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IJAAM (Indonesian Journal of Anti-Aging Medicine)
Published by Universitas Udayana
ISSN : 26147882     EISSN : 26155079     DOI : https://doi.org/10.36675/ijaam
Core Subject : Health,
Medicine & Pharmacology
Indonesian Journal of Anti-Aging Medicine is published by Faculty of Medicine Universitas Udayana in collaboration with Indonesian Center for Anti-Aging Medicine (INCAAM). Indonesian Journal of Anti-Aging Medicine is an open access, peer reviewed journal aiming to communicate high quality research articles, reviews and general articles in the field of anti aging. Indonesian Journal of Anti-Aging Medicine encompass all aspects of basic research/clinical studies related to the field of anti aging and allied science fileds. The Journal aims to bridge and integrate the intellectual, methodological, and substantive diversity of medical scholarship, and to encourage a vigorous dialogue between medical scholars and practitioners. The Journal welcomes contributions which promote the exchange of ideas and rational discourse between practicing educators and medical researchers all over the world.
Arjuna Subject : Kedokteran - Kedokteran (Lain-Lain)
Articles 73 Documents
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Administration of Ashitaba (Angelica Keiskei) leaves extracts prevented Superoxide Dismutase (SOD) Reduction and Malondialdehyde (MDA) elevation in overtrained-male wistar rats (Rattus norvegicus) Ronald Suryaprawira; Alex Pangkahila; Wimpie Pangkahila
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 5 No 1 (2021): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v5i1.63

Abstract

Introduction: The aging process is caused by several factors, one of which is the accumulation of oxidative damage caused by high free radicals in the body. Ashitaba (Angelica keiskei) leaves extract contains flavonoids, polyphenols, tannins, and antioxidant capacity. This study aimed to prove that administration of Ashitaba leaves extracts prevent superoxide dismutase (SOD) reduction and malondialdehyde (MDA) elevation in overtraining male Wistar rats (Rattus norvegicus). Methods: This study was an experimental study with randomized pretest-posttest control group design. The subjects used in this study were 16 male Wistar rats, healthy, aged 6 months, and weighing 180- 200 grams. The control group was given overtraining two hours after aquabidest 2 cc treatment (P0), and the treatment group was given overtraining two hours after administration of Ashitaba leaves extract of 20 mg/kg BW/day (P1). Before and after treatment for 14 days, blood sample were drawn through canthus medial orbital sinus to measure the level of SOD and MDA using the ELISA method. Results: The results showed in control group there was a decrease in SOD levels (from 8.58±0.78 ng/mL to 5.93±1.27 ng/mL; p<0.001) and increased MDA levels (from 2.75±0.19 nmol/mL to 3.28±0.23 nmol/mL; p= 0.001). In treatment group there was no change in SOD levels (from 8.90±0.61 ng/mL to 8.57±0.49 ng/mL; p= 0.217) and there was no change in MDA levels (from 2.67±0.33 nmol/mL to 2.62±0.24 nmol/mL; p= 0.693). Conclusion: It can be concluded that administration of Ashitaba leaves extracts prevented SOD reduction and MDA elevation in overtraining male Wistar rats.
Administration of Ashitaba (Angelica keiskei) Leaf Extracts Cream Prevented The Increase of MMP-1 Expression and The Decrease of Collagen Number in The Male Wistar Rats (Rattus norvegicus) Skin Exposed to UVB Phery Candres; Wimpie Pangkahila; Anak Agung Gede Putra Wiraguna
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 4 No 2 (2020): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v4i2.102

Abstract

Introduction: Ultraviolet, especially the ultraviolet B (UV-B), is the most dominant cause of skin aging through increasing collagen degradation and activation of matrix metalloproteinase (MMPs). Recently, a lot of research focus on finding natural antioxidants with photoprotective activity. Ashitaba (Angelica keiskei) contains antioxidants and chalcone flavonoids. The purpose of this study was to examine the effect of ashitaba leaf extract, whether it could prevent increasing MMP-1 expression and decreasing collagen amount in rats exposed to UV-B rays. Methods: This study used randomized posttest only control group design. Subjects were 36 albino rats (Rattus norvegicus), male, adult (2.5 - 3 months old), weighing between 180-200 grams. All rats were divided into 18 rats each, namely the control group (the subject was smeared with basic cream and exposed to UVB) and the treatment group (the subject was smeared with ashitaba extract cream and exposed to UVB). The expression of MMP-1 using immunohistochemical staining and the amount of collagen using Sirius red staining. Results: The mean expression of MMP-1 in the control group was 28.14 ± 2.89% and in the treatment group was statistically lower at 9.45 ± 2.67% (p <0.001). The average amount of collagen in the control group was 53.64 ± 2.58% and in the treatment group was statistically higher at 83.57 ± 2.47% (p <0.001). Conclusion: The administration of ashitaba (Angelica keiskei) leaf extracts cream prevented the increase of MMP-1 expression and the decrease of collagen number in the male wistar rats (Rattus norvegicus) skin exposed to ultraviolet B.
Ethanol extract of red fruit (Pandanus conoideus lam.) improved lipid profile of dyslipidemic-male wistar rats (Rattus norvegicus) Sriwiyanti Sriwiyanti; I Gusti Made Aman; Gde Ngurah Indraguna Pinatih
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 5 No 2 (2021): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v5i2.88

Abstract

Introduction: Dyslipidemia is one of aging- related disease associated with unhealthy food such as high calories and fat. Pandanus Conoideus Lam. with high antioxidant capacity might ameliorates dyslipidemia phenotypes. This study aimed to prove that ethanol extract of red fruit improves lipid profiles of dyslipidemic-rats (Rattus norvegicus). Methods: This was an experimental study with a pretest-posttest control group design, using 14 wistar rats males. Rats were then divided into 2 groups (n= 7). The first group was treated with aquabidestilata 1 mL for 30 days (P0). The second group was treated with extract of red fruit ethanol of 168 mg/200 gr BW for 30 days (P1). Results: The control group (P0) treated with aquabidestilata, total cholesterol levels increased into 221.48 ± 3.64 mg/dl (p <0.05), triglyceride levels elevated into 132.92±3.01 mg/dl (p <0.05), HDL levels depleted into 22.83 ± 2.26 mg/dl (p <0.05), and LDL levels was increased from 76.77 ± 3.33 mg / dl to 78.63 ± 2.84 mg / dl (p <0.05). In contrast, the red fruit ethanol extract in treatment group (P1) experienced decrease on total cholesterol level into 118.41 ± 2.15 mg/dl (p <0.05), triglyceride levels into 91.81 ± 2.71 mg/dl (p <0.05), and LDL levels into 45.85 ± 2.59 mg/dl (p <0.05), and elevation on HDL levels from 25.77 ± 1.36 mg/dl to 55.76 ± 2.35 mg/dl (p <0.05). Conclusion: This study indicated that administration of ethanol extract of red fruit (Pandanus conoideus lam.) improved lipid profiles of dyslipidemic-rats (Rattus norvegicus).
Administration of Moringa (Moringa oleifera) Fruit Seed Extract Cream 6% Prevented Collagen Depletion in Ultraviolet B-Induced Guinea Pigs (Cavia Porcelus) Skin Yuziana Yuziana; Wimpie Pangkahila; Anak Agung Gede Putra Wiraguna
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 5 No 1 (2021): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v5i1.74

Abstract

ABSTRACT Background: Skin is often exposed to the environment such as Ultraviolet (UV) radiation which causes skin aging or photoaging. UVB-induced photoaging is characterized by a decrease in the amount of collagen. Natural ingredients that contain antioxidants, such as Moringa fruit seed extract containing bioactive compounds, are expected to prevent a decrease in the amount of collagen that eventually slows skin aging. The purpose of this study was to prove that administration of Moringa (Moringa oleifera) fruit seed extract cream 6% prevents collagen depletion in ultraviolet B-induced guinea pigs (Cavia porcelus) skin. Methods: This study was an experimental study with post-test only control group design. The subjects used in this study were 36 guinea pig (Cavia porcelus), 3 months old, male, and weighing 300-350 gr. The guinea pigs were grouped randomly into 2 groups with 18 guinea pigs/group. The first group (control) was the group treated with basic cream and exposed to UVB rays of 840 mJ/cm2; while the second group (treatment group) was treated with Moringa fruit seed extract cream 6% and exposed to UVB rays of 840 mJ/cm2. After 28 days of treatment, the guinea pigs were euthanized and histopathological examination of collagen was carried out with Sirius red staining. Results: The results showed that the average amount of collagen in the control group was 59.73 ± 2.31%. In the group of Moringa fruit seed extract cream 6%, the collagen amount was 84.07 ± 2.03%. Significance analysis using independent t-test showed a p value of <0.001 which means that there was a difference in the average amount of collagen between groups. Conclusion: In this study, the results indicated that administration of Moringa (Moringa oleifera) fruit seed extract cream 6% prevented collagen depletion in ultraviolet B-induced guinea pigs (Cavia porcelus) skin. Keywords: Moringa fruit seeds, collagen, guinea pigs, ultraviolet B.
Oral Administration of Polypodium leucotomos Powder Inhibited Sunburn Cells Formation in UVB-Induced Male Wistar Rats (Rattus Norvegicus) Priskila Ariana; I Gusti Made Aman; Anak Agung Gede Putra Wiraguna
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 5 No 1 (2021): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v5i1.59

Abstract

Introduction: UVB can cause direct damage to the DNA and eventually cause apoptosis of epidermal keratinocytes, which is called a sunburn cells. Polypodium leucotomos (Heliocare®) is one of the skin photoprotectors that can modulate immune and inflammatory responses that play a role in preventing sunburn. This study aimed to prove that oral administration of Polypodium leucotomos powder inhibits sunburn cells formation in UVB-induced male Wistar rats (Rattus norvegicus). Methods: This study was a true experimental study using post-test only control group design consisted of two groups with 18 rats/group. One group as a control group (P0) that was treated with oral 1ml aquadest (force feeding) for 10 days. The other group was the treatment group (P1) treated with oral Heliocare® dry powder (Polypodium leucotomos) of 80 mg/kg BW diluted in 1 ml aquadest (force feeding) for 10 days. On the day 11, the dorsal skin was shaved and exposed to single dose UVB of 25mJ. Twenty-four hours later, rats were sacrificed and skin samples were collected for histology preparations with Hematoxylin-Eosin staining and sunburn cells were counted as posttest data. Results: The results showed that the average number of sunburn cells in the P0 group was 36.5±7.53 sunburn cells/mm2, while in the P1 group was 8.3±3.59 sunburn cells/mm2 (p <0.05). Those data showed that there was a significant different of average number of sunburn cells among group tested. Conclusion: It can be concluded that oral administration of 80 mg/kg BW Polypodium leucotomos powder inhibited sunburn cells formation in UVB-induced male Wistar rats (Rattus norvegicus).
Awarding of green coffee bean (Coffea arabica) extract improved lipid profile in dyslypidemic male wistar rats (Rattus norvegicus) Fibriany Chandra; I Wayan Weta; Ni Nyoman Ayu Dewi
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 5 No 2 (2021): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v5i2.83

Abstract

Introduction: Dyslipidemia is a lipid metabolic disorder characterized by increased or decreased lipid fraction in plasma. The active compounds of chlorogenic acid in coffee can increases the body’s metabolism, increases fatty acid oxidation, decreases triglyceride levels in the liver, and inhibit the action of the enzyme amylase and lipase pancreas in the intestinal. This study aimed to prove the effectiveness of green coffee bean extract in improving serum lipid profile levels in dyslipidemic male Wistar rats. Methods: The research design was experimental pre-post test control group using 14 male Wistar rats. The control group was given placebo (2 cc aquades), while the second group was treated with ethanol extract of green beans dose 200 mg/kg dissolved in 2 cc aquades given sonde with treatment time of 14 days. On the first and fifteenth days, blood sampling is done to check the lipid profile level. Results: The results showed that the mean total cholesterol level of the control group was 137.86 ± 10.12 mg/dL while the total cholesterol level in the treatment group was 137.86 ± 10.12 mg/dL. The mean of triglyceride level, LDL level, and HDL level control group was 100.86 ± 8.98 mg/dL, 97.57 ± 4.65 mg/dL, and 51.57 ± 4.96 mg/dL while triglyceride level, LDL level, and HDL level in treatment group was 76.86 ± 4.94 mg/dL, 82.86 ± 5.21 mg/dL and 66.43 ± 8.84 mg/dL. Conclusion: Arabica green coffee bean (Coffea arabica) extract improves the lipid profile of dyslipidemic male Wistar rats (Rattus norvegicus).
Supplementation of Sauropus androgynus (L.) Merr leaves extract reduced more body weight and triglyceride in obese male wistar rats with moderate exercise Christine Icasia; Alex Pangkahila; I Wayan Weta
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 4 No 2 (2020): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v4i2.94

Abstract

Introduction: Moderate exercise is one of the pillars of weight management. Supplementation of ingredients containing bioactive compounds such as Sauropus androgynus (L.) Merr leaves extract which contains flavonoids, tannins, and saponins may help obesity management. The purpose of this study was to prove that supplementation of Sauropus androgynus (L.) Merr leaves extract reduced more body weight and triglyceride in obese male Wistar rats with moderate exercise. Methods: This research was an experimental study with a randomized pretest-posttest control group design. The subjects were 20 rats (Rattus norvegicus). Obesity and hypertriglyceridemia were induced by a high-fat and high-carbohydrate diet. Rats were divided into two groups, namely the control group and the treatment group. Results: The control group there was a decrease in body weight (from 284.5±3.17 to 278.3±4.27 grams; p <0.001) and a decrease in triglyceride levels (from 186.7±14.8 to 181.1±13.6; p = 0.047). In the treatment group there was also a decrease in body weight (from 282.1±5.72 to 272.2±3.55 grams; p <0.001) and a decrease in triglyceride levels (from 185.4±9.24 to 160.2±22.5; p = 0.003). The decrease in body weight in the treatment group was more than in control group (-9.90±3.37 vs -6.20±2.74; p = 0.018). Triglyceride levels in treatment group also decreased more than in control group (-25.2±19.3 vs -5.60±8.13; p = 0.008). Conclusion: Based on the results of this study, it can be concluded that supplementation of Sauropus androgynus (L.) Merr leaves extract reduced more body weight and triglyceride in obese male Wistar rats with moderate exercise.
Selenium enriched eggs is more effective to increase serum glutathione peroxidase and decrease F2-isoprostane level of excessive physical training-induced rats (Rattus norvegicus) than ordinary eggs Felix Kusmana; I Gusti Made Aman; Gde Ngurah Indraguna Pinatih
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 5 No 2 (2021): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v5i2.79

Abstract

Abstract Background: Exercise maintains health and prevent aging. However, excessive exercise causes overtraining syndrome. Selenium supplementation may increase cellular antioxidant activity, such as glutathione peroxidase (GPx), thioredoxin reductase (TRx), and selenoprotein P. This study aimed to prove that selenium-enriched eggs is more effective to increase serum glutathione peroxidase and decrease F2-isoprostane level of excessive physical training-induced rats (Rattus norvegicus) than ordinary eggs.Methods: This experimental study used randomized pretest-posttest control group design. Subjects were 16 male Wistar rats, 16 weeks old, healthy, weighing 150-160 grams. Subjects were divided randomly into 2 groups (n=8), one group was treated with excessive physical training and 6.48 grams of ordinary eggs, and another group was treated with excessive physical training and 6.48 grams of selenium-enriched eggs for 14 days. Results: Post-test analysis showed that the mean of glutathione peroxidase level in the group given ordinary eggs was 45.90±8.11 mIU/mL, while the group given selenium-enriched eggs was 72.61±7.40 mIU/mL (p= 0.001). In addition, the mean of F2-Isoprostane level in the group given ordinary eggs was 6.37±1.34 ng/ml and the group given selenium-enriched eggs was 3.39±0.77 ng/ml (p= 0,001). Analysis of paired sample t-test revealed that in both groups, glutathione peroxidase levels were increase while F2-isoprostane levels were decrease. Conclusion: This study suggested that selenium-enriched eggs is more effective to increase serum glutathione peroxidase and decrease F2-isoprostane level of excessive physical training-induced rats (Rattus norvegicus) than ordinary eggs. Key words: excessive physical training, F2-isoprostane, glutathione peroxidase, selenium-enriched eggs, Wistar rat.
Ashitaba (Angelica keiskei) leaves extract prevents spatial memory impairment in male balb/c mice (Mus musculus) with brain aging Indah Mira Tiaraputri Wijaya; Wimpie Pangkahila; Thomas Eko Purwata
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 5 No 1 (2021): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v5i1.64

Abstract

Background: Memory impairment is one of the symptoms of aging process which can be caused by free radicals or oxidative stress. Antioxidants such as flavonoids are compounds that can inhibit reactive oxygen species (ROS). Ashitaba (Angelica keiskei) is one of the natural compound that contains high flavonoids. The purpose of this study was to prove that Ashitaba (Angelica keiskei) leaves extract prevents spatial memory impairment in male Balb/c mice (Mus musculus) with brain aging Methods: Experimental randomized pretest-posttest group using 26 mice, aged 3 months, healthy (active and willing to eat), weighing 20-30 grams. Mice were induced with oral D-Galactose of 300 mg/kgBW/day for 4 consecutive weeks to achieve the brain aging condition. Control group treated with placebo (2 ml of aquabidest), and another group was treated with Ashitaba leaves extract of 20 mg/kgBW/day. Before and after treatment for 28 days, spatial memory were examined using the Morris Water Maze method. Results: The results showed a significant decrease in probe test (from 72,9±5,69% to 40,8±5,10%; p<0,001) and the number of platform crossings (from 7,37±0,527 times to 4,50 ± 0,771 times; p<0,001) in the control group suggesting that D-Galactose-only treatment impaired spatial memory. In contrast, the group treated with D-Galactose and Ashitaba leaves extract, there was no change in probe test (from 72,1±5,9% to 66,0±6,72%; p>0,05) and number of platform crossings (from 7,65±0,881 times to 6,98±0,787 times; p>0,05). Conclusion: This study indicated that Ashitaba (Angelica keiskei) leaves extracts prevented spatial memory impairment in male Balb/c mice (Mus musculus) with brain aging.
Administration of 5% Tranexamic Acid Cream Reduce the Amount of Melanin as Effective as 4% Hydroquinone Cream in Female Guinea Pigs (Cavia porcellus) Exposed to Ultraviolet-B Light Nina Marini; Wimpie Pangkahila; AAGP. Wiraguna
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 5 No 1 (2021): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v5i1.31

Abstract

Introduction: Exposure to ultraviolet B light will increase the amount of melanin which is a common sign of skin aging, one of which is hyperpigmentation which causes psychological impacts, causing social disturbances. Currently, no effective treatment has been found, where hydroquinone as the gold standard provides dangerous side effects, so a more effective and safe material is needed for long-term use. The aim of this study was to prove that tranexamic acid cream was as effective as hydroquinone in reducing the amount of melanin in female guinea pigs (Cavia porcellus) exposed to UVB rays. Methods: This study used a post test only control group design with 36 adult female guinea pigs exposed to UVB rays for 6 weeks at 60mJ/cm2 three times a week. After 2 weeks the guinea pigs were randomly divided into 2 groups with 18 guinea pigs in each group. Group A was given 4% hydroquinone cream and group B was given tranexamic acid cream 5%. Exposure to UVB rays was continued as long as the guinea pig was given the cream treatment. After 42 days the guinea pigs were biopsied to determine the amount of epidermal melanin. Results: The results showed that the mean amount of melanin in the hydroquinone group was 7.40 ± 3.61 and in the tranexamic acid group was 7.27 ± 4.06 (p=0.925; p>0.05). There was no significant difference between the hydroquinone cream group and the tranexamic sour cream group. Conclusion: This study concluded that administration of 5% tranexamic acid cream reduced the amount of melanin as effective as 4% hydroquinone cream in female guinea pigs exposed to UVB light.

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