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Fitrah al anshori
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Kampus 1 Universitas Cokroaminoto Palopo. Jl.Latamacelling No. 19
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INDONESIA
Jurnal Biogenerasi
Published by Universitas Cokroaminoto Palopo
ISSN : 25795163     EISSN : 25797085     DOI : -
Core Subject : Education,
Education Languange, Linguistic, Communication & Media Other
Biogenerasi focuses on the publication of the results of biology education,Biology learning model, Biology learning media, Biology Education Development, The article published on the internal and external academic community Universitas Cokroaminoto Palopo especially in Biology Education.
Arjuna Subject : Umum - Umum
Articles 939 Documents
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Evaluasi Ekstrak Bunga Asoka sebagai Indikator Alami Deteksi Boraks pada Sampel Makanan Olahan Desy Purwaty; Sartika Magdalena Sihite; Ilham Pratama; Santa Ceacilia Br Tarigan; Stevani Putri Christie Gulo; Eka Setiawan; Larasati Arum Utami
Jurnal Biogenerasi Vol. 11 No. 2 (2026): April - Juni 2026
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/fxv1ad60

Abstract

Borax is a prohibited food additive but is still found in processed foods in Indonesia. This study aims to evaluate Saraca asoca leaf extract as a natural indicator for borax detection. The extract was prepared using 70% ethanol and tested on borax solutions and food samples. The results showed a color change from brownish-green to yellow with increasing borax concentration, starting at 1.0%. Similar changes in several samples indicated the presence of borax. This change is related to interactions between flavonoid and tannin compounds and borate ions. Therefore, Saraca asoca extract has potential as a simple natural indicator for visual borax detection
IMPLEMENTASI PEMBELAJARAN IPA BERBASIS ONLINE LEARNING BERBANTUAN E-BOOK INTERAKTIF UNTUK MENINGKATKAN KETERAMPILAN BERPIKIR KREATIF Nur Rahmah Sangkala; Musawwir Usman; Akhmad Syakur
Jurnal Biogenerasi Vol. 11 No. 2 (2026): April - Juni 2026
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/bykr9k12

Abstract

Digital transformation in education encourages the integration of technology in science learning to support the development of 21st-century skills, one of which is creative thinking skills. This study aimed to determine the improvement of students’ creative thinking skills through the implementation of science learning assisted by an interactive e-book on the subtopic of Nutrition in Food. This study employed a pre-experimental research design using the One Group Pretest-Posttest Design. The research sample consisted of 23 eighth-grade students of SMP Piri 1 Yogyakarta selected using a saturated sampling technique. Data were collected using an essay-based creative thinking skills test covering the aspects of fluency, flexibility, originality, and elaboration. The data were analyzed using descriptive quantitative analysis with n-gain calculation. The results showed that the implementation of science learning assisted by an interactive e-book was able to improve students’ creative thinking skills. The n-gain results indicated that 3 students were categorized as high and 20 students were categorized as moderate. Improvements also occurred in each aspect of creative thinking skills, namely fluency by 30.5%, flexibility by 18.9%, originality by 26.1%, and elaboration by 22.3%. The use of interactive e-books in science learning was able to create a more engaging, interactive, and flexible learning experience, thereby supporting the development of students’ creative thinking skills.
PENGARUH AIR SUMUR DAN AIR SABUN TERHADAP AKTIVITAS MITOSIS SEL AKAR BAWANG (Allium cepa L. Christian Yopie Daniel Siregar Siagian; Siti Annisa Kusbama; Khory Aulia; Nazla Suhaila Parinduri; Santi Dameana Habeahan; Larasati Arum Utami; Hestia Hairima
Jurnal Biogenerasi Vol. 11 No. 2 (2026): April - Juni 2026
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/98kk3z97

Abstract

Mitosis is a cell division process that plays an important role in plant growth. This study aimed to determine the effect of well water and soapy water on the mitotic activity of onion root cells (Allium cepa L.). This research used a descriptive observational method through laboratory practicum with onion roots as the research object. Data were collected through direct observation using a light microscope on mitotic phases of onion root preparations stained with acetocarmine. The results showed that well water produced better root growth and mitotic activity than soapy water. The phases of prophase, metaphase, anaphase, and telophase were more clearly observed in the well water treatment, while fewer dividing cells and damaged tissues were found in the soapy water treatment. It can be concluded that soapy water has a negative effect on the mitotic activity of onion root cells.
ANALISIS FECAL COLIFORM PADA AIR LIMBAH MENGGUNAKAN METODE MOST PROBABLE NUMBER (MPN) Siti Nabila Nabila; Endang Sulistyarini Gultom
Jurnal Biogenerasi Vol. 11 No. 2 (2026): April - Juni 2026
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/xmcbmm96

Abstract

Domestic wastewater has the potential to pollute the environment because it contains pathogenic microorganisms, including fecal coliform bacteria as indicators of biological contamination. This study aimed to determine the presence of fecal coliform in wastewater samples using the Most Probable Number (MPN) method. The research was conducted from February 2 to February 6, 2026, at the Public Health Laboratory Center (BLKM) Medan. The study used a descriptive method with a five-tube MPN technique through presumptive testing using Lauryl Tryptose Broth (LTB) medium and confirmed testing using EC Broth referring to EPA Method 1680. The results showed variations in fecal coliform MPN values in each wastewater sample. Samples AL8, AL9, AL11, and AL12 showed the highest MPN value of >1600 MPN/100 mL, while samples AL1, AL2, and AL6 showed the lowest MPN value of <1.8 MPN/100 mL. The MPN method using LTB and EC Broth media was effective in detecting fecal coliform contamination in wastewater samples.
Analisis Cemaran Salmonella pada Makanan dengan Metode Compact Dry melalui Media Buffered Peptone Water Valdo Rekson Nainggolan; Endang Sulistyarini Gultom
Jurnal Biogenerasi Vol. 11 No. 2 (2026): April - Juni 2026
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/t6d4j218

Abstract

Keamanan pangan merupakan aspek krusial dalam menjaga kesehatan masyarakat dari penyakit bawaan makanan (foodborne disease) akibat kontaminasi bakteri patogen seperti Salmonella sp. pada berbagai tahapan pangan, khususnya produk hewani yang tidak diproses secara higienis. Infeksi bakteri Gram negatif ini dapat menyebabkan salmonellosis dengan gejala gangguan pencernaan hingga komplikasi serius pada kelompok rentan, sehingga pemeriksaan mikrobiologi pangan sangat penting untuk melindungi konsumen. Dalam analisisnya, tahap pra-pengkayaan (pre-enrichment) menggunakan media cair non-selektif Buffer Peptone Water (BPW) menjadi langkah awal yang sangat vital untuk memulihkan sel bakteri yang stres atau rusak akibat pengolahan, sekaligus meningkatkan sensitivitas deteksinya. Oleh karena itu, kajian ini bertujuan untuk membahas prosedur pemeriksaan Salmonella sp. pada sampel makanan serta teknik pembuatan media BPW. Melalui pemahaman metode ini, diharapkan pengetahuan mengenai pengujian mikrobiologi pangan dapat meningkat sebagai upaya preventif terhadap kontaminasi bakteri pada makanan.
Analisis Coliform Pada Sampel Air Limbah Menggunakan Metode Most Probable Number Deka Wira Pratama; Endang Sulistyarini Gultom
Jurnal Biogenerasi Vol. 11 No. 2 (2026): April - Juni 2026
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/emwhgg03

Abstract

Penelitian ini bertujuan untuk menganalisis keberadaan bakteri coliform pada sampel air limbah menggunakan metode Most Probable Number (MPN). Air limbah yang tidak diolah dengan baik berpotensi menjadi sumber pencemaran lingkungan dan penyebaran mikroorganisme patogen. Digunakann media Lauryl Tryptose Broth (LTB) pada uji pendugaan dan Brilliant Green Lactose Bile Broth (BGLB) pada uji penegasan. Sebanyak 15 sampel air limbah dianalisis untuk menentukan jumlah bakteri coliform. Hasil penelitian menunjukkan bahwa sebagian besar sampel terkontaminasi bakteri coliform dengan nilai MPN yang bervariasi antara <1,8 hingga >1600 MPN/100 mL. Beberapa sampel menunjukkan tingkat pencemaran yang sangat tinggi, sedangkan beberapa lainnya tidak terdeteksi mengandung coliform. Perbedaan tingkat pencemaran dipengaruhi oleh kondisi sanitasi, kandungan bahan organik, suhu, dan karakteristik sumber limbah. Hasil penelitian menunjukkan bahwa sebagian besar sampel belum memenuhi standar kualitas mikrobiologis sehingga diperlukan pengelolaan limbah yang lebih baik.
KARAKTERISASI ISOLAT BAKTERI RIZOSFER JAGUNG HIBRIDA (Zea mays L.) PADA TANAH MASAM Akhmad
Jurnal Biogenerasi Vol. 10 No. 4 (2025): Volume 10 nomor 4 tahun 2025 Terbit Oktober-Desember 2025
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/cq4z0s16

Abstract

Acidic soil is one of the major constraints in maize cultivation because low soil pH can increase the solubility of Al and Fe, inhibit root growth, and reduce nutrient availability, particularly phosphorus. Rhizosphere bacteria have the potential to support plant growth through various mechanisms; therefore, the exploration of local bacterial isolates from acidic soil environments is important. This study aimed to isolate and characterize rhizosphere bacteria associated with hybrid maize grown in acidic soil in Moncongloe Village, Maros Regency. Bacterial isolation was carried out using serial dilution and cultivation on Nutrient Agar (NA) medium, followed by colony purification and characterization based on colony morphology, Gram reaction using 3% KOH, and catalase activity using 3% H₂O₂. The results showed that 14 bacterial isolates were obtained from maize rhizosphere samples for further characterization. All isolates showed a Gram-positive reaction, while the catalase test revealed that 11 isolates (78.57%) were catalase-positive and 3 isolates (21.43%) were catalase-negative. Differences in morphological and basic biochemical characteristics indicate the diversity of rhizosphere bacterial isolates capable of growing under acidic soil conditions. These isolates have the potential to serve as local microbial candidates for biofertilizer development; however, further studies are required to evaluate their PGPR functional traits, biosafety, and molecular identification.
Keanekaragaman Isolat Bakteri Rizosfer Tanaman Jagung (Zea mays L.) pada Tanah Masam di Kabupaten Maros Akhmad
Jurnal Biogenerasi Vol. 10 No. 4 (2025): Volume 10 nomor 4 tahun 2025 Terbit Oktober-Desember 2025
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/w0nnv838

Abstract

This study aimed to the diversity of rhizosphere bacterial isolates associated with maize (Zea mays L.) cultivated in acidic soils of Maros Regency, South Sulawesi Province. Acidic soils characterized by low pH and limited nutrients, but the ability of microorganisms can adapt according to their habitat. However, microorganisms, particularly rhizosphere bacteria, exhibit remarkable adaptability to such conditions. The research employed serial dilution and culture on Nutrient Agar (NA) media to isolate bacteria from rhizosphere soil samples. A total of 14 bacterial isolates were toward will different morphological characteristics. All bacterial isolates found are Gram-positive bacteria. The findings reveal a high diversity of rhizosphere bacterial isolates, suggesting their potential role in enhancing plant growth as biofertilizers. Further research is necessary to evaluate the physiological and molecular potential of these bacterial isolates in nitrogen fixation, phosphate solubilization, and the production of plant growth hormones. Additionally, exploring the potential application of these bacteria as biocontrol agents or biofertilizers could contribute to improving maize productivity in acidic soils
Keanekaragaman Jenis Mangrove Di Kawasan Hutan Mangrove Kota Tarakan Suang Andi Palimbong; Raflen Aril Gerungan; Subekti Nurmawati; Elizabeth Novi Kusumaningrum; Mustaking
Jurnal Biogenerasi Vol. 11 No. 2 (2026): April - Juni 2026
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/b1qaq858

Abstract

Penelitian ini bertujuan untuk mengetahui keanekaragaman jenis mangrove yang tumbuh di Kawasan Konservasi Hutan Mangrove Kota Tarakan. Pengambilan data dalam penelitian ini menggunakan metode Purposive Sampling dengan cara menarik garis transek mengarah tegak lurus dari garis Pantai ke arah darat dan pertimbangan kerapatan vegetasi dengan pengampilan sampel dilakukan pada 3 stasiun.  Hasil penelitian tentang keanekaragaman jenis mangrove yang tumbuh di Kawasan Konservasi Kota Tarakan menunjukkan bahwa jenis mangrove yang ditemukan sebanyak 7 jenis dan yang paling dominan adalah jenis mangrove Rhizophora Appiculata. Berdasarkan hasil analisis data dapat disimpulkan bahwa indeks keanekaragaman Jenis Mangrove di Kawasan Konservasi Hutan Mangrove dan Bekantan Di Kota Tarakan sebesar 1<H’≤1.8276 yang berarti tergolong kedalam kriteria sedang.

Page 94 of 94 | Total Record : 939

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