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Dr. dr. Puspa Wardhani, SpPK
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Kota adm. jakarta selatan,
Dki jakarta
INDONESIA
Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML)
ISSN : 08544263     EISSN : 24774685     DOI : https://dx.doi.org/10.24293
Core Subject : Health, Science,
Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) is a journal published by “Association of Clinical Pathologist” professional association. This journal displays articles in the Clinical Pathology and Medical Laboratory scope. Clinical Pathology has a couple of subdivisions, namely: Clinical Chemistry, Hematology, Immunology and Serology, Microbiology and Infectious Disease, Hepatology, Cardiovascular, Endocrinology, Blood Transfusion, Nephrology, and Molecular Biology. Scientific articles of these topics, mainly emphasize on the laboratory examinations, pathophysiology, and pathogenesis in a disease.
Articles 1,328 Documents
HYBRIDIZATION-BASED NUCLEIC ACID AMPLIFICATION TEST TERHADAP CARTRIDGE-BASED NUCLEIC ACID AMPLIFICATION TEST TERKAIT MULTIDRUG-RESISTANT TUBERCULOSIS (Hybridization-Based Nucleic Acid Amplification Test towards Catridge-Based Nucleic Acid Amplification Test in Multidrug-Resistant Tuberculosis) Ivana Agnes Sulianto; Ida Parwati; Nina Tristina; Agnes Rengga I
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 21, No 3 (2015)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v21i3.1274

Abstract

Indonesia has high burden of multidrug-resistant tuberculosis (MDR-TB). Cartridge-based nucleic acid amplification test (CB-NAAT),which is recommended as a diagnostic method of MDR-TB by World Health Organization, is faster in achieving the result. This methoddetermines MDR-TB only from the rifampisin resistance, by detecting mutations that occur on the 81 bp hot-spot region of the rpoBgene. The isoniazid resistance is not included in the determination of MDR-TB by this method. Hybridization-based NAAT (HB-NAAT)detects MDR-TB not only from the rifampisin resistance (codon 526 and 531 rpoB gene), but also from the isoniazid resistance (codon315 katG gene). The aim of this study was to know the validity of the HB-NAAT in detecting MDR-TB using sputum with CB-NAATas the gold standard in a diagnostic study. All of 51 sputums were collected during June 2013 from patients suspected pulmonaryMDR-TB at Dr. Hasan Sadikin General Hospital. The result of CB-NAAT were 16 MDR-TB, 12 TB non MDR, and 23 non TB. HB-NAATexamination results were 3 MDR-TB, 25 TB non MDR (3 RMR, 6 IMR, 16 susceptible) and 23 non TB. The sensitivity of HB-NAAT was18.75% and specificity 100%. Low sensitivity values may due to the high mutation variations in the samples. So it could not be detectedonly by codons 526 and 531 for rifampisin resistance. For the detection of isoniazid resistance, HB-NAAT have optimal primer at lowconcentrations and it also need more than katG genes to detect isoniazid resistance. Based on this study, it can be conclued, that HBNAAThas low sensitivity but high specificity in the detecting MDR-TB.
GLYCATED ALBUMIN AND HBA1C IN DIABETIC NEPHROPATHY (Albumin Glikat HbA1c dan Penyakit Nefropati Diabetik) Elvan Dwi Widyadi; Jusak Nugraha; Ferdy Royland Marpaung
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 22, No 3 (2016)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v22i3.1242

Abstract

Nefropati diabetik merupakan komplikasi kronis yang sering terjadi di pasien penyakit Diabetus Melitus (DM), perubahan statusglikemik harus diketahui lebih awal untuk mencegah komplikasi lebih lanjut. HbA1c sering digunakan sebagai pengendali glikemikjangka panjang (tiga/3 bulan). Albumin terglikasi merupakan tolok ukur baru yang dikembangkan untuk mengendalikan glikemikdalam waktu yang lebih singkat (2 minggu). Penelitian ini bertujuan untuk mengetahui kenasaban Albumin Glikat (GA) terhadapHbA1c di pasien nefropati diabetik. Dari 89 pasien DM yang diteliti, 34 didiagnosis sebagai diabetes nefropati. Kadar HbA1c diukurdengan metode Turbidimetric Inhibition Immunoassay (TINIA) dan GA diperiksa secara enzimatik. Analisis statistik dilakukan denganmenggunakan kenasaban Pearson, yang bermakna pada p<0,005. Cut off untuk GA: adalah 16% dan untuk HbA1c: 6,5%. RerataGA: adalah 20,73% dan 7,42% HbA1c. Pada penelitian ini, dengan uji Pearson diketahui kenasaban yang baik antara GA dan HbA1c(r=0,785, p-nilai<0,0001). Albumin Glikat memiliki kenasaban yang kuat terhadap HbA1c. Oleh karena itu, GA dapat digunakan untukmendeteksi indeks glikemik dalam jangka waktu singkat (2 minggu) di pasien pengidap nefropati diabetik.
PLATELET-LYMPHOCYTE RATIO (PLR) MARKERS IN ACUTE CORONARY SYNDROME (Platelet Lymphocyte Ratio (PLR) sebagai Petanda Sindrom Koroner Akut) Haerani Harun; Uleng Bahrun; Darmawaty ER
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 23, No 1 (2016)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v23i1.1176

Abstract

Sindrom Koroner Akut (SKA) merupakan puncak manifestasi klinis aterosklerosis di arteri koroner. Inflamasi terjadi dari tahapawal pembentukan ateroma sampai ruptur plak dan trombosis. Trombosis memainkan peran penting dalam perjalanan penyakit SKA.Trombositosis dan limfopenia berhubungan dengan derajat inflamasi sistemik dan Rasio Platelet Limfosit (PLR) menjadi petanda baruyang melibatkan kedua tolok ukur hematologi tersebut. Penelitian ini merupakan penelitian retrospektif di Rumah sakit Dr. WahidinSudirohusodo Makassar dengan mengambil data pasien SKA UAP, STEMI, NSTEMI dan menilai PLR dari pemeriksaan darah rutin saatmasuk Rumah sakit. Data PLR dibandingkan berdasarkan jenis SKA, kemudian dibandingkan dengan kontrol sehat. Hasil penelitiandidapatkan 223 data pasien SKA UAP, STEMI dan NSTEMI masing-masing 89, 68, 66 data dan kontrol normal 198 data. Hasil ujistatistik Kruskal wallis menunjukkan perbedaan bermakna PLR antara pasien UAP, NSTEMI dan STEMI (p=0,011). Hasil uji post hocmenunjukkan perbedaan bermakna antara UAP dan NSTEMI (p=0,023), UAP dan STEMI (p=0,006), tetapi tidak berbeda bermaknaantara NSTEMI dan STEMI (p=0,827). Nilai PLR pasien SKA lebih tinggi dibandingkan dengan pembanding (p=0,037). Berdasarkanhasil penelitian didapatkan nilai PLR meningkat di SKA dibandingkan dengan kontrol normal. Nilai PLR di STEMI dan NSTEMI lebihtinggi dibandingkan UAP, kemungkinan berhubungan dengan pembentukan trombus dan infark miokard.
COMPARISON OF PERIPHERAL BLOOD ACTIVATED NK CELL PERCENTAGE BEFORE AND AFTER INDUCTION PHASE CHEMOTHERAPY IN PEDIATRIC ACUTE LYMPHOBLASTIC LEUKEMIA Syntia TJ; Endang Retnowati; Yetti Hernaningsih; I Dewa Gede Ugrasena; Soeprapto Ma’at
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 23, No 3 (2017)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v23i3.1208

Abstract

Leukemia Limfoblastik Akut (LLA) adalah keganasan sel progenitor limfoid yang berasal dari sumsum tulang dan ditandai proliferasileukosit. Kejadian LLA masih tinggi, sehingga perlu diteliti peran sel NK dalam melawan leukemia. Tujuan penelitian adalah untukmengetahui perbedaan persentase sel NK teraktivasi sebelum dan sesudah pengobatan induksi dan hubungan persentase sel NK teraktivasisebelum pengobatan induksi dengan keluaran kemoterapi pasien LLA anak. Penelitian analitik observasional dengan rancang banguncohort prospektif. Subjek penelitian 27 pasien di Ruang Rawat Inap Hemato-Onkologi Anak RSUD Dr. Soetomo Surabaya, antara bulanMaret–Juli 2016. Metode memeriksa flowcytometry menggunakan alat BD FACS CaliburTM reagen Fast Immune CD56FITC/CD69PE/CD45 Per CP No.katalog.5055879. Analisis statistik dengan uji Wilcoxon Signed Rank dan regresi logistik. Terdapat perbedaan bermaknarerata persentase sel NK teraktivasi sebelum pengobatan induksi 0,57% (SB 0,53%) dan sesudahnya 2,01% (SB 1,86%) p=0,000.Menunjukkan peningkatan bermakna sel NK teraktivasi sesudah pengobatan induksi. Kenasaban sel NK teraktivasi sebelum pengobataninduksi dengan keluaran kemoterapi berkurangnya gejala penyakit (remisi) dan meninggal R=0.723 berarti kenasabannya kuat.Peningkatan persentase sel NK teraktivasi sesudah pengobatan induksi disebabkan kerja kemoterapi meningkatkan hasil MICA/B dankerja activating receptors sel NK (NKG2D) yang bersifat sitotoksik yang kuat. Persentase sel NK teraktivasi sebelum pengobatan induksiyang rendah disebabkan mekanisme menghilangnya tumor di LLA. Terdapat perbedaan bermakna persentase sel NK teraktivasi sebelumdan sesudah pengobatan induksi. Hasilnya dapat menjadi peramal keberhasilan pemberian kemoterapi LLA anak. Persentase sel NKteraktivasi sebelum kemoterapi tahap induksi yang tinggi berpengaruh kuat terhadap keluaran kemoterapi berkurangnya gejala penyakitdan sebaliknya bila rendah berpengaruh terhadap kemungkinan yang bersangkutan meninggal. Diperlukan hasil jangka panjang sampaiselesai dalam pengelolaan pemberian pengobatan terkait.
THE DIFFERENCE OF PLASMA D-DIMER LEVELS IN ACUTE MYOCARDIAL INFARCTION WITH AND WITHOUT ST ELEVATION Desi Kharina Tri Murni; Adi Koesoema Aman; Andre Pasha Ketaren
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 23, No 2 (2017)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v23i2.1140

Abstract

D-dimer terlibat di tahap awal patofisiologi proses Infark Miokard Akut (IMA). Kenaikan Kadar D-dimer di IMA mencerminkanadanya trombosis. Penelitian ini bertujuan untuk mengetahui perbedaan kadar D-dimer plasma di IMA dengan ST elevasi dan tanpaST elevasi. Penelitian ini berupa analitik observasional dilakukan secara potong lintang di Instalasi Gawat Darurat RSUP. AdamMalik Medan, masa waktu bulan April–September 2015. Sampel dikelompokkan menjadi 2 kelompok yaitu sampel dengan diagnosaNSTEMI berjumlah 18 sampel dan sampel dengan diagnosa STEMI berjumlah 18 orang. Semua sampel diperiksa Kadar D-dimer plasma.Penelitian ini menunjukkan ada perbedaan kadar D-dimer plasma di IMA dengan ST elevasi (STEMI) dan tanpa ST elevasi (NSTEMI)yaitu kadar D-dimer di kelompok NSTEMI adalah 440,39±209,33 dan kelompok STEMI adalah 654,89±229,88 (nilai p<0,05). Kadarrerata D-dimer di kelompok STEMI lebih tinggi daripada kadar D-dimer di kelompok NSTEMI.
UJI DIAGNOSTIK METODE IMUNOSITOKIMIA NS1 VIRUS DENGUE, UNTUK DIAGNOSIS INFEKSI Nafiandi Nafiandi; Ellyza Nasrul; Rismawati Yaswir
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 18, No 2 (2012)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v18i2.1011

Abstract

NS1 protein is a nonstructural protein of dengue virus which are secreted into the blood. NS1 protein could be detected in blood upto nine days after the onset of illness that almost simultaneously with the occurrence of viremia. The purpose of this study is to knowthe result of diagnostic test method of immunocytochemical NS1 by RT-PCR on dengue virus infected patients which is diagnosed at Dr.M. Djamil hospital. Padang. The method of the research uses cross sectional analytically study with consecutive sampling of patientswho diagnosed as infected with dengue virus and treated in the interne ward of Dr. M. Djamil General Hospital Padang from Januaryup to August 2011. Patient ‘s venous blood was taken about 5 mL and then centrifuged 3500 rpm and then the serum is separated andstored at-20° C until the sample is examined. The examination of NS1 serum is carried out by immunocytochemical method followedby the inspection of RNA viruses and their serotypes by RT-PCR and continued by agarose gel electrophoresis on 1.5 up to 2%. From thesixty samples obtained showed: 71.6% tested positive immunocytochemical NS1, 28.4% negative immunocytochemical NS1, 76.7%positive RT-PCR and 23.3% negative RT-PCR, 23.9% Den-1, 43.3% Den-2, 26.1% Den-3, and 6.5% Den-4. The immunocytochemicalNS1 diagnostic test obtained RT-PCR sensitivity of 85%, specificity 71%, positive predictive value 90%, and negative predictive value59%. The immunocytochemical NS1 has a quite high sensitivity, and low specificity, where as the positive predictive value is quite high,but the negative predictive value is lower than the RT-PCR for the diagnosis of dengue virus infection.
TROMBOSITOPENIA PADA PENGOBATAN DENGAN HEPARIN B. Mulyadi,*; J. Soemarsono
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 13, No 3 (2007)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v13i3.913

Abstract

Heparin induced thrombocytopenia (HIT), a well known side effect of heparin therapy, occurs in 1–5% of adults exposed to heparin.Unlike other drug induced thrombocytopenia, HIT does not usually cause bleeding, but instead cause thrombosis about 50% of HIT.The thrombosis in HIT can lead to limb gangrene or even death. The Importance to know the HIT is the wide use of heparin led tothe increasing recognition of untoward complications including HIT, relatively uncommon but severe side effect of heparin therapy,unpredictable, and difficulty in diagnosing and treating HIT. HIT is mediated by an antibody that recognizes an epitope on the plateletfactor (PF4)-heparin complex. The platelet factor (PF4)-heparin complex binds to FcgRII receptor on the platelet surface and crosslinksthereceptors.Thisinducesintenseplateletactivationandaggregationandsimultaneouslyactivatesbloodcoagulationpathways,thesechangesareprobablythebasisofthethrombosiseventsinHIT.HITwasclassifiedintotype1and2baseonthepathogenesisandtheseverityofHIT.RegularplateletcountmonitoringisbestsuitedforearlydiagnosisofHIT.Functional(serotoninrelease,plateletaggregationtest)andantigenassays(solidphaseenzymeimmunoassay,fluidphase,andparticlegelimmunoassay)areavailabletoconfirmHIT.HITwasmadebaseontheclinicalfindingandlaboratoryexamination.OnceHITisclinicallysuspected,heparinshouldbestoppedimmediatelyandtreatmentwithanalternativeanticoagulant,waitingforlaboratoryconfirmationmaybecatastrophic.Earlydiagnosisof HIT will decrease the morbidity and mortality.
GAMBARAN SEROLOGIS IgM – IgG CEPAT DAN HEMATOLOGI RUTIN PENDERITA DBD D. Irwadi; M. Arif; Hardjoeno Hardjoeno
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 13, No 2 (2007)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v13i2.860

Abstract

The prevalence of Dengue Hemorrhagic Fever (DHF) in South Sulawesi is still high. Proper and immediate management is importantto reduce the disease morbidity and mortality The aims of this study are to know the between correlate in stages of the disease and valuesof blood IgM-IgG, routine haematology analyses as will as infection status. A cross sectional study was done at Wahidin SudirohusodoHospital Makassar in January – June 2006. The diagnosis of DHF following WHO criteria (1999) and Immunocromatographic unit(ICT) for sera IgM-IGg have been conducted. From 83 collected samples, 37 male (44.6%) and female 46 (55.4%) ranged betweenage 6 months up to 47 year old. The decrease of trombocyte counts (mean 70.120/μL) and increase of (mean 10.09%) were observed.Serological unit result showed 10 (12%) primary infections and 68 (82%) secondary infections. Immunoglobulin G could be detectedin the second day of fever. The samples, 56 (67.5%) were stage I, 15 (18.1%) were stage II, and 12 (14.4%) were stage III or IV, thatmeans there were correlation between infection status and disease’s stages (p<0.01). It can be concluded they routine blood analysisis still useful for diagnosis of DHF, because it was confirmed by rapid serological unit specifically for IgM-IgG in early phase of DHF. Sothey an adequate treatment came out in order to prevent severe case of the disease.
MICROORGANISM PATTERN ON NASAL CAVITY OF END STAGE RENAL DISEASE PATIENTS WITH REGULAR HEMODIALYSIS AND STAFFS IN HEMODIALYSIS INSTALLATION ADAM MALIK GENERAL HOSPITAL MEDAN Imelda Damayanti; Ricke Loesnihari; Syafrizal Nasution
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 25, No 1 (2018)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v25i1.1509

Abstract

Colonization is the presence and growth of microorganism on the skin or mucous membrane of the body without any sign of infection. Commonly colonized microorganisms are normal flora within a person's body, but can also be microorganism in the hospital that colonizes within the body a few days after they enter the hospital. The longer a person is hospitalized the more likely to be colonized with the microorganism in the hospital. The objective is to determine microorganism pattern and antimicrobial sensitivity in the nasal cavity of regular hemodialysis patients and staff in Hemodialysis Installation of Adam Malik Hospital Medan. The study was analytic observational with a cross-sectional method, conducted in the Department of Clinical Pathology of Adam Malik Hospital Medan together with Hemodialysis Installation of Adam Malik Hospital Medan from August 2016 - October 2016. The sample was anterior nasal cavity swab which went through identification and sensitivity test. A total number of participants following the study were 46 people where patients and staffs were equal to 23 people. Most bacterial colonization of the nasal cavity of patients and staffs: Staphylococcus epidermidis 9 (39.13%) and 12 (52.17%); Staphylococcus aureus 4 (17.39%) and 3 (13.04%); Staphylococcus saprophyticus 2 (8.70%) and 2 (8.70%). There were 3 MRSA from a total of 23 bacteria (13.04%) in regular hemodialysis patients. MRSA colonization was found in regular hemodialysis patients with 50% sensitivity level of Vancomycin.
DEFISIENSI VITAMIN D TERHADAP PENYAKIT (Vitamin D Deficiency and Diseases) Pusparini Pusparini
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 21, No 1 (2014)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v21i1.1265

Abstract

It has been estimated that deficiency and insufficiency of Vitamin D affect one bilion people worldwide. Vitamin D deficiency can befound not only in countries with four (4) seasons, but also in countries with sunlight exposure all year long The objective of this studywas to know whether vitamin D deficiency can occur in Indonesia as well and to explore the role of vitamin D in people‘s health, althoughIndonesia is a country in the equator region. To avoid long term negative health consequences 25 hydroxyvitamin D/25 (OH) D serumlevel should be between 30 and 100 ng/mL. The main source of vitamin D is by synthesis at the skin which is exposed to ultraviolet Bradiation. The other source of vitamin D is from food. There are a lot of causes of vitamin D deficiency, for example: decreased vitaminD synthesis, nutritional intake of vitamin D, maternal vitamin D stores and exclusive breastfeeding, mal absorption and decreasedsynthesis or increased degradation of 25 (OH) D. From the above factors, decreased vitamin D synthesis is the main cause of vitamin Ddeficiency. The vitamin D deficiency is estimated and plays an important role in multiple disorders, such as: osteoporosis, fracture, cancer,cardiovascular disease, diabetes mellitus, autoimmune disease and infectious disease. A good strategy in managing vitamin D deficiencyis needed in order to solve the related problems.

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