cover
Contact Name
Dwi Anggorowati Rahayu
Contact Email
dwirahayu@unesa.ac.id
Phone
+6281235407983
Journal Mail Official
jrba@unesa.ac.id
Editorial Address
Department of Biology Faculty of Mathematics and Natural Sciences Universitas Negeri Surabaya Jalan Ketintang Gedung C3 Lantai 2 Surabaya 60231
Location
Kota surabaya,
Jawa timur
INDONESIA
Jurnal Riset Biologi dan Aplikasinya
ISSN : -     EISSN : 26559927     DOI : 10.26740/jrba
Jurnal Riset Biologi dan Aplikasinya is a peer-reviewed journal that strives to provide scientific information on the research results which focused on biological science (Ecology, Animal and Botanical Systematics, Microbiology, Zoology, Botany, Biotechnology, and Genetics and Evolution) and biological education (Innovation of Biology Learning, Assessment and Evaluation in Biology, and Media of Biology)
Articles 125 Documents
Genetic Variation of F2 Progeny Test Acacia auriculiformis A. Cunn. Ex Benth. at KHDTK Blok Playen, Gunungkidul Katon Dewanto, Prasojo; Supriyadi, Supriyadi; Nufus, Malihatun; Siwi Yuliastuti, Dwi
Jurnal Riset Biologi dan Aplikasinya Vol. 7 No. 1 (2025)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v7n1.p99-109

Abstract

Acacia auriculiformis is one of a priority species for forestry in Indonesia. This species has great potential. Breeding of the A. auriculiformis species in KHDTK Blok Playen, Gunungkidul, has reached the second-generation breeding cycle. Further evaluation of the A. auriculiformis progeny test planting in KHDTK Block Playen is needed to develop a certified and more optimal seedling seed orchard.  This study aimed to evaluate the growth of 9-year-old A. auriculiformis progeny test in KHDTK Blok Playen, Gunungkidul. Data collection of tree growth included total height, diameter, branch-free stem height, and stem straightness. Measurements were conducted using the census method on 30 families with four tree plots in 12 blocks of F2 A. auriculiformis progeny test at the KHDTK Blok Playen.  The results of the analysis of variance show that the family source of variation significantly affected the four growth characteristics observed. These results indicate that genetic factors greatly influence the diversity or variation in the measured characters. Strong phenotypic correlation was found between height with diameter character (0,569) and the branch-free stem height with a stem-straightness (0,633). In general, the family and individual heritability values ​​in this study were classified as high. The genetic gain from individual selection (within plot selection) at 25% intensity showed moderate to very high values, ​​ranging from 3.123%-16.494% for the four observed characteristics.
The Inhibitory Effect of East Indonesia Endemic Plant Itchy Leaves on Staphylococcus epidermidis as a Normal Skin Flora Malawat, Siti Rabida Nurhasanah; Warella, Juen Carla; Bandjar, Fitri Kadarsih; Effendi, Effendi
Jurnal Riset Biologi dan Aplikasinya Vol. 7 No. 1 (2025)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v7n1.p66-76

Abstract

Itchy leaf (Laportea decumana) is one of the plants endemic to Eastern Indonesia. People usually use itchy leaves as an alternative treatment for pain and soreness by rubbing it on the surface of the body. Itchy leaves are very useful because they contain abundant secondary metabolites. The application of itchy leaves by rubbing can certainly affect normal skin flora such as Staphylococcus epidermidis, leading to health problems. This study aimed to investigate the effect of methanol extract of itchy leaves on the normal skin flora of S. epidermidis. This test was conducted using the disk diffusion method. The results of this study showed that all concentrations of itchy leaf extract inhibited the growth of S. epidermidis. The highest inhibitory activity was observed at 50% concentration (23.00 ± 7.20), categorized as very strong, followed by 100% concentration (15.00 ± 2.90), 25% (18.00 ± 11.3), 12.5 (13.40 ± 3.51), and 6.25 (10.40 ± 3.10), all categorized as strong. These findings indicate that the prolonged use of itchy leaves may affect the growth of normal flora, resulting in health problems. On the other hand, itchy leaves have the potential to be developed as an antibacterial agent in the future.
Composition of Freshwater Gastropods (Mollusca) in the Midstream of the Cisadane River, West Java, Indonesia Ridwan, Muhammad; Kadri S, Muh.; Reza Sese, Moh.; Iqram, Muhammad; Priawandiputra, Windra
Jurnal Riset Biologi dan Aplikasinya Vol. 7 No. 2 (2025)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v7n2.p118-127

Abstract

Gastropods are important components of freshwater ecosystems, but their diversity and distribution in the Cisadane River, West Java, had not been previously documented. This study investigated the composition and distribution of gastropods at four stations using purposive sampling with 1 × 1 m quadrats. Data were analyzed with the Shannon–Wiener diversity index, Simpson dominance index, Pielou’s evenness index, Kruskal–Wallis test, and ANOSIM to compare community structures among stations, while Spearman’s correlation was applied to examine relationships between species abundance and environmental factors. A total of seven species were recorded: Melanoides tuberculata, Tarebia granifera, Sulcospira testudinaria, Mieniplotia scabra, Clea helena, Radix rubiginosa, and Filopaludina javanica. Station 1 showed the highest diversity (H′ = 1.30) and evenness (E = 0.73), while T. granifera was the most abundant and invasive species. These findings provide the first comprehensive data on freshwater gastropods in the Cisadane River and highlight their potential as bioindicators for conservation and management.
Morphology and Molecular Identification of Cyprinidae from the Sumber Umbulan Ngenep, Malang Regency, East Java Naya Rabbani, Calista; Damayanti, Ayu Elysia; Tresna Sulung, Cahya Ajeng Valenta; Rahayu, M.Si, Dwi Anggorowati; Nugroho, Endik Deni; Rusdianto, Rusdianto; Binti Mamat, Noorhidayah
Jurnal Riset Biologi dan Aplikasinya Vol. 7 No. 2 (2025)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v7n2.p142-159

Abstract

The genus Tor comprises freshwater fish belonging to the Cyprinidae family, recognized for their remarkable diversity and broad distribution across Southeast Asia, including Indonesia. Despite this, data on Tor species in certain regions are limited, highlighting the need for additional research to confirm their presence. Therefore, this study aims to examine the genetic diversity and phylogenetic reconstruction of the species observed in Sumber Umbulan Ngenep using the COI gene as a DNA barcode marker. The research was conducting using the Purposive Random Sampling method and fin tissues were preserved in 96% ethanol for DNA analysis. The species found at Sumber Umbulan Ngenep were observed morphologically using a phenetic approach. Fins from each fish sample were collected and subjected to PCR, sequence analysis using the BOLD System, phylogenetic tree reconstruction, ABGD analysis, and QR barcoding. The results of morphological, genetic, and phylogenetic tree reconstruction based on the COI gene indicate that three species have been identified at Sumber Umbulan Ngenep, namely Tor douronensis, Tor tambra, and Barbonymus gonionotus. Based on the phenetic approach, three apomorphies and five automorphies were identified, indicating the relatedness of the observed species with the reference species. The phylogenetic tree topology shows an unambiguous branching pattern for the observed sample cluster with Poecilia reticulata as the outgroup. This study is the first to identify the species present at Sumber Umbulan Ngenep, Malang Regency, East Java, Indonesia. The findings provide new information about the existence of these species and can serve as a basis for future conservation and management strategies.
Carbon Stock Analysis of Family Fabaceae in the Forest Area of Surabaya State University as An Effort to Conserve the Environment Rohmah, Tri Lestari Saidha; Fida Rachmadiarti
Jurnal Riset Biologi dan Aplikasinya Vol. 7 No. 2 (2025)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v7n2.p128-141

Abstract

Forest vegetation plays a crucial role in mitigating global climate change through carbon sequestration. The Universitas Negeri Surabaya campus forest has significant potential as a carbon sink, particularly through tree species belonging to the Fabaceae family. This study aimed to analyze carbon stocks in Fabaceae trees within the campus forest as an effort toward environmental conservation. Carbon stock estimation was carried out using a non-destructive approach through allometric equations, focusing only on above-ground biomass. The research was conducted in the campus forest area targeting Fabaceae species. Parameters measured included tree trunk diameter at 1.3 meters above ground level, secondary data on wood specific gravity, and leaf chlorophyll content. Data analysis was performed using the Pearson correlation test to determine the relationship between morphological and physiological characteristics of trees and their stored carbon stock. The results showed that the Unesa campus forest contained 4 species and 52 individual trees. The average carbon stock per tree was 213.96 kg C tree⁻¹ year⁻¹, and the total carbon stock in the observed area reached 992.79 kg/ha. Pearson correlation results indicated a positive relationship between morphological and physiological characteristics and carbon stock (Sig. 0.31). Based on these findings, Fabaceae species are recommended as effective contributors to environmental conservation strategies to reduce carbon emissions. These trees also support campus greening programs and align with the achievement of Sustainable Development Goals (SDGs).

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