Abstract

Lactic acid bacteria (LAB) can be found on food products such as milk and meat products, cerealia, cassava, fruits or vegetable products. The objectives of this research were to identify phenotypic and genotypic of lactic acid bacteria isolated from spontaneous fermentation of unripe var agung semeru banana (Musa paradisiaca formatypica). Phenotypic identification was based on general morphology, physiological test, API and Biolog system. Genotypic identification used polymerase chain reaction (PCR) method and analyses of 16S rRNA sequence. The result showed that two groups of LAB (FSnh 1 and FSnh A isolate) can use glicerol, D-ribose, D- xylose, D-glucose, D-fructose, D-mannose, methyl α D-gluco pyranoside, N-acetyl glucosamine, esculin ferric citrate, salicin, D-celiobiose, D-saccharose, gentibiose and potassium gluconate as carbon source. Beside that FSnh 1 isolate used D-galactose, L-sorbose, L-rhamnose, and amygdalin, while FSnh A isolate used metil αD- glukopiranosa, arbutin, D-maltose, D-lactose, D-trehalose, D-turanose, and potassium 5-ketogluconate as carbon source. The genotypic identification showed that Lactobacillus sp associated with the spontaneous fermentation of var agung semeru banana were identified as Lactobacillus salivarus and Lactobacillus fructivorans.
Spline Regression Modelling for Health Problem: Study of Knot and Confidence Interval Netty Herawati
Jurnal ILMU DASAR Vol 12 No 2 (2011)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

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Abstract

This article aimed to study about knot and confidence interval for health science using spline nonparameteric regression.The study used simulation and real data. The result showed that numbers of knot should be placed according to the quantil variable in order to get a good estimation of the data function. Inadditon,confidence interval using bayesian and bootstrap method gave no different result for a small sample size whereas for a big sample size bootstrap gave narrower interval.
Triterpenoid Pentacyclic Antimalarial Activity from the Leaves of Erythrina variegata Tati Herlina; Unang Supratman; Anas Urbanas; Supriyatna Sutardjo; Noor Rain Abdullah; Hideo Hayashi
Jurnal ILMU DASAR Vol 12 No 2 (2011)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

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Abstract

In the course of our continuing research for novel antimalarial agent from Indonesian plants, the methanol extract of the Erythrina variegata leaves showed significant antimalarial activity against Plasmodium falciparum strain K1 in vitro with IC50 6.8 μg/mL. The methanol extract was separated by using variety of chromatography techniques. The chemical structure of an antimalarial compound was determined on the basis of spectroscopic evidence and compared to previous data then this compound is identified as a pentacyclic triterpenoid oleane derivative, namely 3,22,23-trihydroxy-oleane-12-ene. The pentacyclic triterpenoid, 3,22,23-trihydroxy-oleane- 12-ene showed antimalarial activity against 3D7 and K1 strains with IC50 4.3 μg/mL and 24 μg/mL, respectively. These results strongly suggested that E. variegata is a promising sources of antimalarial agents.
Study of Dehydration of Ricinoleic of Castor Oil By P2O5 Marham Sitorus; Sanusi Ibrahim; Hazli Nurdin; Djaswir Darwin
Jurnal ILMU DASAR Vol 12 No 2 (2011)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

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Abstract

The aim of this research is to find out the best temperature, time and amount of dehydrator on dehyration of ricinoleic of castor oil by P2O5. Dehydration as means to obtain linoleic and CLA (conjugated linoleic acid) as glyseride forms. Dehydration was carried out using various temperature, time and amount of dehydrator. The reaction medium was maintained under vacuum through by silica gel to reduced water, gentle bubling with nitrogen thorough by Mg to prevent oxidation, and used Zn powder as an antipolymerization agent. Dehydration was followed by GC which the best result was caused the lowest ricinoleic and the highets linoleic and CLA. Identification was done by FTIR, UV and GC- MS and it was compared with the standard. The best dehydration was obtain for 200oC, 3% (w/w) P2O5, and 4 h with a convertion factor (yield) of 97,94%. The composition of best result was: 1.02% (9c – 12c) linoleic, 41.97% (9c/t – 12t/c) linoleic, 19.50% (9c/t-12t/c) CLA, 4.89% (9t – 12t)linoleic,19.79%(9t–11t)CLA and0.94%ricinoleic.TheratioofCLA:linoleicofdehydrated was 0.82 : 1 or 76.18 % compared to the standard CLA with the proportions of 1.45:1.
Effect of MgO Buffer Layer on The Quality of ZnO Films Grown on C-Sapphire by Plasma-assisted MBE Method Agus Setiawan; Takafumi Yao
Jurnal ILMU DASAR Vol 12 No 2 (2011)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

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Abstract

We have investigated the role of low temperature (LT)-MgO buffer layer on the quality of ZnO films grown on c-sapphirebyplasma-assistedmolecularbeamepitaxy(P-MBE)method. EffectofMgObufferthicknessand its annealing were evaluated. We found that surface morphology and crystalline quality of the ZnO layers were improved by controlling of the buffer layers. There is no improvement in morphology and crystalline quality of the ZnO layers if the buffer thickness is less than the critical thickness. The critical thickness is determined to be 1.5 nm. Furthermore, surface, structural, optical, and electrical qualities of the ZnO layers were improved by annealing MgO buffer at high temperature. Dislocation density of the ZnO layer was reduced from 5.3 x109 cm-2 to 1.9 x109 cm-2 by annealing the MgO buffer layer. The results indicate that we can engineer defect in highly mismatched heteroepitaxial using buffer layer.
Modeling Total Maintenance Cost of a Repairable System in Some Bounded Time Interval S Suyono; J.A.M. van der Weide
Jurnal ILMU DASAR Vol 12 No 2 (2011)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

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Abstract

In this paper we model the total maintenance cost of a repairable system where the inter-maintenance times are modeled as discrete-time and continuous-time renewal processes. The maintenance cost is assumed to be a function of the lifetime of the system. We derive the probability distribution, including the mean and the variance, of the total maintenance cost. The results are presented in the form of generating functions and Laplace transforms that in general have to be inverted numerically. Some examples are presented in this paper.
Handling Outlier in Two-Ways Table by Robust Alternating Regression of FANOVA Models: Towards Robust AMMI Models Alfian Futuhul Hadi
Jurnal ILMU DASAR Vol 12 No 2 (2011)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

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Abstract

AMMI (Additive Main Effect Multiplicative Interaction) model for interactions in two-way table provide the major mean for studying stability and adaptability through genotype × environment interaction (GEI), which modeled by full interaction model. Eligibility of AMMI model depends on that assumption of normally independentdistributederrorwithaconstantvariance. Nowadays,AMMImodelshavebeendevelopedforany conditionofMETdatawhich violencethenormality,homogeneityassumpion. Wecanmentioninthisclassof modelling as M-AMMI for mixed AMMI models, G-AMMI for generalized AMMI models. The G-AMMI was handling non-normality i.e categorical response variables using an algorithm of alternating regression. While in handling the non-homogeneity in mix-models sense, one may use a model called factor analytic multiplicative. The development of AMMI models is also to handle any outlier that might be found coincides withnon-homogeneityconditionofthedata. Inthispaper,wewillpresentofhandlingoutlierinmultplicative model by robust approach of alternating regression algorithm.

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