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INDONESIA
Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML)
Published by Perhimpunan Dokter Spesialis Patologi Klinik Indonesia
ISSN : 08544263     EISSN : 24774685     DOI : https://dx.doi.org/10.24293
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Neuroscience
Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) is a journal published by “Association of Clinical Pathologist” professional association. This journal displays articles in the Clinical Pathology and Medical Laboratory scope. Clinical Pathology has a couple of subdivisions, namely: Clinical Chemistry, Hematology, Immunology and Serology, Microbiology and Infectious Disease, Hepatology, Cardiovascular, Endocrinology, Blood Transfusion, Nephrology, and Molecular Biology. Scientific articles of these topics, mainly emphasize on the laboratory examinations, pathophysiology, and pathogenesis in a disease.
Arjuna Subject : Kedokteran - Kedokteran (Lain-Lain)
Articles 1,328 Documents
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THE CORRELATION OF PROCALCITONIN AND MYELOPEROXIDASE INDEX LEVELS IN SEPSIS PATIENTS Sri Rejeki Wulandari; Betty Agustina Tambunan; Paulus Budiono Notopuro; Hardiono Hardiono
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 25, No 3 (2019)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v25i3.1451

Abstract

Sepsis masih menjadi masalah utama di dunia. Europan Society of Intensive Care Medicine (ESICM) dan Society of Critical Care Medicine (SCCM) mengikutsertakan quick Sequential Organ Failure Asssessment  (qSOFA) untuk mendiagnosis sepsis. Diperlukan pemeriksaan laboratorium akurat dan cepat selain kultur. Prokalsitonin sebagai penanda spesifik infeksi bakteri. Myeloperoxidase index (MPXI) parameter baru untuk membantu diagnosis sepsis. Penelitian ini bertujuan menganalisis korelasi kadar prokalsitonin dengan MPXI pada pasien sepsis.  Jenis penelitian cross sectional observasional. Pengambilan sampel Desember 2017  – Februari 2018. Subjek penelitian terdiri dari 71 pasien sepsis yang dirawat di Ruang Resusitasi, Ruang Observasi Intensif, dan ruang Intensive Care Unit (ICU) RSUD Dr. Soetomo Surabaya berdasarkan kriteria qSOFA dan SIRS. Pemeriksaan prokalsitonin dengan metode CLIA (ADVIA Centaur XP), MPXI dengan  metode  flowcytometry (ADVIA 2120i) dan kultur menggunakan alat PhoenixTM 100. Kadar prokalsitonin 0,01 ng/mL – 265,16 ng/mL (rerata 16,13 ± 40,91 ng/mL). Nilai MPXI -25,5 – 4,6 (rerata -7,939 ± 4,903). Tidak terdapat korelasi antara kadar prokalsitonin dengan MPXI ( p = 0,604 dan r = - 0,063). Tidak terdapat  korelasi kadar prokalsitonin dengan MPXI pada hasil  kultur positif (p = 0,675, r = 0,072) dan negatif (p = 0,401, r = - 0,147). Kadar prokalsitonin tidak berkolerasi dengan MPXI pada pasien sepsis
CRYPTOSPORIDIOSIS PARU DI HIV DAN AIDS JS. Hutagalung; R. Heru Prasetyo; Erwin Astha Triyono
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 19, No 1 (2012)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v19i1.386

Abstract

Although the prevalence of intestinal cryptosporidiosis in Indonesian HIV and AIDS patients were high, however the prevalence of pulmonary cryptosporidiosis have not been previously reported. The objective of this study was to know the determination of the pulmonary cryptosporidiosis prevalence in HIV and AIDS patients with pulmonary symptom that was treated in Dr. Soetomo General Hospital Surabaya. The detection of Cryptosporidium in sputum samples used modified versien acid fast stain of Ziehl Neelsen technique. In this study was found that three (3) of the eight (8) sputum samples (37.5%) of ≥55 years old and CD4≤70 HIV and AIDS patients were Cryptosporidium positive. Based on this study the HIV and AIDS patients with pulmonary symptoms should be suspect having the possibility of pulmonary cryptosporidiosis beside suffered tuberculosis.
MULTIDRUG-RESISTANT ACINETOBACTER BAUMANNII PREVALENCE AND CHARACTERISTICS IN DR.WAHIDIN SUDIROHUSODO GENERAL HOSPITAL OF MAKASSAR Dewi Tungadi; Nurhayana Sennang; Benny Rusli
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 25, No 2 (2019)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v25i2.1363

Abstract

BackgroundMultidrug-resistant Acinetobacter baumannii (MDRAB) is a strain of Acinetobacter baumannii which is resistant to three or more classes of antibiotics. As prevalence of MDRAB increases, the antibiotics of choice become limited. Identification of MDRAB is required to manage and control infection.MethodThis was a retrospective study, conducted in Dr. Wahidin Sudirohusodo General Hospital of Makassar, dated from January to December 2016. Bacterial identification and antimicrobial susceptibility testing (AST) were performed using VITEK 2. The patient data were obtained from electronic medical records.Results and DiscussionA total of 323 Acinetobacter baumannii isolates were obtained, consisted of 188 isolates in January-June 2016 and 36 of which was MDRAB (19.15%) with the average length-of-stay 33 days; and 135 isolates in July-December 2016 and 31 of which was MDRAB (22.96%) with the average length-of-stay 27 days. MDRAB was mostly discovered from patients using 3 or more medical devices and on single antibiotic therapy. MDRAB isolates were mostly obtained in sputum and pus specimens, and majority of patients had respiratory diseases. The result of AST showed 100% and 96% susceptibility to Polymyxin B; 71.43% and 54.84% susceptibility to Amikacin; 66.67% and 50% susceptibility to Trimethoprim/Sulfamethoxazole in January-June and July-December 2016, respectively.Conclusion and SuggestionsThe prevalence of MDRAB in our hospital in 2016 was high, suggesting the needs to improve hospital infection prevention and control. Polymyxin B, Amikacin, and Trimethoprim/Sulfamethoxazole are the antibiotics of choice to treat MDRAB.
PROTEIN REKOMBINAN 38 KDA MYCOBACTERIUM TUBERCULOSIS DALAM INTERLEUKIN-2 DAN INTERLEUKIN-4 SERTA LIMFOSIT T CD3+ (The Mycobacterium Tuberculosis 38 kDa Recombinant Protein in Interleukin-2 and Interleukin-4 as well as CD3+ T Lymphocytes) Maimun Z Arthamin; Nunuk S Muktiati; Triwahju Astuti; Tri Yudani M Raras; Didit T Setyo Budi; Francisca S. Tanoerahardjo
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 21, No 3 (2015)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v21i3.1275

Abstract

Tuberculosis remains a serious global health problem despite the widespread use of the vaccine against tuberculosis (TB). Up tonow, the only available TB vaccine, Mycobacterium bovis BCG has a very wide efficacy range from 0 until 80 percent protection sothe development of a new vaccine is needed. The new protein as a candidate vaccine should be assessed for their immunogenicity. Thepurpose of this study was to examine whether Mycobacterium tuberculosis 38 kDa recombinant protein could stimulate a cellularimmune response especially CD3+T lymphocytes to express IL-2 and IL-4 in PBMC cultures. An experimental laboratory research oncultured PBMC of 3 groups consisting of TB patients, contacts of TB positive and healthy subjects, each group consisted of 8 subjects. AllPBMC cultures were induced by Mycobacterium tuberculosis 38 kDa recombinant protein, Purified Protein Derivative (PPD) and withoutantigen as a control. Expression of IL-2 and IL-4 CD3+ T lymphocytes was measured with flowcytometry. In healthy volunteers and TBcontacts there was a significant difference in the expression of IL-2 and IL-4 CD3+ T lymphocytes compared with no any treatment. Thehighest IL-2 expression was in healthy subjects [8.13 (0.622)] while the highest expression of IL-4 was in TB patients [6.436 (4.586)].Mycobacterium tuberculosis 38 kDa recombinant protein could induce the expression of IL-2 and IL-4 of CD3+ T lymphocytes in healthysubjects, TB contacts and TB patients and there were a significance differences in the expression of all groups.
POLA DAN SENSITIVITAS KUMAN DI PENDERITA INFEKSI SALURAN KEMIH Samirah Samirah; Darwati Darwati; Windarwati Windarwati; Hardjoeno Hardjoeno
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 12, No 3 (2006)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v12i3.869

Abstract

The bacterial and sensitivity pattern towards antimicrobials on urinary tract infections (UTI) patients are very important to beknown by clinicians to get a successful treatment. The bacterial and sensitivity pattern towards antimicrobials will be changed in differentplace and time, so that those should be analyzed routinely. To evaluate the bacterial and antimicrobials resistance pattern on urinarytract infections patients. A retrospective study on 220 urinary samples in January until December 2004 at Clinical Microbiology subunit of Dr.Wahidin Sudirohusodo hospital . Of 99 samples of UTI, Prevalence of UTI in woman (54.5%) were higher than man (45.5%).Most of sample (28 samples) were found in 0 to 15 years group. The most bacteries in urine were Escherichia coli (39.4%) and Klebsiella(26.3%). Amikacin was sensitive to all bacteries, while amoxicilin and ampicilin were resistance. Prevalence of UTI in women werehigher than in men. Incidens of UTI was highest in children group. The most bacteries in urine samples were Escherichia coli. Amikacinwas sensitive to all bacteries, while Amoxicilin and Ampicilin were resistance.
ADIPONEKTIN HIGH MOLECULAR WEIGHT DAN KEKAKUAN VASKULAR DI PENYAKIT DIABETES MELITUS TIPE 2 TERKAIT GABUNGAN GLIMEPIRIDE METFORMIN DOSIS TETAP Sutjahjo, Ari
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 21, No 2 (2015)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v21i2.1089

Abstract

Diabetic patients have the tendency to develop micro vascular and macro vascular complications due to the hyperglycaemic state. Thereare various types of oral hypoglycaemic agents available in Indonesia, nevertheless, these agents fail to decrease mortality and morbiditydue to the endothelial dysfunction in diabetic patients. It is not yet known whether glimepiride metformin fixed dose combination isable to improve the serum High Molecular Weight (HMW) adiponectin and vascular stiffness which are important parameters of thecardiovascular risk factors. This study was to know the effect of glimepiride metformin fixed dose combination by investigation after 12weeks of treatment towards HMW adiponectin level and vascular stiffness using ba-PWV in type 2 diabetes. This research was carried outby a Quasi experimental study with pre and post test design. The subjects were type 2 diabetes patients who came to the EndocrinologyOutpatient Clinic at the Dr. Soetomo Hospital and 6 health centres in Surabaya during December 2010 to December 2011. In this study,measuring the HMW adiponectin level in plasma was by using ELISA method and the ba-PWV by using the V Serra-1000 before and aftertreatment with glimepiride metformin fixed dose for 12 weeks. Based on the thirty five type 2 diabetic patients who met the inclusion andexclusion criteria and who were enrolled in this study, after 12 weeks of treatment, the median HMW Adiponectin level was increasedfrom 1736 ng/mL to 1770 ng/mL, which was statistically not significant (p=0.317). After 12 weeks treatment, the median of ba-PWVwas decreased from 1580 cm/sec to 1450 cm/sec, which was statistically significant (p=0.028). The level of HMW adiponectin was notsignifantly different between before and after treatment. The level of brachial ankle Pulse Wave Velocety (ba-PWV) was significantlydifferent between before and after treatment. Glimepiride metformin fixed dose combination decreases the vascular stiffness, which isone of the parameters of the endothelial dysfunction.
DIAGNOSTIC VALUES OF MYCOBACTERIUM TUBERCULOSIS 38 KDA ANTIGEN IN URINE AND SERUM OF CHILDHOOD TUBERCULOSIS (Nilai Diagnostik Antigen 38 kDa Mycobacterium tuberculosis Air Kemih dan Serum di Tuberkulosis Anak) Agustin Iskandar; Leliawaty Leliawaty; Maimun Z. Arthamin; Ery Olivianto
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 23, No 1 (2016)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v23i1.1183

Abstract

Diagnosis TB anak sangat sukar karena gambaran klinis tidak khas, foto paru juga sulit diinterpretasi. Di anak sulit mendapatkandahak, jarang batuk dan jumlah kumannya sedikit. Deteksi antigen Mycobacterium tuberculosis merupakan sebuah pilihan yang tersediauntuk mendiagnosis TB. Teknik diagnosis TB secara serologis dan air kemih memberi banyak keuntungan karena mudah dikerjakan,biaya murah, cepat memberikan hasil dan mudah didapatkan, tidak menyakitkan serta tidak memerlukan spesimen dari jaringan yangsakit. Antigen 38 kDa merupakan antigen lipoprotein ekstraselular Mycobacterium sp memiliki potensi imunogen. Tujuan penelitian iniadalah membandingkan nilai diagnostik antigen 38 kDa Mycobacterium tuberculosis air kemih dan serum di tuberkulosis anak. Metodepenelitian merupakan kajian potong lintang dengan pengambilan sampel secara berurutan (Juni 2013-Juni 2014). Subjek penelitiansebanyak 54 anak yang terduga TB. Dilakukan pemeriksaan kadar antigen 38 kDa Mtb air kemih dan serum dengan metode ELISA.Hasil telitian ini didapatkan rerata kadar antigen 38 kDa air kemih dan serum subjek dengan TB lebih tinggi dibandingkan kelompokbukan TB, rerata/Simpang Baku (SD) antigen 38 kDa Mtb air kemih [0,25(0,388)] vs [0,03(0,011)] p=0,002, AUC (84,3%), Cut-offpoint: 0,04 ng/mL (kepekaan 83% dan kekhasan 71,43%) dan antigen 38 kDa Mtb serum [14,21(13,335)] vs [4,189(0,386)] p=0,263,AUC (63,5%), Cut-off point: 4,25 ng/mL (kepekaan 53,2% dan kekahasan 57,1%). Antigen 38 kDa Mtb air kemih lebih baik daripadaantigen 38 kDa Mtb serum untuk mendiagnostik tuberkulosis anak.
MYCOBACTERIUM TUBERCULOSIS DAN PCR Yuyun Widaningsih; Ismawati Amin; Nurhayana Sennang; Uleng Bahrun; Mansyur Arif
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 18, No 3 (2012)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v18i3.756

Abstract

TB’s coinfection in HIV and AIDS is on the increase together with the high mortality rate. The disseminated TB infection (in blood) of HIV and AIDS patients in Africa (2005) is about 10−25%. The aim of this study was to identify M.tb disseminated infection in HIV and AIDS patients. In this study was done in six HIV and AIDS patients suffering from suspected TB co infection. The sputum was tested using Fast Acid Bacilli and culture (using LJ and MGIT media) and DST. The identification of M.tb in blood used PCR with IS6110 primer (123 bp). The study was done at Dr. Wahidin Sudirohusodo Hospital, Labuang Baji Hospital, NEHCRI and at the Biomolecular Laboratory of the Medical Faculty, Hasanuddin University. From the six patients, four of them had CD4<50 sel/μL, two patients had FAB positive, three patients had positive LJ culture on day 41–47, three patients had positive MGIT culture on day 9–47 and four patients had positive PCR. In this study there was one case of negative FAB but LJ, MGIT and PCR were positive. There were two cases with positive FAB, culture and PCR, one case with negative FAB and culture but positive PCR. The M.tb disseminated infection was 66.7% in six HIV and AIDS patients.
SMALL DENSE LOW DENSITY LIPOPROTEIN WITH ANGIOGRAPHICALLY ATHEROSCLEROSIS IN CORONARY HEART DISEASE (Small Dense Low Density Lipoprotein dengan Aterosklerosis Secara Angiografi di Penyakit Jantung Koroner) Yuliani Zalukhu; Siti Muchayat Purnamaningsih; Nahar Taufik; Suwarso Suwarso
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 22, No 3 (2016)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v22i3.1243

Abstract

Penyakit Jantung Koroner (PJK) merupakan penyebab kematian utama di berbagai negara maju maupun yang berkembang danbiasanya terjadi bagi mereka yang memiliki kadar kolesterol tinggi, serta berusia lanjut. Namun baru-baru ini, penyakit jantung koronerlebih sering terjadi pada usia muda dan kadar kolesterol yang normal. Beberapa telitian menunjukkan bahwa LDL terdiri atas tujuhsubtype yaitu small dense Low Density Lipoprotein (sd-LDL) merupakan subtipe LDL yang jauh lebih aterogenik, sehingga sangat mudahmenyebabkan aterosklerosis. Tujuan penelitian ini adalah untuk mengetahui apakah terdapat hubungan antara sd-LDL dan persentaseaterosklerosis secara angiografi di penyakit jantung koroner. Rancangan penelitian adalah potong lintang, subjek penelitian 54 pasienPJK dipilih secara berurutan, yang menjalani pemeriksaan angiografi di RSUP. Dr. Sardjito, Yogyakarta. Penyakit jantung koronerditetapkan berdasarkan gejala klinis dan pemeriksaan EKG, aterosklerosis ditetapkan dengan pemeriksaan angiografi, sedangkansd-LDL merupakan angka banding LDL-C/Apo-B yang <1,2 diukur dengan metode enzimatik homogeneous dan Immunoturbidimetry.Kenasaban antara sd-LDL dengan aterosklerosis dianalisis dengan uji Spearman. Pada penelitian ini terteliti terdiri atas 37 laki-laki dan17 perempuan dengan gejala terbanyak nyeri dada 47(87%), diagnosis didominasi oleh angina pektoris stabil 49(90,8%) dan pengobatanpaling banyak adalah golongan statin. Ciri angka banding LDL-C/Apo-B serta persentase aterosklerosis subjek penelitian ditunjukkan diTabel 4, terlihat bahwa sd-LDL mempunyai rerata 1,06 dengan nilai minimal 0,81 dan maksimal 1,16, serta large buoyant LDL memilikirerata 1,34 dengan nilai minimum 1,20 dan nilai maksimum 1,48, sedangkan persentase aterosklerosis bernilai rerata 46,68% dengannilai minimal 0% dan maksimal 100%. Ditemukan kenasaban negatif, sedangkan yang bermakna antara small dense Low DensityLipoprotein (sd-LDL) dengan persentase aterosklerosis secara angiografi (r=-0,451; p=0,014).
IMUNOKIMIAWI PGE2 USUS ILEUM YANG KEKURANGAN ENERGI PROTEIN R. Heru Prasetyo
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 17, No 3 (2011)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v17i3.1057

Abstract

Diarrhoeal disease mortality which suffered by children less than five years due to protein energy deficiency is increased significantly.The pathological mechanisms are not known in detail yet. The objective of this research was able to explain the intestine motility inprotein energy deficiency of mice Balb/c. Hormonal mechanism that regulates the intestine motility is roled by prostaglandine E2 hormone(PGE2). The observed variable was the immunity component of PGE2. The measurement was performed using immunohistochemistrymethod with monoclonal antibody against PGE2 to the ileum mice tissue. The result of this study showed that protein energy deficiencymice had much reduced PGE2 (reduction from 1492 to 145, p = 0.000 ). In conclusion, the marked decrease of ileum PGE2 will decreaseintestine motility and it may be one cause of the high diarrhoeal mortality due to protein energy deficiency.

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