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Jurnal Biogenerasi
Published by Universitas Cokroaminoto Palopo
ISSN : 25795163     EISSN : 25797085     DOI : -
Core Subject : Education,
Education Languange, Linguistic, Communication & Media Other
Biogenerasi focuses on the publication of the results of biology education,Biology learning model, Biology learning media, Biology Education Development, The article published on the internal and external academic community Universitas Cokroaminoto Palopo especially in Biology Education.
Arjuna Subject : Umum - Umum
Articles 911 Documents
 88   89   90   91   92 
Penerapan Prinsip Bioetik dalam Pengujian Escherichia coli pada Air dan Salmonella pada Ikan Nila (Oreochromis niloticus) Menggunakan Media Compact Dry Berdasarkan Standar ISO/IEC 17025 di Laboratorium BPPMHKP Medan I Andina Ayu Syahputri; Dara Arifia, Dini; Situmorang, Nurbaity; Yusuf Nasution, Mhd; Arwita, Widya
Jurnal Biogenerasi Vol. 11 No. 2 (2026): April - Juni 2026
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/xk28gj62

Abstract

Food safety and environmental quality are critical aspects in maintaining public health, particularly in fishery products and water sources that are vulnerable to contamination by pathogenic microorganisms. This study aims to identify the presence of Escherichia coli in water samples and Salmonella in tilapia (Oreochromis niloticus), as well as to analyze the use of Compact Dry media based on ISO/IEC 17025 and the implementation of bioethical principles in microbiological testing. This research employed a descriptive laboratory approach conducted at the BPPMHKP Medan I Laboratory using Compact Dry media based on AOAC methods. The samples consisted of lake water and tilapia, which were tested through enrichment, inoculation, incubation, and colony observation stages. The results showed no growth of Escherichia coli colonies on Compact Dry EC media and no Salmonella colonies on Compact Dry SL media, indicating that all samples were negative for the tested bacteria. The use of Compact Dry media in this study complied with ISO/IEC 17025 standards, as indicated by systematic procedures, the use of validated media, and laboratory quality control. Furthermore, bioethical principles, including scientific integrity, responsibility, biosafety, and accountability, were applied throughout all stages of testing. Therefore, this study demonstrates that the use of validated media and the application of bioethical principles play an important role in ensuring valid, reliable, and accountable laboratory results.
Optimasi Primer : Suhu Annealing untuk Deteksi Gen Matrix Virus Influenza A Menggunakan  Polymerase Chain Reaction (PCR) Turnip, Natalia; Dilla Yanthi, Nova; Hilda Putri, Dwi
Jurnal Biogenerasi Vol. 11 No. 2 (2026): April - Juni 2026
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/e9cf4f40

Abstract

Influenza A virus (IAV) is a pathogen that attacks the respiratory tract in humans and animals, which can be a threat to public health. This virus has a number of genes that are targeted by PCR for diagnostic detection purposes, one of which is the matrix gene. Detection of the matrix gene in the Influenza A virus from DNA/RNA genetic material can be done using the polymerase chain reaction (PCR) technique. One of the stages that determines the success of the PCR process is the annealing stage, for which the optimum temperature and cycle are required for primer attachment in the annealing stage. This research is a descriptive study towards an experiment conducted in June 2025 at the Bacteriology Laboratory of the Research and Innovation Agency (BRIN) KST. Soekarno Cibinong. The results obtained showed that the optimum temperature for matrix gene amplification in the Influenza A virus using the forward primer FluAV M-U44 and reverse primer FluAV M-L287 was 53.5 °C, 54 °C, and 54,5 °C and 35 cycles were evidenced by the formation of a fairly bright band in the electrophoresis results.
Penerapan Prinsip Bioetika Dalam Analisis Total Coliform Pada Pengujian Air Limbah di Laboratorium Mikrobiologi Balai Stadarisasi dan Pelayanan Jasa Industri (BSPJI) Medan Husnah Humairoh, Aaqillah; Novauli Sidabutar, Esra; Putri Tampubolon, Nadia; Yusuf Nasution, Muhammad; Arwita, Widya
Jurnal Biogenerasi Vol. 11 No. 2 (2026): April - Juni 2026
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/bnsw2a64

Abstract

Penelitian ini bertujuan untuk menganalisis penerapan prinsip bioetika dalam pemeriksaan total coliform pada pengujian air limbah di Laboratorium Mikrobiologi Balai Standardisasi dan Pelayanan Jasa Industri (BSPJI) Medan. Pengujian kualitas air limbah penting dilakukan untuk mengetahui tingkat kontaminasi mikroorganisme yang berpotensi membahayakan kesehatan masyarakat dan lingkungan. Penelitian ini dilaksanakan pada bulan Februari–Maret 2026 dengan menggunakan metode Most Probable Number (MPN) yang terdiri dari dua tahap pengujian, yaitu uji praduga dan uji penegasan. Sampel yang dianalisis adalah air limbah dengan kode 0074 (inlet) dan 0075 (outlet). Hasil penelitian menunjukkan bahwa nilai total coliform pada sampel 0074 sebesar >1600 MPN/100 mL, sedangkan pada sampel 0075 sebesar 110 MPN/100 mL. Nilai tersebut masih berada di bawah baku mutu yang ditetapkan yaitu ≤3000 MPN/100 mL, sehingga menunjukkan bahwa proses pengolahan air limbah mampu menurunkan jumlah bakteri coliform. Selain itu, hasil analisis menunjukkan bahwa prinsip bioetika yang meliputi prinsip manfaat, kejujuran, keadilan, dan tidak merugikan telah diterapkan selama proses pengambilan hingga pemeriksaan sampel. Dengan demikian, penerapan prinsip bioetika dalam kegiatan pengujian laboratorium sangat penting untuk menjamin keselamatan kerja, integritas ilmiah, serta perlindungan terhadap lingkungan dan masyarakat.  
ANALISIS VEGETASI DI KAWASAN HUTAN NAGARI SIMPANG KAPUAK KECAMATAN MUNGKA KABUPATEN LIMA PULUH KOTA SUMATERA BARAT R, Atikah; Leilani Eka Putri, Irma; Kardiman, Reki; Yulina Ade, Filza
Jurnal Biogenerasi Vol. 11 No. 2 (2026): April - Juni 2026
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/pbcq3z65

Abstract

The Simpang Kapuak Village Forest, located in Mungka Subdistrict, Lima Puluh Kota Regency, is a forest area that plays a role in maintaining environmental balance and supporting the livelihoods of the surrounding communities. This area possesses a high level of biodiversity, making the structure of its forest community a valuable starting point for long-term management and conservation. Therefore, it is important to understand the structure and composition of the vegetation in the Simpang Kapuak Village Forest, Mungka Subdistrict, Lima Puluh Kota Regency. This study is a quantitative descriptive study. The was conducted in the Simpang Kapuak Village Forest, Mungka Subdistrict, Lima Puluh Kota Regency. Data collection was carried out using the single-plot method across 5 plots. Each plot consists of 4 subplots corresponding to the stand size, namely 2 x 2 m (seedlings), 5 x 5 m (saplings), 10 x 10 m (mature trees), and 20 x 20 m (large trees). The research data were analyzed to determine the Importance Value Index (IVI) of the species. The results of the study indicate that there are 27 species belonging to 16 families. The highest Importance Value Index (IVI) was recorded for Syzygium chloranthum at the tree level (134.25%), followed by Litsea grandis at the pole level (144.92%), Syzygium chloranthum at the sapling level (82.97%), and Synedrella nodiflora at the seedling level (32.02%).
Pengaruh Ekstrak Daun Alang Alang (Imperata cylindrica) terhadap Pertumbuhan Tanaman Cabai Merah (Capsicum annum L.) Putri Alizah, Alya; Anhar, Azwir
Jurnal Biogenerasi Vol. 11 No. 2 (2026): April - Juni 2026
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/qbjndk86

Abstract

Red chili peppers (Capsicum annum L.) are an important horticultural commodity with high economic value and play a significant role in meeting people's food needs. However, chili plant productivity often faces constraints due to biotic factors, one of which is interactions with weeds. Cogongrass (Imperata cylindrica) is known as an invasive weed that is generally detrimental, but it contains secondary metabolite compounds that have the potential to be utilized in agriculture. Allelopathic compounds produced by cogongrass can affect the growth of other plants, either inhibitively or stimulatively depending on the concentration and application method. This article aims to examine the potential of cogongrass leaf extract on chili plant growth based on the results of the latest scientific research. The writing method was carried out through a literature review of national and international journals published in 2020 and later. The results of the study indicate that plant extracts, including cogongrass, have the potential to be developed as environmentally friendly natural plant growth regulators, although precise dosage adjustments are required to avoid growth inhibitory effects.
POTENSI Bacillus licheniformis strain Nam 2-2 DALAM PRODUKSI BIOLISTRIK PADA VARIASI pH Silvi, Silvi Tamara; Irdawati; Advinda, Linda; Yuniarti, Elsa
Jurnal Biogenerasi Vol. 11 No. 2 (2026): April - Juni 2026
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/55fxme08

Abstract

The increasing global energy demand has encouraged the development of sustainable alternative energy sources such as Microbial Fuel Cells (MFCs). This study aimed to determine the optimum pH for bioelectricity production using Bacillus licheniformis strain Nam 2-2 in a dual-chamber MFC system. The experiment employed a completely randomized design with pH variations of 7, 7.5, and 8. Voltage output was measured every 2 hours over a 24-hour incubation period. The results showed that bioelectricity production increased over time, reached a peak at 16–20 hours, and then declined. The highest voltage was observed at pH 7.5, reaching 0.782 mV, followed by pH 8 (0.758 mV) and pH 7 (0.748 mV). These findings indicate that slightly alkaline conditions are more favorable for microbial activity and electron transfer efficiency. Therefore, pH optimization is essential to improve MFC performance and supports its potential as a renewable energy source.
PENGARUH ISOMETRIC HANDGRIP EXERCISE TERHADAP PERUBAHAN TEKANAN DARAH PADA LANSIA PENDERITA HIPERTENSI Hariyadi; Ayu Ananda Prianita, Elen; Hastuti, Rela; Suhartiningsih, Sri
Jurnal Biogenerasi Vol. 11 No. 2 (2026): April - Juni 2026
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/33v6tn26

Abstract

Hypertension or high blood pressure is one of the main health problems in the elderly that risks reducing the quality of life and increasing morbidity and mortality. Hypertension management efforts can be done pharmacologically and non-pharmacologically, one of which is by doing isometric handgrip exercise. This study aims to determine the effect of isometric handgrip exercise and its frequency on changes in blood pressure in elderly people with hypertension in X Village, Madiun Regency. This type of research uses a pre-experimental design with a one-group pretest-posttest design approach. The population of this study was elderly people with hypertension in X Village, Madiun Regency. The sample amounted to 18 respondents selected using a simple random sampling technique. Data were collected by measuring blood pressure before and after being given isometric handgrip exercise intervention for 5 consecutive days, then analyzed using the Wilcoxon signed rank test. The results showed that the average systolic blood pressure before the intervention was 151.67 mmHg and after the intervention became 133.33 mmHg, while the average diastolic blood pressure before the intervention was 91.67 mmHg and after the intervention became 77.78 mmHg. The results of the Wilcoxon test obtained a ρ-value = 0.000 (p < 0.05), which means there is a significant effect of isometric handgrip exercise on changes in blood pressure in elderly people with hypertension. This study shows that isometric handgrip exercise is effective in lowering blood pressure in elderly people with hypertension. This exercise can be used as a simple, safe, inexpensive, and easy to do non-pharmacological intervention independently at home.
ANALISIS STRUKTUR DAN KOMPOSISI VEGETASI PADA HUTAN KELOLA MASYARAKATAN (HKM) HARAPAN JAYA BATU AMPAR, NAGARI PANTI, KECAMATAN PANTI, KABUPATEN PASAMAN TIMUR, SUMATRA BARAT Dwi Ananda, Tiara; Leilani Eka Putri, Irma; Kardiman, Reki; Yulina Ade, Filza; Anhar, Azwir; Thanoesya, Ryan; Riangga Hanafi, Bayu
Jurnal Biogenerasi Vol. 11 No. 2 (2026): April - Juni 2026
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/wnjzjz31

Abstract

Vegetation structure refers to the stratification of plant growth stages within an ecosystem, which are generally classified into seedling, sapling, pole, and tree levels. Analysis of plant composition aims to identify plant species present in a forest area. This study aimed to determine the vegetation structure and plant composition in the Community Forest Management (HKM) area of Harapan Jaya Batu Ampar, Nagari Panti, Panti District, East Pasaman Regency, West Sumatra. The research employed a nested quadrat method with plots placed randomly, consisting of 10 plots adjusted to the field conditions at the study site. The results showed that the 2 × 2 m and 5 × 5 m plots were dominated by the family Rubiaceae, with Coffea robusta as the most abundant species. The 10 × 10 m plots were dominated by the family Lauraceae, with Litsea sp. as the dominant species. Meanwhile, the 20 × 20 m plots were dominated by the family Fagaceae, with Lithocarpus elegans recorded as the main species. These findings provide preliminary data for understanding the vegetation structure in the Community Forest Management (HKM) area of Harapan Jaya Batu Ampar, Nagari Panti, Panti District, East Pasaman Regency, West Sumatra
Analisis Pohon Filogenetik Genus Oryza Berdasarkan Gen matK Marizky Siregar, Yoana; Violita, Violita; Achyar, Afifatul
Jurnal Biogenerasi Vol. 11 No. 2 (2026): April - Juni 2026
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/6myncp72

Abstract

The genus Oryza includes both cultivated and wild rice species that play an important role in global food systems and crop improvement. These species also exhibit complex evolutionary patterns, making the study of their phylogenetic relationships essential for understanding domestication and genetic diversity. In this study, phylogenetic relationships within the genus Oryza were analyzed using chloroplast matK gene sequences through a bioinformatics approach. A total of twelve species, representing both cultivated and wild taxa, were selected from the GenBank database. Sequence alignment and tree reconstruction were carried out using MEGA version 11. The phylogenetic tree was generated using the Neighbor-Joining method with the Maximum Composite Likelihood model, and its reliability was assessed through 10,000 bootstrap replications. The results show a clear distinction between cultivated and wild Oryza species. Oryza sativa (Japonica and Indica) is closely associated with O. nivara, supported by high bootstrap values, indicating a strong evolutionary link. Meanwhile, several wild species form separate clades, suggesting earlier divergence within the genus. Overall, the matK gene proves useful for describing phylogenetic relationships at the genus level, although its ability to resolve relationships among closely related species remains limited. These findings contribute to a better understanding of the evolutionary structure of Oryza and highlight the potential importance of wild rice species in future conservation and breeding efforts.
POLA PRODUKSI LISTRIK Bacillus licheniformis strain KGEB16 AMOBIL PADA SISTEM MICROBIAL FUEL CELL (MFC) oktriani, fazdkia; Irdawati; Alicia Farma, Siska; Fadilaturahmah
Jurnal Biogenerasi Vol. 11 No. 2 (2026): April - Juni 2026
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/8cvprk22

Abstract

Peningkatan kebutuhan energi berkelanjutan telah menunutut pengembangan Microbial Fuel Cell (MFC) sebagai teknologi alternatif. Penelitian ini bertujuan untuk menganalisis pola produksi listrik Bacillus licheniformis strain KGEB16 yang diamobilisasi pada sistem Microbial Fuel Cell (MFC). Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan perlakuan variasi konsentrasi natrium alginat (2%, 3%, 4%, 5%, dan 6%) serta lima ulangan. Proses amobilisasi sel dilakukan dengan metode entrapment menggunakan natrium alginat, sedangkan pengukuran tegangan listrik dilakukan setiap 2 jam selama periode inkubasi 24 jam. Data yang diperoleh dianalisis menggunakan uji ANOVA dan dilanjutkan dengan uji Duncan (Duncan Multiple Range Test/DMRT) pada taraf signifikansi 5%. Hasil penelitian menunjukkan bahwa pola produksi listrik mengalami peningkatan secara bertahap sejak awal inkubasi hingga mencapai puncak pada jam ke-20, kemudian menurun pada jam ke-22 hingga 24. Tegangan tertinggi diperoleh pada konsentrasi natrium alginat 5%, sedangkan tegangan terendah diperoleh pada konsentrasi 2%. Dengan demikian, dapat disimpulkan bahwa konsentrasi natrium alginat berpengaruh signifikan terhadap pola produksi listrik, dengan konsentrasi optimum sebesar 5% pada sistem MFC.

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