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All Journal Publikasi Pendidikan Folia Medica Indonesiana Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat BIOMA INKUIRI : Jurnal Pendidikan IPA JMM (Jurnal Masyarakat Mandiri) Jurnal Inovasi Hasil Pengabdian Masyarakat (JIPEMAS) JPBIO (Jurnal Pendidikan Biologi) Jurnal Kesehatan Terpadu (Integrated Health Journal) Biopendix: Jurnal Biologi, Pendidikan dan Terapan Jurnal Riset Biologi dan Aplikasinya Nusantara Science and Technology Proceedings Darmabakti : Junal Pengabdian dan Pemberdayaan Masyarakat MAHESA : Malahayati Health Student Journal BIOEDUPAT: Pattimura Journal of Biology and Learning Molucca Medica Jurnal Pengabdian Masyarakat : Pemberdayaan, Inovasi dan Perubahan PAMERI : Pattimura Medical Review Scientica: Jurnal Ilmiah Sains dan Teknologi Biosaintifika: Journal of Biology & Biology Education
Warella, Juen Carla
Department Of Microbiology And Parasitology, Faculty Of Medicine, Universitas Pattimura, Ambon, Indonesia
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LAMA FERMENTASI TERHADAP KADAR SERAT NATA BUAH GANDARIA Juen Carla Warella; Pamella Mercy Papilaya; Prelly Tuapattinaya
BIOPENDIX Vol 3 No 1 (2016): Biopendix : Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol3issue1page33-39

Abstract

Background: Gandaria fruit on the island of Ambon has benefits if the sale value can be used as raw material for the manufacture of Nata products. The making of Nata Gandaria fruit with the help of Acetobacter xylinum microorganisms, the role of microbes to break down glucose into cellulose or fiber that is important for the human body. Methods: To know the length of fermentation to know the effect of fiber content of Gandaria nata, then tested on fiber content of nata Gandaria fruit that has been fermented for 10 days and 12 days. Result: Based on the test results using gravimetry fiber content method known nata fermentation time can increase fiber content. The highest increasing fiber content at nata occurred on day 12 with an average fiber content of 0.45% and the lowest occurred on fermentation day 10 with an average fiber content of 0.35%. Conclusion: The duration of fermentation has an effect on the fiber content of gandaria fruit (Bouea macrophylla griff). The duration of fermentation that produces gandaria fruit nata (Bouea macrophylla griff) with the highest fiber content is on the 12th day while the fermentation time that produces the gandaria fruit (Bouea macrophylla griff) with the least fiber content is the 10th day
Organoleptic Test of Eco-enzyme : Fermentation of Banana Peel Waste Juen Carla Warella
Bioma : Jurnal Ilmiah Biologi Vol 12, No 1: April 2023
Publisher : Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/bioma.v12i1.14803

Abstract

Prevention of environmental damage can be done by recycling household waste both organic and inorganic. One of them is by making eco-enzymes made from banana peel waste which is found in the environment. This study aims to determine the level of respondents' liking based on organoleptic test variables. This research method is an experiment that includes making eco-enzymes, and organoleptic tests consisting of aroma, color and texture variables. Data analysis was carried out in a qualitative descriptive way by looking at the level of respondents' liking. The results showed that the average respondent chose a brown color with a percentage of 90%, yellow 7%, and colorless 3%. The scent variable respondents chose sour odor by 70% and other aromas 30%. While the texture of all respondents chose the composition of liquid eco-enzyme. This is due to the metabolic activity of microorganisms that result in the breakdown of substrates by bacteria resulting in changes in the aroma, color and texture of banana peels. The conclusion of this study is that the use of banana peel as the basic ingredient for making eco-enzyme affects the respondents' level of liking. Eco-enzyme can be used as a natural fertilizer for plant growth and can reduce household waste.
Antifungal Activity of Selaginella plana (Desv. ex Poir.) Hieron Extract Against Candida albicans In Vitro Juen Carla Warella; Khairunnida Rahma; Agung Dwi Wahyu Widodo; Rebekah Juniati Setiabudi
Folia Medica Indonesiana Vol. 59 No. 3 (2023): September
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/fmi.v59i3.44165

Abstract

Highlights: 1. A study on the medical benefits of Selaginella plana has significant academic value due to its extensive traditional usage among the Moluccan people as a medicinal remedy, especially for its antifungal properties.2. The findings of this study will allow further screening to determine the mechanism of action of bioactive compounds in inhibiting the growth of Candida albicans   Abstract Candidiasis is an opportunistic infection caused by Candida albicans. This infection commonly affects the skin, oral mucosa, vagina, and gastrointestinal tract. Excessive use of azole antifungals in the treatment of Candida albicans infections can lead to the development of resistance. Therefore, it is necessary to explore alternative treatments using medicinal plants such as Selaginella plana, commonly referred to as “rutu-rutu” in a local language spoken across Maluku, Indonesia. Selaginella plana contains active compounds belonging to various chemical classes, including terpenoids, steroids, flavonoids, and saponins. This study aimed to determine the ability of Selaginella plana extract as an antifungal agent against Candida albicans by evaluating its inhibitory and antifungal effects. This study used an actual experimental design and broth dilution method. The research methodology involved the extraction of Selaginella plana using a solvent of 96% ethanol. The extract was then prepared in various concentrations, i.e., 100%, 50%, 25%, 12.5%, 6.25%, and 3.125%. Additionally, ketoconazole and distilled water were included in the experiment for the positive and negative controls, respectively. The results of this study demonstrated that Selaginella plana extract inhibited the growth of Candida albicans when administered starting at a concentration of 12.5%. However, the antifungal potential of Selaginella plana extract that induced cell death was only observed at a concentration of 100%. The fungicidal activity was exclusively identified in the undiluted, pure extract. The inhibitory and cytotoxic effects of Selaginella plana on Candida albicans cells were attributed to the presence of bioactive compounds in Selaginella plana, including flavonoids, tannins, terpenoids, and saponins. These bioactive compounds had the ability to inhibit cell growth by altering membrane permeability, causing mitochondrial dysfunction, and disrupting ergosterol biosynthesis. It can be concluded that Selaginella plana extract can act as a fungistatic agent against the proliferation of Candida albicans.
Effect of calcium carbide on the rate of ripening of Ambon Bananas (Musa paradisiaca Var. Sapientum (L). Kunt) Jeyssen Lincon Loupatty; Gloria Eka Sari Tuhumena; Gysella Velove Limba; Ayu Wariunsore; Zipora V Noya Van Delzen; Juen Carla Warella; Gerce Anne Rumahlatu
BIOEDUPAT: Pattimura Journal of Biology and Learning Vol 3 No 2 (2023): Bioedupat : Pattimura Journal of Biology and Learning
Publisher : Program Studi Pendidikan Biologi, Pascasarjana, Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/bioedupat.v3.i2.pp118-124

Abstract

Bananas are one of the most widely consumed sources of nutrients in Indonesia. Generally, bananas are consumed fresh and processed into various snacks. This leads to an increased demand for bananas in the market. However, the natural ripening process takes 7-8 days after harvest, which is a long time to meet consumer demand. One solution is to use calcium carbide to accelerate fruit ripening. The reason for using calcium carbide is that it is readily available and inexpensive, so banana producers use carbide as a fruit ripening agent. The objective of this study was to determine the effects of calcium carbide on fruit ripening of Ambon bananas. The method used was a laboratory experiment with a post-test-only control group design. There were three treatments with calcium carbide concentrations of 12%, 6%, and 3%, and positive control, namely rapining with rice. Data were analyzed descriptive qualitative by collecting, presenting, reducing, and conclusions. The process of fruit ripening can be observed using the variables of color, texture and aroma. The results obtained were differences in color, texture, and aroma at concentrations of 12%, 6%, and 3% based on the length of aging. Based on the color parameter, the concentration of 6% and 12% showed a blackish yellow/brown color, while the concentration of 3% was yellow with black spots. The texture parameters of the 6% and 12% concentrations had a very soft texture, while the 3% concentration had a smooth texture. The aroma parameters of the 12%, 6%, and 3% concentrations had a rancid aroma on day six, while days one, two, and three were fragrant. The conclusion is that using calcium carbide at different concentrations affects the rate of fruit ripening. The higher the concentration of calcium carbide used, the faster the respiration rate of the bananas, thus initiating the ripening process
KEJADIAN GASTROESOPHAGEAL REFLUX DISEASE (GERD) BERDASARKAN SKOR GERD-Q PADA MAHASISWA FAKULTAS KEDOKTERAN UNIVERSITAS PATTIMURA TAHUN 2023 Nurul Fadila Rahmadani Maradjabessy; Indrawanti Kusadhiani; Juen Carla Warella
PAMERI Vol 5 No 2 (2023): PAMERI: Pattimura Medical Review
Publisher : Fakultas Kedokteran Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/pamerivol5issue2page76-84

Abstract

Gastroesophageal Reflux Disease (GERD) is a pathological condition due to backflow from the stomach into the esophagus, causing symptoms such as heartburn and regurgitation. Risk factors for GERD are mostly related to a person's lifestyle such as obesity, diet, smoking and stress. One group of individuals who have a high risk is students of the Faculty of Medicine in terms of a series of study loads that make students unable to maintain a good lifestyle. The purpose of this study was to determine the incidence and characteristics of Pattimura University Faculty of Medicine students who experience GERD based on the GERD-Q score. This research is a quantitative research with categorical descriptive study using cross sectional approach. The results showed that the prevalence of GERD was 40 people (15.2%) out of a total of 264 respondents. The majority were female 31 people (11.7%). The highest age was 18 years old, 16 people (6.1%). The most common symptom of GERD is regurgitation as many as 40 people (8.5%). The highest BMI in the normal group was 16 people (6.1%). The conclusion of this study showed that the prevalence of GERD in students of the Faculty of Medicine, Pattimura University was 40 people with the main complaint of regurgitation and the characteristics of respondents with GERD were mostly at the age of 18 years, female, and normal BMI group.
PEMBERDAYAAN MASYARAKAT MELALUI EDUKASI MANFAAT PROBIOTIK DAN PELATIHAN PEMBUATAN MINUMAN TEH KOMBUCHA Melda Yunita; Juen Carla Warella; Eka Astuty; Elpira Asmin; Morgan Ohiwal
JMM (Jurnal Masyarakat Mandiri) Vol 8, No 2 (2024): April
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jmm.v8i2.21658

Abstract

Abstrak: Teh Kombucha merupakan salah satu minuman probiotik yang memiliki manfaat bagi Kesehatan karena mengandung berbagai jenis vitamin, antibakteri, dan antioksidan. Negeri Lonthoir merupakan salah satu daerah yang masyarakatnya belum mengetahui manfaat teh Kombucha sebagai minuman probiotik yang berguna bagi Kesehatan. Kegiatan ini bertujuan untuk meningkatkan pengetahuan masyarakat mengenai manfaat probiotik dan meningkatkan keahlian dalam membuat teh Kombucha. Kegiatan dilakukan pada 27 September 2023 dengan melibatkan 18 anggota masyarakat. Tahapan kegiatan dimulai dengan memberukan pretest, edukasi secara lisan, posttest dan demonstrasi. Hasil analisis menunjukkan terjadi peningkatan pengetahuan sebesar 71,33%. Disimpulkan bahwa kegiatan ini memberikan dampak yang sangat berarti bagi masyarakat dalam meningkatkan pengetahuan dan menambah softskill dalam membuat teh Kombucha secara mandiri.Abstract: Kombucha tea is a probiotic beverage with health benefits because it contains various types of vitamins, antibacterial agents, and antioxidants. Lonthoir is one of the areas where people need to learn the health benefits of kombucha tea as a probiotic beverage. This activity aimed to increase the community knowledge about the benefits of probiotics and improve their skills in making Kombucha tea. The activity was conducted on September 27, 2023, with 18 community members participating. The phases of the activity began with the administration of a pretest, oral education, posttest, and demonstration. The results of the analysis showed a 71.33% increase in knowledge. It was concluded that this activity had a significant impact on the community in terms of increasing knowledge and adding soft skills in making kombucha tea independently.
PERTUMBUHAN BIOFILM ESCHERICHIA COLI PADA MEDIA TRYPTIC SOY BROTH Rahma, Khairunnida; Warella, Juen Carla
Scientica: Jurnal Ilmiah Sains dan Teknologi Vol. 2 No. 4 (2024): Scientica: Jurnal Ilmiah Sains dan Teknologi
Publisher : Komunitas Menulis dan Meneliti (Kolibi)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.572349/scientica.v2i4.1214

Abstract

Mikroorganisme mengembangkan berbagai tipe mekanisme pertahanan yang berbeda-beda. Salah satunya adalah pembentukan biofilm. Biofilm merupakan kumpulan dari mikroorganisme terkait permukaan yang heterogen terselubuh di dalam matriks polimer yang diproduksi sendiri yang terdiri dari polisakarida, protein, dan DNA. Tujuan penelitian ini adalah untuk menganalisis pertumbuhan biofilm bakteri Gram-negatif Escherichia coli pada media Tryptic Soy Broth. Metode yang digunakan adalah microtiter-plate 96 wells. Dilakukan sebanyak enam kali replikasi dan pembacaan Optical Density (OD) menggunakan ELISA reader pada Panjang gelombang 570 nm. Hasil penelitian didapatkan rerata OD 2,810 dengan standar deviasi 0,402. Sehingga dapat ditarik kesimpulan, Escherichia coli termasuk ke dalam kategori bakteri pembentuk biofilm yang kuat.
Pembuatan media pembelajaran kreatif berbasis aplikasi canva pada Guru SD Warella, Juen Carla; Jaya, Gede Wiratma
Jurnal Inovasi Hasil Pengabdian Masyarakat (JIPEMAS) Vol 7 No 3 (2024): Jurnal Inovasi Hasil Pengabdian Masyarakat (JIPEMAS)
Publisher : University of Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/jipemas.v7i3.21146

Abstract

Pendidikan yang berkualitas merupakan kunci untuk menyiapkan sumber daya manusia yang unggul dan siap bersaing menghadapi perubahan zaman. Kualitas pendidikan dapat meningkat jika memiliki tenaga pendidik yang dapat memanfaatkan dan mengoperasikan teknologi informasi dengan baik. Salah satu sarana teknologi informasi yang dapat dimanfaatkan dalam membuat media pembelajaran yaitu aplikasi Canva. Tujuan dari kegiatan ini adalah memberikan pelatihan pembuatan media pembelajaran yang kreatif berbasis aplikasi Canva untuk mengatasi kesulitan yang dialami oleh guru Sekolah Dasar Negeri 4 Halong, Kota Ambon. Metode kegiatan pengabdian kepada masyarakat (PKM) menggunakan metode Service Learning yang terdiri dari tiga tahap, yaitu perencanaan, pelaksanaan, dan evaluasi. Hasil kegiatan pelatihan menunjukkan para guru sangat antusias terhadap pelatihan Canva yang telah diberikan oleh tim PkM sehingga kegiatan ini dapat berjalan dengan baik dan menghasilkan hasil yang nyata dalam mendesain media pembelajaran. Berdasarkan hasil evaluasi kuesioner menunjukkan para guru sangat setuju kegiatan pelatihan Canva telah menambah pengetahuan terhadap aplikasi Canva, meningkatkan kreativitas pembuatan media pembelajaran, dan meningkatkan profesionalisme guru. Pada pelatihan ini perbandingan peningkatan pengetahuan awal tidak diukur sehingga disarankan untuk perlu dilakukan pemberian pretest sebagai acuan mengukur tingkat pengetahuan guru. Pelatihan ini diharapkan dapat membuat proses pembelajaran di kelas menjadi lebih menyenangkan dan interaktif.
Peningkatan Level Pengetahuan Masyarakat Mengenai Manfaat Pala Sebagai Tanaman Rempah Obat (TARO) Yunita, Melda; Ohiwal, Morgan; Alimudi, Saiful; Astuty, Eka; Mus, Rosdiana; Abbas, Muthmainnah; Makatita, Miftah H; Warella, Juen C
Darmabakti : Jurnal Pengabdian dan Pemberdayaan Masyarakat Vol 5 No 01 (2024): Darmabakti : Junal Pengabdian dan Pemberdayaan Masyarakat
Publisher : Lembaga Peneliian dan Pengabdian Masyarakat (LPPM) Universitas Islam Madura (UIM)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31102/darmabakti.2024.5.01.27-34

Abstract

Tanaman pala (Myristica fragrans Houtt) memiliki nilai ekonomis yang tinggi dan multiguna. Selain digunakan sebagai bumbu masak, pala juga memiliki banyak manfaat dalam bidang kesehatan sehingga dapat dijadikan sebagai tanaman rempah obat (TARO). Namun, pemanfaatan pala sebagai tanaman obat belum begitu maksimal di Dusun Air Sakula Negeri Laha Ambon. Padahal, kebanyakan masyarakat disana bekerja sebagai petani pala. Tujuan dari kegiatan ini adalah untuk menganalisis level pengetahuan masyarakat mengenai pemanfaatan tanaman pala sebagai tanaman rempah obat. Kegiatan dilaksanakan pada tanggal 13 Agustus 2022 di Dusun Air Sakula Negeri Laha Ambon dengan melibatkan 20 responden. Tahapan yang dilakukan ialah melaksanakan pretest, penyuluhan TARO, dan posttest serta evaluasi. Hasil analisis menunjukkan bahwa level pengetahuan masyarakat meningkat sebesar 67% setelah mendapatkan penyuluhan. Dapat disimpulkan penyuluhan yang diberikan berdampak positif dan signifikan dalam meningkatkan level pengetahuan masyarakat mengenai pala sebagai tanaman rempah obat.
Effectiveness of Tuba Root (Derris elliptica) on Histological Structure of Rabbit Fish Liver Sekewael, Usi; Leiwakabessy, Fredy; Warella, Juen Carla; Rahaweman, Ayu Christien; Rumahlatu, Dominggus
Biosaintifika: Journal of Biology & Biology Education Vol. 16 No. 2 (2024): August 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v16i2.6754

Abstract

Rabbit fish (Siganus vermiculatus) is one of the biological resources in the sea that is widely consumed by the people in Maluku. Rabbit fish are caught during low tide with nets or by using natural resources to poison the fish, such as tuba root (Derris elliptica), which contains rotenone to make fish easy to catch. This study aimed to determining the concentration of tuba root extract that was effective against damage to the liver tissue of rabbit fish. Rabbit fish and tuba root were collected from Oma Village, Haruku Island, Central Maluku District, Maluku Province.  In the study, a laboratory experiment was conducted in which the tuba root was treated with doses of 0.1 g, 0.3 g, 0.5 g and 1 g with an exposure time of 18 to 24 hours. Data were analyzed using two-way analysis of variance (ANOVA) followed by Duncan's post-hoc test. As a result, the lowest level of liver damage in rabbit fish was observed at a dose of 0.1 g, with a total damage of 7 ± 0.00 and 8.5 ± 0.71. Conversely, the highest level of liver damage was observed at a dose of 1 g, with a total damage of 14 ± 0.00 and 15 ± 0.00. The results of the post hoc test showed a subset value of 0.1 g dose (7.75), 0.3 g dose (9.25), 0.5 g dose (12.00), and 1 g dose (14.50). This research indicates that administering tuba root to rabbit fish can result in damage to the liver's structure. The severity of this damage is contingent upon the dose administered, whereas exposure duration does not affect the level of damage to fish organs.
Co-Authors Abbas, Muthmainnah Agung Dwi Wahyu Widodo Alimudi, Saiful ASTUTI, EKA Astuty, Eka Augie Desvie Klausya Kadun Ayu Wariunsore B. Bension, Johan Bandjar, Fitri Kadarsih Bandjar, Fitri Kardasih Barus, Carolina Sri Athena Christiana Rialine Titaley Cokorda Istri Arintha Devi de Lima, Filda V.I. de Lima, Filda Vionita Irene Dominggus Rumahlatu Effendi Effendi Eka Astuty Eka Astuty Elpira Asmin Elpira Asmin Etrin Zulqarnain Eka Saputra Linggar Fadila Tri Starlia Farah Christina Noya Ferymon Mahulette, Ferymon Filda de Lima Fredy Leiwakabessy Gede Wiratma Jaya Gerce Anne Rumahlatu Gloria Eka Sari Tuhumena Grace Latuheru Gysella Velove Limba Hursepuny, Valentine Husein, Anggun Lestary Indrawanti Kusadhiani Jeyssen Lincon Loupatty Josepina Mainase Kailola, Nathalie E. Kamoda, Tri Puspa Rini Dusung Khairunnida Rahma Latuheru, Grace Lesbatta, Kezia Josawel Makatita, Miftah H Malawat, Siti Rabida Nurhasanah Maxwell Landri Vers Malakauseya Melda Yunita Mus, Rosdiana Noviyanter Siahaya Novy Gaspersz Nurul Fadila Rahmadani Maradjabessy Ohiwal, Morgan Pamella Mercy Papilaya Patteilohy, Merry Prelly Tuapattinaya Rachmawati Dwi Agustin Rahawarin, Halidah Rahaweman, Ayu Christien Ratusehaka, Nopri Rebekah Setiabudi, Rebekah Sekewael, Usi Simal, Rufiati Soumena, Rifah Zafarani Sulfiana Sulfiana SULFIANA, SULFIANA Tahitu, Ritha Tuapattinaya, Prelly M Tuapattinaya, Prelly Marsell Jolanda Veren Wendy Warella Zipora V Noya Van Delzen