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INDONESIA
Jurnal Biogenerasi
Published by Universitas Cokroaminoto Palopo
ISSN : 25795163     EISSN : 25797085     DOI : -
Core Subject : Education,
Education Languange, Linguistic, Communication & Media Other
Biogenerasi focuses on the publication of the results of biology education,Biology learning model, Biology learning media, Biology Education Development, The article published on the internal and external academic community Universitas Cokroaminoto Palopo especially in Biology Education.
Arjuna Subject : Umum - Umum
Articles 939 Documents
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INDUKSI TUNAS Musa paradisiaca L. MENGGUNAKAN KOMBINASI IBA DAN BAP SERTA AIR KELAPA Firdha, Nadiatul; Dur, Sajaratud; Nila Selvia, Irda
Jurnal Biogenerasi Vol. 11 No. 2 (2026): April - Juni 2026
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/qqtxmd98

Abstract

Pisang kepok (Musa paradisiaca L.) menjadi komoditas unggulan Indonesia yang memiliki nilai ekonomi dan gizi tinggi. Namun, perbanyakan bibit secara konvensional seringkali lambat dan rentan terhadap penyakit. Penelitian ini bertujuan untuk mengetahui pengaruh kombinasi zat pengatur tumbuh (ZPT) IBA (Indole Butyric Acid) dan BAP (Benzylaminopurine) serta penambahan air kelapa terhadap induksi tunas pisang kepok secara in vitro. Metode penelitian menggunakan Rancangan Acak Lengkap (RAL) non-faktorial dengan empat perlakuan: A1 (MS + IBA 2 mg/L + BAP 3 mg/L), A2 (MS + IBA 3 mg/L + BAP 2 mg/L), B1 (MS + IBA 2 mg/L + BAP 3 mg/L + Air Kelapa 15%), dan B2 (MS + IBA 3 mg/L + BAP 2 mg/L + Air Kelapa 15%). Hasil penelitian menunjukkan bahwa perlakuan memberikan pengaruh nyata terhadap jumlah tunas, namun berpengaruh tidak nyata terhadap waktu muncul tunas, tinggi tunas, diameter tunas, jumlah daun, dan bobot basah eksplan. Perlakuan B1 merupakan media yang paling optimal untuk menginduksi jumlah tunas terbanyak (1,67 tunas), tinggi tunas tertinggi (3,32 cm), diameter tunas terbesar (1,42 cm), dan jumlah daun terbanyak (2,11 daun). Secara keseluruhan, kombinasi sitokinin yang lebih tinggi (BAP 3 mg/L) dibandingkan auksin (IBA 2 mg/L) didukung oleh penambahan air kelapa 15% memberikan hasil pertumbuhan  lebih stabil dan optimal pada fase induksi tunas pisang kepok.
UJI KEPRAKTISAN LKPD MATERI SISTEM PENCERNAAN BERBASIS PROBLEM BASED LEARNING KELAS XI SMA Fakhriyyah, Putri
Jurnal Biogenerasi Vol. 11 No. 2 (2026): April - Juni 2026
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/tpr8zc47

Abstract

This study was motivated by the low level of student engagement in biology learning, particularly in the digestive system topic, and the limited availability of engaging and understandable teaching materials. This research aimed to determine the readability and practicality of Student Worksheets based on Problem Based Learning (PBL) for eleventh-grade senior high school students. The research employed a qualitative descriptive approach and was conducted at SMA Negeri 3 Padang during the first semester of the 2025/2026 academic year. Data were collected through student readability questionnaires and teacher practicality questionnaires using a Likert scale. The results showed that the readability level of the worksheets reached an average score of 84.03%, categorized as very good. Meanwhile, the practicality level based on teacher responses reached an average of 92.29%, categorized as highly practical. These findings indicate that PBL-based worksheets are easy to use, attractive, and capable of supporting student activity and understanding in learning the digestive system. Therefore, the developed worksheets are appropriate to be used as supporting teaching materials in senior high school biology learning.
ANALISIS FILOGENETIK GEN atpA PADA GENUS Oryza MENGGUNAKAN PERANGKAT LUNAK MEGA DENGAN PENDEKATAN NEIGHBOR-JOINING Rafila, Zacki; Violita; Achyar, Afifatul
Jurnal Biogenerasi Vol. 11 No. 2 (2026): April - Juni 2026
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/1ptac474

Abstract

This study aims to analyze the phylogenetic relationship of the atpA gene in the genus Oryza using Molecular Evolutionary Genetics Analysis (MEGA) software. The atpA gene, which plays a role in ATP synthesis in chloroplasts, was selected because of its relatively conserved nature and relevance in plant molecular evolution studies. The atpA gene sequences from 13 Oryza species were obtained from the NCBI GenBank database and analyzed using the Neighbor-Joining (NJ) method with the Kimura 2-Parameter model to reconstruct the phylogenetic tree. The reliability of the tree branches was tested through a bootstrap analysis of 10,000 repetitions. The results showed a pattern of kinship consistent with existing taxonomic classifications, in which Oryza sativa tended to form a single clade with Oryza rufipogon as its closest ancestor, while wild species such as Oryza punctata and Oryza officinalis formed a separate and more divergent clade. The relatively high bootstrap values at several branches indicate the reliability of the phylogenetic inferences generated. These findings reinforce the hypothesis that rice domestication involved wild species genetically close to cultivated species and suggest that the atpA gene has the potential to be used as an effective molecular marker in phylogenetic studies of the genus Oryza.
Pengaruh Lama Fermentasi dan Kombinasi Biofertilizer Kotoran Kambing dengan AB Mix terhadap Pertumbuhan Kangkung (Ipomoea reptans Poir.) dalam sistem Hidroponik H. Muhiddin, Nurhayani; Syakur, Akhmad; putri damayanti; Sahrani U
Jurnal Biogenerasi Vol. 11 No. 2 (2026): April - Juni 2026
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/fhw3zr91

Abstract

Water spinach (Ipomoea reptans Poir.) is a leafy vegetable that is highly nutritious and has the potential to be cultivated using a hydroponic system with environmentally friendly organic fertilizers. This study aims to determine the effect of fermentation duration and the combination of goat manure biofertilizer with AB Mix on the growth of water spinach (Ipomoea reptans Poir.) in a hydroponic system. The biofertilizer was fermented for 7, 14, and 21 days, then applied to various treatment combinations. The results showed that 7-day fermentation produced the best results in the P125AB75 treatment, with an average leaf width of 1.60 ± 0.56 cm and leaf length of 5.37 ± 1.05 cm. Fourteen-day fermentation produced optimal growth in the P20AB100 treatment with a leaf width of 2.13 ± 0.15 cm and a leaf length of 5.35 ± 0.59 cm. Meanwhile, 21 days of fermentation showed the most significant results in the P30AB100 treatment with a leaf width of 2.30 ± 0.10 cm and a leaf length of 6.45 ± 0.46 cm. Increasing the fermentation period was proven to improve nutrient availability, enrich active metabolites, and stabilize organic compounds, thereby supporting more optimal vegetative growth of water spinach compared to pure manure. Thus, liquid biofertilizer from 21-day fermentation, especially in the P30AB100 combination, is the best treatment for enhancing water spinach growth in hydroponic systems.
Analisis Keterampilan Metakognitif Siswa Kelas VIII SMP Makassar Raya dalam Menjawab Soal Berbasis HOTS Rahmat, Irwandi
Jurnal Biogenerasi Vol. 11 No. 2 (2026): April - Juni 2026
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/2ne0yv49

Abstract

This study aimed to analyze the metacognitive skills of eighth-grade junior high school students in Makassar Raya in answering Higher Order Thinking Skills (HOTS)-based questions. The study employed a quantitative approach with a descriptive-analytical design. The participants were 27 students selected through total sampling. Data were collected through a HOTS-based essay test consisting of 10 items. Each student response was assessed using the Corebima (2008) metacognitive skills rubric, with a score range of 0–7. The data were analyzed using descriptive statistics, including frequency, percentage, mean, median, mode, and standard deviation. The results showed that the students’ metacognitive skills were dominated by a score of 4, achieved by 12 students (44.44%). A score of 3 was obtained by 7 students (25.93%), a score of 5 by 3 students (11.11%), scores of 2 and 6 by 2 students each (7.41%), and a score of 7 by 1 student (3.70%). No students obtained a score of 1. These findings indicate that the students’ metacognitive skills were in the moderate or transitional category. In general, the students were able to provide correct, coherent, logical, and well-reasoned answers, but they had not yet optimally expressed their responses in their own words. Therefore, HOTS-based instruction needs to be accompanied by strategies that encourage students to plan, monitor, and evaluate their thinking processes more reflectively.
Literature Review: Bakteri Asam Laktat Dari Dadih Minangkabau: Keragaman, Karakteristik, Dan Potensi Aktivitas Antibakteri Adzra Haura, Rahmi
Jurnal Biogenerasi Vol. 11 No. 2 (2026): April - Juni 2026
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/wmgnc484

Abstract

Dadih merupakan produk susu fermentasi tradisional khas Minangkabau, Sumatera Barat, yang diproduksi melalui fermentasi spontan susu kerbau dalam tabung bambu. Produk ini telah lama dikenal sebagai sumber bakteri asam laktat (BAL) yang kaya dan beragam, dengan potensi manfaat sebagai agen probiotik, antibakteri, antioksidan, dan biopreservatif alami. Artikel review ini mengintegrasikan temuan dari berbagai studi ilmiah mengenai keragaman spesies BAL yang diisolasi dari dadih, karakteristik morfologi dan biokimianya, serta potensi aktivitas antibakterinya terhadap bakteri patogen pangan, terutama Escherichia coli dan Staphylococcus aureus. Kajian literatur menunjukkan dominasi genus Lactobacillus, Lactococcus, Pediococcus, Enterococcus, Weissella, dan Leuconostoc dalam komunitas BAL dadih. Aktivitas antibakteri BAL dadih diperantarai oleh produksi senyawa bioaktif seperti asam laktat, hidrogen peroksida, diasetil, dan bakteriosin, dengan mekanisme kerja yang melibatkan permeabilisasi membran sel dan gangguan sintesis dinding sel patogen. Isolat BAL dari dadih secara konsisten menunjukkan penghambatan lebih kuat terhadap bakteri Gram-positif (S. aureus) dibandingkan Gram-negatif (E. coli), yang dikaitkan dengan perbedaan struktur dinding sel. Dadih Minangkabau berpotensi besar sebagai sumber BAL unggulan untuk pengembangan probiotik dan biopreservatif pangan berbasis kearifan lokal
Status Permudaan Alami Hutan Mangrove di Teluk Buo Bungus Kota Padang Safitri, Nadila; Leilani Eka Putri, Irma; Vauzia; Yulina Ade, Filza
Jurnal Biogenerasi Vol. 11 No. 2 (2026): April - Juni 2026
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/8kzxcf45

Abstract

Mangrove forests are important coastal ecosystems that function as shoreline protectors, carbon sinks, and habitats for many aquatic organisms. In Teluk Buo, Bungus, Padang City, mangrove stands have experienced pressure from land conversion and human activities, so information on natural regeneration is needed to support management efforts. This study aimed to determine the natural regeneration status of mangroves in Teluk Buo based on species density values. The study used a descriptive quantitative method with a plot sampling approach at three observation stations, each consisting of three plots selected by purposive sampling. Observation plots of 10 x 10 m were used for trees, 5 x 5 m for saplings, and 2 x 2 m for seedlings. Data were analyzed using species density and regeneration status criteria according to Shankar (2001). The results showed that two mangrove species were found, namely Rhizophora apiculata and Bruguiera gymnorrhiza. Station 1 had a seedling density of 75, sapling density of 12, and tree density of 26.33, indicating a sufficient regeneration status. Station 2 had a seedling density of 144, sapling density of 24, and tree density of 5.67, while Station 3 had a seedling density of 122, sapling density of 17.33, and tree density of 8.33; both stations indicated good regeneration status. Rhizophora apiculata was the dominant species at all growth levels, whereas Bruguiera gymnorrhiza showed limited regeneration. In general, natural regeneration of mangroves in Teluk Buo was classified as good to sufficient, with regeneration being more strongly supported by Rhizophora apiculata.
INVENTARISASI TANAMAN HIAS DI KAMPUS UNIVERSITAS NEGERI PADANG Almiah, Mina; Chatri, Moralita; Advinda, Linda; Yulina Ade, Filza
Jurnal Biogenerasi Vol. 11 No. 2 (2026): April - Juni 2026
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/yg3bpj84

Abstract

Inventarisasi tanaman hias merupakan langkah penting dalam mendukung pengelolaan ruang terbuka hijau dan penerapan konsep green campus di lingkungan perguruan tinggi. Penelitian ini bertujuan untuk mengidentifikasi jenis-jenis tanaman hias yang terdapat di Kampus Universitas Negeri Padang (UNP), mengetahui sebarannya, serta menganalisis fungsi ekologisnya. Penelitian dilaksanakan dengan menggunakan metode survei eksploratif melalui metode jelajah (cruise method). Data dikumpulkan melalui pengamatan langsung, dokumentasi, dan identifikasi tanaman berdasarkan karakter morfologi menggunakan literatur botani dan aplikasi identifikasi tanaman. Hasil penelitian menunjukkan bahwa di Kampus UNP ditemukan 12 spesies tanaman hias yang tergolong ke dalam 8 famili, dengan famili Araceae dan Asparagaceae sebagai famili yang paling dominan. Tanaman hias tersebar di berbagai area kampus seperti taman fakultas, halaman rektorat, jalur pedestrian, dan area parkir. Keberadaan tanaman hias tersebut tidak hanya berfungsi sebagai elemen estetika, tetapi juga memiliki peran ekologis penting seperti penyerap polutan udara, penghasil oksigen, pengatur suhu mikro, serta penunjang keanekaragaman hayati. Hasil inventarisasi ini diharapkan dapat menjadi data dasar dalam perencanaan dan pengelolaan lanskap hijau kampus yang berkelanjutan serta mendukung kegiatan pembelajaran dan konservasi lingkungan di Universitas Negeri Padang.
DISTRIBUSI DAN KARAKTERISTIK SAMPAH WISATA DI ZONA INTERTIDAL LABUAN BAJO SERTA IMPLIKASI AWAL TERHADAP EKOSISTEM PESISIR Taufik Kaisupy, Muhammad; Prasetyo Adi Wibowo, Singgih; Reza Cordova, Muhammad
Jurnal Biogenerasi Vol. 11 No. 2 (2026): April - Juni 2026
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/kw6jra20

Abstract

Labuan Bajo has experienced rapid tourism growth over the past decade, accompanied by increasing pressure on its coastal environments. This study aimed to examine the spatial distribution and composition of marine debris in intertidal areas and to provide an initial assessment of potential ecological implications. A descriptive survey approach was applied through field observations at selected coastal sites representing varying levels of tourism intensity. Marine debris was quantified using standardized sampling units and classified into major material categories, with further sub-classification for plastic items. The results showed that debris distribution varied across locations, with higher densities recorded in areas with intensive tourism activity. Plastic dominated the debris composition, particularly single-use packaging, plastic bags, and food-related items. Fragmented materials were also observed, indicating ongoing degradation processes that may contribute to microplastic formation. Differences in debris accumulation appeared to be influenced by local human activity and site characteristics, including accessibility and shoreline features. These findings suggest that tourism-derived debris has begun to exert measurable pressure on coastal ecosystems, especially in intertidal zones where biological interactions are frequent. While this study does not directly assess biological impacts, the observed patterns indicate potential risks related to ingestion, entanglement, and habitat disturbance. The study provides baseline information that can support future ecological monitoring and more detailed investigations in tourism-dominated coastal systems.
LITERATUR REVIEW: APLIKASI MOLEKULER BERBASIS PCR DALAM DETEKSI  DAN KARAKTERISASI Escherichia coli PADA SEKTOR LINGKUNGAN, PANGAN, DAN KLINIS Yunavita, Diva; Hilda Putri, Dwi
Jurnal Biogenerasi Vol. 11 No. 2 (2026): April - Juni 2026
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/jcp1q263

Abstract

Escherichia coli is a gram-negative bacterium and a key indicator of water and food contamination. Conventional PCR is a widely used molecular method for specific, rapid, and sensitive E. coli detection. This literature review systematically examines the application of conventional PCR in E. coli detection across water, food, and clinical sample matrices from 2017 to 2025. Thirteen scientific articles were reviewed regarding research objects, PCR methods, research stages, and findings. Conventional PCR using primers 16E1/16E2 (584 bp), Afa FP/RP (480 bp), and ESS (825 bp) effectively detected E. coli in diverse samples. PCR showed superior sensitivity (100%) and specificity (94.34%) compared to culture methods (Hariri, 2022). Direct PCR offered a cost-effective alternative without DNA extraction (JKG, 2025). Multiplex PCR enabled simultaneous detection of multiple virulence genes (Ekawati et al.; Microbiology Spectrum, 2024). Conventional PCR remains a reliable, specific, and rapid tool for E. coli detection across diverse sample types.

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