Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML)
Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) is a journal published by “Association of Clinical Pathologist” professional association. This journal displays articles in the Clinical Pathology and Medical Laboratory scope. Clinical Pathology has a couple of subdivisions, namely: Clinical Chemistry, Hematology, Immunology and Serology, Microbiology and Infectious Disease, Hepatology, Cardiovascular, Endocrinology, Blood Transfusion, Nephrology, and Molecular Biology. Scientific articles of these topics, mainly emphasize on the laboratory examinations, pathophysiology, and pathogenesis in a disease.
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<![CDATA[PROTEIN REKOMBINAN 38 KDA MYCOBAKTERIUM TUBERKULOSIS DAPAT MENGIMBAS PEMBUATAN INTERLEUKIN-2 DAN INTERFERON-γ LIMFOSIT T DI KULTUR SEL MONONUKLEAR DARAH TEPI ]]>
<![CDATA[Maimun Z Arthamin]]>;
<![CDATA[Singgih Pujo Wahono]]>;
<![CDATA[Antiek Primardianti]]>;
<![CDATA[Ati Rastini]]>;
<![CDATA[Tri Wahju Astuti]]>;
<![CDATA[Tri Yudani Mardining Raras]]>;
<![CDATA[Francisca S Tanoerahardjo]]>
<![CDATA[ INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 22, No 2 (2016) ]]>
Publisher : <![CDATA[Indonesian Association of Clinical Pathologist and Medical laboratory ]]>
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DOI: 10.24293/ijcpml.v22i2.1119
<![CDATA[Tuberculosis (TB) is caused by Mycobacterium tuberculosis (M.tb) and is one of the significant mortality causes WHO (2012). Theprimary immune response in TB pathogenesis is Cell Mediated Immunity (CMI), roled by T lymphocytes. Interleukin-2 (IL-2) is a growthfactor for T lymphocytes. Gamma Interferon is the key cytokine in M.tb infection control, synthezised by T lymphocytes. An effectivevaccination strategy is achieved by giving vaccine which is able to stimulate T lymphocytes in synthezising cytokines. The 38 kDa M.tbprotein is potential in the vaccine development program, because it has specific epitopes for T lymphocytes. The aim of this study was toknow how to determine that the 38 kDa recombinant protein of M.tb Malang strain could induce cellular immune response by IL-2 andIFN-γ synthezised by T lymphocytes. The study was carried out by an experimental in vitro study on PBMC from healthy endemic subjects,those having TB contact, and the TB patients themselves. PBMC from subjects was cultured with 38 kDa recombinant protein of M.tbMalang strain, with PPD and without any protein. The analysis of IL-2 and IFN-γ used flowcytometry. The result showed that the highestpercentage of IL-2 was found in the culture with 38 kDa recombinant protein of M.tb Malang strain, in healthy endemic (p=0.000)and in those who had TB contact (p=0.000). the highest percentage of IFN-γ was found in the culture with 38 kDa recombinant proteinof M.tb Malang strain, in healthy endemic (p=0.007) and those who had TB contact (p = 0.105). The 38 kDa recombinant proteinof M.tb Malang strain was able to induce IL-2 and IFN-γ synthezised by TCD3+ lymphocytes from healthy endemic subjects and thosewho had TB contact.]]>
<![CDATA[CD4+ DAN CD8+ INTERFERON GAMMA TUBERKULOSIS PARU AKTIF DAN TUBERKULOSIS LATEN ]]>
<![CDATA[Betty Agustina Tambunan]]>;
<![CDATA[John Wiwin]]>;
<![CDATA[Jusak Nugraha]]>;
<![CDATA[Soedarsono Soedarsono]]>
<![CDATA[ INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 22, No 2 (2016) ]]>
Publisher : <![CDATA[Indonesian Association of Clinical Pathologist and Medical laboratory ]]>
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DOI: 10.24293/ijcpml.v22i2.1116
<![CDATA[Tuberculosis (TB) is a global health problem. Immune response through CD4+ T cells and CD8+T cells is needed to produce Interferongamma (IFN-γ). IFN-gamma is a cytokine that can kill Mycobacterium tuberculosis. Not all individuals will lead to illness or activediseases. The aim of this study was to know the cellular immune response like IFN-gamma expression of T-CD4+ cells and CD8+ cellsbetween active TB with latent TB. The design of the study was cross sectional obervational in a population suffering from active andlatent TB. The subjects consisted of 11 (eleven) active TB patients and 10 (ten) latent TB patients from the Special Pulmonary Hospitaland the Dr Soetomo Hospital, Surabaya. The examination of interferon gamma expression of CD4+ and CD8+ was by Flowcytometrymethod. These results were analyzed by Student t test or Mann-Whitney test. The mean CD4+ percentage of active TB (28.75%) waslower than the latent one (TB) (33.21%) but no significant difference (P value=0.114) was shown. The mean CD8+ percentage ofactive TB (30.46%) was higher than the latent one (TB) (28.87%) but no significant difference (P value=0.481) was found. Themean CD4+IFN-γ percentage of active TB (2.51%) was higher than latent one (TB) (1.10%) and there was a significant difference(P value=0.014). The mean CD8+IFN-γ percentage of active TB (2.91%) was lower than latent one (TB) (4.41%) and there was asignificant difference (P value=0.006). Based on this study, it can be concluded that the mean CD4+IFN-γ percentage of active TB washigher than latent TB and there was a significant difference. The mean of CD8+IFN-γ percentage of latent TB was higher than the activeone (TB). This suggested that CD8+ has a dominant part in latent TB and may be caused by the role of other cytokines, or genetics,nutrition, and body mass index factors.]]>
<![CDATA[ANTI-HIV DAN SUBTIPE HIV PADA PASIEN HEMODIALISIS ]]>
<![CDATA[Retno Handajani]]>;
<![CDATA[Mochammad Thaha]]>;
<![CDATA[Mochamad Amin]]>;
<![CDATA[Citrawati Dyah Kencono Wungu]]>;
<![CDATA[Edhi Rianto]]>;
<![CDATA[Pranawa Pranawa]]>
<![CDATA[ INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 22, No 2 (2016) ]]>
Publisher : <![CDATA[Indonesian Association of Clinical Pathologist and Medical laboratory ]]>
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DOI: 10.24293/ijcpml.v22i2.1124
<![CDATA[Anti-Human Immunodeficiency Virus (Anti-HIV) was performed from 100 plasma Chronic Kidney Disease (CKD) stage 5 patientswith continuous hemodialysis (HD) at the Hemodialysis Instalation Dr Soetomo hospital, Surabaya, Indonesia, using three (3) kind ofreagents: Tri-line HIV Rapid test Device from Acon for HIV 1/2/O as strips form, Foresight HIV 1/2/O Antibody EIA Test Kit from Aconand Anti-HIV 1+2/Subtype O ELISA from Axiom. HIV RNA and HIV subtype were detected by Reverse Transcription Polymerase ChainReaction (RT-PCR) based on HIV gag region and analysis of DNA result. Seventy three % patients were hemodialysed twice in a week andonly 14% with duration more than five (5) years. Most of the patients (43%) were hemodialysed between 100−300 times. From the 100plasma samples was obtained only one (1%) man patient plasma sample with positive anti-HIV. A weak positive of RT-PCR result wasnot succeed to be sequenced for determining the HIV subtype. This cause was suspected due to low levels of HIV RNA in blood. The resultsof this study was expected can be used as an additional management consideration of hemodialysis patients at the Hemodialysis Unit.]]>
<![CDATA[KADAR SURFACTANT PROTEIN-D SERUM PADA PASIEN PENYAKIT PARU OBSTRUKTIF KRONIS BERKEBAHAYAAN KAMBUHAN RENDAH DAN TINGGI ]]>
<![CDATA[Dewi Nurhayati]]>;
<![CDATA[Ida Parwati]]>;
<![CDATA[Tiene Rostini]]>;
<![CDATA[Arto Yuwono]]>
<![CDATA[ INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 22, No 2 (2016) ]]>
Publisher : <![CDATA[Indonesian Association of Clinical Pathologist and Medical laboratory ]]>
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DOI: 10.24293/ijcpml.v22i2.1122
<![CDATA[Chronic obstructive pulmonary disease (COPD) cause high morbidity and mortality worldwide. The exacerbations in chronicobstructive pulmonary disease accelerate the decline in lung function and health status, as well as in increasing the treatment cost andmortality risk. The spiro metric measurement has several limitations in assessing the severity as well as the exacerbation risk in COPDpatients. Currently, has been available serum surfactant protein-D, a marker of lung inflammation and lung tissue damage. This proteinis produced by the alveolar type II cells and the Clara cells that play role in maintaining the lung stability and pulmonary immunesystem. The increased level of serum SPD indicates that there is lung epitihelial leakage in line with COPD severity increment and reflectedin COPD exacerbation level of risk according to combined COPD assessment GOLD criteria of the year 2011. The aim of this study wasto know the differences of SP-D serum levels between low and high risk of exacerbation in COPD patients by determination them. Thisstudy was conducted from March to July 2014. The subjects of this study were COPD patients diagnosed by spiro metric measurement.The research was conducted in comparative analytic way with a cross sectional study design. The statistical analysis was performedusing Mann-Whitney non-parametric test. The subjects were 62 COPD patients. The SP-D serum level at low risk of exacerbation groupdiffer significantly compared to the high risk exacerbation group, 1.8–68.4 ng/mL and 3.36–116.4 ng/mL respectively (P=0.018).Based on this study it can be concluded that the SP-D serum levels were higher in COPD patients with high risk exacerbation than thelower risk one. The SP-D serum levels may be considered as a specific marker of lung tissue injury to assess the risk of the exacerbationin COPD patients.]]>
<![CDATA[PENILAIAN UJI TROPONIN I DENGAN POINT OF CARE TESTING ]]>
<![CDATA[Sheila Febriana]]>;
<![CDATA[Asvin Nurulita]]>;
<![CDATA[Uleng Bahrun]]>
<![CDATA[ INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 22, No 2 (2016) ]]>
Publisher : <![CDATA[Indonesian Association of Clinical Pathologist and Medical laboratory ]]>
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DOI: 10.24293/ijcpml.v22i2.1112
<![CDATA[Troponin I is a cardiac biomarker recomended by The Third Global Myocardial Infarction Task Force World Health Organisation(WHO). Troponin plays a central role as a relevant biomarker that require reliable samples, methods, device and efficiency of time.Selecting the device, methods and sample used in the assay may affect the results and turn arround time. The aim of this study is toknow troponin I result using Point of care Testing device with a flourescence immunoassay methods using whole blood and laboratorybasedanalysis device with Enzyme-Linked Fluorescent Assay (ELFA) methods using serum by evaluation. Cross sectional study was heldon 50 subjects in Wahidin Sudirohusodo hospital during the period between July-August 2015, those who suspected suffering acutecoronary syndome (ACS) and underwent troponin I test ordered by the physician and also had whole blood sample. The subjects arearound 51.96±12.80 year old and most of them are men (62%). The mean consentration of troponin I with laboratoric-based analysis is0.50±1.69 μg/L and with POCT is 0.51±1.77. The Pearson correlation test shows the correlation (r) is 0.99 with the p value is <0.001.Bland and Altman methods show the mean difference between two assays is 0.014μg/L (95% confidence interval, -0.015; 0.043) withthe limit of agreement -0.19 to 0.22. Based on this study, it can be concluded that troponin I assay using POCT device can be used tosupport ACS diagnosis precisely and rapidly. It is suggested to perform futher study with concern on the patient’s clinical condition aswell as the diagnosis, so it can evaluate the device performance to measure troponin I levels consistently with the clinical condition.]]>
<![CDATA[PERBANDINGAN NILAI DIAGNOSTIK IGE SPESIFIK TUNGAU DEBU RUMAH, METODE ELISA DAN IMUNOBLOT PADA RINITIS ALERGI ]]>
<![CDATA[Janti Tri Habsari]]>;
<![CDATA[Aryati Aryati]]>;
<![CDATA[Dwi Reno Pawarti]]>
<![CDATA[ INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 22, No 2 (2016) ]]>
Publisher : <![CDATA[Indonesian Association of Clinical Pathologist and Medical laboratory ]]>
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DOI: 10.24293/ijcpml.v22i2.1113
<![CDATA[The detection of allergen types is very helpful in allergic rhinitis (AR) management. Some methods had been performed to examinethe specific IgE due to HDM such as ELISA and immunoblot methods. The aim of this research is to know the difference of specificIgE diagnostic value due to HDM between ELISA and immunoblot in allergic rhinitis method which is expected to be used as in vitroalternative method which is safe, fast, effective, with a high sensitivity and specificity by provement. The samples were allergic rhinitisand non-allergic rhinitis patients at ENT of Head and Neck Out patients Clinic of Dr. Soetomo Hospital Surabaya. The sera was examinedfor specific IgE due to HDM by ELISA and immunoblot methods and then analyzed for its diagnostic value using the 2 x 2 table with a95% confidence interval. The comparation between both methods were analyzed with Wilcoxon test. The diagnostic value of the specificHDM IgE with immunoblot method showed sensitivity of 90% and 80% specificity, positive predictive value 90% and the negative 80%and diagnostic efficiency 86.67%. The positive likelihood ratio 4.5 and the negative one 0.125. The diagnostic value of the specific IgEHDM/D.p with ELISA showed a sensitivity of 75% and specificity 75%, the positive predictive value 85.71% and the negative one 0%and diagnostic efficiency 75%. The positive likelihood ratio was 3 and the negative one 0.33. The diagnostic value of the specific IgEHDM with immunoblot showed a sensitivity of 90% and specificity 80%, the positive predictive value 90% and the negative one 80%and the diagnostic efficiency 86.67%. The positive likelihood ratio was 4.5 and the negative one 0.125. The difference of diagnostic valuein both methods revealed that the p value was 0.013. It can be concluded in this study that there was a significant difference of specificIgE due to HDM between ELISA and immunoblot methods in allergic rhinitis.]]>
<![CDATA[KENASABAN FOSFAT SERUM, C-REAKTIF PROTEIN DAN FETUIN A DI PASIEN GINJAL TAHAP AKHIR DENGAN HEMODIALISIS ]]>
<![CDATA[KS, Indranila]]>;
<![CDATA[Winarto, Heri]]>;
<![CDATA[AP, Purwanto]]>
<![CDATA[ INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 22, No 2 (2016) ]]>
Publisher : <![CDATA[Indonesian Association of Clinical Pathologist and Medical laboratory ]]>
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DOI: 10.24293/ijcpml.v22i2.1125
<![CDATA[Patients with end-stage renal disease (ESRD) who undergo hemodialysis have chronic inflammation caused by several factors,including biocompatibility of dialyzer membran. Inflammation will decrease the fetuin A level in serum. The elevation of the markerinflammation (CRP), hyperphosphatemia and decrease of fetuin A level are the risk faktors for vascular calcification. The aim of thisstudy was to know the relationship between serum phosphate, CRP and fetuin A levels in ESRD patients who underwent hemodialysis. Theresearch was carried out by observational cross sectional analytical approach with consecutive sampling on 31 ESRD patients undergoingchronic hemodialysis in the Hemodialysis Unit of the Dr.Kariadi Hospital, by examining levels of CRP by ELISA, serum phosphate byPhosphomolibdate methods and fetuin A by ELISA. The relationships between phosphate serum and CRP, CRP and fetuin A, phosphateserum and fetuin A were analyzed using Spearman correlation test. In this study it was found that 100% of subjects had CRP levels >0.3mg/dL (reference range 0.1-0.3 mg/dL); 61.3% of subjects had serum phosphate >5.1 mg/dL (reference range 2.5-5.1 mg/dL) and allsubjects (100%) had fetuin A levels <0.5 ng/mL (reference range 0.5-1 ng/mL). There was a moderate positive significant correlationbetween serum phosphate and CRP (p=0.024 and r=0.404), there was a strong negative significant correlation between CRP and fetuinA (p=0.000 and r=-0.628). No significant correlation between the phosphate serum and fetuin A (p=0.365 and r=-0.168) was found.Based on this study, it was found that the higher serum phosphate level resulted in a higher CRP and lower fetuin A.]]>
<![CDATA[PHOSPHATIDYLINOSITOL -3KINASE (PI3K) DI PERBENIHAN ADIPOSIT YANG DIPAJAN GLUKOSA TINGGI DENGAN RETINOL ]]>
<![CDATA[Novi Khila Firani]]>;
<![CDATA[Bambang Prijadi]]>
<![CDATA[ INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 22, No 2 (2016) ]]>
Publisher : <![CDATA[Indonesian Association of Clinical Pathologist and Medical laboratory ]]>
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DOI: 10.24293/ijcpml.v22i2.1111
<![CDATA[Retinol is one of the active forms of vitamin A. In the previous study, it was known that retinol level in serum of DM patient waslower than in healthy people, which correlated with an increase of the glucose levels in these patients. The importance of retinol in insulinsignaling mechanisms that play a role in the pathogenesis of DM is still unknown. One of the components that play a role in insulinsignaling on adipocytes is phosphatidylinositol-3 kinase (PI3K), which encourages the translocation of glucose transporter-4 (GLUT4) tothe cell surface. The aim of this study was to know the importance of retinol therapy in the levels of PI3K enzyme on visceral adipocyteculture with high glucose exposure (25 mM) as a model of DM in vitro by determination method. Retinol therapy was given at a doseof 0.1 μM, 1 μM , and 10 μM. Measurement of PI3K level was done by ELISA method. The mean (SD) levels of PI3K enzyme were 1.91(0.27), 0.94 (0.15), 1.98 (0.22), 1.69 (0.81), 2.04 (0.16) ng/mL respectively, for adipocyte cultures exposed to 5mM glucose (as aphysiological condition), 25mM glucose, and 25mM glucose concentration with doses of retinol therapy 0.1 μM, 1 μM and10 μM. Theresults of this study indicated that high glucose exposure (25 mM) decreased the level of PI3K compared with adipocyte’s culture on5 mM glucose exposure. Retinol therapy with a dose of 0.1μM, 1μM and10 μM on adipocyte culture exposed with high glucose couldincrease the levels of PI3K.]]>
<![CDATA[RANCANGAN PRIMER SPESIFIK GEN MACROPHAGE MANNOSE RECEPTOR (MMR) UNTUK POLYMERASE CHAIN REACTION (PCR) DAN SEKUENSING DEOXYRIBO NUCLEIC ACID (DNA) ]]>
<![CDATA[Yani Triyani]]>;
<![CDATA[Nurizzatun Nafsi]]>;
<![CDATA[Lelly Yuniarti]]>;
<![CDATA[Nanan Sekarwana]]>;
<![CDATA[Endang Sutedja]]>;
<![CDATA[Dida Ahmad Gurnida]]>;
<![CDATA[Ida Parwati]]>;
<![CDATA[Bachti Alisjahbana]]>
<![CDATA[ INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 22, No 2 (2016) ]]>
Publisher : <![CDATA[Indonesian Association of Clinical Pathologist and Medical laboratory ]]>
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DOI: 10.24293/ijcpml.v22i2.1120
<![CDATA[The order (sequencing) determinationof DeoxyribonucleicAcid (DNA) bases is the gene’s most basic information, using the methodof Polymerase Chain Reaction (PCR) as its stage. A key factor of successful detection by PCR is specific PCR primer design choice. Thedetection of diversity of Mycobacterium Mannose Receptor (MMR) gene, responsible for recognizing mannosylated antigen structureof Mycobacterium tuberculosis (M.tb) by DNA sequencing of exon 7 chromosome 10p12, related to susceptiblity for PulmonaryTuberculosis(TB), was first performed in China in 2012. The purpose of this study was to find specific primerfromboth design originatedfrom the research in China/primer I and my own design/primer IIby using Primer3 software. This study was based on 10 healthy subjectsand was a preliminary study of a research titled. The Relationship of Single Nucleotide Polymorphisms (SNPs) of Macrophage MannoseReceptor Gene to Pulmonary Tuberculosis Cases. The examination materials consist of 3 mL of EDTA blood and DNA extraction from itsbuffy coat. The resulting DNA was processed by PCR to amplify MMR gene with primer I and II. The primer I successfully amplified DNAfragments up to 780bp while primer II only 329 bp. The MMR gene DNA sequencing analysis was performed on the amplification resultof both kinds primers by using DNA Baser and Ensembl−BLAST software. The results were different, DNA sequencing result by using theprimer I was found in several chromosomes and also in several loci. Whereas, by using the primer II, it was only found in chromosome10 and in the same locus. Based on this study, it can be concluded that the specific primer design is one of the most important factorsin the success of DNA sequencing.]]>
<![CDATA[PERMINTAAN DARAH PERSIAPAN TINDAKAN BEDAH DI RSUP DR. WAHIDIN SUDIROHUSODO ]]>
<![CDATA[Herlinah Herlinah]]>;
<![CDATA[Rachmawati Muhiddin]]>;
<![CDATA[Mansyur Arif]]>
<![CDATA[ INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 22, No 2 (2016) ]]>
Publisher : <![CDATA[Indonesian Association of Clinical Pathologist and Medical laboratory ]]>
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DOI: 10.24293/ijcpml.v22i2.1115
<![CDATA[Surgical procedures frequently need blood transfusion. However, the blood demand is frequently excessive without an appropriateneed analysis. The high percentage of canceled and returned blood indicates the ineffective use of blood transfusion. To know theappropriateness of blood demand for surgery preparation at the Dr. Wahidin Sudirohusodo General Hospital the researchers analyzedhis matter. This study was an observational study with cross sectional approach. The data of the blood demand for surgery preparationwere obtained from the blood bank of the Dr. Wahidin Sudirohusodo General Hospital, Makassar during August up to November 2013.The data were grouped based on the number of blood demand and the quantity of canceled and returned blood, these were then analyzedby SPSS software. The total of samples which were identified was about 1599 with 3829 blood demands. The quantity of canceled bloodwas1915 and the returned blood was 730. The demand of blood products indicated that whole blood was 2340 (61.3%), PRC 1392(36.4%), platelets 83 (2.2%) and FFP 5 (0.1%). The highest blood demand was observed in the General Surgery Department 797(20.9%). The difference between blood demand and the returned blood quantity (for whole blood and PRC) was analyzed statisticallyand indicated a significant result (p=0.000), this meant that there was a mismatch between blood demand number and the returnedblood quantity. This matter could reflect the ineffective use of blood transfusion.]]>
