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Jurnal Penelitian Tanaman Industri (Littri)
Published by Pusat Penelitian dan Pengembangan Perkebunan
ISSN : 08538212     EISSN : 25286870     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Jurnal Penelitian Tanaman Industri (JLITTRI) aims to publish primary research articles of current research topics, not simultaneously submitted to nor previously published in other scientific or technical ojournals. General review articles will not be accepted. The journal maintains strict standards of content, presentation,and reviewing. SCOPE The journal will consider primary research papers from any source if they make an original contribution to the experimental or theoretical understanding and application of theories and methodologies of some aspects of agricultural science in Indonesia including: Estate crops; Soil science; Climate science; Agronomy; Plant breeding; Biotechnology; Genetic resources; Plant pathology; Plant physiology; Entomology; Farming system; Postharvest technology; Socio-economic agriculture; Environment; Agricultural extension. The journal publishes Indonesian or English articles. Since the year of 2017, the jurnal is published twice a year in (June and December).
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 504 Documents
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TOLERANSI 60 AKSESI KAPAS TERHADAP CEKAMAN SALINITAS PADA FASE VEGETATIF EMY SULISTYOWATI; SIWI SUMARTINI; ABDURRAKHMAN ABDURRAKHMAN
Jurnal Penelitian Tanaman Industri Vol 16, No 1 (2010): Maret 2010
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v16n1.2010.20-26

Abstract

ABSTRAKEkstensifikasi pengembangan kapas ke luar Jawa berpeluangmenghadapi masalah salinitas, dan untuk memulai program pemuliaanvarietas kapas tahan salinitas diperlukan informasi ketahanan terhadapsalinitas dari koleksi plasma nutfah kapas. Enam puluh aksesi kapas telahdiuji ketahanannya pada tingkat salinitas 10 g/l NaCl dalam rancanganacak lengkap yang diulang tiga kali. Penelitian dilaksanakan diLaboratorium Pemuliaan pada Balai Penelitian Tanaman Tembakau danSerat pada bulan Agustus – Oktober 2007. Pengamatan meliputi jumlah,panjang, dan berat akar, serta panjang dan berat tunas. Hasil penelitianmenunjukkan bahwa parameter-parameter akar dan tunas yang diamatisampai dengan umur 28 HST belum konsisten untuk dijadikan dasar untukmelakukan skrining aksesi-aksesi untuk ketahanan terhadap cekamansalinitas. Terdapat tiga aksesi yang termasuk kategori peka terhadapsalinitas untuk semua parameter yang diamati, yaitu aksesi-aksesi NF-SC1, NF-SL 2, dan Tamcot SP-37. Selain itu juga terdapat 5 aksesi yangsecara stabil menunjukkan toleransi terhadap cekaman salinitas yaituaksesi-aksesi KPX 22, NH 38, Ngwe Chi 1, Dora 11, DP-NF-3, BRI 1,dan DPX 7062-5228.Kata kunci : Gossypium hirsutum, aksesi, toleransi, salinitas, vegetatifTolerance of 60 Cotton Accessions to Salinity Stress atVegetative StageABSTRACTExtension of cotton development program outside Java potentiallyfaces salinity problem, and therefore, in order to start the cotton breedingprogram for saline resistant varieties, it is required accurate information onresistance level of cotton accessions in the germplasm collection. Sixtycotton accessions have been tested for their tolerance in 10 g/l NaCl incomplete randomized design with three replications. The experiment washeld at the Breeding Laboratory of the Indonesian Tobacco and FiberCrops Research Institute from August to October 2007. Observations weremade on the number, length, and weight of root, as well as length andweight of shoot. Experimental result showed that root and shootparameters observed up to 28 DAP were not consistent for screeningcotton accession tolerant to salinity. There are three intolerant accessions,namely NF-SC 1, NF-SL 2, and Tamcot SP-37. In addition, there are fiveaccessions tolerant to salinity namely KPX 22, NH 38, Ngwe Chi 1, Dora11, DP-NF-3, BRI 1, and DPX 7062-5228.Key words: Gossypium hirsutum, accessions, tolerance, salinity, vegetative
ISOLASI FRAGMEN GEN PENYANDI PUTRESIN N-METILTRANSFERASE DAN QUINOLINAT FOSFORIBOSILTRANSFERASE ASAL TEMBAKAU LOKAL TEMANGGUNG (Nicotiana tabacum) SESANTI BASUKI; NURHAJATI AA MATTJIK; SUWARSO SUWARSO; DESTA WIRNAS; SUDARSONO SUDARSONO
Jurnal Penelitian Tanaman Industri Vol 17, No 3 (2011): September 2011
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v17n3.2011.109-117

Abstract

ABSTRAKUpaya untuk menurunkan kandungan nikotin merupakan salah satuprioritas utama penelitian tembakau. Nikotin adalah senyawa alkaloidutama berpotensi dikonversi menjadi senyawa nor-nikotin yang bersifatkarsinogen. Gen PMT sebagai penyandi enzim putresin n-metiltransferase(PMT) dan gen QPT - penyandi enzim quinolinat fosforibosiltransferase(QPT) merupakan dua gen kunci yang berperan penting pada proses bio-sintesis nikotin. Penelitian ini bertujuan untuk mengisolasi potongan genPMT dan QPT asal tembakau lokal Indonesia, mengkarakterisasi danmenganalisis runutan DNA-nya. Tahapan penelitian dimulai dengan me-rancang primer degenerate berdasarkan informasi yang ada di pangkalandata Bank Gen NCBI (National Centre for Biotechnology Information),mengamplifikasi PCR menggunakan templat DNA genomik tembakaulokal cv. Sindoro1, mengklon potongan DNA hasil PCR dan menentukanrunutan DNA-nya. Hasil penelitian menunjukkan dari dua belas pasangprimer degenerate yang dirancang, hanya dua pasang primer yang meng-hasilkan potongan DNA hasil amplifikasi PCR, yaitu pasangan primerPMt-7 (F & R) untuk gen PMT dan primer QPt-3 (F & R) untuk gen QPT.Setelah dilakukan penentuan runutan DNA-nya, amplikon yang didapatdari hasil PCR dengan pasangan primer PMt-7 sebesar 1418 bp, sedangkanuntuk primer QPt-3 sebesar 205 bp. Runutan DNA gen PMT dan gen QPTasal tembakau lokal cv. Sindoro1 mempunyai tingkat kesamaan yang ting-gi dengan gen PMT dan gen QPT asal tembakau lainnya yang ada dipangkalan data Bank Gen NCBI.Kata kunci : Gen PMT, gen QPT, lintasan biosintesis nikotin, perunutanDNA, amplifikasi PCR, primer degenerateABSTRACTIsolation of Genes encoding Putrescine N-Methyl-transferase and Quinolinat Phosphoribosyl transferasederived from Temanggung Tobacco Cultivar (Nicotianatabacum)Reduction of nicotine content is one of the major objective intobacco research. Nicotine is the main alcaloid compound that potentiallycould be converted into a carcinogenic compound (nor-nicotine). The PMTgene encoding putrescine N-methyl transferase (PMT) and the QPT gene -encoding quinolinate phosphoribosyl transferase (QPT) are the two keyenzymes involved in nicotine biosynthesis. The objectives of this researchwere to isolate PMT and QPT gene fragments originated from Indonesianlocal tobacco, to characterize, and to analyze their DNA sequences. Theresearch activities included: degenerate primer design based oninformation available in the GenBank DNA Database NCBI (NationalCentre for Biotechnology Information), PCR amplification usingdegenerate primer and genomic DNA template of a local tobacco cv.Sindoro1, clone the PCR amplified products, and determine their DNAnucleotide sequences. Results of the experiment indicated that from 12degenerate primer pairs synthesized, only two were able to yield positivePCR amplified products. These primer pairs were PMt-7 (F & R primers)for PMT and QPt-3 (F & R primers) for QPT. After DNA sequencing, theamplified DNA product amplified using PMt-7 degenerate primer pairswere 1418 bp, while that using QPt-3 primer pairs were only 205 bp.Nucleotide sequences of PMT or QPT gene fragments originated fromlocal tobacco cv. Sindoro1 showed a high nucleotide sequences identity ascompared to that of the respective genes from other tobacco species thatwere available in the GenBank DNA Database NCBI.Key words: PMT gene, QPT gene, nicotine biosynthetic pathways, DNAsequencing, PCR amplification, degenerate primer
ELIMINASI Potyvirus PENYEBAB PENYAKIT MOSAIK PADA TANAMAN NILAM DENGAN KULTUR MERISTEM APIKAL DAN PERLAKUAN AIR PANAS PADA SETEK BATANG RITA NOVERIZA; GEDE SUASTIKA; SRI HENDRASTUTI HIDAYAT; UTOMO KARTOSUWONDO
Jurnal Penelitian Tanaman Industri Vol 18, No 3 (2012): September 2012
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v18n3.2012.107-114

Abstract

ABSTRAKMinyak nilam merupakan salah satu bahan baku parfum multifungsiyang bernilai tinggi. Budidaya dan pengembangan tanaman nilamterkendala oleh serangan Potyvirus yang menyebabkan penyakit mosaik.Penelitian ini bertujuan untuk mendapatkan benih nilam bebas virusdengan metode kultur meristem apikal dan perlakuan air panas pada setekbatang. Penelitian dilaksanakan mulai Januari sampai Desember 2010 diLaboratorium Virologi Tumbuhan, Institut Pertanian Bogor dan RumahKasa Hama dan Penyakit, Balai Penelitian Tanaman Obat dan Aromatik(Balittro) di Bogor. Bahan tanaman yang digunakan adalah tiga varietasnilam (Sidikalang, Lhokseumawe, Tapak Tuan). Penelitian terdiri atas (1)Eliminasi Potyvirus pada tanaman nilam menggunakan kultur meristemapikal dan (2) Eliminasi Potyvirus pada setek batang nilam denganperlakuan air panas. Percobaan pertama disusun menggunakan rancanganacak lengkap dengan perlakuan 3 varietas nilam dan 2 tipe eksplan(meristem apikal dan batang terminal), dan diulang 10 kali. Parameteryang diamati adalah persentase pertumbuhan, waktu inisiasi, tinggi, danwarna tunas, serta persentase tanaman yang terinfeksi Potyvirus.Percobaan kedua menggunakan air panas pada tiga tingkatan suhu (50, 55,dan 60 o C) dan tingkatan waktu perendaman (10, 20, dan 30 menit).Percobaan disusun menggunakan rancangan acak lengkap dengan 10perlakuan dan 10 ulangan. Tanaman nilam dipelihara selama 8 minggu dandilakukan pengamatan tinggi setek yang tumbuh dan daun yang bergejalamosaik. Hasil penelitian menunjukkan bahwa tanaman nilam, yangdiperbanyak dari kultur meristem apikal ukuran 0,5-1 mm, menghasilkan33,3-99,9% tanaman bebas virus. Perendaman setek batang nilam di dalamair panas pada suhu 50-60 o C selama 10-30 menit tidak dapatmengeliminasi Potyvirus yang menginfeksi ketiga varietas nilam yangdiuji. Setek batang nilam varietas Tapak Tuan dan Lhokseumawe lebihtoleran terhadap air panas dibandingkan Sidikalang tetapi daya tumbuhyasemakin menurun seiring semakin lama waktu perendaman. Teknik kulturmeristem apikal berpotensi untuk menghasilkan setek nilam yang bebasvirus.Kata kunci : kultur meristem apikal, perlakuan air panas, Pogostemoncablin, PotyvirusABSTRACTPatchouli oil produced by patchouli plant is one of multifunctioningperfume’s raw materials and has high economic value. One importantconstraint during its cultivation is infection by Potyvirus causing seriousmosaic disease. This study was conducted to develop a technique toproduce virus-free cutting seeds using apical meristem culture and hotwater treatment on stem cutting. The study was carried out from January toDecember 2010 in Plant Virology Laboratory of Bogor AgriculturalUniversity and Pest and Diseases screen house of Indonesian Medicinaland Aromatic Crops Research Institute (Balittro) in Bogor. Three varietiesof patchouli plant, i.e. Sidikalang, Lhokseumawe, and Tapak Tuan, wereused in this study. The study consisted of (1) Elimination Potyvirus incuttings of patchouli through apical meristem culture and (2) EliminationPotyvirus in stem cuttings of patchouli with hot water treatment. The firstexperiment was arranged using completely randomized design withtreatments of three patchouli varieties and two explant types (apicalmeristem and stem terminal), and it was replicated 10 times. Parametersobserved were bud growth percentage, initiation time, height, and color,and also percentage of plant infected by Potyvirus. The second experimentapplied hot water at three temperature levels (50, 55, and 60 o C) andsubmersion periods (10, 20, and 30 minutes). It was arranged usingrandomized complete design, consisting of 10 treatments with 10 plantsfor each treatment. The patchouli plants were maintained for 8 weeks andobservations were made for height of growing cuttings and leaves withmosaic symptoms. The results showed that the patchouli plants propagatedfrom apical meristem culture of 0.5-1 mm in sizes yielded 33.3-99.9%virus-free plants. Submersion of patchouli stem cutting seeds in hot waterof 50-60 o C and soaking period of 10-30 minutes could not eliminated theinfecting Potyvirus on patchouli the three tested varieties. Cutting seeds ofLhokseumawe and Tapak Tuan varieties were more tolerant to hot waterthan Sidikalang one. However, their ability to grow decreased in line withlonger submersion time period. Apical meristem culture technique ispotential to produce virus-free cutting seeds of patchouli.Key words: apical meristem culture, hot water treatment, Pogostemoncablin, Potyvirus
PENGENDALIAN PENYAKIT LAYU BAKTERI NILAM MENGGUNAKAN PSEUDOMONAD FLUORESEN NASRUN NASRUN; CHRISTANTI CHRISTANTI; TRIWIDODO ARWIYANTO; IKA MARISKA
Jurnal Penelitian Tanaman Industri Vol 11, No 1 (2005): Maret 2005
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v11n1.2005.19-24

Abstract

ABSTRAKPenelitian pengendalian penyakit layu bakteri nilam (Ralstoniasolanacearum) menggunakan pseudomonad fluoresen di kebun petaninilam Desa Situak Pasaman Barat, Sumatera Barat telah dilakukan padabulan Oktober 2003 sampai dengan Juni 2004. Penelitian ini bertujuanuntuk mendapatkan pseudomonad fluoresen yang berpotensi untukmengendalikan penyakit layu bakteri, dan meningkatkan pertumbuhandan produksi nilam. Isolat pseudomonad fluoresen Pf 63, Pf 90, Pf 91, Pf147, dan Pf 180 sebagai perlakuan diisolasi dari rizosfer nilam sehat, dandiseleksi  berdasarkan  kemampuan  antagonistik  terhadap  R.solanacearum secara in vitro di Laboratorium Bakteriologi TumbuhanFakultas Pertanian UGM. Isolat pseudomonad fluoresen tersebutdiintroduksikan ke nilam dan diadaptasikan selama 1 minggu sebelumditanam. Tanaman yang telah diperlakukan dengan isolat pseudomonadfluoresen ditanam pada kebun yang telah terinfeksi dengan patogen padabulan Oktober 2003. Percobaan disusun dalam rancangan acak kelompok(RAK) dengan 6 ulangan. Parameter pengamatan adalah masa inkubasi,intensitas penyakit, pertumbuhan tanaman dan produksi minyak nilam.Hasil penelitian menunjukkan bahwa isolat pseudomonad fluoresendapat mengendalikan penyakit layu bakteri dengan perpanjangan masainkubasi 6-52 hari dan penekanan intensitas penyakit 31,11 – 50,56%.Disamping itu isolat pseudomonad fluoresen dapat mempengaruhipeningkatan pertumbuhan tanaman yaitu tinggi tanaman (6,7 – 26,3 cm),jumlah daun (4,6 – 30,1 daun/tanaman) dan berat kering daun (24,5 –154,3 g/tanaman), dan produksi minyak nilam terutama jumlah minyak(4,8 – 22,3 ml/tanaman). Hasil percobaan ini menunjukkan bahwa isolatPf 91 mempunyai kemampuan antagonistik tertinggi dalam mengen-dalikan penyakit layu bakteri di lapangan.Kata kunci : Nilam, Pogostemon cablin Benth, penyakit layu, bakteri,pengendalian hayati, pseudomonad fluoresenABSTRACTControlling bacterial wilt disease on patchouli plant withfluorescent pseudomonadThe study of controlling bacterial wilt disease on patchouli plant(Ralstonia solanacearum) with fluorescent pseudomonad was carried outin a farmer’s field in Situak Village West Pasaman, West Sumatera fromOctober 2003 to June 2004. The aims of the study were to find out theeffectiveness of fluorescent pseudomonad for controlling bacterial wiltdisease, increasing plant growth and production. Isolates of fluorescentpseudomonad Pf 63, Pf 90, Pf 91, Pf 147 and Pf 180 as treatments wereisolated from the rhizosphere of healthy patchouli plant, and selectedbased on antagonistic activity on R. solanacearum in vitro at theLaboratory of Plant Bacteriology, Faculty of Agriculture, UGM. Theisolates were inoculated on patchouli plant and adapted for one weekbefore planting. The plants treated with fluorescent pseudomonadisolates were planted in the field infected with pathogen on October2003. The experiment was arranged in a randomized block design(RBD) with six replications. The assessment parameters were incubationperiod, disease intensity, plant growth and production of patchouli plants.The results showed that fluorescent pseudomonad isolates could controlthe bacterial wilt disease and delay the incubation period 6-52 days anddecrease the disease intensity 31,11–50,56%. In addition fluorescentpseudomonad isolates could affect the increase of plant growth, i.e. plantheight ( 6,7 – 26,3 cm ), leaf numbers (4,6 – 30,1 leaves/plant) and dryweight of leaves (24,5 – 154,3 g/plant), and plant production, especiallyoil content (4,8 – 22,3 ml/plant). The results of the experiment showedthat Pf 91 isolate had the highest antagonistic activity on controlling thebacterial wilt disease on field.Key words : Patchouli, Pogostemon cablin Benth, wilt disease,bacterial, biological control, fluorescent pseudomonad
RESPON EMPAT KELAPA HIBRIDA HARAPAN TERHADAP PENYAKIT BUSUK PUCUK DAN GUGUR BUAH A. A. LOLONG; E. T. TENDA; J. C. ALOUW
Jurnal Penelitian Tanaman Industri Vol 6, No 1 (2000): Juni, 2000
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v6n1.2000.25-28

Abstract

Response of four new coconut hybrids to bud rot and premature nutfull diseasesThe objective of Ihe experiment was to find out Ihe resistance of four new coconut hybrids GRA x DMT. GKB x DMT, GKN x DTE. GKB x DTE against bud rol and premature nutfall diseases due lo Phytophthora palmivora. The field observation on bud rot was conducted in coconut trial plots at Kima Atas experimental garden. Minahasa District, North Sulawesi Province for 4 years (1995-1998). The field trial was arranged in a randomized block design with 6 treatments (4 coconut hybrids. 2 control) and 60 replicates The experiment under laboratory condilion was conducted to know the artificial infection development of P palmivora on coconut nut The experiment was arranged in a completely randomized design with 6 treatments (4 coconut hybrids, 2 control) and 10 replicates. The result of the field observation showed that percentage of coconut infection by bud rot per cultivar were PB-121 (23.33%) as the highest and then followed by GKN x DTE (8.33%), KHINA-1 (5.0%) and GKB x DMT (3.33%) while cultivars of GKB x DTE and GRA x DMT unlil 4 years observation were not infected by bud rot In laboratory trial, Ihe growth rate of pathogen was very high on KHINA-1 (12.62 mm2/day). The growth rate of pathogen in GKB x DIE and GRA x DMT were slowest (8 9 mm'/day) while those in PB- 121, GKN x Dll and GKB x DMT were moderate (9-10.2 mm2/day).
POTENSI BAKTERI ENDOFIT DALAM UPAYA MENINGKATKAN PERTUMBUHAN, PRODUKSI, DAN KANDUNGAN ANDROGRAFOLID PADA TANAMAN SAMBILOTO GUSMAINI GUSMAINI; SANDRA ARIFIN AZIZ; ABDUL MUNIF; DIDY SOPANDIE; NURLIANI BERMAWIE
Jurnal Penelitian Tanaman Industri Vol 19, No 4 (2013): Desember 2013
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v19n4.2013.167-177

Abstract

ABSTRAKBakteri endofit hidup di dalam jaringan tanaman yang sehat danberperan antara lain di dalam memacu pertumbuhan tanaman denganmenghasilkan senyawa-senyawa zat pengatur tumbuh, seperti IAA, GA 3 ,dan Sitokinin. Tujuan penelitian ini untuk mengevaluasi potensi bakteriendofit dalam  meningkatkan pertumbuhan, produksi, dan kadarandrografolid pada tanaman sambiloto. Penelitian dilakukan di rumah kacaBalittro Cimanggu Bogor pada Oktober 2011–Mei 2012. Perlakuandisusun mengikuti Rancangan Acak Kelompok, enam perlakuan danempat ulangan. Perlakuan terdiri dari (1) kontrol, dan perlakuan bakteriendofit yaitu (2) 20BB, (3) 5MD, (4) 20BD, (5) 20CD (perlakuan 2-5masing-masing terdiri dari 4 jenis isolat), dan (6) 90AA (isolat tunggal).Suspensi bakteri endofit (50 ml/tanaman) diberikan 4 kali yaitu padaminggu ke 3, 5, 7, dan 9 setelah tanam dengan konsentrasi 10 10 spk/ml.Hasil penelitian menunjukkan bahwa bakteri endofit berpengaruh postifdan nyata dalam meningkatkan pertumbuhan, produksi herba segar dankering serta andrografolid pada tanaman sambiloto lebih baikdibandingkan kontrol. Peningkatan pertumbuhan tertinggi ditunjukkanpada tinggi tanaman dan jumlah cabang primer yaitu masing-masing24,7% (20 CD) dan 42,2% (20 BB). Produksi herba kering meningkat 25-82,81%, sejalan dengan meningkatnya serapan hara N (64,7-158,8%), P(50-100%), dan K (65-155%). Peningkatan produksi herba kering danandrografolid terbaik diperoleh dari penggunaan 20 CD (82,81 dan142,11%), 20 BB (88,75 dan 131,58%), dan 20 BD (65,63 dan 131,58%).Implikasi dari hasil penelitian ini bahwa bakteri endofit berpotensi untukdikembangkan pada budidaya tanaman sambiloto.Kata kunci: Andrographis paniculata, bakteri endofit, andrografolid,pertumbuhan, produksiABSTRACTEndophytic bacteria live within healthy plant tissue and playimportant roles, such as producing compounds of plant growth regulatorssubstances such as IAA, GA 3 , and Cytokinin. The aims of this research isto evaluate the potential of endophytic bacteria to promote the growth,andrographolide content, and dry matter yield of king of bitter. Theresearch was conducted in the greenhouse of Cimanggu Balittro in October2011-May 2012. Treatments were arranged in a randomized completeblock design with six treatments and four replications. Treatments consistof (1) control, and 5 kinds of endophytic bacteria isolates such as (2)20BB, (3) 5MD, (4) 20BD, (5) 20CD (treatments no.2-5, consisted of 4types of isolate), and (6) 90AA (single isolate). The highest presentage ofplant height and number of primary branches were obtained from thetreatment of 20CD (24.7%) and 20BB (42.2%). Increase in the dry herbyield of 25-82.81% was in agreement with increasing in uptake of N (64.7-158.8%), P (50-100%), and K (65-155%). The best treatment with whichyielding high of dry herbs and andrographolide was 20CD isolates (82.81and 142.11%), followed with 20 BB (88.75 and 131.58%), and 20 BD(65.63 and 131.58%). The study implies that endophytic bacteria havepotential for development of king of bitter cultivation.Key words: Andrographis paniculata, endophytic bacteria, androgra-pholide, growth, yield
PENGARUH NAA DAN IBA TERHADAP PERAKARAN PURWOCENG (Pimpinella pruatjan Molk.) 77V VITRO SITTIFATMAH SYAHID; OTIH ROSTIANA; MIFTAKHUROHMAH MIFTAKHUROHMAH
Jurnal Penelitian Tanaman Industri Vol 11, No 4 (2005): DESEMBER 2005
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v11n4.2005.146-151

Abstract

ABSTRAKPurwoceng (Pimpinella pruatjan Molk.) merupakan tanaman obatlangka yang cukup potensial untuk dikembangkan sebagai bahan bakuafrodisiak. Untuk mendukung budidaya tanaman ini diperlukan bahantanaman yang memadai. Perbanyakan in vitro purwoceng untukmemperoleh bahan tanaman secara masal masih dibatasi oleh sulitnyamenginduksi akar, yang berakibat rendahnya keberhasilan aklimatisasi dilapangan. Penelitian bertujuan untuk mendapatkan teknik induksiperakaran dengan menggunakan dua jenis auksin (NAA dan IBA) padaberbagai taraf konsentrasi yaitu 0; 0,1; 0,2; 0,4; 0,6; 0,8; 1,0; 1,5 dan 2,0mg/1. Penelitian ini dilaksanakan di Laboratorium Kultur Jaringan, BalaiPenelitian Tanaman Rempah dan Obat, Bogor dari bulan Januari 2003sarnpai dengan Februari 2004. Rancangan percobaan yang digunakanadalah acak lengkap dengan tiga ulangan. Setiap ulangan terdiri dari tigatunas. Parameter yang diamati adalah jumlah akar, panjang akar danjumlah daun layu serta penampakan kultur secara visual. Hasil penelitianmenunjukkan bahwa auksin NAA nyata menghasilkan jumlah akar lebihbanyak dan lebih panjang dari IBA. Penggunaan NAA 0,8 g/1 merupakankonsentrasi terbaik untuk induksi akar. Tidak ada perbedaan yang nyatadari penggunaan NAA atapun IBAterhadap parameter jumlah daun layu.Kata kunci : Purwoceng, Pimpinella pruatjan Molk, tanaman obat,pengatur tuumbuh , NAA, IBA, induksi akar, in vitro, BogorABSTRACTEffect of NAA and IBA on root induction of pruatjan(Pimpinella pruatjan Molk.) in vitroPruatjan (Pimpinella pruatjan Molk.) is one of endangered specieswhich is potential to be developed as aphrodisiac source. To supportpruatjan cultivation , it is needed to prepare the planting material. In vitropropagation of pruatjan is hampered by the difficulty in inducing thenormal roots which affect the successful plant acclimatization. Theobjective of this research was to obtain the root induction technique usingtwo kinds of auxin (NAA and IBA ) at several concentrations i.e : 0; 0.1;0.2; 0.4; 0.6; 0.8; 1.0; 1.5 and 2.0 mg/1. This experiment was conductedfrom January 2003 to February 2004 at the Tissue Culture Laboratory ofIndonesian Spices and Medicinal Crops Research Institute (ISMECRI) inBogor. Experiment was designed as a completely randomized design withthree replications. Each replication consisted of three shoots. Theparameters observed were number of roots, length of roots, number ofsenessence leaves and culture performance. The result showed that NAAproduced the greatest and the longest roots compared to that of DBA. Theuse of NAA 0,8 mg/1 performed the best treatment to induce roots. Thenumber of senessence leaves was neither affected by NAA nor IBA.Key words: Pruatjan, Pimpinella pruatjan Molk., medicinal plant,growth regulator, NAA, IBA, root induction, in vitro
POTENCY OF BLACK TEA FILTRATE AS UV PROTECTANT TO S/NPV JTM 97 C IN CONTROLLING HELICOVERPA ARMIGERA ON SOYBEAN Anella Retna Kumala Sari; Tiara Eka Ariestantia; Fery Abdul Choliq
Jurnal Penelitian Tanaman Industri Vol 26, No 1 (2020): June, 2020
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v26n1.2020.40-48

Abstract

Helicoverpa armigera is the important pest on soybean which  usually controlled by chemical insecticide spraying, so it should be replaced by naturally control using biological agents such as Spodoptera litura Nuclear Polyhedrosis Virus (S/NPV) JTM 97 C. It has some weakness such as during it’s applied in the field the effectiveness can become decreased after exposure the sunlight, especially ultraviolet (UV) so it needs the protective material, such as black tea filtrate which is known containing epigallocatechin gallate, caffeic acid, and apigenin which have role as UV protectors. This research aimed to investigate the potential of black tea filtrate to protect the S/NPV JTM 97 C from UV in controlling of H. armigera. The research was conducted in Pests and Diseases Laboratory on Indonesian Legumes and Tuber Crops Research Institute (ILETRI), Kendalpayak, Malang from January to June 2015. The research used Completely Randomized Design with seven treatments and four replications. They were S/NPV JTM 97 C without UV protectant as control (P1), S/NPV JTM 97 C + kaolin as comparison (P2), S/NPV JTM 97 C + black tea  filtrate concentration 0.5 (P3), 1.0 (P4), 1.5 (P5), 2 (P6), and 2.5% (P7). Results showed that all of the protective materials of black tea filtrate were unable to provide mortality of H. armigera larvae better than kaolin. Kaolin can cause mortality of H. armigera larvae up to 82,50%. All of Black tea filtrate concentrations tested were not able  to be used as  protectant of  S/NPV JTM 97 C due to it can only cause mortality of H. armigera up to 47,50% .  Keywords: Entomopathogenic virus,  LC50, protectant material,  pest.
RESISTENSI BEBERAPA AKSESI WIJEN TERHADAP SERANGAN HAMA TUNGAU (Polyphagotarsonemus latus Banks) S. W . TUKIMIN; R. D. PURWATI; WIDI RUMINI
Jurnal Penelitian Tanaman Industri Vol 15, No 4 (2009): Desember 2009
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v15n4.2009.184-191

Abstract

ABSTRAKSerangan hama tungau Polyphagotarsonemus latus (Banks) padatanaman wijen (Sesamum indicum L.) mulai terjadi pada tahun 2005 yangmengakibatkan penurunan produksi di daerah-daerah pengembangan.Langkah awal untuk mendapatkan varietas tahan P. latus adalahmengetahui sumber ketahanan pada plasma nutfah wijen. Pengujianresistensi beberapa aksesi wijen terhadap hama tungau P. latus dilaksana-kan di Kebun Percobaan Sumberrejo, Bojonegoro, mulai April sampai Juli2008. Tujuan penelitian untuk mengetahui tingkat ketahanan aksesi-aksesiwijen terhadap tungau Polyphagotarsonemus latus (Banks). Perlakuanyang terdiri dari 25 aksesi wijen disusun dalam rancangan acak kelompok,dan diulang tiga kali. Pengamatan meliputi: intensitas kerusakan daun,serta jumlah telur, larva, nimfa dan imago P. latus dan dilakukan mulai 25HST hingga 75 HST dengan interval 10 hari sekali. Hasil penelitianmenunjukkan bahwa intensitas serangan hingga 75 HST terendah padaaksesi HD 11 (50,0%). Nilai tersebut termasuk dalam kategori agak tahanterhadap kerusakan tungau P. latus. Intensitas kerusakan tertinggi padaaksesi HD 3 (63,33%), HD 8 dan HD 15 masing-masing 61,67%, dantermasuk kategori rentan terhadap serangan tungau P. latus. Populasilarva, nimfa, dan imago P. latus berpengaruh terhadap tingginya nilaiintensitas kerusakan.Kata kunci : Sesamum indicum, resistensi, aksesi, Polyphagotarsonemuslatus (Banks)ABSTRACTThe Resistance of Sesame (Sesamum indicum L.)Accessions Against Broad Mite Polyphagotarsonemuslatus (Banks)Since 2005, investation of mite Polyphagotarsonemus latus (Banks)has affected the decrease of sesame (Sesamum indicum L.) productivity inits cultivation area. The initial first improvement to gain resistant varietiesto P. latus was identifying resistant source/gene of sesame germplasm. Theresistance evaluation of sesame accession against mite was conducted inexperimental station of IToFCRI, Sumberrejo, Bojonegoro from Apriluntil July 2008. This experiment was aimed at finding out resistanceaccessions against leaf mite P. latus. The treatment consisted of 25accessions and was arranged in randomized blok design with threereplications. The parameter observed were: leaves damage intensity, eggs,larvae, nymph and adult of P. latus. The observation had been performedsince 25 days after planting (DAP) with ten days interval until 75 DAP.The result showed that the lowest damage intensity at 75 DAP, wasobserved on HD 11 (50,0%). as moderately resistance. Other accessionswith high damage intensity were HD 3 (63,33%), HD 8 and HD 15(61,67%) and were categorized as sensitive to mite P. latus. Population oflarvae, nymph, and adult of P. latus influenced the damage intensity.Key words: Sesamum indicum, resistance, accessions, Polyphagotar-sonemus latus (Banks)
PERTUMBUHAN EMPAT KLON HARAPAN VANILI (Vanilla planifolia) PADA UMUR FISIOLOGIS DAN POSISI RUAS YANG BERBEDA SUKARMAN SUKARMAN
Jurnal Penelitian Tanaman Industri Vol 17, No 1 (2011): Maret 2011
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v17n1.2011.1-5

Abstract

ABSTRAKPenelitian dilakukan di Balai Penelitian Tanaman Rempah dan Obat(Balittro) dan Balai Penelitian Tanaman Rempah dan Aneka TanamanIndustri (Balittri) dari bulan Januari 2006 sampai Desember 2007.Penelitian bertujuan untuk mengevaluasi pengaruh posisi ruas terhadapdaya tumbuh dan pertumbuhan benih empat klon harapan vanili, sebagaisalah satu landasan penetapan standar prosedur operasional (SPO)perbanyakan benih vanili secara vegetatif. Penelitian terdiri atas duakegiatan, yaitu: (1) Pengaruh umur fisiologis dan posisi ruas yang berbedaterhadap daya tumbuh setek dan pertumbuhan vanili klon 1 dan 2, dan (2)Pengaruh posisi ruas terhadap daya tumbuh setek dan pertumbuhanbenih/setek vanili klon 3 dan klon 4. Percobaan pertama disusun dalampola faktorial 2 x 2 x 3 dan dilaksanakan dalam rancangan petak terbagidua kali, dengan 3 ulangan dan 20 setek tiap satuan percobaan. Petakutama adalah dua klon vanili, yaitu klon 1 dan 2, dan anak petak adalah 2umur fisiologis sulur, yaitu (1) sulur umur 6 bulan, dan (2) sulur umur 12bulan setelah pemangkasan. Anak-anak petak adalah setek pada limaposisi ruas, yaitu 1) pertama, 2) kedua, 3) ketiga, 4) keempat, dan 5) ke-lima. Percobaan kedua menggunakan rancangan petak terbagi yangdiulang 3 kali dengan 20 sampel tanaman setiap perlakuan dan ulangan.Petak utama adalah dua nomor klon harapan vanili yaitu klon 3 dan 4,anak petak adalah setek pada 10 posisi ruas, yaitu 1) pertama, 2) kedua, 3)ketiga, 4) keempat, 5) kelima, 6) keenam, 7) ketujuh, 8) kedelapan, 9) ke-sembilan, dan 10) kesepuluh. Posisi ruas yang digunakan dalam keduapercobaan tersebut dihitung dari bagian atas sulur, setelah 2 ruas daripucuk dibuang. Peubah yang diamati meliputi daya tumbuh setek,pertumbuhan tanaman (tinggi tanaman/panjang sulur, jumlah dan panjangruas, serta jumlah dan ukuran daun. Pertumbuhan vanili tidak dipengaruhioleh jenis klon serta umur fisiologis sulur, tetapi dipengaruhi oleh posisiruas. Setek dari ruas kedua sampai ketujuh mempunyai pertumbuhan yangterbaik, dengan daya tumbuh berturut-turut 87,47; 84,58; 81,25; 85,00;81,67; dan 83,83%. Disimpulkan bahwa perbanyakan tanaman vanili dapatmenggunakan setek l ruas yaitu dari ruas kedua sampai ruas ketujuh.Kata kunci: Vanilla planifolia, umur fisiologis, sulur, posisi ruas, dayatumbuh, pertumbuhanABSTRACTGrowth of Four Promissing Vanila Clones (Vanillaplanifolia) at Different Physiological Stages and Inter-nodes PositionsThe experiment was conducted at the Indonesian Medicinal andAromatic Crops Research Institute (IMACRI) and Indonesian Spice andIndustrial Crops Research Institute (ISICRI), from January 2006 untilDecember 2007. This experiment aimed at evaluating the effect ofphysiological stages and internodes positions on the cutting germinationpercentage and growth of four vanilla clones, in terms of establishingstandard operational procedure (SOP) of vegetative propagation of vanilla.The research consisted of 2 experiments, i.e. (1) effect of physiologicalstages and internodes positions on the cutting germination and growth ofvanilla clon 1 and 2, and (2) effect of internodes positions on thegermination and growth of vanilla clone 3 and 4. The first experimenttested 3 treatment factors and was arranged in 2 x 2 x 3 factorial pattern insplit-split plot design with 3 replicates. Twenty cuttings were planted ineach experimental unit. The main plots were two vanilla clones (clone 1and 2), and sub plots were two different physiological stages of internodes,i.e. (1) 6 and (2) 12 months old after cutting their main stem. While thesub-sub plots were cuttings from five internodes positions : (1) first, (2)second, (3) third, (4) fourth, and (5) fifth internodes. The secondexperiment was arranged in factorial split plot design with 3 replicates.The main plots were 2 different vanilla clones, i.e. (1) clone 3 and 4, andsub-plots were cuttings from 10 different internodes positions, i.e. 1) first,(2) second, (3) third, (4) fourth, (5) fifth, 6) sixth, 7) seventh, 8) eighth, 9)ninth, and 10) tenth internodes. Variables observed were germination ofcuttings and plant growth (plant height, number and length of internodes,number and size of leaves). The results indicated that growth of vanillawas not affected by clones and physiological stage of cuttings, but it wassignificantly influenced by internodes positions. Cuttings originated fromsecond until seventh internodes positions resulted in the best growth ofvanilla plant, with germination rates of 84.58; 81.25; 85.00; 81.67; and83.83 %, respectively. It could be concluded that cuttings from seconduntil seventh internodes positions were recommended for plantingmaterials of vanilla.Key words: Vanilla planifolia, physiological stages, internodes positions,germination, growth

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