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Dwi Anggorowati Rahayu
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Jurnal Riset Biologi dan Aplikasinya
Published by Universitas Negeri Surabaya
ISSN : -     EISSN : 26559927     DOI : 10.26740/jrba
Core Subject : Health, Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Medicine & Pharmacology
Jurnal Riset Biologi dan Aplikasinya is a peer-reviewed journal that strives to provide scientific information on the research results which focused on biological science (Ecology, Animal and Botanical Systematics, Microbiology, Zoology, Botany, Biotechnology, and Genetics and Evolution) and biological education (Innovation of Biology Learning, Assessment and Evaluation in Biology, and Media of Biology)
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ecology, Evolution, Behavior and Systematics
Articles 126 Documents
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On the abundance and occurrence of the mangrove crabs, Scylla spp. (Crustacea: Portunidae) from Munjang mangrove, Bangka Belitung Island Raniah, Nadhifah; Henri, Henri; Kurniawan, Kurniawan
Jurnal Riset Biologi dan Aplikasinya Vol. 4 No. 2 (2022)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v4n2.p75-82

Abstract

The mangrove ecosystem is one of the most productive ecosystems on earth. This ecosystem provides a lot of functions for the living organism inhabiting here, such as fishes, mollusks, echinoderms, crustaceans, ect. The mangrove crab is one of the common crustaceans that can be found in the mangrove. This Purtunidae crab is very popular due to their role in the food chain as well as for the economic income that has a high value in the market. However, overexploitation may decrease the crab population. This study aimed to investigate the abundance and occurrence of the mangrove crabs from Munjang mangrove, Bangka Belitung Island from August 2020 to June 2021 using baited traps with the line transect method at four zones. A total of four species of mangrove crabs, Scylla olivacea, S. paramamosain, S. serrata and S. tranquebarica, were found in this location and dominated by S. serrata. Shannon diversity index (H’) was a in low category (<1), evenness index range (J) from 0.35 to 0.89, and there was dominant species in this study. The bed sediment iswas dominated by the muddy clay substratum except in zone 4 with sandy. Overall, this location is a suitable habitat for the Scylla spp. And better management is urgently needed before these crabs are exploited by humans.
An Additional Information of Tarenna (Rubiaceae) in Madura Island Indah, Novita; Bagus Setiawan, Ashari
Jurnal Riset Biologi dan Aplikasinya Vol. 4 No. 2 (2022)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v4n2.p90-95

Abstract

Tarenna is a genus of relatives of Ixora which is found in forests. Tarenna is different from Ixora which is known as an ornamental plant. The purpose of this study was to find the genus Tarenna in Madura natural habitat to preserved or conserved before this genus became extinct. Observations were made on morphological characters. The morphological characteristics observed included: stature, stems, leaves, inflorescences, flowers, fruits, and seeds. Ixora has a corolla of various flowers while Tarenna only has a white corolla but smells good. Recent exploration and collection of Tarenna in Madura Island indicated that two species are a new record for Java and Madura Island, namely T. costata and T. fragrans. Existence of T. costata in this area not only as a new record for Madura Island, but also as a new record for Java. Tarenna fragrans was firstly reported in Madura Island as a new distributional record for this area. An updated of identification key, several descriptions, and documentations, as well as a distributional map are provided.
Sex Determination of Sea Cucumber Acaudina rosettis from Madura Straits, Indonesia Winarni, Dwi; Lestari, Lita I.D.; Hayati, Alfiah; Nabilla, Nithasya; Khaleyla, Firas
Jurnal Riset Biologi dan Aplikasinya Vol. 4 No. 2 (2022)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v4n2.p83-89

Abstract

Acaudina rosettis is one of the commercial sea cucumber species that is widely consumed on the east coast of Surabaya, East Java, Indonesia. Although its abundance in nature has begun to show signs of decreasing, studies on this species are still very limited. This study aimed to examine indicators that can be used to determine the sex of A. rosettis individuals. Acaudina rosettis samples were collected from Madura strait in the mid of Hijri month for three months. The sea cucumber samples were then dissected. Body wall without viscera and gonads was weighed. The gonads were then characterized macroscopically and microscopically, prepared into histological slides with hematoxylin-eosin staining. The results showed that A. rosettis samples could be classified into three sex groups based on the type of gamete cells observed in the tubules; male (spermatogenic), female (oogenic), and undetermined sex (no gamete cells found). Gonads had three colors; yellow, pink, and orange and were in five stages of reproductive phase; gametogenesis (G), mature (M), spawning (S), and post-spawning (PS). Male samples had yellow and orange gonads, female samples were pink and orange, and all undetermined sex were orange. The G gonads were yellow and orange, M were yellow and pink, while S and PS were orange. Body wall and gonad weight at the same reproductive phase did not show any difference between males and females. Based on the results, gonad color can be used as an indicator of sex determination, but only in G and M reproductive phases.
The Effect of Water Concentration on Growth Media on Lipid Production by Oleaginous Fungi Isolate BR 2.2 Lesti, Herin Yoga; Ilmi, Miftahul
Jurnal Riset Biologi dan Aplikasinya Vol. 4 No. 2 (2022)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v4n2.p51-56

Abstract

Oleaginous fungi are one of the microorganisms that can accumulate a high number of biomasses quickly (within 96-130 hours) and are often used to produce lipids. The growth of fungi depends on the chemical composition of the environment in which it grows. The growth media of fungi must contain high carbohydrates as a source of nutrients and high nitrogen content. One of the carbon sources that fungi can use in the growth process is glucose. BR 2.2 isolate is an oleaginous fungus capable of accumulating 28.44% lipids from the total dry biomass with glucose as a carbon source in 50 mL of growth media. Therefore, this study was conducted to determine the effect of variations in the volume of media and incubation time on the production of biomass and lipid isolate BR 2.2. Biomass and lipid production were analyzed at media with additional water volumes of 10, 20, 30, 40, and 50 mL with 48, 96, and 144 hours of incubation times. The results showed that lipid accumulation and biomass production increased with the reduction of water content in the growth media and reached the highest number in the media volume of 20 mL with an incubation time of 144 hours, i.e., 0.87±0.04 g/L and 12.53±0.29 g/L. It can be concluded that biomass and fungal lipid increased along with incubation time and nutrient concentration.
Filicinae Taxonomic Diversity in the Tourism Area of Tretes Waterfall Wonosalam Kabupaten Jombang Nuraini, Afifah Mei; Wisanti, Wisanti
Jurnal Riset Biologi dan Aplikasinya Vol. 4 No. 2 (2022)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v4n2.p57-68

Abstract

Filicinae is a fern with the most species members. The Filicinae includes approximately 170 genera and 7000 species. Filicinae grows in wet, humid, and shady environmental conditions, like waterfall areas with an altitude of 1100-1300 meters above sea level (m a s l). The study aimed to determine the taxonomic diversity of the Filicinae in the Tretes Wonosalam Waterfall Tourism area. Taxonomic diversity is very important in tourism areas because it prevents species extinction due to the destruction of the habitat, they live in. This was descriptive research with exploration and observation techniques used purposive sampling methods based on altitude. The results of the study were stated in the form of an inventory list of species and families of each station. Data gained of species diversity were analyzed using taxonomic diversity with the formula (?) and dominance diversity with the formula (?*). Exploration results found 37 species from 12 families composed of 10 species from 7 families at station 1; 12 species from 6 families at station 2; 14 species from 7 families at station 3; and 22 species from 12 families at station 4. The highest taxonomic diversity (?) was station 4 with 106.8 while the lowest for station 1 was 25.63. Dominance diversity (?*) at all stations in this study was in the medium category. The highest family found in the study was Tectariaceae with six species.
DNA Barcode of Homalomena pexa inferred from Internal Transcribed Spacer Region Aprilianingsih, Risqi; Farhatul Wahidah, Baiq; Hariri, Muhammad Rifqi
Jurnal Riset Biologi dan Aplikasinya Vol. 4 No. 2 (2022)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v4n2.p69-74

Abstract

Homalomena pexa, a tomentose haired-leaf aroid, was a newly discovered and described plant species in early 2020. This species has currently only been reported from South Tapanuli. A molecular study to provide DNA sequence is essential in this preliminary investigation. This research aimed to characterize the barcode sequence of H. pexa and estimate the species' position in an Araceae phylogenetic tree. This research used ITS sequence to perform DNA barcoding on H. pexa. The sequencing result revealed that 1040 bp nucleotides were effectively amplified. The phylogenetic tree generated using the Neighbor-Joining method and the Kimura 2-parameter revealed that H. pexa clustered with H. atrox and H. humilis, with a bootstrap value of 97%. This investigation provided and demonstrated that ITS sequences could be used to validate and support the proper identification of Araceae species.
Effects of Zinc Accumulation on Earthworm Aporrectodea caliginosa (Haplotaxida: Lumbricidae) Mahmood, Safaa Mohammed; Mohammed, Adnan Musa
Jurnal Riset Biologi dan Aplikasinya Vol. 5 No. 1 (2023)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v5n1.p8-15

Abstract

Earthworms are key to the earth's ecosystem, which helps the soil increase its fertility and repair its existing elements, as well as remove contaminants. This study investigated the accumulation of Zn in the earthworm Aporrectodea caliginosa after 15 days of exposure. The worms were grown in media with different concentrations of Zn between 750 and 1500 ppm. Each treatment consisted of three replicates, containing 30 worms. A control group without Zn was also used. Data were analyzed by using Duncan Multi–Range. The results revealed that A. caliginosa had a strong ability to accumulate Zn in its tissue compared to the control group. It was noticed that the increase of the heavy metal in the worm´s tissue is associated with the elevation of the metal in its media. The results show a significant loss of weight in the worm´s body and loss in the growth rate; they also shown a decrease in specific growth rate. Moreover, there was a significant decrease in the worm length, showing a high effect, especially after 15 days of breeding with all concentrations used in this study. The researchers recommend using earthworms to purify the soil from contaminants because earthworm has a great ability to get rid of all pollutants, whether metals or pesticides or parasites, especially in industrialized countries and agricultural lands.
A Genetic Identification of Superbugs from River Streams in State University of Malang Sholekah, Reeno Al Hikmatus; Listyorini, Dwi
Jurnal Riset Biologi dan Aplikasinya Vol. 5 No. 1 (2023)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v5n1.p27-36

Abstract

Superbugs revert to bacterial strains, which exhibit resistance to antibiotics. These bacteria could cause some problems in disease treatment and the environment. This research aimed of this study was to identify the presence of superbugs in the river stream that flows through Malang State University campus area. Amoxicillin, tetracycline, and chloramphenicol were selected to test the possible bacterial resistance. Water samples taken from a decided spot were spread over Luria Bertani’s agar on both antibiotic supplemented and antibiotics-free media with no delay. Molecular identification was carried out using 16S rRNA gene DNA barcoding approach, completed with morphological and Gram staining analyses. A total of 16 isolates of gram-negative colonies were found in the form of bacilli, diplobacilli, cocci, and diplococci. The genetic identification of eight resistant colonies led us to suggest that the isolates may belong to Aeromonas, Shigella, and Bacillus. Further studies are still required to get a clearer view of the correct taxonomical position of those resistant isolates.
Diversity of Moss Species (Bryophyta) In Senggani Ravine Tourism Area, Tulungagung Regency Kartikasari, Desi; Anon Widodo, Gading; Habibah, Nur; Zahratul Asna, Rakhmi
Jurnal Riset Biologi dan Aplikasinya Vol. 5 No. 1 (2023)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v5n1.p43-51

Abstract

Moss plants (Bryophyta) are found in every habitat, and their presence in an ecosystem is controlled by environmental circumstances. The Senggani Ravine tourism area is a popular tourist attraction comprising a pine forest with extensive moss communities. This work aimed to assess the diversity of mosses (Bryophyta) in the Senggani Ravine tourism area for the first time. In June 2022, exploratory descriptive research of moss diversity was undertaken by a free walk around the Senggani Ravine tourism area from a predetermined position point (purposive sampling) using observation, documentation, literature study, and measurement of abiotic elements for data collection. Based on the results, twenty species of mosses were identified and can be divided into four classes, namely the Bryopsida, Polytrichopsida, Jungermanniopsida, and Marchantiopsida. The species identified were Barbulla indica, Fissidens purpusillus, Fissidens biformis, Fissidens biformis, Octoblepharum albidum, Rhizonium punctatum, Mnium hornum, Philonotis marchica, Fontinalis antipyretica, Hypnum cupressiform, Polytrichastrum formosum, Lejeunea flava, Lejeunea cavifolia, Bazzania prareupta, Bazzania vittata, Riccia junghuhniana, Marchantia emarginata, Marchantia polymorpha, Dumortiera hirsuta, and Lunularia cruciate. Abiotic factor measurements revealed that zone 3, which has a soil pH of 6, an air temperature of 24.1 C°, an 84% humidity level, and 200 Cd of light cm-1, is the most favorable area for moss growth. We can infer that the Senggani Ravine tourism area is still primarily undisturbed because the moss flora is still quite diverse and varied.
Leaf and Stomata Morphometrics of Gayam Inocarpus fagifer (Fabaceae) at Different Altitudes Smith, Alwi; Sangur, Kristin; Molle, Dessy Fitri; Haurissa, Ludia; Maulany, Grisendy; Renyaan, Belsefren
Jurnal Riset Biologi dan Aplikasinya Vol. 5 No. 1 (2023)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v5n1.p16-26

Abstract

Gayam (Inocarpus fagifer) is one of the members of the angiosperm flora in Ambon City, Indonesia, that grows and develops at various altitudes. This research aimed to analyze the leaf and stomata morphometrics of these plants in the Aer Louw and Ema Village areas. Leaf samples were taken from the upper, middle, and lower strata and considered as replicates. The morphometric characteristics were measured manually using millimeter block paper and the formula for calculating leaf ratio. Furthermore, the stomata were stained using the direct incision method and safranin. The incision results were analyzed using an Olympus CX23 microscope at 400x magnification. The measurement and observation were analyzed descriptively and correlatively. The results showed that the average leaf width and length, also the midrib length were greater in Aer Louw Village than in Ema Village; while the leaf tip and stalk length were greater in Ema Village than in Aer Louw Village. The characteristics of stomata length and width in Ema Village were greater than in Aer Louw Village; otherwise, the number, index, and density of stomata in Aer Louw Village were greater than in Ema Village. Meanwhile, the correlational analysis showed that the environment influenced the variations of leaves and stomata. Therefore, the variations of leaves and stomata in the areas could predict plant adaptations to different environments.

Page 9 of 13 | Total Record : 126

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